2020
Penicillin G Induces H+, K+-ATPase via a Nitric Oxide-Dependent Mechanism in the Rat Colonic Crypt
Baratta VM, Norz V, Barahona MJ, Gisinger TM, Mulligan D, Geibel JP. Penicillin G Induces H+, K+-ATPase via a Nitric Oxide-Dependent Mechanism in the Rat Colonic Crypt. Cellular Physiology And Biochemistry 2020, 54: 1132-1142. PMID: 33175479, PMCID: PMC8095381, DOI: 10.33594/000000305.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArginineColonH(+)-K(+)-Exchanging ATPaseMaleNG-Nitroarginine Methyl EsterNitric OxidePenicillin GRatsRats, Sprague-DawleyConceptsRat colonic cryptsColonic cryptsNitric oxideNitric oxide-dependent mechanismPotassium homeostasisArginine methyl esterAmmonium prepulse techniqueIntracellular nitric oxideSodium-free conditionsL-NAMEDiarrheal statesATPase activityBACKGROUND/Presence of NOPenicillin G sodium saltAcute exposureL-arginineSmall intestineIsolated administrationN-nitroHomeostatic conditionsCryptsPrepulse techniqueSignificant reductionNovel mechanism
2018
Modulations in extracellular calcium lead to H+-ATPase-dependent acid secretion: a clarification of PPI failure
Kitay AM, Schneebacher MT, Schmitt A, Heschl K, Kopic S, Alfadda T, Alsaihati A, Link A, Geibel J. Modulations in extracellular calcium lead to H+-ATPase-dependent acid secretion: a clarification of PPI failure. AJP Gastrointestinal And Liver Physiology 2018, 315: g36-g42. PMID: 29517927, DOI: 10.1152/ajpgi.00132.2017.Peer-Reviewed Original ResearchConceptsAcid secretionParietal cellsExtracellular calciumGastric parietal cellsProton pump inhibitor exposureExtracellular CaPotassium-free conditionsRecurrent reflux symptomsCalcium sensing receptorGastric acid secretionPPI failurePPI therapyReflux symptomsIsolated gastric glandsInhibitor exposureCalcimimetic R568Pharmacological targetsSensing receptorAbsence of sodiumCalcium concentrationCaSRGastric glandsSecretionATPase activityExcretion pathway
2017
Activation of Secretagogue Independent Gastric Acid Secretion via Endothelial Nitric Oxide Synthase Stimulation in Rats
Kitay AM, Link A, Geibel JP. Activation of Secretagogue Independent Gastric Acid Secretion via Endothelial Nitric Oxide Synthase Stimulation in Rats. Cellular Physiology And Biochemistry 2017, 44: 1606-1615. PMID: 29212068, DOI: 10.1159/000485755.Peer-Reviewed Original ResearchConceptsNitric oxide synthaseGastric acid secretionAcid secretionL-arginineL-NAMENO/sGC/cGMP pathwayNitric oxideSGC/cGMP pathwayParietal cellsNitric oxide synthase stimulationSGC inhibitor ODQL-arginine exposureAcid-related diseasesPossible intracellular mechanismsNovel secretagogueRat parietal cellsIsolated gastric glandsUlcer diseaseOxide synthaseCGMP pathwayImmune functionImportant mediatorIntracellular mechanismsSecretionGastric glands
2014
The Putative Role of the Non-Gastric H+/K+-ATPase ATP12A (ATP1AL1) as Anti-Apoptotic Ion Transporter: Effect of the H+/K+ ATPase Inhibitor SCH28080 on Butyrate-Stimulated Myelomonocytic HL-60 Cells
Jakab M, Hofer S, Ravasio A, Huber F, Schmidt S, Hitzl W, Geibel JP, Fürst J, Ritter M. The Putative Role of the Non-Gastric H+/K+-ATPase ATP12A (ATP1AL1) as Anti-Apoptotic Ion Transporter: Effect of the H+/K+ ATPase Inhibitor SCH28080 on Butyrate-Stimulated Myelomonocytic HL-60 Cells. Cellular Physiology And Biochemistry 2014, 34: 1507-1526. PMID: 25322912, DOI: 10.1159/000366355.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisButyratesCell Line, TumorCell MembraneH(+)-K(+)-Exchanging ATPaseHL-60 CellsHumansImidazolesIon TransportConceptsIntracellular acid loadHL-60 cellsAcute intracellular acid loadAcid loadMCV measurementsOsmotic cell shrinkageReal-time reverse transcription PCREarly apoptosisChanges of intracellularCD86 expressionFlow cytometric determinationReverse transcription-PCRBACKGROUND/Intracellular acidosisLoss of intracellularCell shrinkageHigher MCVFlow cytometryRT-PCRATP12APhosphatidylserine exposureUntreated cellsPotential roleMCVApoptosis
