2011
Functional role of NHE4 as a pH regulator in rat and human colonic crypts
Arena EA, Longo WE, Roberts KE, Geibel P, Nateqi J, Brandstetter M, Geibel JP. Functional role of NHE4 as a pH regulator in rat and human colonic crypts. American Journal Of Physiology - Cell Physiology 2011, 302: c412-c418. PMID: 22049213, DOI: 10.1152/ajpcell.00163.2011.Peer-Reviewed Original ResearchConceptsHuman colonic cryptsNHE4 activityColonic cryptsElective colon resectionEffects of aldosteroneSprague-Dawley ratsColon resectionRat modelGastrointestinal tractAldosteroneColonic samplesΜM forskolinFluid homeostasisRatsHuman colonHuman specimensCAMP levelsIntracellular pathwaysCryptsNHE4ColonSignificant changesLittle descriptionFunctional roleRecent studiesThe flavonone naringenin inhibits chloride secretion in isolated colonic epithelia
Collins D, Kopic S, Geibel JP, Hogan AM, Medani M, Baird AW, Winter DC. The flavonone naringenin inhibits chloride secretion in isolated colonic epithelia. European Journal Of Pharmacology 2011, 668: 271-277. PMID: 21762688, DOI: 10.1016/j.ejphar.2011.06.052.Peer-Reviewed Original ResearchConceptsHuman colonic mucosaChloride secretionAntisecretory actionColonic mucosaCo-transporter inhibitorsEffect of naringeninIntracellular cAMP generationColonic cell lineAntisecretory effectColonic functionColorectal cancerProtective effectUssing chambersColonic epitheliumSecretory functionCAMP generationInhibitory actionBasolateral NKCC1Human colonRatsSecretionIntracellular acidificationConflicting resultsCell linesMucosa
2007
Characteristics of rat downregulated in adenoma (rDRA) expressed in HEK 293 cells
Barmeyer C, Ye JH, Sidani S, Geibel J, Binder HJ, Rajendran VM. Characteristics of rat downregulated in adenoma (rDRA) expressed in HEK 293 cells. Pflügers Archiv - European Journal Of Physiology 2007, 454: 441-450. PMID: 17492310, DOI: 10.1007/s00424-007-0213-7.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmino Acid SequenceAnimalsAntiportersBase SequenceBicarbonatesCell LineChloridesCloning, MolecularColonDNA PrimersFatty Acids, VolatileHumansHydrogen-Ion ConcentrationIon TransportMolecular Sequence DataRatsRecombinant ProteinsSequence Homology, Amino AcidSulfate TransportersTransfectionConceptsRat distal colonDistal colonHEK-293 cellsExtracellular Cl- concentrationCrypt cellsMRNA expressionColonRatsDigestive tractCharacteristics of ratsAdenomasHigh rateDIDSCl- influxSurface cellsIntracellular acidApical membrane vesiclesCellsDisulfonic acidApical membraneCl- uptakePrior observationsUptakeLungJejunum