2020
Penicillin G Induces H+, K+-ATPase via a Nitric Oxide-Dependent Mechanism in the Rat Colonic Crypt
Baratta VM, Norz V, Barahona MJ, Gisinger TM, Mulligan D, Geibel JP. Penicillin G Induces H+, K+-ATPase via a Nitric Oxide-Dependent Mechanism in the Rat Colonic Crypt. Cellular Physiology And Biochemistry 2020, 54: 1132-1142. PMID: 33175479, PMCID: PMC8095381, DOI: 10.33594/000000305.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArginineColonH(+)-K(+)-Exchanging ATPaseMaleNG-Nitroarginine Methyl EsterNitric OxidePenicillin GRatsRats, Sprague-DawleyConceptsRat colonic cryptsColonic cryptsNitric oxideNitric oxide-dependent mechanismPotassium homeostasisArginine methyl esterAmmonium prepulse techniqueIntracellular nitric oxideSodium-free conditionsL-NAMEDiarrheal statesATPase activityBACKGROUND/Presence of NOPenicillin G sodium saltAcute exposureL-arginineSmall intestineIsolated administrationN-nitroHomeostatic conditionsCryptsPrepulse techniqueSignificant reductionNovel mechanism
2018
Modulations in extracellular calcium lead to H+-ATPase-dependent acid secretion: a clarification of PPI failure
Kitay AM, Schneebacher MT, Schmitt A, Heschl K, Kopic S, Alfadda T, Alsaihati A, Link A, Geibel J. Modulations in extracellular calcium lead to H+-ATPase-dependent acid secretion: a clarification of PPI failure. AJP Gastrointestinal And Liver Physiology 2018, 315: g36-g42. PMID: 29517927, DOI: 10.1152/ajpgi.00132.2017.Peer-Reviewed Original ResearchConceptsAcid secretionParietal cellsExtracellular calciumGastric parietal cellsProton pump inhibitor exposureExtracellular CaPotassium-free conditionsRecurrent reflux symptomsCalcium sensing receptorGastric acid secretionPPI failurePPI therapyReflux symptomsIsolated gastric glandsInhibitor exposureCalcimimetic R568Pharmacological targetsSensing receptorAbsence of sodiumCalcium concentrationCaSRGastric glandsSecretionATPase activityExcretion pathway
2017
Activation of Secretagogue Independent Gastric Acid Secretion via Endothelial Nitric Oxide Synthase Stimulation in Rats
Kitay AM, Link A, Geibel JP. Activation of Secretagogue Independent Gastric Acid Secretion via Endothelial Nitric Oxide Synthase Stimulation in Rats. Cellular Physiology And Biochemistry 2017, 44: 1606-1615. PMID: 29212068, DOI: 10.1159/000485755.Peer-Reviewed Original ResearchConceptsNitric oxide synthaseGastric acid secretionAcid secretionL-arginineL-NAMENO/sGC/cGMP pathwayNitric oxideSGC/cGMP pathwayParietal cellsNitric oxide synthase stimulationSGC inhibitor ODQL-arginine exposureAcid-related diseasesPossible intracellular mechanismsNovel secretagogueRat parietal cellsIsolated gastric glandsUlcer diseaseOxide synthaseCGMP pathwayImmune functionImportant mediatorIntracellular mechanismsSecretionGastric glandsAcute Effects of Vitamin C Exposure On Colonic Crypts: Direct Modulation of pH Regulation
Aldajani MM, Vanicek CN, Alhazzaa N, Lysyy T, Agarwal R, Geibel JP. Acute Effects of Vitamin C Exposure On Colonic Crypts: Direct Modulation of pH Regulation. Cellular Physiology And Biochemistry 2017, 44: 377-387. PMID: 29132138, DOI: 10.1159/000484914.Peer-Reviewed Original ResearchConceptsVitamin CColonic cryptsAcute effectsDependent intracellular pH recoveryTumor formationIntracellular pH recoveryNew therapeutic agentsMM vitamin CColorectal cancerDistal colonLeading causeTranscellular passive diffusionBasolateral membraneAcute additionNormal colonic cryptsC exposureNutraceutical agentTherapeutic agentsPH recoveryIntracellular pH changesCancer cellsVitamin C.CryptsLow intracellularTight junctions
2016
