2003
A rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse
Wagner CA, Lükewille U, Valles P, Breton S, Brown D, Giebisch GH, Geibel JP. A rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse. Pflügers Archiv - European Journal Of Physiology 2003, 446: 623-632. PMID: 12748863, DOI: 10.1007/s00424-003-1082-3.Peer-Reviewed Original ResearchConceptsProximal tubulesTubule fragmentsMouse kidneyKidney tubule fragmentsCollagenase digestion techniqueMouse proximal tubulesUnits/minTrypan blue exclusionKidney functionInner stripeOuter medullaIntercalated cellsIndependent intracellularBlue exclusionTrypan blueTubulesKidneyMicePH-sensitive dyeLight microscopyDuctAnimalsFurther characterizationATPase activityFunctional characteristics
1998
Angiotensin II stimulates vesicular H+-ATPase in rat proximal tubular cells
Wagner C, Giebisch G, Lang F, Geibel J. Angiotensin II stimulates vesicular H+-ATPase in rat proximal tubular cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 9665-9668. PMID: 9689138, PMCID: PMC21396, DOI: 10.1073/pnas.95.16.9665.Peer-Reviewed Original ResearchConceptsAngiotensin IIProximal tubulesIon secretionEffect of AIIRat proximal tubular cellsAngiotensin II stimulatesRemoval of ClPH-sensitive fluorescent dye 2Proximal tubular cellsNa/H exchangerBafilomycin A1Proximal tubule fragmentsTubular cellsBicarbonate reabsorptionHCO3- reabsorptionTubule fragmentsH exchangerATPase activityFluorescent dye 2Proton pump inhibitor bafilomycin A1Intracellular pHTubulesSecretionPresent studyCell pH