2005
Symplekin and multiple other polyadenylation factors participate in 3′-end maturation of histone mRNAs
Kolev NG, Steitz JA. Symplekin and multiple other polyadenylation factors participate in 3′-end maturation of histone mRNAs. Genes & Development 2005, 19: 2583-2592. PMID: 16230528, PMCID: PMC1276732, DOI: 10.1101/gad.1371105.Peer-Reviewed Original ResearchConceptsTail elongationU7 small nuclear ribonucleoproteinCommon molecular machineryMammalian cell extractsCleavage stimulation factorPolyadenylation specificity factorSmall nuclear ribonucleoproteinMolecular machineryHistone mRNAProtein complexesMRNA cleavageSpecificity factorPolyadenylation factorsTranslational activationNuclear ribonucleoproteinSymplekinReconstitution experimentsCell extractsHeat-labile factorMessenger RNAHistonesMRNAStimulation factorSubunitsCytoplasmic
2001
Communication of the Position of Exon-Exon Junctions to the mRNA Surveillance Machinery by the Protein RNPS1
Lykke-Andersen J, Shu M, Steitz J. Communication of the Position of Exon-Exon Junctions to the mRNA Surveillance Machinery by the Protein RNPS1. Science 2001, 293: 1836-1839. PMID: 11546874, DOI: 10.1126/science.1062786.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsAnimalsCell LineDNA-Binding ProteinsExonsFungal ProteinsGlobinsHeLa CellsHumansMacromolecular SubstancesMiceModels, BiologicalPrecipitin TestsProtein BindingRecombinant Fusion ProteinsRibonucleoproteinsRNA HelicasesRNA SplicingRNA, MessengerRNA-Binding ProteinsSaccharomyces cerevisiae ProteinsTrans-ActivatorsTransfectionConceptsNonsense-mediated decayExon-exon junctionsMRNA surveillanceMRNA quality controlMRNA surveillance machinerySelective nuclear exportBeta-globin mRNAPremature termination codonUpf complexMature mRNASurveillance machineryNuclear exportAberrant mRNAsMammalian cellsTermination codonUntranslated regionSplice junctionsRNPS1MRNADual roleCentral componentComplexesCodonSubunitsMachinery
1988
A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells.
Steitz JA, Berg C, Hendrick JP, La Branche-Chabot H, Metspalu A, Rinke J, Yario T. A 5S rRNA/L5 complex is a precursor to ribosome assembly in mammalian cells. Journal Of Cell Biology 1988, 106: 545-556. PMID: 3279045, PMCID: PMC2115095, DOI: 10.1083/jcb.106.3.545.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutoantibodiesCell NucleolusCentrifugation, Density GradientElectrophoresis, Polyacrylamide GelFluorescent Antibody TechniqueFriend murine leukemia virusHeLa CellsHumansImmunoassayLeukemia, Erythroblastic, AcuteRibonucleoproteinsRibosomal ProteinsRibosomesRNA PrecursorsRNA, RibosomalRNA, Ribosomal, 5STumor Cells, CulturedConceptsRNA-protein complexesVivo pulse-chase experimentsRibosomal protein L5Pulse-chase experimentsProtein complexesProtein L5Mammalian cellsRNA moleculesMouse cellsLater stepsHeLa cellsProtein moietyRNPRibosomesNucleoliCellsComplexesAssemblyBiogenesisRRNAIndirect immunofluorescenceSubunitsRNAImmunofluorescence