2022
Colonic Fluid and Electrolyte Transport 2022: An Update
Negussie AB, Dell AC, Davis BA, Geibel JP. Colonic Fluid and Electrolyte Transport 2022: An Update. Cells 2022, 11: 1712. PMID: 35626748, PMCID: PMC9139964, DOI: 10.3390/cells11101712.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane regulatorCell death regulationSignal transduction pathwaysDeath regulationNa-K-Cl cotransporterEpithelial cell deathTransduction pathwaysIon homeostasisRegulatory relationshipsIon transportersTransepithelial ion transportEpithelial sodium channelTransmembrane regulatorCell deathColonic epithelial cellsEpithelial cellsTransportersSodium channelsNa-H exchangerRegulationIon transportCancer pathologyPH modulationIon movementOsmotic gradient
2013
Identification of Functionally Distinct Na-HCO3 Co-Transporters in Colon
Barmeyer C, Ye JH, Soroka C, Geibel P, Hingsammer LM, Weitgasser L, Atway D, Geibel JP, Binder HJ, Rajendran VM. Identification of Functionally Distinct Na-HCO3 Co-Transporters in Colon. PLOS ONE 2013, 8: e62864. PMID: 23690961, PMCID: PMC3653958, DOI: 10.1371/journal.pone.0062864.Peer-Reviewed Original ResearchConceptsNa-HCO3 cotransportDistal colonProximal colonNBC activityNBC isoformsRat colonColonic epithelial cellsCrypt glandsSensitive transporterHCO3- secretionColonHCO3-dependent mechanismEpithelial cellsSaturable processHOE694Isoform 1Basolateral membraneCotransportPCR analysisNorthern blotDIDSMolecular studiesMolecular identityApparent KiCritical role
2009
The Functions and Roles of the Extracellular Ca2+–Sensing Receptor along the Gastrointestinal Tract
Geibel JP, Hebert SC. The Functions and Roles of the Extracellular Ca2+–Sensing Receptor along the Gastrointestinal Tract. Annual Review Of Physiology 2009, 71: 205-217. PMID: 19575679, DOI: 10.1146/annurev.physiol.010908.163128.Peer-Reviewed Original ResearchConceptsGastrointestinal tractNormal gut physiologyNutrient availabilityDigestion of foodRapid turnoverAmino acidsColonic fluid transportFunctional digestive tractGastric acid secretionCalcium-sensing receptorOrganic nutrientsWater homeostasisEntire gastrointestinal tractPotent targetGut physiologyEpithelial cellsIntestinal epitheliumSecretory diarrheaAcid secretionDigestive tractExtracellular Ca2Colon cancerReceptorsNormal saltFluid secretion
2006
Calcium-sensing receptor abrogates secretagogue- induced increases in intestinal net fluid secretion by enhancing cyclic nucleotide destruction
Geibel J, Sritharan K, Geibel R, Geibel P, Persing JS, Seeger A, Roepke TK, Deichstetter M, Prinz C, Cheng SX, Martin D, Hebert SC. Calcium-sensing receptor abrogates secretagogue- induced increases in intestinal net fluid secretion by enhancing cyclic nucleotide destruction. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 9390-9397. PMID: 16760252, PMCID: PMC1475505, DOI: 10.1073/pnas.0602996103.Peer-Reviewed Original ResearchConceptsCalcium-sensing receptorCholera toxinFluid secretionNet fluid secretionSecretagogue-induced increasesIntestinal fluid transportCaSR agonistsSecretagogue actionUnique therapyCaSR signalingSecretory actionSecretory diarrheaReceptor activationIntestinal tractEpithelial cellsEnterotoxin STaReceptorsCyclic nucleotidesSecretionExtracellular milieuDiverse cellsToxinActivationCellsDiarrheaRole of calcium and other trace elements in the gastrointestinal physiology
