2013
CFTR and V‐ATPase trafficking and function in the intestinal brush border membrane
Collaco A, Geibel P, Kravtsov D, Ghantaphang S, Lee B, Geibel J, Ameen N. CFTR and V‐ATPase trafficking and function in the intestinal brush border membrane. The FASEB Journal 2013, 27: 913.10-913.10. DOI: 10.1096/fasebj.27.1_supplement.913.10.Peer-Reviewed Original ResearchES cellsV-ATPaseMulti-subunit proton pumpCystic fibrosis transmembrane conductance regulator (CFTR) chloride channelV-ATPase traffickingIntestinal brush border membraneBrush border membraneBasolateral domainPlasma membraneVacuolar ATPaseE subunitBorder membraneCAMP stimulationProton pumpCAMP activationDistinct domainsFunctional synergyApical membraneCFTRChloride channelsProton effluxSubunitsMouse intestineTransportersE interactionActivation of the Ca2+-sensing receptor induces deposition of tight junction components to the epithelial cell plasma membrane
Jouret F, Wu J, Hull M, Rajendran V, Mayr B, Schöfl C, Geibel J, Caplan MJ. Activation of the Ca2+-sensing receptor induces deposition of tight junction components to the epithelial cell plasma membrane. Journal Of Cell Science 2013, 126: 5132-5142. PMID: 24013548, PMCID: PMC3828589, DOI: 10.1242/jcs.127555.Peer-Reviewed Original ResearchConceptsTJ assemblyMDCK cellsStable transfectionFunction mutant formZO-1Tight junction components ZO-1G protein-coupled receptorsHuman CaSRCell-cell contactEpithelial cell plasma membranesMadin-Darby canine kidney cellsCell plasma membraneStimulation of CaSRDivalent ion homeostasisCanine kidney cellsTight junctionsJunction-associated proteinsTight junction componentsEndogenous CaSRProtein kinasePlasma membraneIon homeostasisMutant formsChelator BAPTA-AMCell differentiation
2009
The Phytostilbene Resveratrol Induces Apoptosis in INS-1E Rat Insulinoma Cells
Bortolotti C, Kunit T, Moder A, Hufnagl C, Schmidt S, Hartl A, Langelueddecke C, Fürst J, Geibel J, Ritter M, Jakab M. The Phytostilbene Resveratrol Induces Apoptosis in INS-1E Rat Insulinoma Cells. Cellular Physiology And Biochemistry 2009, 23: 245-254. PMID: 19471092, DOI: 10.1159/000218171.Peer-Reviewed Original ResearchConceptsINS-1E rat insulinoma cellsINS-1E cellsApoptotic volume decreaseRat insulinoma cellsAkt phosphorylationInsulinoma cellsCell proliferationProapoptotic effectsInhibitor compound CBasal Akt phosphorylationExposure of phosphatidylserineInduction of apoptosisG0/G1 phaseCaspase activationPlasma membraneOuter leafletCompound CMuM resveratrolG1 phaseCrystal violet stainingApoptotic cellsCell granularityAddition of insulinPhosphorylationMajor target
2005
The B1-subunit of the H+ ATPase is required for maximal urinary acidification
Finberg KE, Wagner CA, Bailey MA, Păunescu T, Breton S, Brown D, Giebisch G, Geibel JP, Lifton RP. The B1-subunit of the H+ ATPase is required for maximal urinary acidification. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 13616-13621. PMID: 16174750, PMCID: PMC1224669, DOI: 10.1073/pnas.0506769102.Peer-Reviewed Original ResearchConceptsMaximal urinary acidificationNormal urinary acidificationMetabolic acidosisUrinary acidificationDistal renal tubular acidosisAcid challengeRenal tubular acidosisAcute intracellular acidificationB1 subunitLumen-negative potentialFurosemide infusionTubular acidosisAlkaline urineDistal nephronGreater severityAcidosisMiceLoss of B1Intracellular acidificationApical expressionUrineDuctFurther decreaseIsoformsPlasma membraneInsulin Receptor (IR) and Glucose Transporter 2 (GLUT2) Proteins Form a Complex on the Rat Hepatocyte Membrane