Use of Fluorescein Isothiocyanate-Inulin as a Marker for Intestinal Ischemic Injury
AlKukhun A, Caturegli G, Munoz-Abraham AS, Judeeba S, Patron-Lozano R, Morotti R, Rodriguez-Davalos MI, Geibel JP. Use of Fluorescein Isothiocyanate-Inulin as a Marker for Intestinal Ischemic Injury. Journal Of The American College Of Surgeons 2016, 224: 1066-1073. PMID: 28017811, DOI: 10.1016/j.jamcollsurg.2016.12.016.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsFluorescein-5-isothiocyanateIntestinesInulinIschemiaMaleRatsRats, Sprague-DawleyConceptsFluorescein isothiocyanate-inulinIschemic injuryIntestinal ischemiaIntestinal transplantationFluid secretionIntestinal ischemic injuryIntestinal tissue samplesSmall intestine segmentsHEPES-Ringer solutionIntestinal graftMesenteric ischemiaIschemic intestineHistopathologic samplesExperimental armControl armControl intestineIschemic stateBlinded pathologistIschemiaIntestinal lumenIntestinal tissueIntestine segmentsScoring systemFluid shiftsInjurySecretagogue-dependent and -independent transport of zinc hydration forms in rat parietal cells
Ferstl FS, Kitay AM, Trattnig RM, Alsaihati A, Geibel JP. Secretagogue-dependent and -independent transport of zinc hydration forms in rat parietal cells. Pflügers Archiv - European Journal Of Physiology 2016, 468: 1877-1883. PMID: 27757581, DOI: 10.1007/s00424-016-1889-3.Peer-Reviewed Original ResearchConceptsGastroesophageal reflux diseaseAcid secretionParietal cellsSecretagogue-stimulated acid secretionRecurrence of symptomsAlternative treatment optionNumber of patientsNon-fasted conditionsApplication of bumetanideEffects of secretagoguesRat parietal cellsReflux diseaseInhibitor therapyTreatment optionsNon-fasted animalsLeading causeNeuronal activationNKCC1 transporterNeuronal pathwaysGastric acidInhibitor bumetanideSecretagogue carbacholFasted animalsMechanism of entryHormonal activation
2013
Functional vacuolar ATPase (V-ATPase) proton pumps traffic to the enterocyte brush border membrane and require CFTR
Collaco AM, Geibel P, Lee BS, Geibel JP, Ameen NA. Functional vacuolar ATPase (V-ATPase) proton pumps traffic to the enterocyte brush border membrane and require CFTR. American Journal Of Physiology - Cell Physiology 2013, 305: c981-c996. PMID: 23986201, PMCID: PMC4109618, DOI: 10.1152/ajpcell.00067.2013.Peer-Reviewed Original ResearchConceptsV-ATPaseCystic fibrosis transmembrane conductance regulator (CFTR) channelV-ATPase complexV-ATPase functionBrush border membraneProton effluxRat Brunner's glandsIntestinal cellsCAMP/PKACaco-2BBe cellsBorder membraneApical domainCoimmunoprecipitation studiesCFTR distributionVacuolar ATPasesSubapical cytoplasmSpecific subunitsCAMP stimulationProton pumpCAMP treatmentEnterocyte brush border membraneSodium-hydrogen exchangerHydrogen exchangerApical membraneCFTREffect of NSAIDs on Na+/H+ exchanger activity in rat colonic crypts
Roginiel AC, Kohut DL, Kaur S, Saleh AM, Weber T, Geibel P, Singh H, Geibel JP. Effect of NSAIDs on Na+/H+ exchanger activity in rat colonic crypts. American Journal Of Physiology - Cell Physiology 2013, 305: c512-c518. PMID: 23739181, DOI: 10.1152/ajpcell.00303.2012.Peer-Reviewed Original ResearchConceptsRat colonic cryptsExchanger activityNonsteroidal anti-inflammatory drugsUpper GI injuryColonic cryptsLower GI bleedingUse of NSAIDsChronic inflammatory stateLower GI tractUse of suppositoriesEffects of NSAIDsAnti-inflammatory drugsGI injuryNSAIDs aspirinGI bleedingPain reliefRheumatoid diseaseEpithelial erosionsInflammatory stateMyocardial infarctionChronic conditionsMucosal barrierUlcer formationColonic tissueGastrointestinal tractIdentification of Functionally Distinct Na-HCO3 Co-Transporters in Colon
