1989
[31] Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing
Parker K, Steitz J. [31] Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing. Methods In Enzymology 1989, 180: 454-468. PMID: 2482428, DOI: 10.1016/0076-6879(89)80117-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesCarrier ProteinsElectrophoresis, Polyacrylamide GelEndoribonucleasesHumansIndicators and ReagentsKineticsMiceMicrococcal NucleaseNucleotide MappingRibonuclease HRibonuclease T1RibonucleasesRibonucleoproteinsRNARNA PrecursorsRNA SplicingRNA-Binding ProteinsRNA, Ribosomal, 28STranscription, Genetic
1988
An in vitro interaction between the human U3 snRNP and 28S rRNA sequences near the α-sarcin site
Parker K, Bruzik J, Steitz J. An in vitro interaction between the human U3 snRNP and 28S rRNA sequences near the α-sarcin site. Nucleic Acids Research 1988, 16: 10493-10509. PMID: 2974535, PMCID: PMC338920, DOI: 10.1093/nar/16.22.10493.Peer-Reviewed Original ResearchAntibodiesBase CompositionBase SequenceEndoribonucleasesFungal ProteinsHeLa CellsHumansMolecular Sequence DataNucleic Acid ConformationPlasmidsProtein Synthesis InhibitorsRibonuclease T1RibonucleoproteinsRibonucleoproteins, Small NuclearRNA PrecursorsRNA, RibosomalRNA, Ribosomal, 28STranscription, Genetic
1985
U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing
Black D, Chabot B, Steitz J. U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing. Cell 1985, 42: 737-750. PMID: 2996775, DOI: 10.1016/0092-8674(85)90270-3.Peer-Reviewed Original Research
1983
The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro
Mount S, Pettersson I, Hinterberger M, Karmas A, Steitz J. The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro. Cell 1983, 33: 509-518. PMID: 6190573, DOI: 10.1016/0092-8674(83)90432-4.Peer-Reviewed Original ResearchConceptsRNA-protein complexesSplice siteU1 snRNPGlobin RNAPartial proteinase K digestionU1 snRNPsMouse beta-globin geneBacteriophage T7 RNA polymeraseEucaryotic cell nucleusMessenger RNA splicingT7 RNA polymeraseRecombinant DNA clonesBeta-globin geneT1 ribonuclease digestionSplice site sequencesSmall intronsSnRNP proteinsRNA splicingSnRNP complexDNA clonesRNA polymeraseSnRNPSite sequenceRibonuclease digestionProteinase K digestionA mammalian tRNA His-Containing antigen is recognized by the polymyosits-specific antibody antijo-1
Rosa M, Hendrick J, Lerner M, Steitz J, Reichlin M. A mammalian tRNA His-Containing antigen is recognized by the polymyosits-specific antibody antijo-1. Nucleic Acids Research 1983, 11: 853-870. PMID: 6188108, PMCID: PMC325757, DOI: 10.1093/nar/11.3.853.Peer-Reviewed Original ResearchConceptsMammalian cell cytoplasmWobble positionMammalian tRNAHuman HeLaProteinase K treatmentRNA sequencesBase queuineTerminal nucleotidesCell extractsTRNACell cytoplasmAntigenic proteinsImmunofluorescence studiesPhenol extractionK treatmentSephacryl SMethylated guaninesAntigenic formsTRNAHisAntigenic complexQueuineAnticodonUnusual featuresColumn chromatographyNucleotides
1981
Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus.
Lerner MR, Andrews NC, Miller G, Steitz JA. Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus. Proceedings Of The National Academy Of Sciences Of The United States Of America 1981, 78: 805-809. PMID: 6262773, PMCID: PMC319891, DOI: 10.1073/pnas.78.2.805.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusLupus erythematosusEBER-1EBER-2EBV DNAB lymphocytesEpstein-Barr virus genomeEpstein-Barr virusNormal B lymphocytesEcoRI J fragmentT lymphocytesErythematosusLa antigenLymphocytesPrimate cellsHerpesvirus atelesExhibit sequence homologyAntibodiesPotential relationshipVirus genomeCell transformationCellsUninfected mammalian cellsRNAMammalian cellsArrangement of 30S heterogeneous nuclear ribonucleoprotein on polyoma virus late nuclear transcripts.
Steitz JA, Kamen R. Arrangement of 30S heterogeneous nuclear ribonucleoprotein on polyoma virus late nuclear transcripts. Molecular And Cellular Biology 1981, 1: 21-34. PMID: 6100958, PMCID: PMC369323, DOI: 10.1128/mcb.1.1.21.Peer-Reviewed Original ResearchSequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing
Mount S, Steitz J. Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing. Nucleic Acids Research 1981, 9: 6351-6368. PMID: 6172778, PMCID: PMC327608, DOI: 10.1093/nar/9.23.6351.Peer-Reviewed Original ResearchConceptsU1 RNAU1 sequenceCultured Drosophila melanogaster cellsDrosophila U1 RNAU1 RNA sequenceDrosophila melanogaster cellsHuman U1 RNASecondary structure modelDNA sequence determinationDonor splice junctionGenomic clonesDrosophila melanogasterSplicing reactionRNA moleculesU1 snRNPsConsensus sequenceSplice junctionsRNA sequencesNucleotides 3Sequence determinationSecondary structureRNASplicingMolecular interactionsSequence
1979
Tandem promoters direct E. coli ribosomal RNA synthesis
Young R, Steitz J. Tandem promoters direct E. coli ribosomal RNA synthesis. Cell 1979, 17: 225-234. PMID: 110460, DOI: 10.1016/0092-8674(79)90310-6.Peer-Reviewed Original Research
1977
The nucleotide sequence of a major glycine transfer RNA from the posterior silk gland of Bombyx mori L.
Zúñiga M, Steitz J. The nucleotide sequence of a major glycine transfer RNA from the posterior silk gland of Bombyx mori L. Nucleic Acids Research 1977, 4: 4175-4196. PMID: 414206, PMCID: PMC343233, DOI: 10.1093/nar/4.12.4175.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBombyxExocrine GlandsGlycineMolecular WeightOligoribonucleotidesRibonuclease T1RNA, Transfer