Kirchhoff P, Geibel J. Role of calcium and other trace elements in the gastrointestinal physiology. World Journal Of Gastroenterology 2006, 12: 3229-3236. PMID: 16718844, PMCID: PMC4087967, DOI: 10.3748/wjg.v12.i20.3229.Peer-Reviewed Original ResearchConceptsCell type changesGastrointestinal tractTerrestrial organismsNutrient availabilityIntestinal epithelial cellsCellular behaviorTerminal differentiationExact functionEssential ionsAmino acidsNeuronal functionGastric acid secretionEpithelial cellsEntire gastrointestinal tractAllosteric modifierCaSR expressionRole of calciumElevated extracellularSecretory diarrheaAcid secretionCalcium handlingReceptorsNeoplastic diseaseGastrointestinal physiologyPhysiological effects
2005
Role of short-chain fatty acids in colonic HCO3 secretion
Vidyasagar S, Barmeyer C, Geibel J, Binder HJ, Rajendran VM. Role of short-chain fatty acids in colonic HCO3 secretion. AJP Gastrointestinal And Liver Physiology 2005, 288: g1217-g1226. PMID: 15677553, DOI: 10.1152/ajpgi.00415.2004.Peer-Reviewed Original ResearchConceptsShort-chain fatty acidsSCFA/SCFA transportApical membrane vesiclesMM butyrateSurface epithelial cellsFatty acidsSCFA metabolismColonic mucosaHCO3- secretionCrypt cellsCarrier-mediated processLucite chamberSecretionColonic cryptsEpithelial cellsComparable degreeButyrate stimulationChannel activityButyrate uptakeStat techniqueCryptsAnion channel activityDIDSButyrate
2004
Functions and roles of the extracellular Ca2+-sensing receptor in the gastrointestinal tract
Hebert SC, Cheng S, Geibel J. Functions and roles of the extracellular Ca2+-sensing receptor in the gastrointestinal tract. Cell Calcium 2004, 35: 239-247. PMID: 15200147, DOI: 10.1016/j.ceca.2003.10.015.Peer-Reviewed Original ResearchConceptsNormal cell proliferationGastrointestinal tractEpithelial cellsNutrient availabilityIntestinal epithelial cellsPotential roleGastrointestinal epithelial cellsL-amino acidsFood digestionRapid turnoverNutrient absorptionCell proliferationWater homeostasisGastric acid secretionGastrointestinal biologyEntire gastrointestinal tractAllosteric modifierCalcium intakeSecretory diarrheaAcid secretionCalcium handlingExtracellular Ca2Colon cancerReceptorsPhysiological effectsExtracellular polyamines regulate fluid secretion in rat colonic crypts via the extracellular calcium-sensing receptor
Cheng SX, Geibel JP, Hebert SC. Extracellular polyamines regulate fluid secretion in rat colonic crypts via the extracellular calcium-sensing receptor. Gastroenterology 2004, 126: 148-158. PMID: 14699496, DOI: 10.1053/j.gastro.2003.10.064.Peer-Reviewed Original ResearchConceptsCalcium-sensing receptorColonic epithelial cellsFluid secretionColonic cryptsCalcium-sensing receptor activationExtracellular calcium-sensing receptorEpithelial cellsIntracellular D-myo-inositolNormal postnatal developmentRat colonic cryptsExtracellular polyaminesDistal colonDistal colonic cryptsSecond messenger generationDistal colonic epithelial cellsGastrointestinal epitheliumSecond messenger accumulationPostnatal developmentReceptor activationExtracellular spermineIntact cryptsReceptorsColonocyte functionSecretionCrypts
2003
Characterization of Cl-HCO3 exchange in basolateral membrane of rat distal colon
