1997
A new strategy for introducing photoactivatable 4-thiouridine ((4S)U) into specific positions in a long RNA molecule.
Yu YT, Steitz JA. A new strategy for introducing photoactivatable 4-thiouridine ((4S)U) into specific positions in a long RNA molecule. RNA 1997, 3: 807-10. PMID: 9214662, PMCID: PMC1369526.Peer-Reviewed Original ResearchMeSH KeywordsAffinity LabelsBiochemistryDNA-Directed RNA PolymerasesPolymerase Chain ReactionRibonuclease HRNAThiouridineTranscription, GeneticConceptsPre-mRNAPre-mRNA substrateAT-AC intronsPhage RNA polymeraseRNA-DNA chimerasFull-length RNALong RNA moleculesRNA polymeraseRNA moleculesT4 RNA ligaseT4 DNA ligaseRNA ligaseDNA ligaseRNARNase H cleavageLigaseSpecific sitesSpecific positionsIntronsPolymeraseChimerasNew strategyCleavageOligonucleotideH cleavage
1989
Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III.
Gottlieb E, Steitz JA. Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III. The EMBO Journal 1989, 8: 851-861. PMID: 2470590, PMCID: PMC400884, DOI: 10.1002/j.1460-2075.1989.tb03446.x.Peer-Reviewed Original ResearchMeSH KeywordsAutoantigensCells, CulturedDNA-Directed RNA PolymerasesHumansKineticsModels, BiologicalRibonucleoproteinsRNARNA Polymerase IIITranscription FactorsTranscription, GeneticConceptsRNA polymerase III transcriptionPolymerase III transcriptionRNA polymerase IIITranscription complexPolymerase IIILa proteinTranscription termination factorFull-length transcriptsTranscription terminationTermination factorRNA productsTranscription intermediatesTranscriptsTranscriptionProteinComplexesPolymeraseRegulatorAbsenceThe RNA binding protein La influences both the accuracy and the efficiency of RNA polymerase III transcription in vitro.
Gottlieb E, Steitz JA. The RNA binding protein La influences both the accuracy and the efficiency of RNA polymerase III transcription in vitro. The EMBO Journal 1989, 8: 841-850. PMID: 2498086, PMCID: PMC400883, DOI: 10.1002/j.1460-2075.1989.tb03445.x.Peer-Reviewed Original ResearchMeSH KeywordsAutoantigensCarrier ProteinsDNA-Directed RNA PolymerasesHeLa CellsHumansRibonucleoproteinsRNA Polymerase IIIRNA-Binding ProteinsTranscription FactorsTranscription, GeneticConceptsTranscription levelsNascent RNA polymerase III transcriptsRNA polymerase III transcriptionAbundant nuclear phosphoproteinRNA polymerase III transcriptsPolymerase III transcriptionPolymerase III transcriptsClass III genesHeLa cell extractsAction of LaTranscript lengthTermination signalNuclear phosphoproteinUridylate residuesLa proteinTranscription activityAutoantigen LaCell extractsTranscriptsRNAAbsence of LAMouse monoclonal antibodyTranscriptionPhosphoproteinGenes
1985
Association of the lupus antigen La with a subset of U6 snRNA molecules
Rinke J, Steitz J. Association of the lupus antigen La with a subset of U6 snRNA molecules. Nucleic Acids Research 1985, 13: 2617-2629. PMID: 2582364, PMCID: PMC341179, DOI: 10.1093/nar/13.7.2617.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigensAutoantigensBase SequenceCarcinoma, Ehrlich TumorDNA-Directed RNA PolymerasesHeLa CellsHumansMiceNucleic Acid HybridizationRibonucleoproteinsRNARNA, Small Nuclear
1983
The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro
Mount S, Pettersson I, Hinterberger M, Karmas A, Steitz J. The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro. Cell 1983, 33: 509-518. PMID: 6190573, DOI: 10.1016/0092-8674(83)90432-4.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceDNA-Directed RNA PolymerasesHumansNucleoproteinsRibonuclease T1RibonucleoproteinsRibonucleoproteins, Small NuclearRNARNA SplicingT-PhagesConceptsRNA-protein complexesSplice siteU1 snRNPGlobin RNAPartial proteinase K digestionU1 snRNPsMouse beta-globin geneBacteriophage T7 RNA polymeraseEucaryotic cell nucleusMessenger RNA splicingT7 RNA polymeraseRecombinant DNA clonesBeta-globin geneT1 ribonuclease digestionSplice site sequencesSmall intronsSnRNP proteinsRNA splicingSnRNP complexDNA clonesRNA polymeraseSnRNPSite sequenceRibonuclease digestionProteinase K digestion
1974
T7 Early messenger RNAs are the direct products of ribonuclease III cleavage
Rosenberg M, Kramer R, Steitz J. T7 Early messenger RNAs are the direct products of ribonuclease III cleavage. Journal Of Molecular Biology 1974, 89: 777-782. PMID: 4615167, DOI: 10.1016/0022-2836(74)90052-7.Peer-Reviewed Original ResearchMeSH KeywordsColiphagesDNA VirusesDNA-Directed RNA PolymerasesElectrophoresis, Polyacrylamide GelEscherichia coliRibonucleasesRNA, MessengerRNA, ViralTranscription, GeneticVirus ReplicationSpecific recognition of the isolated R17 replicase initiator region by R17 coat protein
STEITZ J. Specific recognition of the isolated R17 replicase initiator region by R17 coat protein. Nature 1974, 248: 223-225. PMID: 4819415, DOI: 10.1038/248223a0.Peer-Reviewed Original Research