2017
Stomach and Bone
Kitay AM, Geibel JP. Stomach and Bone. Advances In Experimental Medicine And Biology 2017, 1033: 97-131. PMID: 29101653, DOI: 10.1007/978-3-319-66653-2_6.Peer-Reviewed Original ResearchConceptsBone metabolismGastric acid secretionCalcium-sensing receptorVitamin DParathyroid glandsAcid secretionGastrointestinal organsTherapeutic implicationsEndocrine modulatorsCalcium homeostasisDiseaseStomachOrgansMetabolismImportant playersIntermediary organDiverse tissuesDysfunctionCaSRPTHCalcitoninKidneySecretionIntestineReceptors
2014
A Novel Device to Preserve Intestinal Tissue Ex-Vivo by Cold Peristaltic Perfusion
Narayan RR, Pancer NE, Loeb BW, Oki K, Crouch A, Backus S, Chauhan Y, Patrón-Lozano R, Rodriguez-Davalos MI, Geibel JP, Fan RE, Zinter JP. A Novel Device to Preserve Intestinal Tissue Ex-Vivo by Cold Peristaltic Perfusion. Annual International Conference Of The IEEE Engineering In Medicine And Biology Society (EMBC) 2014, 2014: 3118-3121. PMID: 25570651, DOI: 10.1109/embc.2014.6944283.Peer-Reviewed Original Research
2011
The Anion Exchanger Pendrin (SLC26A4) and Renal Acid-base Homeostasis
Wagner CA, Mohebbi N, Capasso G, Geibel JP. The Anion Exchanger Pendrin (SLC26A4) and Renal Acid-base Homeostasis. Cellular Physiology And Biochemistry 2011, 28: 497-504. PMID: 22116363, DOI: 10.1159/000335111.Peer-Reviewed Original ResearchConceptsAcid-base homeostasisAnion exchanger pendrinExchanger pendrinRenal acid-base homeostasisDietary electrolyte intakeSystemic acid-base statusRole of pendrinType BNormal acid-base homeostasisRegulation of pendrinAcid-base statusRenal expressionAngiotensin IIKidney's roleElectrolyte intakePendrin expressionPositive cellsExchanger activityGenetic ablationAdult kidneyPendrinExpressing cellsLuminal poleKidneyHomeostasis
2004
Renal Vacuolar H+-ATPase
Wagner CA, Finberg KE, Breton S, Marshansky V, Brown D, Geibel JP. Renal Vacuolar H+-ATPase. Physiological Reviews 2004, 84: 1263-1314. PMID: 15383652, DOI: 10.1152/physrev.00045.2003.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsProximal tubular bicarbonate reabsorptionDistal renal tubular acidosisTubular bicarbonate reabsorptionRenal tubular acidosisFinal urinary acidificationTubular acidosisElectrolyte statusBicarbonate reabsorptionMonogenic defectsProximal tubulesUrinary acidificationKidneyATP-dependent transportVariety of factorsHigher numberPatientsIntracellular organellesAcidosisRegulatory proteinsLocalization patterns
2003
Localization and Regulation of the ATP6V0A4 (a4) Vacuolar H+-ATPase Subunit Defective in an Inherited Form of Distal Renal Tubular Acidosis
Stehberger PA, Schulz N, Finberg KE, Karet FE, Giebisch G, Lifton RP, Geibel JP, Wagner CA. Localization and Regulation of the ATP6V0A4 (a4) Vacuolar H+-ATPase Subunit Defective in an Inherited Form of Distal Renal Tubular Acidosis. Journal Of The American Society Of Nephrology 2003, 14: 3027-3038. PMID: 14638902, DOI: 10.1097/01.asn.0000099375.74789.ab.Peer-Reviewed Original ResearchConceptsDistal renal tubular acidosisRenal tubular acidosisLoop of HenleProximal tubulesTubular acidosisMouse kidneyA4 expressionDistal tubulesDistal convoluted tubuleProtein expression levelsElectrolyte intakeA4 proteinConvoluted tubulesMouse nephron segmentsNephron segmentsApical stainingWestern blottingAcidosisProtein expressionKidneyATPase activityDistal portionHenleExpression levelsTubulesMolecular cloning and characterization of Atp6v1b1, the murine vacuolar H+-ATPase B1-subunit
Finberg KE, Wagner CA, Stehberger PA, Geibel JP, Lifton RP. Molecular cloning and characterization of Atp6v1b1, the murine vacuolar H+-ATPase B1-subunit. Gene 2003, 318: 25-34. PMID: 14585495, DOI: 10.1016/s0378-1119(03)00790-x.Peer-Reviewed Original ResearchMeSH Keywords5' Flanking RegionAmino Acid SequenceAnimalsAntibody SpecificityBase SequenceCloning, MolecularDNADNA, ComplementaryEpididymisGene Expression Regulation, EnzymologicHumansImmune SeraImmunohistochemistryIsoenzymesKidneyMaleMiceMice, Inbred StrainsMolecular Sequence DataPhylogenyProtein SubunitsRNA, MessengerSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidSequence Homology, Nucleic AcidVacuolar Proton-Translocating ATPasesConceptsDistal renal tubular acidosisRenal tubular acidosisMouse renal cortexProton-translocating ATPasesTubular acidosisRenal cortexSubset of tissuesAnimal modelsATP6V1B1Major organsGenomic organizationGenomic lociMouse kidneyProtein levelsMolecular cloningAcid proteinPlasma membraneMurine orthologIntracellular organellesB1 isoformKidneyNorthern blottingA rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse
Wagner CA, Lükewille U, Valles P, Breton S, Brown D, Giebisch GH, Geibel JP. A rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse. Pflügers Archiv - European Journal Of Physiology 2003, 446: 623-632. PMID: 12748863, DOI: 10.1007/s00424-003-1082-3.Peer-Reviewed Original ResearchConceptsProximal tubulesTubule fragmentsMouse kidneyKidney tubule fragmentsCollagenase digestion techniqueMouse proximal tubulesUnits/minTrypan blue exclusionKidney functionInner stripeOuter medullaIntercalated cellsIndependent intracellularBlue exclusionTrypan blueTubulesKidneyMicePH-sensitive dyeLight microscopyDuctAnimalsFurther characterizationATPase activityFunctional characteristics
2002
Regulation of the expression of the Cl-/anion exchanger pendrin in mouse kidney by acid-base status
Wagner CA, Finberg KE, Stehberger PA, Lifton RP, Giebisch GH, Aronson PS, Geibel JP. Regulation of the expression of the Cl-/anion exchanger pendrin in mouse kidney by acid-base status. Kidney International 2002, 62: 2109-2117. PMID: 12427135, DOI: 10.1046/j.1523-1755.2002.00671.x.Peer-Reviewed Original ResearchConceptsPendrin-positive cellsAcid-base statusPositive cellsBicarbonate secretionMouse kidneyAcid-base transportKnockout mouse modelProtein expression levelsMetabolic alkalosisDeficient dietExchanger pendrinPendrin expressionMouse modelSensorineural deafnessThyroid glandBicarbonate loadPendred syndromeWestern blottingApical membraneInner earPendrin proteinControl levelsKidneyPendrinProtein levels
2000
Cell pH and H+ Secretion by S3 Segment of Mammalian Kidney: Role of H+-ATPase and Cl−
Malnic G, Geibel J. Cell pH and H+ Secretion by S3 Segment of Mammalian Kidney: Role of H+-ATPase and Cl−. The Journal Of Membrane Biology 2000, 178: 115-125. PMID: 11083900, DOI: 10.1007/s002320010019.Peer-Reviewed Original ResearchConceptsProximal tubule cellsChannel blocker NPPBS3 segmentTubule cellsProximal S3 segmentsFree mediumCell pHConfocal microscopyNIH Image programCell pH regulationMicroperfusion techniqueSuperfusionMouse kidneyRabbit kidneyKidneySubcellular vesiclesApical areaCell acidificationMammalian kidneyFluorescence densityNPPBRingerCellsImage programNaCl Ringer
1990
Angiotensin II stimulates both Na(+)-H+ exchange and Na+/HCO3- cotransport in the rabbit proximal tubule.
Geibel J, Giebisch G, Boron W. Angiotensin II stimulates both Na(+)-H+ exchange and Na+/HCO3- cotransport in the rabbit proximal tubule. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 7917-7920. PMID: 2172967, PMCID: PMC54862, DOI: 10.1073/pnas.87.20.7917.Peer-Reviewed Original ResearchConceptsAngiotensin IIHCO3- reabsorptionProximal tubulesEffect of AIIS1 proximal tubulesNM angiotensin IIPH-sensitive fluorescent dye 2Rat proximal tubuleRabbit proximal tubuleSuperficial nephronsPotent stimulusBasolateral administrationRabbit kidneyFluorescent dye 2Basolateral solutionBasolateral stepTransporter activityTubulesIonic mechanismsCotransportKidneyAdministrationNephronA potassium channel in the apical membrane of rabbit thick ascending limb of Henle's loop
Wang W, White S, Geibel J, Giebisch G. A potassium channel in the apical membrane of rabbit thick ascending limb of Henle's loop. American Journal Of Physiology 1990, 258: f244-f253. PMID: 2309888, DOI: 10.1152/ajprenal.1990.258.2.f244.Peer-Reviewed Original ResearchConceptsCell-attached patchesHenle's loopPipette solutionPotassium channelsOutward slope conductanceRabbit kidneyBath solutionSlope conductanceThick ascending limbChannel activityPatch-clamp techniqueInward slope conductanceApical membraneVoltage-dependent mannerAscending limbSingle potassium channelsKidneyPossible intracellular regulatorsIntracellular regulatorsOpen probabilityLimb
1989
Mechanism of aldosterone-induced increase of K+ conductance in early distal renal tubule cells of the frog
Wang W, Henderson R, Geibel J, White S, Giebisch G. Mechanism of aldosterone-induced increase of K+ conductance in early distal renal tubule cells of the frog. The Journal Of Membrane Biology 1989, 111: 277-289. PMID: 2557452, DOI: 10.1007/bf01871012.Peer-Reviewed Original ResearchConceptsEarly distal tubule cellsTubule cellsDistal renal tubule cellsFrog kidneyWhole-cell voltage-clamp techniqueRemoval of aldosteronePresence of aldosteroneAldosterone-induced increaseType II channelsDistal tubule cellsOuabain-treated cellsPatch-clamp techniqueRenal tubule cellsVoltage-clamp techniquePresence of furosemideAldosterone treatmentAldosteroneIncubation of cellsKidneyOpen probabilityDistal cellsHrCellsIntracellularComplete absence