Ikuma M, Geibel J, Binder HJ, Rajendran VM. Characterization of Cl-HCO3 exchange in basolateral membrane of rat distal colon. American Journal Of Physiology - Cell Physiology 2003, 285: c912-c921. PMID: 12826602, DOI: 10.1152/ajpcell.00396.2002.Peer-Reviewed Original ResearchConceptsRat distal colonCl-HCO3 exchangeDistal colonBasolateral membraneHalf-maximal inhibitory constantsBasolateral membrane vesiclesCl- channel blockerColonic epithelial cellsAbsence of HCO3Channel blockersRat colonic epithelial cellsCrypt preparationCrypt cellsExchange inhibitorCl-OH exchangeRemoval of ClColonEpithelial cellsAnion exchange inhibitorHCO3- gradientProtease inhibitorsK ionophorePresent studyInhibitory constantsProtease inhibitor cocktailNa-H Exchange Function in Colonic Epithelial Cells
Binder H, Rajendran V, Geibel J. Na-H Exchange Function in Colonic Epithelial Cells. 2003, 71-90. DOI: 10.1007/978-1-4615-0427-6_5.Peer-Reviewed Original ResearchColonic epithelial cellsShort-chain fatty acidsEpithelial cellsSmall intestinal epithelial cellsAbsorption of carbohydratesIntestinal epithelial cellsSecretion of fluidEpithelial cell functionNeonatal periodCrypt cellsDistal segmentsCell functionSignificant heterogeneityMammalian colonSecretory processColonFatty acidsCl transportMultiple factorsCellsNutrient absorptionExchange functionHormoneSecretion
2002
Calcium-pump inhibitors induce functional surface expression of ΔF508-CFTR protein in cystic fibrosis epithelial cells
Egan ME, Glöckner-Pagel J, Ambrose C, Cahill PA, Pappoe L, Balamuth N, Cho E, Canny S, Wagner CA, Geibel J, Caplan MJ. Calcium-pump inhibitors induce functional surface expression of ΔF508-CFTR protein in cystic fibrosis epithelial cells. Nature Medicine 2002, 8: 485-492. PMID: 11984593, DOI: 10.1038/nm0502-485.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumCalcium pump inhibitorΔF508-CFTR proteinCystic fibrosis epithelial cellsCystic fibrosis transmembrane conductance regulator (CFTR) proteinCystic fibrosis cell lineFunctional surface expressionSurface expressionChaperone activityChaperone proteinsRegulator proteinPlasma membraneCystic fibrosis defectCell surfaceProteinCell linesPotential targetOptimal activityInhibitor thapsigarginEpithelial cellsExpressionCommon mutationsInhibitorsMouse modelReticulum
2001
Cloning and Expression of a Chloride-dependent Na+-H+ Exchanger*
Sangan P, Rajendran VM, Geibel JP, Binder HJ. Cloning and Expression of a Chloride-dependent Na+-H+ Exchanger*. Journal Of Biological Chemistry 2001, 277: 9668-9675. PMID: 11773056, DOI: 10.1074/jbc.m110852200.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAmino Acid SequenceAnimalsBlotting, NorthernCell LineChlorineCloning, MolecularColonDiureticsDNA, ComplementaryFibroblastsHydrogen-Ion ConcentrationIn Situ HybridizationMaleMolecular Sequence DataPhosphorylationProtein Structure, TertiaryRatsRats, Sprague-DawleyRNA, MessengerSequence Homology, Amino AcidSodiumSodium-Hydrogen Exchanger 1Sodium-Hydrogen ExchangersTime FactorsTissue DistributionConceptsRat distal colonDistal colonAmino acidsCrypt cellsExchange isoformsDietary sodium depletionColonic crypt cellsFull-length cDNACl- channel blockerColonic epithelial cellsN-terminal regionNorthern blot analysisSodium depletionChannel blockersNovel C-terminusPutative transmembranePS120 fibroblastsAcid loadDependent intracellularC-terminusNHE-1Epithelial cellsIsoform inhibitorAmiloride analoguesMultiple tissuesEffects of the Serine/Threonine Kinase SGK1 on the Epithelial Na+ Channel (ENaC) and CFTR: Implications for Cystic Fibrosis