2010
Update on the Mechanisms of Gastric Acid Secretion
Kopic S, Geibel JP. Update on the Mechanisms of Gastric Acid Secretion. Current Gastroenterology Reports 2010, 12: 458-464. PMID: 20821079, DOI: 10.1007/s11894-010-0137-9.Peer-Reviewed Original Research
2009
Revisiting the parietal cell
Kopic S, Murek M, Geibel JP. Revisiting the parietal cell. American Journal Of Physiology - Cell Physiology 2009, 298: c1-c10. PMID: 19889964, DOI: 10.1152/ajpcell.00478.2009.Peer-Reviewed Original Research
2007
Stimulatory Pathways of the Calcium-Sensing Receptor on Acid Secretion in Freshly Isolated Human Gastric Glands
Remy C, Kirchhoff P, Hafner P, Busque S, Mueller M, Geibel J, Wagner C. Stimulatory Pathways of the Calcium-Sensing Receptor on Acid Secretion in Freshly Isolated Human Gastric Glands. Cellular Physiology And Biochemistry 2007, 19: 33-42. PMID: 17310098, DOI: 10.1159/000099190.Peer-Reviewed Original ResearchConceptsHeterotrimeric G proteinsHuman gastric glandsMAP kinaseGastric parietal cellsATPase activityG proteinsGastric acid secretionERK 1/2 MAP kinaseIndependent PKC isoformsStimulation of CaSRERK1/2 MAP kinasesAcid secretionProtein kinase CPKC-delta isoformParietal cellsStimulatory effectChelator of intracellularActivation of PKCGastric glandsHuman gastric parietal cellsPLC inhibitor U73122Sensitive heterotrimeric G proteinsPD 098059General inhibitorPKC isoformsPI3 Kinase Dependent Stimulation of Gastric Acid Secretion by Dexamethasone
Lang P, Schniepp R, Kirchhoff P, Socrates T, Sidani S, Geibel J. PI3 Kinase Dependent Stimulation of Gastric Acid Secretion by Dexamethasone. Cellular Physiology And Biochemistry 2007, 20: 527-534. PMID: 17762179, DOI: 10.1159/000107536.Peer-Reviewed Original ResearchMeSH KeywordsAndrostadienesAnimalsCimetidineDexamethasoneGastric AcidGastric MucosaH(+)-K(+)-Exchanging ATPaseMaleNitrobenzoatesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPotassium Channel BlockersProtein Kinase InhibitorsProton Pump InhibitorsRatsRats, Sprague-DawleyStaurosporineWortmanninConceptsGastric acid secretionProtein kinase inhibitor staurosporinePI3-kinase inhibitor wortmanninAcid secretionKinase inhibitor staurosporineKinase inhibitor wortmanninPI3-kinase pathwayPeptic ulcerDexamethasone effectApical Cl- channelsKinase pathwayInhibitor wortmanninInhibitor staurosporineExcessive gastric acid secretionProton extrusionATPase inhibitor omeprazoleCl- channel blockers NPPBCl- channelsChannel blocker NPPBDependent stimulationDexamethasone injectionVivo pretreatmentInhibitor omeprazoleParietal cellsSecretion
2006
An update on acid secretion
Geibel JP, Wagner C. An update on acid secretion. Reviews Of Physiology, Biochemistry And Pharmacology 2006, 156: 45-60. PMID: 16634146, DOI: 10.1007/s10254-005-0003-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumEnzyme ActivationGastric AcidGastric MucosaH(+)-K(+)-Exchanging ATPaseHumansModels, BiologicalParietal Cells, GastricReceptors, Calcium-SensingConceptsApical proteinsSecretory pathwayRegulatory mechanismsTransporter proteinsCalcium-sensing receptor activationParietal cellsApical surfaceCalcium-sensing receptorComplex processParietal cell canaliculiAcid productionProteinApical channelsReceptor activationInsertion of pumpsPathwayCellsActivationRecent candidateSecretion of acidSecretionReceptorsAcid secretionCatalytic cycleATPase