Eisenberg M, Maker A, Slezak L, Nathan J, Sritharan K, Jena B, Geibel J, Andersen D. Insulin Receptor (IR) and Glucose Transporter 2 (GLUT2) Proteins Form a Complex on the Rat Hepatocyte Membrane. Cellular Physiology And Biochemistry 2005, 15: 051-058. PMID: 15665515, DOI: 10.1159/000083638.Peer-Reviewed Original ResearchConceptsGlucose transporter 2Insulin receptorPlasma membranePlasma membrane insulin receptorsFluorescence resonance energy transfer studiesMembrane insulin receptorsHepatic glucose transportersGlucose transporterHepatocyte plasma membraneHepatic glucose regulationInsulin regulationGlucose transportBidirectional glucose transportRat hepatocyte membranesConfocal microscopyTransporter 2MembraneEnergy transfer studiesRegulationTexas RedComplexesEndosomesCytosolProteinTransporters
2003
Molecular cloning and characterization of Atp6v1b1, the murine vacuolar H+-ATPase B1-subunit
Finberg KE, Wagner CA, Stehberger PA, Geibel JP, Lifton RP. Molecular cloning and characterization of Atp6v1b1, the murine vacuolar H+-ATPase B1-subunit. Gene 2003, 318: 25-34. PMID: 14585495, DOI: 10.1016/s0378-1119(03)00790-x.Peer-Reviewed Original ResearchMeSH Keywords5' Flanking RegionAmino Acid SequenceAnimalsAntibody SpecificityBase SequenceCloning, MolecularDNADNA, ComplementaryEpididymisGene Expression Regulation, EnzymologicHumansImmune SeraImmunohistochemistryIsoenzymesKidneyMaleMiceMice, Inbred StrainsMolecular Sequence DataPhylogenyProtein SubunitsRNA, MessengerSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidSequence Homology, Nucleic AcidVacuolar Proton-Translocating ATPasesConceptsDistal renal tubular acidosisRenal tubular acidosisMouse renal cortexProton-translocating ATPasesTubular acidosisRenal cortexSubset of tissuesAnimal modelsATP6V1B1Major organsGenomic organizationGenomic lociMouse kidneyProtein levelsMolecular cloningAcid proteinPlasma membraneMurine orthologIntracellular organellesB1 isoformKidneyNorthern blotting
2002
Calcium-pump inhibitors induce functional surface expression of ΔF508-CFTR protein in cystic fibrosis epithelial cells
Egan ME, Glöckner-Pagel J, Ambrose C, Cahill PA, Pappoe L, Balamuth N, Cho E, Canny S, Wagner CA, Geibel J, Caplan MJ. Calcium-pump inhibitors induce functional surface expression of ΔF508-CFTR protein in cystic fibrosis epithelial cells. Nature Medicine 2002, 8: 485-492. PMID: 11984593, DOI: 10.1038/nm0502-485.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumCalcium pump inhibitorΔF508-CFTR proteinCystic fibrosis epithelial cellsCystic fibrosis transmembrane conductance regulator (CFTR) proteinCystic fibrosis cell lineFunctional surface expressionSurface expressionChaperone activityChaperone proteinsRegulator proteinPlasma membraneCystic fibrosis defectCell surfaceProteinCell linesPotential targetOptimal activityInhibitor thapsigarginEpithelial cellsExpressionCommon mutationsInhibitorsMouse modelReticulum
1998
Sorting of P-type ATPases in polarized epithelial cells.
