2006
Epstein-Barr virus noncoding RNAs are confined to the nucleus, whereas their partner, the human La protein, undergoes nucleocytoplasmic shuttling
Fok V, Friend K, Steitz JA. Epstein-Barr virus noncoding RNAs are confined to the nucleus, whereas their partner, the human La protein, undergoes nucleocytoplasmic shuttling. Journal Of Cell Biology 2006, 173: 319-325. PMID: 16682524, PMCID: PMC2063832, DOI: 10.1083/jcb.200601026.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsAntibiotics, AntineoplasticAutoantigensCell LineCell Line, TumorCell NucleusDactinomycinFatty Acids, UnsaturatedFemaleHeLa CellsHerpesvirus 4, HumanHumansKaryopherinsMiceNIH 3T3 CellsOocytesProtein BindingRibonucleoproteinsRNA TransportRNA, UntranslatedRNA, ViralXenopus laevis
2001
Internal Modification of U2 Small Nuclear (Snrna) Occurs in Nucleoli of Xenopus Oocytes
Yu Y, Shu M, Narayanan A, Terns R, Terns M, Steitz J. Internal Modification of U2 Small Nuclear (Snrna) Occurs in Nucleoli of Xenopus Oocytes. Journal Of Cell Biology 2001, 152: 1279-1288. PMID: 11257127, PMCID: PMC2199211, DOI: 10.1083/jcb.152.6.1279.Peer-Reviewed Original ResearchConceptsNucleolar localizationCajal bodiesU2 RNAInternal modificationSmall nuclearSm binding siteNucleolar localization signalSmall nucleolar RNAsXenopus oocytesCytoplasm of oocytesU2 small nuclearGuanosine capLocalization signalNucleolar RNAsRNAs showSubcellular sitesIntranuclear localizationIsolated nucleiRNABinding sitesNucleoliOocytesNucleotidesCytoplasmU2
1989
Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III.
Gottlieb E, Steitz JA. Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III. The EMBO Journal 1989, 8: 851-861. PMID: 2470590, PMCID: PMC400884, DOI: 10.1002/j.1460-2075.1989.tb03446.x.Peer-Reviewed Original ResearchConceptsRNA polymerase III transcriptionPolymerase III transcriptionRNA polymerase IIITranscription complexPolymerase IIILa proteinTranscription termination factorFull-length transcriptsTranscription terminationTermination factorRNA productsTranscription intermediatesTranscriptsTranscriptionProteinComplexesPolymeraseRegulatorAbsenceThe RNA binding protein La influences both the accuracy and the efficiency of RNA polymerase III transcription in vitro.
Gottlieb E, Steitz JA. The RNA binding protein La influences both the accuracy and the efficiency of RNA polymerase III transcription in vitro. The EMBO Journal 1989, 8: 841-850. PMID: 2498086, PMCID: PMC400883, DOI: 10.1002/j.1460-2075.1989.tb03445.x.Peer-Reviewed Original ResearchConceptsTranscription levelsNascent RNA polymerase III transcriptsRNA polymerase III transcriptionAbundant nuclear phosphoproteinRNA polymerase III transcriptsPolymerase III transcriptionPolymerase III transcriptsClass III genesHeLa cell extractsAction of LaTranscript lengthTermination signalNuclear phosphoproteinUridylate residuesLa proteinTranscription activityAutoantigen LaCell extractsTranscriptsRNAAbsence of LAMouse monoclonal antibodyTranscriptionPhosphoproteinGenes
1988
Trans splicing involves a novel form of small nuclear ribonucleoprotein particles
Bruzik J, Doren K, Hirsh D, Steitz J. Trans splicing involves a novel form of small nuclear ribonucleoprotein particles. Nature 1988, 335: 559-562. PMID: 2971142, DOI: 10.1038/335559a0.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinSL RNACis splicingSplice siteNuclear ribonucleoproteinPrecursor-messenger RNA (pre-mRNA) transcriptsHeLa cell nuclear extractsSame nuclear compartmentTrans-splicing reactionCell nuclear extractsPossible secondary structuresMessenger RNA transcriptsSm snRNPSplice acceptor siteTrans splicingCellular machineryLeader transcriptNuclear compartmentNucleotide sequenceSplicing processRNA transcriptsNuclear extractsSnRNPSplicingSecondary structureStructural analyses of EBER1 and EBER2 ribonucleoprotein particles present in Epstein-Barr virus-infected cells
Glickman JN, Howe JG, Steitz JA. Structural analyses of EBER1 and EBER2 ribonucleoprotein particles present in Epstein-Barr virus-infected cells. Journal Of Virology 1988, 62: 902-911. PMID: 2828685, PMCID: PMC253649, DOI: 10.1128/jvi.62.3.902-911.1988.Peer-Reviewed Original Research
1987
Both conserved signals on mammalian histone pre-mRNAs associate with small nuclear ribonucleoproteins during 3' end formation in vitro.
