2003
Intracellular pH Activates Membrane-Bound Na+/H+ Exchanger and Vacuolar H+-ATPase in Human Embryonic Kidney (HEK) Cells
Lang K, Wagner C, Haddad G, Burnekova O, Geibel J. Intracellular pH Activates Membrane-Bound Na+/H+ Exchanger and Vacuolar H+-ATPase in Human Embryonic Kidney (HEK) Cells. Cellular Physiology And Biochemistry 2003, 13: 257-262. PMID: 14586169, DOI: 10.1159/000074540.Peer-Reviewed Original ResearchConceptsMicroM EIPAIntracellular acidificationFree bath solutionProximal tubule cellsInfluence of intracellularWestern blot analysisTime-dependent mannerTubule cellsHuman embryonic kidney cellsHEK-293 cellsExtruding mechanismHuman embryonic kidney cell line HEK-293NHE3 proteinEmbryonic kidney cellsCell line HEK-293Cytosolic pHKidney cellsBlot analysisAbsence of bicarbonateHEK293 cellsBath solutionEIPAPresent studyActivationCells
2000
Cell pH and H+ Secretion by S3 Segment of Mammalian Kidney: Role of H+-ATPase and Cl−
Malnic G, Geibel J. Cell pH and H+ Secretion by S3 Segment of Mammalian Kidney: Role of H+-ATPase and Cl−. The Journal Of Membrane Biology 2000, 178: 115-125. PMID: 11083900, DOI: 10.1007/s002320010019.Peer-Reviewed Original ResearchConceptsProximal tubule cellsChannel blocker NPPBS3 segmentTubule cellsProximal S3 segmentsFree mediumCell pHConfocal microscopyNIH Image programCell pH regulationMicroperfusion techniqueSuperfusionMouse kidneyRabbit kidneyKidneySubcellular vesiclesApical areaCell acidificationMammalian kidneyFluorescence densityNPPBRingerCellsImage programNaCl Ringer