2022
Colonic Fluid and Electrolyte Transport 2022: An Update
Negussie AB, Dell AC, Davis BA, Geibel JP. Colonic Fluid and Electrolyte Transport 2022: An Update. Cells 2022, 11: 1712. PMID: 35626748, PMCID: PMC9139964, DOI: 10.3390/cells11101712.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane regulatorCell death regulationSignal transduction pathwaysDeath regulationNa-K-Cl cotransporterEpithelial cell deathTransduction pathwaysIon homeostasisRegulatory relationshipsIon transportersTransepithelial ion transportEpithelial sodium channelTransmembrane regulatorCell deathColonic epithelial cellsEpithelial cellsTransportersSodium channelsNa-H exchangerRegulationIon transportCancer pathologyPH modulationIon movementOsmotic gradient
2013
Identification of Functionally Distinct Na-HCO3 Co-Transporters in Colon
Barmeyer C, Ye JH, Soroka C, Geibel P, Hingsammer LM, Weitgasser L, Atway D, Geibel JP, Binder HJ, Rajendran VM. Identification of Functionally Distinct Na-HCO3 Co-Transporters in Colon. PLOS ONE 2013, 8: e62864. PMID: 23690961, PMCID: PMC3653958, DOI: 10.1371/journal.pone.0062864.Peer-Reviewed Original ResearchConceptsNa-HCO3 cotransportDistal colonProximal colonNBC activityNBC isoformsRat colonColonic epithelial cellsCrypt glandsSensitive transporterHCO3- secretionColonHCO3-dependent mechanismEpithelial cellsSaturable processHOE694Isoform 1Basolateral membraneCotransportPCR analysisNorthern blotDIDSMolecular studiesMolecular identityApparent KiCritical role
2004
Extracellular polyamines regulate fluid secretion in rat colonic crypts via the extracellular calcium-sensing receptor
Cheng SX, Geibel JP, Hebert SC. Extracellular polyamines regulate fluid secretion in rat colonic crypts via the extracellular calcium-sensing receptor. Gastroenterology 2004, 126: 148-158. PMID: 14699496, DOI: 10.1053/j.gastro.2003.10.064.Peer-Reviewed Original ResearchConceptsCalcium-sensing receptorColonic epithelial cellsFluid secretionColonic cryptsCalcium-sensing receptor activationExtracellular calcium-sensing receptorEpithelial cellsIntracellular D-myo-inositolNormal postnatal developmentRat colonic cryptsExtracellular polyaminesDistal colonDistal colonic cryptsSecond messenger generationDistal colonic epithelial cellsGastrointestinal epitheliumSecond messenger accumulationPostnatal developmentReceptor activationExtracellular spermineIntact cryptsReceptorsColonocyte functionSecretionCrypts
2003
Characterization of Cl-HCO3 exchange in basolateral membrane of rat distal colon
Ikuma M, Geibel J, Binder HJ, Rajendran VM. Characterization of Cl-HCO3 exchange in basolateral membrane of rat distal colon. American Journal Of Physiology - Cell Physiology 2003, 285: c912-c921. PMID: 12826602, DOI: 10.1152/ajpcell.00396.2002.Peer-Reviewed Original ResearchConceptsRat distal colonCl-HCO3 exchangeDistal colonBasolateral membraneHalf-maximal inhibitory constantsBasolateral membrane vesiclesCl- channel blockerColonic epithelial cellsAbsence of HCO3Channel blockersRat colonic epithelial cellsCrypt preparationCrypt cellsExchange inhibitorCl-OH exchangeRemoval of ClColonEpithelial cellsAnion exchange inhibitorHCO3- gradientProtease inhibitorsK ionophorePresent studyInhibitory constantsProtease inhibitor cocktailNa-H Exchange Function in Colonic Epithelial Cells
Binder H, Rajendran V, Geibel J. Na-H Exchange Function in Colonic Epithelial Cells. 2003, 71-90. DOI: 10.1007/978-1-4615-0427-6_5.Peer-Reviewed Original ResearchColonic epithelial cellsShort-chain fatty acidsEpithelial cellsSmall intestinal epithelial cellsAbsorption of carbohydratesIntestinal epithelial cellsSecretion of fluidEpithelial cell functionNeonatal periodCrypt cellsDistal segmentsCell functionSignificant heterogeneityMammalian colonSecretory processColonFatty acidsCl transportMultiple factorsCellsNutrient absorptionExchange functionHormoneSecretion
2001
Cloning and Expression of a Chloride-dependent Na+-H+ Exchanger*
Sangan P, Rajendran VM, Geibel JP, Binder HJ. Cloning and Expression of a Chloride-dependent Na+-H+ Exchanger*. Journal Of Biological Chemistry 2001, 277: 9668-9675. PMID: 11773056, DOI: 10.1074/jbc.m110852200.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAmino Acid SequenceAnimalsBlotting, NorthernCell LineChlorineCloning, MolecularColonDiureticsDNA, ComplementaryFibroblastsHydrogen-Ion ConcentrationIn Situ HybridizationMaleMolecular Sequence DataPhosphorylationProtein Structure, TertiaryRatsRats, Sprague-DawleyRNA, MessengerSequence Homology, Amino AcidSodiumSodium-Hydrogen Exchanger 1Sodium-Hydrogen ExchangersTime FactorsTissue DistributionConceptsRat distal colonDistal colonAmino acidsCrypt cellsExchange isoformsDietary sodium depletionColonic crypt cellsFull-length cDNACl- channel blockerColonic epithelial cellsN-terminal regionNorthern blot analysisSodium depletionChannel blockersNovel C-terminusPutative transmembranePS120 fibroblastsAcid loadDependent intracellularC-terminusNHE-1Epithelial cellsIsoform inhibitorAmiloride analoguesMultiple tissuesCharacterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon
Rajendran V, Geibel J, Binder H. Characterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon. AJP Gastrointestinal And Liver Physiology 2001, 280: g400-g405. PMID: 11171622, DOI: 10.1152/ajpgi.2001.280.3.g400.Peer-Reviewed Original ResearchConceptsRat distal colonNa/H exchangeCl- channel blockerDistal colonChannel blockersCrypt cellsIntracellular pH recoveryExchange inhibitor amilorideColonic epithelial cellsApical membrane vesiclesCrypt glandsHigh doseHOE 694NA depletionAcid loadExchange inhibitorInhibitor amiloridePH recoveryEpithelial cellsUptake 1NHE isoformsAnion exchange inhibitorBlockersColonNa uptake