2005
Small Nuclear RNAs Encoded by Herpesvirus saimiri Upregulate the Expression of Genes Linked to T Cell Activation in Virally Transformed T Cells
Cook HL, Lytle JR, Mischo HE, Li MJ, Rossi JJ, Silva DP, Desrosiers RC, Steitz JA. Small Nuclear RNAs Encoded by Herpesvirus saimiri Upregulate the Expression of Genes Linked to T Cell Activation in Virally Transformed T Cells. Current Biology 2005, 15: 974-979. PMID: 15916956, DOI: 10.1016/j.cub.2005.04.034.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDAntigens, Differentiation, T-LymphocyteAntigens, NeoplasmBase PairingBlotting, NorthernBlotting, WesternCallithrixCD52 AntigenCell Line, TumorFlow CytometryGenetic VectorsGenome, ViralGlycoproteinsHerpesvirus 2, SaimiriineLentivirusLymphocyte ActivationMembrane ProteinsMicroarray AnalysisOncogene Proteins, ViralReceptors, Antigen, T-CellReceptors, ImmunologicRNA, Small NuclearT-LymphocytesTransduction, GeneticUp-RegulationConceptsSmall nuclear RNAHSURs 1Herpesvirus saimiriNuclear RNAExpression of genesAggressive T-cell leukemiaMarmoset T cellsHSUR 1U RNAAbundant viral transcriptCell activationT cellsHost mRNAsUnexpected roleIntracellular proteinsNorthern analysisSm classHost targetsViral transcriptsT cell activationNew World primatesRNAT-cell receptor betaT-cell leukemiaGamma chain
2004
Guide RNAs with 5′ Caps and Novel Box C/D snoRNA-like Domains for Modification of snRNAs in Metazoa
Tycowski KT, Aab A, Steitz JA. Guide RNAs with 5′ Caps and Novel Box C/D snoRNA-like Domains for Modification of snRNAs in Metazoa. Current Biology 2004, 14: 1985-1995. PMID: 15556860, DOI: 10.1016/j.cub.2004.11.003.Peer-Reviewed Original ResearchConceptsModification guide RNAsGuide RNABox C/D snoRNAsInvariant G residueKink-turn structureGuide RNA genesShort guide RNASmall ribonucleoprotein particlesMetazoan organismsAncestral metazoanGuanosine capMetazoan cellsSingle intronD snoRNAsRNA genesSpliceosomal snRNAsTelomerase RNARibosomal RNASuch RNAsRibonucleoprotein particleMetazoansUpstream promoterIntronsG residuesCommon modification
1999
Initial recognition of U12-dependent introns requires both U11/5′ splice-site and U12/branchpoint interactions
Frilander M, Steitz J. Initial recognition of U12-dependent introns requires both U11/5′ splice-site and U12/branchpoint interactions. Genes & Development 1999, 13: 851-863. PMID: 10197985, PMCID: PMC316595, DOI: 10.1101/gad.13.7.851.Peer-Reviewed Original ResearchAdenoviridaeBlotting, NorthernDose-Response Relationship, DrugEvolution, MolecularFicusinHeLa CellsHeterogeneous-Nuclear RibonucleoproteinsHumansIntronsModels, GeneticOligonucleotidesRibonuclease HRibonucleoprotein, U4-U6 Small NuclearRibonucleoproteinsRibonucleoproteins, Small NuclearRNA SplicingTime Factors
1996
A Novel Spliceosome Containing U11, U12, and U5 snRNPs Excises a Minor Class (AT–AC) Intron In Vitro
Tarn W, Steitz J. A Novel Spliceosome Containing U11, U12, and U5 snRNPs Excises a Minor Class (AT–AC) Intron In Vitro. Cell 1996, 84: 801-811. PMID: 8625417, DOI: 10.1016/s0092-8674(00)81057-0.Peer-Reviewed Original ResearchMeSH KeywordsBase CompositionBase SequenceBlotting, NorthernCell NucleusHeLa CellsHumansMolecular Sequence DataNucleic Acid ConformationOligodeoxyribonucleotidesPlasmidsPolymerase Chain ReactionRibonuclease HRibonucleoprotein, U5 Small NuclearRibonucleoproteins, Small NuclearRNA PrecursorsRNA SplicingConceptsU5 small nuclear ribonucleoproteinSmall nuclear ribonucleoproteinU12 small nuclear ribonucleoproteinsMinor class intronsProtein coding genesPre-mRNA substrateNative gel electrophoresisCoding genesBranch site sequenceSplicing complexesNuclear ribonucleoproteinPre-mRNAP120 geneLariat intermediateSite sequenceIntronsHeLa cellsEssential roleSplicingGel electrophoresisBranch siteGenesU12Minor classU11A mammalian gene with introns instead of exons generating stable RNA products