Mowry KL, Steitz JA. Both conserved signals on mammalian histone pre-mRNAs associate with small nuclear ribonucleoproteins during 3' end formation in vitro. Molecular And Cellular Biology 1987, 7: 1663-1672. PMID: 2955216, PMCID: PMC365266, DOI: 10.1128/mcb.7.5.1663.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinEnd formationNuclear ribonucleoproteinSequence elementsSm small nuclear ribonucleoproteinsMouse histone genesHeLa cell nuclear extractsHistone H3 transcriptsHuman histone H3Trimethylguanosine cap structureCell nuclear extractsHistone pre-mRNAHairpin loop structureH3 transcriptsHistone genesMammalian histonesU RNAHistone H3MRNA substratesPre-mRNACap structureMRNA associatesNuclear extractsRNA fragmentsProcessing reactions
1985
Association of the lupus antigen La with a subset of U6 snRNA molecules
Rinke J, Steitz J. Association of the lupus antigen La with a subset of U6 snRNA molecules. Nucleic Acids Research 1985, 13: 2617-2629. PMID: 2582364, PMCID: PMC341179, DOI: 10.1093/nar/13.7.2617.Peer-Reviewed Original Research
1983
Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein
Padgett R, Mount S, Steitz J, Sharp P. Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein. Cell 1983, 35: 101-107. PMID: 6194895, DOI: 10.1016/0092-8674(83)90212-x.Peer-Reviewed Original ResearchGenes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genome
Wolin S, Steitz J. Genes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genome. Cell 1983, 32: 735-744. PMID: 6187471, DOI: 10.1016/0092-8674(83)90059-4.Peer-Reviewed Original ResearchConceptsHuman genomeRo RNAsSecondary structure homologyRNA polymerase IIISmall cytoplasmic ribonucleoproteinsClass III genesGenomic clonesMammalian cellsPolymerase IIIRNA componentStructure homologySingle copyCytoplasmic ribonucleoproteinHY5 RNAMouse cellsHuman cellsHY1Cell extractsGenesRNAGenomeRNAsHY3CellsMY1Sequential association of nucleolar 7-2 RNA with two different autoantigens.
Hashimoto C, Steitz JA. Sequential association of nucleolar 7-2 RNA with two different autoantigens. Journal Of Biological Chemistry 1983, 258: 1379-1382. PMID: 6185483, DOI: 10.1016/s0021-9258(18)32990-9.Peer-Reviewed Original Research
1982
New directions in antinuclear antibody research: the Sm, RNP, Ro, and La antigens are found on small-RNA protein particles.
Hardin JA, Lerner MR, Lerner EA, Steitz JA. New directions in antinuclear antibody research: the Sm, RNP, Ro, and La antigens are found on small-RNA protein particles. American Journal Of Kidney Diseases 1982, 2: 98-100. PMID: 6179414.Peer-Reviewed Original ResearchDeciphering the mysteries of RNA‐containing lupus antigens
Lerner E, Lerner M, Hardin J, Janeway C, Steitz J. Deciphering the mysteries of RNA‐containing lupus antigens. Arthritis & Rheumatism 1982, 25: 761-766. PMID: 6179522, DOI: 10.1002/art.1780250709.Peer-Reviewed Original ResearchPrecursor molecules of both human 5S ribosomal RNA and transfer RNAs are bound by a cellular protein reactive with anti-La Lupus antibodies
Rinke J, Steitz J. Precursor molecules of both human 5S ribosomal RNA and transfer RNAs are bound by a cellular protein reactive with anti-La Lupus antibodies. Cell 1982, 29: 149-159. PMID: 7105180, DOI: 10.1016/0092-8674(82)90099-x.Peer-Reviewed Original ResearchConceptsRRNA moleculesLa proteinLa RNAsPrecursor formNuclear transcription systemUninfected mammalian cellsPulse-chase experimentsSmall RNAsSmall ribonucleoproteinPrecursor moleculesTransfer RNAMammalian cellsRibosomal RNATRNA precursorsCertain tRNAsSpecific tRNAsRNA transcriptsTranscription systemU residuesCell extractsMature sizeHeLa cellsRNAEssential roleProtein
1981
Striking similarities are exhibited by two small Epstein-Barr virus-encoded ribonucleic acids and the adenovirus-associated ribonucleic acids VAI and VAII.
Rosa MD, Gottlieb E, Lerner MR, Steitz JA. Striking similarities are exhibited by two small Epstein-Barr virus-encoded ribonucleic acids and the adenovirus-associated ribonucleic acids VAI and VAII. Molecular And Cellular Biology 1981, 1: 785-796. PMID: 9279391, PMCID: PMC369362, DOI: 10.1128/mcb.1.9.785.Peer-Reviewed Original ResearchConceptsEBER-1EBER-2Epstein-Barr virus-encoded ribonucleic acidEBER genesEpstein-Barr virus genomeEBV deoxyribonucleic acidSystemic lupus erythematosusAnti-La antibodiesEcoRI J fragmentRibonucleic acidSmall ribonucleic acidsLupus erythematosusUninfected cellsEBERVirus genomeViral genomeErythematosusVaIITwo Novel Classes of Small Ribonucleoproteins Detected by Antibodies Associated with Lupus Erythematosus
Lerner M, Boyle J, Hardin J, Steitz J. Two Novel Classes of Small Ribonucleoproteins Detected by Antibodies Associated with Lupus Erythematosus. Science 1981, 211: 400-402. PMID: 6164096, DOI: 10.1126/science.6164096.Peer-Reviewed Original Research