Calcium, ATP and nuclear pore channel gating
Bustamante J, Michelette E, Geibel J, Dean D, Hanover J, McDonnell T. Calcium, ATP and nuclear pore channel gating. Pflügers Archiv - European Journal Of Physiology 2000, 439: 433-444. PMID: 10678739, PMCID: PMC4400178, DOI: 10.1007/s004249900189.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsAntibodies, MonoclonalBiological TransportCalciumCalcium ChannelsChelating AgentsCytosolDextransEgtazic AcidEndoplasmic ReticulumEnzyme InhibitorsFluorescein-5-isothiocyanateFluorescent DyesGene Expression Regulation, NeoplasticInositol 1,4,5-TrisphosphateIon Channel GatingMaleNuclear EnvelopeOocytesPatch-Clamp TechniquesProstatic NeoplasmsThapsigarginTumor Cells, CulturedXenopus laevisConceptsNPC channelGene activityChannel gatingMacromolecular transportCytosolic Ca2Fluorescence microscopy observationsTranscription factorsNE membranesProstate cancer cellsCytosolic ATPIntracellular messengerCellular rhythmsRole of Ca2ATPCancer cellsPatch-clamp studiesPresent patch-clamp studyRNAIon channel behaviorRegulationCardiomyocyte nucleiGatingExpressionCa2ThapsigarginDendrimer-assisted patch-clamp sizing of nuclear pores
Bustamante J, Michelette E, Geibel J, Hanover J, McDonnell T, Dean D. Dendrimer-assisted patch-clamp sizing of nuclear pores. Pflügers Archiv - European Journal Of Physiology 2000, 439: 829-837. PMID: 10784359, PMCID: PMC4397974, DOI: 10.1007/s004249900233.Peer-Reviewed Original ResearchConceptsNuclear pore complexNuclear localization signalMacromolecular translocationFluorescence microscopy dataNuclear envelopeGreen fluorescence proteinLocalization signalPore complexNuclear poresMicroscopy dataColloidal gold particlesFluorescence proteinElectron microscopyCell lysatesFluorescence microscopyPhysiological conditionsDistinct classesCellular structureGold particlesProteinTranslocationUseful probeCardiomyocyte nucleiB-phycoerythrinParticles