2006
Multiple domains of EBER 1, an Epstein-Barr virus noncoding RNA, recruit human ribosomal protein L22
Fok V, Mitton-Fry RM, Grech A, Steitz JA. Multiple domains of EBER 1, an Epstein-Barr virus noncoding RNA, recruit human ribosomal protein L22. RNA 2006, 12: 872-882. PMID: 16556938, PMCID: PMC1440895, DOI: 10.1261/rna.2339606.Peer-Reviewed Original ResearchMeSH KeywordsBinding SitesCarrier ProteinsCell LineCross-Linking ReagentsElectrophoretic Mobility Shift AssayHerpesvirus 4, HumanHumansIn Vitro TechniquesMaltose-Binding ProteinsNucleic Acid ConformationPlasmidsProtein BindingProtein Structure, TertiaryRecombinant Fusion ProteinsRibosomal ProteinsRNA-Binding ProteinsRNA, UntranslatedRNA, ViralSequence DeletionTranscription, GeneticTransfectionUltraviolet Rays
2001
A novel embryonic poly(A) binding protein, ePAB, regulates mRNA deadenylation in Xenopus egg extracts
Voeltz GK, Ongkasuwan J, Standart N, Steitz JA. A novel embryonic poly(A) binding protein, ePAB, regulates mRNA deadenylation in Xenopus egg extracts. Genes & Development 2001, 15: 774-788. PMID: 11274061, PMCID: PMC312653, DOI: 10.1101/gad.872201.Peer-Reviewed Original ResearchAdenosine MonophosphateAmino Acid SequenceAnimalsBase SequenceBlotting, WesternDNA MethylationGene Expression Regulation, DevelopmentalKineticsMolecular Sequence DataOocytesPlasmidsPoly APoly(A)-Binding ProteinsPrecipitin TestsProtein BindingRecombinant ProteinsRNA-Binding ProteinsSequence Analysis, DNASequence Homology, Amino AcidTime FactorsTranscriptional ActivationUltraviolet RaysXenopusXenopus Proteins
1997
Site-specific crosslinking of mammalian U11 and U6atac to the 5′ splice site of an AT–AC intron
Yu Y, Steitz J. Site-specific crosslinking of mammalian U11 and U6atac to the 5′ splice site of an AT–AC intron. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 6030-6035. PMID: 9177163, PMCID: PMC20995, DOI: 10.1073/pnas.94.12.6030.Peer-Reviewed Original ResearchIdentification of HuR as a protein implicated in AUUUA‐mediated mRNA decay
Myer V, Fan X, Steitz J. Identification of HuR as a protein implicated in AUUUA‐mediated mRNA decay. The EMBO Journal 1997, 16: 2130-2139. PMID: 9155038, PMCID: PMC1169815, DOI: 10.1093/emboj/16.8.2130.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAmino Acid SequenceAnimalsAntigens, SurfaceBase CompositionCell ExtractsCross-Linking ReagentsELAV ProteinsELAV-Like Protein 1Gene Expression RegulationHeLa CellsHumansMiceMolecular Sequence DataMolecular WeightRegulatory Sequences, Nucleic AcidRNA-Binding ProteinsRNA, MessengerUltraviolet RaysConceptsAU-rich elementsMRNA decayUntranslated regionRNA-binding specificityARE-binding proteinsHeLa nuclear extractsGene familyMRNA degradationNuclear extractsEssential signalMessenger RNAProteinSequence's abilityHuRAUUUARapid degradationCritical roleHuR.RNAMachineryMRNADegradationRegulationSubsequent analysisExpression
1992
Viral small nuclear ribonucleoproteins bind a protein implicated in messenger RNA destabilization.
Myer VE, Lee SI, Steitz JA. Viral small nuclear ribonucleoproteins bind a protein implicated in messenger RNA destabilization. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 1296-1300. PMID: 1311093, PMCID: PMC48436, DOI: 10.1073/pnas.89.4.1296.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinNuclear ribonucleoproteinCertain cellular mRNAsMessenger RNA destabilizationHerpesvirus saimiriNumber of protooncogenesHSUR 1HSURs 1Rapid degradationSmall RNAsU RNARNA destabilizationCellular mRNAsMarmoset T lymphocytesAbundant viral transcriptHost proteinsUntranslated regionAUUUA motifsExhibit sequencesViral transcriptsPrimate virusesViral transformationUnstable messagesProteinRibonucleoprotein