Barmeyer C, Ye JH, Soroka C, Geibel P, Hingsammer LM, Weitgasser L, Atway D, Geibel JP, Binder HJ, Rajendran VM. Identification of Functionally Distinct Na-HCO3 Co-Transporters in Colon. PLOS ONE 2013, 8: e62864. PMID: 23690961, PMCID: PMC3653958, DOI: 10.1371/journal.pone.0062864.Peer-Reviewed Original ResearchConceptsNa-HCO3 cotransportDistal colonProximal colonNBC activityNBC isoformsRat colonColonic epithelial cellsCrypt glandsSensitive transporterHCO3- secretionColonHCO3-dependent mechanismEpithelial cellsSaturable processHOE694Isoform 1Basolateral membraneCotransportPCR analysisNorthern blotDIDSMolecular studiesMolecular identityApparent KiCritical roleThe calcium sensing receptor modulates fluid reabsorption and acid secretion in the proximal tubule
Capasso G, Geibel PJ, Damiano S, Jaeger P, Richards WG, Geibel JP. The calcium sensing receptor modulates fluid reabsorption and acid secretion in the proximal tubule. Kidney International 2013, 84: 277-284. PMID: 23615500, DOI: 10.1038/ki.2013.137.Peer-Reviewed Original ResearchMeSH KeywordsAcid-Base EquilibriumAnimalsCalcimimetic AgentsCalciumHydrogen-Ion ConcentrationIn Vitro TechniquesKidney Tubules, ProximalMaleMiceMice, KnockoutNuclear ProteinsPerfusionPuncturesRatsRats, Sprague-DawleyReceptors, Calcium-SensingReceptors, G-Protein-CoupledSodium-Hydrogen ExchangersTranscription FactorsConceptsProximal tubulesLuminal calcium concentrationFluid reabsorptionAcid secretionKnockout miceCalcium ion concentrationCalcium concentrationRole of CaSRActivation of CaSRCalcium sensing receptorCalcium-sensing receptorMouse proximal tubulesProton secretionSodium-hydrogen exchangerCalcimimetic agentBicarbonate absorptionSensing receptorCaSRFluid absorptionSecretionReabsorptionMiceCalcimimeticsTubulesLuminal membrane
2011
Hypoxia Inhibits Colonic Ion Transport via Activation of AMP Kinase
Collins D, Kopic S, Bachlechner J, Ritter M, Winter DC, Geibel JP. Hypoxia Inhibits Colonic Ion Transport via Activation of AMP Kinase. Annals Of Surgery 2011, 254: 957-963. PMID: 21562404, DOI: 10.1097/sla.0b013e31821d477f.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorChloride secretionMucosal hypoxiaAcute hypoxiaColonic electrolyte transportColonic mucosal functionIschemic intestinal injuryElectrogenic chloride secretionColonic ion transportPotential therapeutic targetHuman colonic mucosaIntracellular chloride concentrationRat colonic cryptsEpithelial ion transportFibrosis transmembrane conductance regulatorIschemic colitisIntestinal injuryColonic obstructionInhibition of CFTRMucosal functionAnastomotic failureAcute effectsColonic mucosaAdaptive cellular responseTransmembrane conductance regulatorVacuolar-type H+-ATPase-mediated proton transport in the rat parietal cell
Kopic S, Wagner ME, Griessenauer C, Socrates T, Ritter M, Geibel JP. Vacuolar-type H+-ATPase-mediated proton transport in the rat parietal cell. Pflügers Archiv - European Journal Of Physiology 2011, 463: 419-427. PMID: 22146938, DOI: 10.1007/s00424-011-1060-0.Peer-Reviewed Original ResearchConceptsV-ATPase activityV-ATPaseAcid secretionParietal cellsRat parietal cellsProton pump inhibitor therapyCertain specialized cellsK-ATPaseProton transportVacuolar-type H-ATPaseK-ATPase inhibitionSecretion pathwayTrafficking mechanismsSpecialized cellsMicrotubule networkActive acidificationInhibitor therapyIntracellular organellesK-ATPase activityRat gastric glandsConcanamycin APharmacological inhibitionAcid loadIntracellular alkalinizationAcid breakthroughFunctional role of NHE4 as a pH regulator in rat and human colonic crypts
