2015
Nanoparticles that deliver triplex-forming peptide nucleic acid molecules correct F508del CFTR in airway epithelium
McNeer NA, Anandalingam K, Fields RJ, Caputo C, Kopic S, Gupta A, Quijano E, Polikoff L, Kong Y, Bahal R, Geibel JP, Glazer PM, Saltzman WM, Egan ME. Nanoparticles that deliver triplex-forming peptide nucleic acid molecules correct F508del CFTR in airway epithelium. Nature Communications 2015, 6: 6952. PMID: 25914116, PMCID: PMC4480796, DOI: 10.1038/ncomms7952.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineChloridesCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorDNA-Binding ProteinsGenetic TherapyHigh-Throughput Nucleotide SequencingHumansLactic AcidMice, Inbred C57BLNanoparticlesPeptide Nucleic AcidsPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerPolymersRespiratory MucosaConceptsFacile genome engineeringVivo gene deliveryBiodegradable polymer nanoparticlesTransient gene expressionNanoparticle systemsGene deliveryPolymer nanoparticlesGene correctionGenome engineeringNanoparticlesOff-target effectsPeptide nucleic acidLethal genetic disorderNucleic acidsDonor DNATarget effectsIntranasal deliveryDeliveryCystic fibrosisEngineeringOligonucleotideChloride effluxHuman cellsAirway epitheliumLung tissue
2000
Dendrimer-assisted patch-clamp sizing of nuclear pores
Bustamante J, Michelette E, Geibel J, Hanover J, McDonnell T, Dean D. Dendrimer-assisted patch-clamp sizing of nuclear pores. Pflügers Archiv - European Journal Of Physiology 2000, 439: 829-837. PMID: 10784359, PMCID: PMC4397974, DOI: 10.1007/s004249900233.Peer-Reviewed Original ResearchConceptsNuclear pore complexNuclear localization signalMacromolecular translocationFluorescence microscopy dataNuclear envelopeGreen fluorescence proteinLocalization signalPore complexNuclear poresMicroscopy dataColloidal gold particlesFluorescence proteinElectron microscopyCell lysatesFluorescence microscopyPhysiological conditionsDistinct classesCellular structureGold particlesProteinTranslocationUseful probeCardiomyocyte nucleiB-phycoerythrinParticles