2001
Effects of the Serine/Threonine Kinase SGK1 on the Epithelial Na+ Channel (ENaC) and CFTR: Implications for Cystic Fibrosis
Wagner C, Ott M, Klingel K, Beck S, Melzig J, Friedrich B, Wild K, Bröer S, Moschen I, Albers A, Waldegger S, Tümmler B, Egan M, Geibel J, Kandolf R, Lang F. Effects of the Serine/Threonine Kinase SGK1 on the Epithelial Na+ Channel (ENaC) and CFTR: Implications for Cystic Fibrosis. Cellular Physiology And Biochemistry 2001, 11: 209-218. PMID: 11509829, DOI: 10.1159/000051935.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAmino Acid SubstitutionAnimalsBronchiCell LineCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorEpithelial CellsEpithelial Sodium ChannelsHumansIn Situ HybridizationLungMacrophages, AlveolarMutationOocytesPatch-Clamp TechniquesProtein Serine-Threonine KinasesPulmonary AlveoliRNA, ComplementaryRNA, MessengerSodiumSodium ChannelsXenopus laevisConceptsSerine/threonine kinase SGK1Lung tissueCystic fibrosisCF patientsKinase SGK1CF lung tissueXenopus oocytesLoss of CFTRLung epithelial cell lineCoexpression of CFTREffect of SGK1Pathophysiological factorsEpithelial cell lineRespiratory epitheliumLung phenotypeVariety of stimuliCl(-) secretionSGK1 expressionInhibitor amilorideInhibitory effectEpithelial cellsEnhanced expressionChannel ENaC.CFTR mutationsChannel activity
1997
Localization of amiloride-sensitive sodium channels in A6 cells by atomic force microscopy
Smith P, Bradford A, Schneider S, Benos D, Geibel J. Localization of amiloride-sensitive sodium channels in A6 cells by atomic force microscopy. American Journal Of Physiology 1997, 272: c1295-c1298. PMID: 9142855, DOI: 10.1152/ajpcell.1997.272.4.c1295.Peer-Reviewed Original ResearchConceptsAtomic force microscopyHigh-resolution imagingRenal epithelial cellsForce microscopyA6 cellsApical microvilliCell surface distributionBovine renal medullaEpithelial cellsA6 renal epithelial cellsAmiloride-sensitive sodium channelsEpithelial ion channelsColloidal gold particlesApical distributionIon channelsChannel complexGold particlesSodium channelsCellsMicrovilliNonimmune immunoglobulin GUnlabeled cellsMicroscopySurface distributionParticles