Arena EA, Longo WE, Roberts KE, Geibel P, Nateqi J, Brandstetter M, Geibel JP. Functional role of NHE4 as a pH regulator in rat and human colonic crypts. American Journal Of Physiology - Cell Physiology 2011, 302: c412-c418. PMID: 22049213, DOI: 10.1152/ajpcell.00163.2011.Peer-Reviewed Original ResearchConceptsHuman colonic cryptsNHE4 activityColonic cryptsElective colon resectionEffects of aldosteroneSprague-Dawley ratsColon resectionRat modelGastrointestinal tractAldosteroneColonic samplesΜM forskolinFluid homeostasisRatsHuman colonHuman specimensCAMP levelsIntracellular pathwaysCryptsNHE4ColonSignificant changesLittle descriptionFunctional roleRecent studies
2010
Zinc Salts Provide a Novel, Prolonged and Rapid Inhibition of Gastric Acid Secretion
Kirchhoff P, Socrates T, Sidani S, Duffy A, Breidthardt T, Grob C, Viehl CT, Beglinger C, Oertli D, Geibel JP. Zinc Salts Provide a Novel, Prolonged and Rapid Inhibition of Gastric Acid Secretion. The American Journal Of Gastroenterology 2010, 106: 62. PMID: 20736941, DOI: 10.1038/ajg.2010.327.Peer-Reviewed Original ResearchConceptsProton pump inhibitorsGastric acid secretionAcid secretionRat gastric glandsRandomized cross-over studyIntact rat stomachAcid suppression therapyGastric glandsCross-over studyOnset of actionLong-term efficacyReturn of symptomsHealthy human volunteersSecretagogue-induced acid secretionStomach acid secretionProlonged therapySuppression therapyIntragastric pHPump inhibitorsSingle doseWhole stomachLifetime prevalenceRat modelRat stomachFaster onsetEthanol Inhibits Gastric Acid Secretion in Rats Through Increased AMP-Kinase Activity
Kopic S, Corradini S, Sidani S, Murek M, Vardanyan A, Föller M, Ritter M, Geibel JP. Ethanol Inhibits Gastric Acid Secretion in Rats Through Increased AMP-Kinase Activity. Cellular Physiology And Biochemistry 2010, 25: 195-202. PMID: 20110680, DOI: 10.1159/000276553.Peer-Reviewed Original ResearchConceptsGastric acid secretionAcid secretionSecretagogue-induced acid secretionInhibits gastric acid secretionShort-term ethanol exposurePH-sensitive dye BCECFLow-dose ethanolCompound CAMPK inhibitor compound CSpecific AMPK inhibitor compound CEffects of ethanolInhibitor compound CEthanol exposureRat gastric glandsActivation of AMPKInteraction of ethanolRat gastric parietal cellsFluorescence digital imagingGastric parietal cellsAMP kinase activityDye BCECFInhibitory effectSecretionParietal cellsAMPK pathway
2007
SLC26A7 Can function as a chloride-loading mechanism in parietal cells
Kosiek O, Busque SM, Föller M, Shcheynikov N, Kirchhoff P, Bleich M, Muallem S, Geibel JP. SLC26A7 Can function as a chloride-loading mechanism in parietal cells. Pflügers Archiv - European Journal Of Physiology 2007, 454: 989-998. PMID: 17404755, DOI: 10.1007/s00424-007-0254-y.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsAntiportersChloride-Bicarbonate AntiportersChloridesFluorescent Antibody TechniqueGastric AcidGastric MucosaImage Processing, Computer-AssistedIn Vitro TechniquesMaleOocytesParietal Cells, GastricPatch-Clamp TechniquesRatsRats, Sprague-DawleySodiumSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 2Sulfate TransportersXenopusConceptsParietal cellsXenopus laevis oocyte expression systemRat parietal cellsExpression of SLC26A7Rat gastric glandsAcid secretionOocyte expression systemNKCC inhibitorWestern blotHistamine stimulationPharmacological inhibitorsAcidic pHiGastric glandsDIDS concentrationExchanger AE2Subsequent readditionTransporter activityDisulfonic acidInhibitorsCellsSLC26A7InfluxImmunohistochemistrySecretagoguesUptake systemPI3 Kinase Dependent Stimulation of Gastric Acid Secretion by Dexamethasone