Wagner C, Ott M, Klingel K, Beck S, Melzig J, Friedrich B, Wild K, Bröer S, Moschen I, Albers A, Waldegger S, Tümmler B, Egan M, Geibel J, Kandolf R, Lang F. Effects of the Serine/Threonine Kinase SGK1 on the Epithelial Na+ Channel (ENaC) and CFTR: Implications for Cystic Fibrosis. Cellular Physiology And Biochemistry 2001, 11: 209-218. PMID: 11509829, DOI: 10.1159/000051935.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAmino Acid SubstitutionAnimalsBronchiCell LineCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorEpithelial CellsEpithelial Sodium ChannelsHumansIn Situ HybridizationLungMacrophages, AlveolarMutationOocytesPatch-Clamp TechniquesProtein Serine-Threonine KinasesPulmonary AlveoliRNA, ComplementaryRNA, MessengerSodiumSodium ChannelsXenopus laevisConceptsSerine/threonine kinase SGK1Lung tissueCystic fibrosisCF patientsKinase SGK1CF lung tissueXenopus oocytesLoss of CFTRLung epithelial cell lineCoexpression of CFTREffect of SGK1Pathophysiological factorsEpithelial cell lineRespiratory epitheliumLung phenotypeVariety of stimuliCl(-) secretionSGK1 expressionInhibitor amilorideInhibitory effectEpithelial cellsEnhanced expressionChannel ENaC.CFTR mutationsChannel activityCharacterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon
Rajendran V, Geibel J, Binder H. Characterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon. AJP Gastrointestinal And Liver Physiology 2001, 280: g400-g405. PMID: 11171622, DOI: 10.1152/ajpgi.2001.280.3.g400.Peer-Reviewed Original ResearchConceptsRat distal colonNa/H exchangeCl- channel blockerDistal colonChannel blockersCrypt cellsIntracellular pH recoveryExchange inhibitor amilorideColonic epithelial cellsApical membrane vesiclesCrypt glandsHigh doseHOE 694NA depletionAcid loadExchange inhibitorInhibitor amiloridePH recoveryEpithelial cellsUptake 1NHE isoformsAnion exchange inhibitorBlockersColonNa uptake
1999
Expression of an extracellular calcium-sensing receptor in rat stomach
Cheng* ‡, Qureshi§ I, Chattopadhyay‡ N, Qureshi§ A, Butters‡ R, Hall∥ A, Cima* R, Rogers∥ K, Hebert∥ S, Geibel§ J, Brown‡ E, Soybel* D, ¶NPS Pharmaceuticals I. Expression of an extracellular calcium-sensing receptor in rat stomach. Gastroenterology 1999, 116: 118-126. PMID: 9869609, DOI: 10.1016/s0016-5085(99)70235-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, NorthernCalciumCells, CulturedDNA, ComplementaryExtracellular SpaceGastric MucosaImmunohistochemistryMolecular Sequence DataMuscle, SmoothParietal Cells, GastricRatsRats, Sprague-DawleyReceptors, Calcium-SensingReceptors, Cell SurfaceReverse Transcriptase Polymerase Chain ReactionRNAStomachTranscription, GeneticConceptsGastric glandsExtracellular calcium-sensing receptorEpithelial cellsCalcium-sensing receptorReverse transcription-polymerase chain reactionGastric surface cellsMammalian gastric mucosaExpression of CARMyenteric neuronsSubmucosal plexusSurface cellsGastric functionPolymerase chain reactionNerve regionRat stomachGastric mucosaGlandular stomachExtracellular Ca2Secretory functionRat parathyroidSystemic Ca2AbstractTextLevels of Ca2CAR expressionStomach
1998
Sorting of P-type ATPases in polarized epithelial cells.
