2005
Insulin Receptor (IR) and Glucose Transporter 2 (GLUT2) Proteins Form a Complex on the Rat Hepatocyte Membrane
Eisenberg M, Maker A, Slezak L, Nathan J, Sritharan K, Jena B, Geibel J, Andersen D. Insulin Receptor (IR) and Glucose Transporter 2 (GLUT2) Proteins Form a Complex on the Rat Hepatocyte Membrane. Cellular Physiology And Biochemistry 2005, 15: 051-058. PMID: 15665515, DOI: 10.1159/000083638.Peer-Reviewed Original ResearchConceptsGlucose transporter 2Insulin receptorPlasma membranePlasma membrane insulin receptorsFluorescence resonance energy transfer studiesMembrane insulin receptorsHepatic glucose transportersGlucose transporterHepatocyte plasma membraneHepatic glucose regulationInsulin regulationGlucose transportBidirectional glucose transportRat hepatocyte membranesConfocal microscopyTransporter 2MembraneEnergy transfer studiesRegulationTexas RedComplexesEndosomesCytosolProteinTransporters
2001
K+-induced HSP-72 expression is mediated via rapid Ca2+ influx in renal epithelial cells
Eickelberg O, Geibel J, Seebach F, Giebisch G, Kashgarian M. K+-induced HSP-72 expression is mediated via rapid Ca2+ influx in renal epithelial cells. American Journal Of Physiology. Renal Physiology 2001, 281: f280-f287. PMID: 11457719, DOI: 10.1152/ajprenal.2001.281.2.f280.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium Channel BlockersCell LineDiltiazemEgtazic AcidEnzyme InhibitorsEpithelial CellsGallic AcidGenes, ReporterHeat-Shock ProteinsHSP72 Heat-Shock ProteinsImmunoblottingKidney Tubules, ProximalMicroscopy, ConfocalPotassiumPromoter Regions, GeneticRecombinant Fusion ProteinsSodiumSwineThapsigarginUrotheliumConceptsHSP 72 expressionPromoter activityHSP 72Protein expressionProtective cellular responseLuciferase reporter geneHSP-25Heat shock protein expressionRenal epithelial cellsTranscriptional inductionShock protein expressionIonic stressReporter geneHSP-90 levelsHSC 73Cellular responsesChannel blocker diltiazemIntracellular lumenWestern blot analysisChelator EGTA-AMPathophysiological stimuliBlot analysisConfocal microscopyProtein levelsExtracellular space
2000
Cell pH and H+ Secretion by S3 Segment of Mammalian Kidney: Role of H+-ATPase and Cl−
Malnic G, Geibel J. Cell pH and H+ Secretion by S3 Segment of Mammalian Kidney: Role of H+-ATPase and Cl−. The Journal Of Membrane Biology 2000, 178: 115-125. PMID: 11083900, DOI: 10.1007/s002320010019.Peer-Reviewed Original ResearchConceptsProximal tubule cellsChannel blocker NPPBS3 segmentTubule cellsProximal S3 segmentsFree mediumCell pHConfocal microscopyNIH Image programCell pH regulationMicroperfusion techniqueSuperfusionMouse kidneyRabbit kidneyKidneySubcellular vesiclesApical areaCell acidificationMammalian kidneyFluorescence densityNPPBRingerCellsImage programNaCl Ringer
1997
Gi regulation of secretory vesicle swelling examined by atomic force microscopy
Jena B, Schneider S, Geibel J, Webster P, Oberleithner H, Sritharan K. Gi regulation of secretory vesicle swelling examined by atomic force microscopy. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 13317-13322. PMID: 9371843, PMCID: PMC24306, DOI: 10.1073/pnas.94.24.13317.Peer-Reviewed Original ResearchConceptsSecretory vesicle membraneVesicle swellingSecretory vesiclesPlasma membraneVesicle fusionVesicle membraneSecretory vesicle swellingSecretory vesicle fusionConfocal microscopyZymogen granulesCell plasma membraneVesicle volumeHeterotrimeric guanineHeterotrimeric GTPZymogen granule sizeGTPase activityAlpha subunitMolecular mechanismsRegulatory rolePresence of GTPExocytotic processMastoparan analogsProteinGi proteinsMas7
1994
Characterization of Parietal Cell Functional Morphology Utilizing Isolated Gastric Gland Perfusion, Atomic Force and Confocal Microscopy
Modlin I, Waisbren S, Boron W, Soroka C, Goldenring J, Geibel J. Characterization of Parietal Cell Functional Morphology Utilizing Isolated Gastric Gland Perfusion, Atomic Force and Confocal Microscopy. NATO ASI Series 1994, 329-339. DOI: 10.1007/978-3-642-79301-1_38.Peer-Reviewed Original ResearchMembrane functionMembrane movementFunctional morphologyCell functionApical membrane surfaceConfocal fluorescence microscopyParietal cellsCytoskeletal transformationProton pumpGastric gland preparationProteolytic contentCell culture systemParietal cell functionFluorescence microscopyConfocal microscopySecretory processGland preparationsCulture systemCellsGastric glandsParietal cell preparationsCell preparationsMembrane surfaceLow pH