2007
Characteristics of rat downregulated in adenoma (rDRA) expressed in HEK 293 cells
Barmeyer C, Ye JH, Sidani S, Geibel J, Binder HJ, Rajendran VM. Characteristics of rat downregulated in adenoma (rDRA) expressed in HEK 293 cells. Pflügers Archiv - European Journal Of Physiology 2007, 454: 441-450. PMID: 17492310, DOI: 10.1007/s00424-007-0213-7.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmino Acid SequenceAnimalsAntiportersBase SequenceBicarbonatesCell LineChloridesCloning, MolecularColonDNA PrimersFatty Acids, VolatileHumansHydrogen-Ion ConcentrationIon TransportMolecular Sequence DataRatsRecombinant ProteinsSequence Homology, Amino AcidSulfate TransportersTransfectionConceptsRat distal colonDistal colonHEK-293 cellsExtracellular Cl- concentrationCrypt cellsMRNA expressionColonRatsDigestive tractCharacteristics of ratsAdenomasHigh rateDIDSCl- influxSurface cellsIntracellular acidApical membrane vesiclesCellsDisulfonic acidApical membraneCl- uptakePrior observationsUptakeLungJejunum
2005
l-Type amino acids stimulate gastric acid secretion by activation of the calcium-sensing receptor in parietal cells
Busque SM, Kerstetter JE, Geibel JP, Insogna K. l-Type amino acids stimulate gastric acid secretion by activation of the calcium-sensing receptor in parietal cells. AJP Gastrointestinal And Liver Physiology 2005, 289: g664-g669. PMID: 15961860, DOI: 10.1152/ajpgi.00096.2005.Peer-Reviewed Original ResearchConceptsCalcium-sensing receptorAbsence of secretagoguesAcid secretionParietal cellsHistamine-2 receptorSystem L amino acid transportGastric acid secretionWhole stomach preparationDose-dependent increaseIndividual parietal cellsStomach preparationRat gastric glandsHEK-293 cellsCaSRL-amino acidsGastric glandsHormonal stimulationATPase activitySecretionPhysiological concentrationsReceptorsSecretagoguesAcid transportAmino acidsSecretory cells
2003
Intracellular pH Activates Membrane-Bound Na+/H+ Exchanger and Vacuolar H+-ATPase in Human Embryonic Kidney (HEK) Cells
Lang K, Wagner C, Haddad G, Burnekova O, Geibel J. Intracellular pH Activates Membrane-Bound Na+/H+ Exchanger and Vacuolar H+-ATPase in Human Embryonic Kidney (HEK) Cells. Cellular Physiology And Biochemistry 2003, 13: 257-262. PMID: 14586169, DOI: 10.1159/000074540.Peer-Reviewed Original ResearchConceptsMicroM EIPAIntracellular acidificationFree bath solutionProximal tubule cellsInfluence of intracellularWestern blot analysisTime-dependent mannerTubule cellsHuman embryonic kidney cellsHEK-293 cellsExtruding mechanismHuman embryonic kidney cell line HEK-293NHE3 proteinEmbryonic kidney cellsCell line HEK-293Cytosolic pHKidney cellsBlot analysisAbsence of bicarbonateHEK293 cellsBath solutionEIPAPresent studyActivationCells