2012
Effect of the AMP-Kinase Modulators AICAR, Metformin and Compound C on Insulin Secretion of INS-1E Rat Insulinoma Cells under Standard Cell Culture Conditions
Langelueddecke C, Jakab M, Ketterl N, Lehner L, Hufnagl C, Schmidt S, Geibel JP, Fuerst J, Ritter M. Effect of the AMP-Kinase Modulators AICAR, Metformin and Compound C on Insulin Secretion of INS-1E Rat Insulinoma Cells under Standard Cell Culture Conditions. Cellular Physiology And Biochemistry 2012, 29: 75-86. PMID: 22415077, DOI: 10.1159/000337589.Peer-Reviewed Original ResearchConceptsAMP-dependent protein kinaseCompound CStandard cell culture conditionsCell culture conditionsINS-1E rat insulinoma cellsAMPK phosphorylationAMPK inhibitor compound CCulture conditionsCell membrane potentialAMPK-independent mechanismCell metabolic stateInhibitor compound CINS-1E cellsMetabolic stateMembrane potentialStimulus-secretion couplingBasal AMPK phosphorylationΒ-cell survivalCell cycle arrestProtein kinaseΒ-cell line INS-1E.Nutrient uptakeAMPK activatorCell cycle distributionInsulin secretion
2010
Ethanol Inhibits Gastric Acid Secretion in Rats Through Increased AMP-Kinase Activity
Kopic S, Corradini S, Sidani S, Murek M, Vardanyan A, Föller M, Ritter M, Geibel JP. Ethanol Inhibits Gastric Acid Secretion in Rats Through Increased AMP-Kinase Activity. Cellular Physiology And Biochemistry 2010, 25: 195-202. PMID: 20110680, DOI: 10.1159/000276553.Peer-Reviewed Original ResearchConceptsGastric acid secretionAcid secretionSecretagogue-induced acid secretionInhibits gastric acid secretionShort-term ethanol exposurePH-sensitive dye BCECFLow-dose ethanolCompound CAMPK inhibitor compound CSpecific AMPK inhibitor compound CEffects of ethanolInhibitor compound CEthanol exposureRat gastric glandsActivation of AMPKInteraction of ethanolRat gastric parietal cellsFluorescence digital imagingGastric parietal cellsAMP kinase activityDye BCECFInhibitory effectSecretionParietal cellsAMPK pathway
2009
AMP-activated protein kinase: a physiological off switch for murine gastric acid secretion
Sidani S, Kopic S, Socrates T, Kirchhoff P, Föller M, Murek M, Capasso A, Geibel JP. AMP-activated protein kinase: a physiological off switch for murine gastric acid secretion. Pflügers Archiv - European Journal Of Physiology 2009, 459: 39. PMID: 19621238, DOI: 10.1007/s00424-009-0698-3.Peer-Reviewed Original ResearchConceptsProtein kinaseAMPK activationSecretagogue-induced acid secretionCellular ATP levelsPresence of AMPKEnergy-dependent transportersImmunofluorescent localizationParietal cellsGastric glandsCellular ATP consumptionGastric parietal cellsCompound CAMPKProton effluxATP consumptionMetabolic stressRate of intracellularATP levelsKinaseAdenosine monophosphateRegulatorATPLarge abundanceCellsActivationThe Phytostilbene Resveratrol Induces Apoptosis in INS-1E Rat Insulinoma Cells
Bortolotti C, Kunit T, Moder A, Hufnagl C, Schmidt S, Hartl A, Langelueddecke C, Fürst J, Geibel J, Ritter M, Jakab M. The Phytostilbene Resveratrol Induces Apoptosis in INS-1E Rat Insulinoma Cells. Cellular Physiology And Biochemistry 2009, 23: 245-254. PMID: 19471092, DOI: 10.1159/000218171.Peer-Reviewed Original ResearchConceptsINS-1E rat insulinoma cellsINS-1E cellsApoptotic volume decreaseRat insulinoma cellsAkt phosphorylationInsulinoma cellsCell proliferationProapoptotic effectsInhibitor compound CBasal Akt phosphorylationExposure of phosphatidylserineInduction of apoptosisG0/G1 phaseCaspase activationPlasma membraneOuter leafletCompound CMuM resveratrolG1 phaseCrystal violet stainingApoptotic cellsCell granularityAddition of insulinPhosphorylationMajor target
2007
Ethanol inhibits gastric acid secretion in rats through increased AMP‐K activity
Corradini S, Kopic S, Ritter M, Geibel J. Ethanol inhibits gastric acid secretion in rats through increased AMP‐K activity. The FASEB Journal 2007, 21: a1317-a1317. DOI: 10.1096/fasebj.21.6.a1317-c.Peer-Reviewed Original ResearchSecretagogue-induced acid secretionAcid secretionRate of alkalinizationLow-dose ethanol exposureCompound CPH-sensitive dye BCECFLow-dose ethanolGastric acid secretionInhibitor compound CEthanol exposureRat gastric glandsVariety of cellsNH4Cl prepulseRat gastric parietal cellsGastric parietal cellsSecretagogue carbacholPotent modulatorDye BCECFInhibitory effectParietal cellsSecretionGastric glandsAMP kinasePresent studyGland