2001
Proximity of the invariant loop of U5 snRNA to the second intron residue during pre‐mRNA splicing
McConnell T, Steitz J. Proximity of the invariant loop of U5 snRNA to the second intron residue during pre‐mRNA splicing. The EMBO Journal 2001, 20: 3577-3586. PMID: 11432844, PMCID: PMC125517, DOI: 10.1093/emboj/20.13.3577.Peer-Reviewed Original ResearchAnimalsAzidesBase SequenceCross-Linking ReagentsEnhancer Elements, GeneticGlobinsIntronsKineticsMammalsModels, MolecularMolecular Sequence DataNucleic Acid ConformationPlasmidsPolymerase Chain ReactionRibonuclease HRibonucleoproteins, Small NuclearRNA PrecursorsRNA SplicingRNA, Small NuclearThionucleotides
1997
A new strategy for introducing photoactivatable 4-thiouridine ((4S)U) into specific positions in a long RNA molecule.
Yu YT, Steitz JA. A new strategy for introducing photoactivatable 4-thiouridine ((4S)U) into specific positions in a long RNA molecule. RNA 1997, 3: 807-10. PMID: 9214662, PMCID: PMC1369526.Peer-Reviewed Original ResearchMeSH KeywordsAffinity LabelsBiochemistryDNA-Directed RNA PolymerasesPolymerase Chain ReactionRibonuclease HRNAThiouridineTranscription, GeneticConceptsPre-mRNAPre-mRNA substrateAT-AC intronsPhage RNA polymeraseRNA-DNA chimerasFull-length RNALong RNA moleculesRNA polymeraseRNA moleculesT4 RNA ligaseT4 DNA ligaseRNA ligaseDNA ligaseRNARNase H cleavageLigaseSpecific sitesSpecific positionsIntronsPolymeraseChimerasNew strategyCleavageOligonucleotideH cleavageSite-specific crosslinking of mammalian U11 and U6atac to the 5′ splice site of an AT–AC intron
Yu Y, Steitz J. Site-specific crosslinking of mammalian U11 and U6atac to the 5′ splice site of an AT–AC intron. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 6030-6035. PMID: 9177163, PMCID: PMC20995, DOI: 10.1073/pnas.94.12.6030.Peer-Reviewed Original Research
1996
A Novel Spliceosome Containing U11, U12, and U5 snRNPs Excises a Minor Class (AT–AC) Intron In Vitro
Tarn W, Steitz J. A Novel Spliceosome Containing U11, U12, and U5 snRNPs Excises a Minor Class (AT–AC) Intron In Vitro. Cell 1996, 84: 801-811. PMID: 8625417, DOI: 10.1016/s0092-8674(00)81057-0.Peer-Reviewed Original ResearchMeSH KeywordsBase CompositionBase SequenceBlotting, NorthernCell NucleusHeLa CellsHumansMolecular Sequence DataNucleic Acid ConformationOligodeoxyribonucleotidesPlasmidsPolymerase Chain ReactionRibonuclease HRibonucleoprotein, U5 Small NuclearRibonucleoproteins, Small NuclearRNA PrecursorsRNA SplicingConceptsU5 small nuclear ribonucleoproteinSmall nuclear ribonucleoproteinU12 small nuclear ribonucleoproteinsMinor class intronsProtein coding genesPre-mRNA substrateNative gel electrophoresisCoding genesBranch site sequenceSplicing complexesNuclear ribonucleoproteinPre-mRNAP120 geneLariat intermediateSite sequenceIntronsHeLa cellsEssential roleSplicingGel electrophoresisBranch siteGenesU12Minor classU11
1991
EAP, a highly conserved cellular protein associated with Epstein‐Barr virus small RNAs (EBERs).
Toczyski DP, Steitz JA. EAP, a highly conserved cellular protein associated with Epstein‐Barr virus small RNAs (EBERs). The EMBO Journal 1991, 10: 459-466. PMID: 1846807, PMCID: PMC452667, DOI: 10.1002/j.1460-2075.1991.tb07968.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceB-LymphocytesBase SequenceCell LineCell Transformation, ViralCloning, MolecularDNAHerpesvirus 4, HumanHumansMolecular Sequence DataPolymerase Chain ReactionProteinsRibonucleoproteinsRibonucleoproteins, Small NuclearRibosomal ProteinsRNA-Binding ProteinsRNA, Small NuclearRNA, ViralSequence Homology, Nucleic Acid