Tycowski K, Shu M, Steitz J. A mammalian gene with introns instead of exons generating stable RNA products. Nature 1996, 379: 464-466. PMID: 8559254, DOI: 10.1038/379464a0.Peer-Reviewed Original ResearchConceptsProtein-coding genesRibosomal subunit assemblyRibosomal RNA transcriptionSmall nucleolar RNAsMammalian genesSnoRNA genesEukaryotic cellsProtein codingMature rRNANucleolar RNAsHost genesSubunit assemblyDifferent intronsRNA transcriptionRNA productsExtensive complementarityIntronsGenesSnoRNAsExonsRNARRNATranscriptionPolysomesMaturation1
1994
Requirement for Intron-Encoded U22 Small Nucleolar RNA in 18S Ribosomal RNA Maturation
Tycowski K, Shu M, Steitz J. Requirement for Intron-Encoded U22 Small Nucleolar RNA in 18S Ribosomal RNA Maturation. Science 1994, 266: 1558-1561. PMID: 7985025, DOI: 10.1126/science.7985025.Peer-Reviewed Original ResearchConceptsRibosomal RNASmall RNAsProtein-coding gene transcriptsRibosomal RNA maturationSmall nucleolar RNAsRNA maturationVertebrate cellsCellular functionsNucleolar RNAsHost genesIntron fragmentGene transcriptsRNAXenopus oocytesU22IntronsGenesTranscriptsNucleoliOocytesMaturationTargetingCellsFragmentsDepletion
1992
The low-abundance U11 and U12 small nuclear ribonucleoproteins (snRNPs) interact to form a two-snRNP complex.
Wassarman KM, Steitz JA. The low-abundance U11 and U12 small nuclear ribonucleoproteins (snRNPs) interact to form a two-snRNP complex. Molecular And Cellular Biology 1992, 12: 1276-1285. PMID: 1372090, PMCID: PMC369560, DOI: 10.1128/mcb.12.3.1276.Peer-Reviewed Original ResearchThree novel functional variants of human U5 small nuclear RNA.
Sontheimer EJ, Steitz JA. Three novel functional variants of human U5 small nuclear RNA. Molecular And Cellular Biology 1992, 12: 734-746. PMID: 1310151, PMCID: PMC364287, DOI: 10.1128/mcb.12.2.734.Peer-Reviewed Original ResearchConceptsU5 small nuclear RNASmall nuclear RNANuclear RNAHeLa cellsSmall nuclear ribonucleoprotein particleTri-snRNP complexOligonucleotide-directed RNase H cleavageNuclear ribonucleoprotein particleNovel functional variantsFull-length speciesAffinity-purified spliceosomesTrimethylguanosine capAlternative splicingShorter speciesRibonucleoprotein particleMinimal domainHeLa extractsPrimer extensionFunctional variantsHigh abundanceBase changesNorthern blottingAbundant formRNASpecies
1991
Structural analyses of the 7SK ribonucleoprotein (RNP), the most abundant human small RNP of unknown function.
Wassarman DA, Steitz JA. Structural analyses of the 7SK ribonucleoprotein (RNP), the most abundant human small RNP of unknown function. Molecular And Cellular Biology 1991, 11: 3432-3445. PMID: 1646389, PMCID: PMC361072, DOI: 10.1128/mcb.11.7.3432.Peer-Reviewed Original Research
1990
The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing
Kass S, Tyc K, Steitz J, Sollner-Webb B. The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing. Cell 1990, 60: 897-908. PMID: 2156625, DOI: 10.1016/0092-8674(90)90338-f.Peer-Reviewed Original ResearchAnimalsBase SequenceBlotting, NorthernCarcinoma, Ehrlich TumorCell NucleolusDNA, RibosomalEndoribonucleasesMiceMolecular Sequence DataNucleic Acid ConformationOligonucleotide ProbesRibonuclease HRibonucleoproteinsRibonucleoproteins, Small NuclearRNA PrecursorsRNA Processing, Post-TranscriptionalRNA, Small NuclearTemplates, Genetic
1988
Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc.
Montzka KA, Steitz JA. Additional low-abundance human small nuclear ribonucleoproteins: U11, U12, etc. Proceedings Of The National Academy Of Sciences Of The United States Of America 1988, 85: 8885-8889. PMID: 2973606, PMCID: PMC282611, DOI: 10.1073/pnas.85.23.8885.Peer-Reviewed Original ResearchConceptsSmall nuclear ribonucleoproteinU12 small nuclear ribonucleoproteinsSm small nuclear ribonucleoproteinsTrimethylguanosine cap structureTwo-dimensional gel fractionationLow-abundance RNAsLower eukaryotesSmall RNAsMRNA processingMammalian cellsMRNA splicingNuclear ribonucleoproteinAdditional speciesCap structureNuclease sensitivityNew RNASm classSm epitopesRNAU11Gel fractionationPotential roleU12EukaryotesSplicing