Dunbar LA, Courtois-Coutry N, Roush DL, Muth TR, Gottardi CJ, Rajendran V, Geibel J, Kashgarian M, Caplan MJ. Sorting of P-type ATPases in polarized epithelial cells. Acta Physiologica Scandinavica. Supplementum 1998, 643: 289-95. PMID: 9789572.Peer-Reviewed Original ResearchConceptsApical localizationK-ATPaseMost epithelial cell typesTyrosine-based signalsP-type familyP-type ATPasesEpithelial cellsCritical tyrosine residuesApical plasma membraneFourth transmembrane domainBeta-subunit sequencesApical cell surfaceEpithelial cell typesSorting signalsTransmembrane domainCytoplasmic tailSequence domainsPlasma membraneHomologous membersTyrosine residuesParietal cellsStorage compartmentCell typesCell surfaceBasolateral surfaceDifferential localization of colonic H+-K+-ATPase isoforms in surface and crypt cells
Rajendran V, Singh S, Geibel J, Binder H. Differential localization of colonic H+-K+-ATPase isoforms in surface and crypt cells. American Journal Of Physiology 1998, 274: g424-g429. PMID: 9486199, DOI: 10.1152/ajpgi.1998.274.2.g424.Peer-Reviewed Original ResearchConceptsCrypt cellsColonic cryptsRat distal colonSurface cellsSurface epithelial cellsATPase isoformsOuabain-insensitive componentApical membraneDistal colonAcid loadDependent intracellularEpithelial cellsATPase activityCryptsDifferential localizationOuabainATPase alpha subunitCellsAlpha subunitCompelling evidenceIsoformsColonActivityRecovery
1997
Life on biomembranes viewed with the atomic force microscope.
Oberleithner H, Geibel J, Guggino W, Henderson R, Hunter M, Schneider S, Schwab A, Wang W. Life on biomembranes viewed with the atomic force microscope. Wiener Klinische Wochenschrift 1997, 109: 419-23. PMID: 9261980.Peer-Reviewed Original ResearchConceptsAtomic force microscopeForce microscopeNear-field microscopePlasma membrane turnoverSoft biological surfacesPore complexRenal epithelial cellsAtomic resolutionNuclear poresXenopus laevis oocytesNuclear envelopeAFM techniquesExperimental biologistsLaevis oocytesMembrane turnoverKidney cellsEpithelial cellsMicroscopePotassium channelsBirthday candlesBiological surfacesCellsBiomembranesMolecular structureRecent dataLocalization of amiloride-sensitive sodium channels in A6 cells by atomic force microscopy
Smith P, Bradford A, Schneider S, Benos D, Geibel J. Localization of amiloride-sensitive sodium channels in A6 cells by atomic force microscopy. American Journal Of Physiology 1997, 272: c1295-c1298. PMID: 9142855, DOI: 10.1152/ajpcell.1997.272.4.c1295.Peer-Reviewed Original ResearchConceptsAtomic force microscopyHigh-resolution imagingRenal epithelial cellsForce microscopyA6 cellsApical microvilliCell surface distributionBovine renal medullaEpithelial cellsA6 renal epithelial cellsAmiloride-sensitive sodium channelsEpithelial ion channelsColloidal gold particlesApical distributionIon channelsChannel complexGold particlesSodium channelsCellsMicrovilliNonimmune immunoglobulin GUnlabeled cellsMicroscopySurface distributionParticles
1995
Fluid absorption in isolated perfused colonic crypts.
Singh S, Binder H, Boron W, Geibel J. Fluid absorption in isolated perfused colonic crypts. Journal Of Clinical Investigation 1995, 96: 2373-2379. PMID: 7593625, PMCID: PMC185889, DOI: 10.1172/jci118294.Peer-Reviewed Original ResearchConceptsCrypt cellsNM vasoactive intestinal peptideFluid absorptionLarge intestinal functionColonic cryptsVasoactive intestinal peptideLamina propria cellsNet fluid secretionRat distal colonNet fluid absorptionDibutyryl cyclic AMPIntestinal peptideDistal colonIntestinal functionLamina propriaPathophysiological paradigmCrypt preparationMM acetylcholineBasal stateNeurohumoral agonistsFluid secretionCellular elementsEpithelial cellsIntestinal cryptsCrypts