1995
Modulation of 5' splice site choice in pre-messenger RNA by two distinct steps.
Tarn WY, Steitz JA. Modulation of 5' splice site choice in pre-messenger RNA by two distinct steps. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 2504-2508. PMID: 7708674, PMCID: PMC42246, DOI: 10.1073/pnas.92.7.2504.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinU1 small nuclear ribonucleoproteinSplice site choicePre-messenger RNASR proteinsSplice site selectionSplice siteSite choiceSer/Arg-rich (SR) proteinsU2 small nuclear ribonucleoproteinU1 snRNP functionsHeLa cell nuclear extractsEssential splicing factorArg-rich proteinsCell nuclear extractsDistinct stepsSnRNP functionSite selectionSplicing factorsU1 bindingNuclear ribonucleoproteinU1 RNANuclear extractsProteinRNA
1994
SR proteins can compensate for the loss of U1 snRNP functions in vitro.
Tarn WY, Steitz JA. SR proteins can compensate for the loss of U1 snRNP functions in vitro. Genes & Development 1994, 8: 2704-2717. PMID: 7958927, DOI: 10.1101/gad.8.22.2704.Peer-Reviewed Original ResearchConceptsSR proteinsSplice site recognitionSplice siteU1 snRNPsU1 snRNP functionsEssential splicing factorPre-mRNA substrateSplice site choiceNative gel analysisSplice site selectionMethyl oligoribonucleotideCross-linking studiesSnRNP functionSplicing factorsU1 snRNPU1 snRNASite recognitionSite choiceGel analysisRescue splicingProteinSplicing systemIntronsSnRNPs
1993
Uncoupling Two Functions of the U1 Small Nuclear Ribonucleoprotein Particle During in Vitro Splicing
Seiwert S, Steitz J. Uncoupling Two Functions of the U1 Small Nuclear Ribonucleoprotein Particle During in Vitro Splicing. Molecular And Cellular Biology 1993, 13: 3135-3145. DOI: 10.1128/mcb.13.6.3135-3145.1993.Peer-Reviewed Original ResearchSmall nuclear ribonucleoprotein particleSplice site recognitionU1 snRNASL RNASplice site regionSplicing substrateSpliced leader RNA sequencesVitro splicingSplice siteU1 snRNP functionsU1 small nuclear ribonucleoprotein particleEssential splicing factorLeader RNA sequencesNative gel analysisNuclear ribonucleoprotein particleSnRNP functionCaenorhabditis elegansSplicing complexesSplicing factorsLeptomonas collosomaSpliceosome assemblyDifferent intronsRibonucleoprotein particleU1 RNAMethyl oligoribonucleotideUncoupling two functions of the U1 small nuclear ribonucleoprotein particle during in vitro splicing.
Seiwert SD, Steitz JA. Uncoupling two functions of the U1 small nuclear ribonucleoprotein particle during in vitro splicing. Molecular And Cellular Biology 1993, 13: 3135-3145. PMID: 7684489, PMCID: PMC359749, DOI: 10.1128/mcb.13.6.3135.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsBase SequenceCaenorhabditis elegansCell NucleusExonsHeLa CellsHumansMolecular Sequence DataNucleic Acid ConformationOligodeoxyribonucleotidesPlasmidsRibonucleoprotein, U1 Small NuclearRNARNA SplicingRNA, Small NuclearSequence Homology, Nucleic AcidTranscription, GeneticTrypanosomatinaConceptsSmall nuclear ribonucleoprotein particleU1 small nuclear ribonucleoprotein particleNuclear ribonucleoprotein particleSplice site recognitionU1 snRNASL RNASplice site regionSplicing substrateRibonucleoprotein particleSpliced leader RNA sequencesVitro splicingSplice siteU1 snRNP functionsEssential splicing factorLeader RNA sequencesNative gel analysisSnRNP functionCaenorhabditis elegansSplicing complexesSplicing factorsLeptomonas collosomaSpliceosome assemblyDifferent intronsU1 RNAMethyl oligoribonucleotide