Dunbar LA, Courtois-Coutry N, Roush DL, Muth TR, Gottardi CJ, Rajendran V, Geibel J, Kashgarian M, Caplan MJ. Sorting of P-type ATPases in polarized epithelial cells. Acta Physiologica Scandinavica. Supplementum 1998, 643: 289-95. PMID: 9789572.Peer-Reviewed Original ResearchConceptsApical localizationK-ATPaseMost epithelial cell typesTyrosine-based signalsP-type familyP-type ATPasesEpithelial cellsCritical tyrosine residuesApical plasma membraneFourth transmembrane domainBeta-subunit sequencesApical cell surfaceEpithelial cell typesSorting signalsTransmembrane domainCytoplasmic tailSequence domainsPlasma membraneHomologous membersTyrosine residuesParietal cellsStorage compartmentCell typesCell surfaceBasolateral surface
1997
Gi regulation of secretory vesicle swelling examined by atomic force microscopy
Jena B, Schneider S, Geibel J, Webster P, Oberleithner H, Sritharan K. Gi regulation of secretory vesicle swelling examined by atomic force microscopy. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 13317-13322. PMID: 9371843, PMCID: PMC24306, DOI: 10.1073/pnas.94.24.13317.Peer-Reviewed Original ResearchConceptsSecretory vesicle membraneVesicle swellingSecretory vesiclesPlasma membraneVesicle fusionVesicle membraneSecretory vesicle swellingSecretory vesicle fusionConfocal microscopyZymogen granulesCell plasma membraneVesicle volumeHeterotrimeric guanineHeterotrimeric GTPZymogen granule sizeGTPase activityAlpha subunitMolecular mechanismsRegulatory rolePresence of GTPExocytotic processMastoparan analogsProteinGi proteinsMas7RAPID ALDOSTERONE‐INDUCED CELL VOLUME INCREASE OF ENDOTHELIAL CELLS MEASURED BY THE ATOMIC FORCE MICROSCOPE
SCHNEIDER S, YANO Y, SUMPIO B, JENA B, GEIBEL J, GEKLE M, OBERLEITHNER H. RAPID ALDOSTERONE‐INDUCED CELL VOLUME INCREASE OF ENDOTHELIAL CELLS MEASURED BY THE ATOMIC FORCE MICROSCOPE. Cell Biology International 1997, 21: 759-768. PMID: 9768474, DOI: 10.1006/cbir.1997.0220.Peer-Reviewed Original ResearchConceptsEndothelial cellsAddition of aldosteroneArterial blood pressureNon-genomic effectsCultured endothelial cellsBlood pressureMineralocorticoid hormonesHormone exposureAldosteroneCell volumeHuman leukocytesIntracellular electrolytesTransient swellingTriphosphate productionIntracellular pHLeukocytesVolume increaseBovine aortaContinuous presenceCellsPlasma membraneSurface dynamics in living acinar cells imaged by atomic force microscopy: Identification of plasma membrane structures involved in exocytosis
Schneider S, Sritharan K, Geibel J, Oberleithner H, Jena B. Surface dynamics in living acinar cells imaged by atomic force microscopy: Identification of plasma membrane structures involved in exocytosis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 316-321. PMID: 8990206, PMCID: PMC19328, DOI: 10.1073/pnas.94.1.316.Peer-Reviewed Original ResearchConceptsAtomic force microscopePlasma membrane structureForce microscopeLive acinar cellsSecretory vesicle dockingPlasma membrane dynamicsAtomic force microscopyAcinar cellsMembrane structureVesicle dockingCompensatory endocytosisExocytic processForce microscopyFreeze-fracture electron microscopyMembrane dynamicsPlasma membraneFusion poreUltrahigh resolutionIsolated pancreatic acinar cellsPancreatic acinar cellsSurface dynamicsBasolateral regionStimulation of secretionLimited resolutionElectron microscopy
1993
Imaging the lamellipodium of migrating epithelial cells in vivo by atomic force microscopy
Oberleithner H, Giebisch G, Geibel J. Imaging the lamellipodium of migrating epithelial cells in vivo by atomic force microscopy. Pflügers Archiv - European Journal Of Physiology 1993, 425: 506-510. PMID: 8134267, DOI: 10.1007/bf00374878.Peer-Reviewed Original ResearchConceptsPlasma membraneCell locomotionNew plasma membraneCell surfacePlasma membrane potentialCanine kidney cell linePlasma membrane integrityMadin-Darby canine kidney (MDCK) cell lineDynamic physiological processesKidney cell lineAlkaline stressInvagination processCytoskeletal elementsMDCK-F cellsPhysiological processesMembrane integrityMembrane turnoverCell linesKidney cellsMembrane potentialEpithelial cellsTurnover processCellsMembranePotential role