Lang P, Schniepp R, Kirchhoff P, Socrates T, Sidani S, Geibel J. PI3 Kinase Dependent Stimulation of Gastric Acid Secretion by Dexamethasone. Cellular Physiology And Biochemistry 2007, 20: 527-534. PMID: 17762179, DOI: 10.1159/000107536.Peer-Reviewed Original ResearchMeSH KeywordsAndrostadienesAnimalsCimetidineDexamethasoneGastric AcidGastric MucosaH(+)-K(+)-Exchanging ATPaseMaleNitrobenzoatesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPotassium Channel BlockersProtein Kinase InhibitorsProton Pump InhibitorsRatsRats, Sprague-DawleyStaurosporineWortmanninConceptsGastric acid secretionProtein kinase inhibitor staurosporinePI3-kinase inhibitor wortmanninAcid secretionKinase inhibitor staurosporineKinase inhibitor wortmanninPI3-kinase pathwayPeptic ulcerDexamethasone effectApical Cl- channelsKinase pathwayInhibitor wortmanninInhibitor staurosporineExcessive gastric acid secretionProton extrusionATPase inhibitor omeprazoleCl- channel blockers NPPBCl- channelsChannel blocker NPPBDependent stimulationDexamethasone injectionVivo pretreatmentInhibitor omeprazoleParietal cellsSecretion
2006
Characteristics of the K+-competitive H+,K+-ATPase inhibitor AZD0865 in isolated rat gastric glands
Kirchhoff P, Andersson K, Socrates T, Sidani S, Kosiek O, Geibel J. Characteristics of the K+-competitive H+,K+-ATPase inhibitor AZD0865 in isolated rat gastric glands. AJP Gastrointestinal And Liver Physiology 2006, 291: g838-g843. PMID: 16798725, DOI: 10.1152/ajpgi.00120.2006.Peer-Reviewed Original ResearchConceptsGastric acid secretionAcid secretionRat gastric glandsPotassium-competitive acid blockerGastric glandsPeptic ulcer diseaseProton pump inhibitor omeprazoleAcid-related diseasesCompetitive acid blockerPresence of omeprazoleLow-dose inhibitorsFree perfusateUlcer diseasePharmacological treatmentAZD0865High success rateAcid blockerLow doseRapid onsetSensitive dye BCECFInhibitor omeprazoleAcid loadOmeprazoleDye BCECFSecretion
2005
l-Type amino acids stimulate gastric acid secretion by activation of the calcium-sensing receptor in parietal cells
Busque SM, Kerstetter JE, Geibel JP, Insogna K. l-Type amino acids stimulate gastric acid secretion by activation of the calcium-sensing receptor in parietal cells. AJP Gastrointestinal And Liver Physiology 2005, 289: g664-g669. PMID: 15961860, DOI: 10.1152/ajpgi.00096.2005.Peer-Reviewed Original ResearchConceptsCalcium-sensing receptorAbsence of secretagoguesAcid secretionParietal cellsHistamine-2 receptorSystem L amino acid transportGastric acid secretionWhole stomach preparationDose-dependent increaseIndividual parietal cellsStomach preparationRat gastric glandsHEK-293 cellsCaSRL-amino acidsGastric glandsHormonal stimulationATPase activitySecretionPhysiological concentrationsReceptorsSecretagoguesAcid transportAmino acidsSecretory cellsRole of short-chain fatty acids in colonic HCO3 secretion
Vidyasagar S, Barmeyer C, Geibel J, Binder HJ, Rajendran VM. Role of short-chain fatty acids in colonic HCO3 secretion. AJP Gastrointestinal And Liver Physiology 2005, 288: g1217-g1226. PMID: 15677553, DOI: 10.1152/ajpgi.00415.2004.Peer-Reviewed Original ResearchConceptsShort-chain fatty acidsSCFA/SCFA transportApical membrane vesiclesMM butyrateSurface epithelial cellsFatty acidsSCFA metabolismColonic mucosaHCO3- secretionCrypt cellsCarrier-mediated processLucite chamberSecretionColonic cryptsEpithelial cellsComparable degreeButyrate stimulationChannel activityButyrate uptakeStat techniqueCryptsAnion channel activityDIDSButyrate