2005
An amino acid transporter involved in gastric acid secretion
Kirchhoff P, Dave MH, Remy C, Kosiek O, Busque SM, Dufner M, Geibel JP, Verrey F, Wagner CA. An amino acid transporter involved in gastric acid secretion. Pflügers Archiv - European Journal Of Physiology 2005, 451: 738-748. PMID: 16308696, DOI: 10.1007/s00424-005-1507-2.Peer-Reviewed Original ResearchConceptsAmino acid transportersAcid transportersAmino acidsHeteromeric amino acid transportersHeterodimeric amino acid transportersGastric acid secretionSystem L amino acid transportProtein-rich dietSystem L amino acid transportersSystem L aminoL amino acid transportersAmino acid transportParietal cellsAcid secretionPresence of glutamineNovel roleAcid transportVariety of stimuliIsolated rat gastric glandsTransportersATPase activityNon-metabolizable substratesSystem L.Serum amino acidsGlutamineThe calcium-sensing receptor acts as a modulator of gastric acid secretion in freshly isolated human gastric glands
Dufner MM, Kirchhoff P, Remy C, Hafner P, Müller M, Cheng SX, Tang LQ, Hebert SC, Geibel JP, Wagner CA. The calcium-sensing receptor acts as a modulator of gastric acid secretion in freshly isolated human gastric glands. AJP Gastrointestinal And Liver Physiology 2005, 289: g1084-g1090. PMID: 16109841, DOI: 10.1152/ajpgi.00571.2004.Peer-Reviewed Original ResearchConceptsHuman gastric glandsHistamine-induced activationGastric acid secretionGastric glandsExtracellular divalent cationsParietal cellsAcid secretionIsolated rat gastric glandsRelease of acetylcholineCalcium-sensing receptorEnterochromaffin-like cellsAbsence of histamineExtracellular divalent cation concentrationFunctional CaSRATPase activityVagus nerveNeuronal pathwaysRat gastric glandsFura-2Human patientsCaSRFluo-4Sustained increaseCalcium measurementsHistaminel-Type amino acids stimulate gastric acid secretion by activation of the calcium-sensing receptor in parietal cells
Busque SM, Kerstetter JE, Geibel JP, Insogna K. l-Type amino acids stimulate gastric acid secretion by activation of the calcium-sensing receptor in parietal cells. AJP Gastrointestinal And Liver Physiology 2005, 289: g664-g669. PMID: 15961860, DOI: 10.1152/ajpgi.00096.2005.Peer-Reviewed Original ResearchConceptsCalcium-sensing receptorAbsence of secretagoguesAcid secretionParietal cellsHistamine-2 receptorSystem L amino acid transportGastric acid secretionWhole stomach preparationDose-dependent increaseIndividual parietal cellsStomach preparationRat gastric glandsHEK-293 cellsCaSRL-amino acidsGastric glandsHormonal stimulationATPase activitySecretionPhysiological concentrationsReceptorsSecretagoguesAcid transportAmino acidsSecretory cellsRole of potassium in acid secretion
Geibel JP. Role of potassium in acid secretion. World Journal Of Gastroenterology 2005, 11: 5259-5265. PMID: 16149129, PMCID: PMC4622792, DOI: 10.3748/wjg.v11.i34.5259.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGastric AcidGastroesophageal RefluxH(+)-K(+)-Exchanging ATPaseHumansPotassiumPotassium Channel Blockers
2003
Demonstration of a functional apical sodium hydrogen exchanger in isolated rat gastric glands
Kirchhoff P, Wagner CA, Gaetzschmann F, Radebold K, Geibel JP. Demonstration of a functional apical sodium hydrogen exchanger in isolated rat gastric glands. AJP Gastrointestinal And Liver Physiology 2003, 285: g1242-g1248. PMID: 12907430, DOI: 10.1152/ajpgi.00165.2003.Peer-Reviewed Original ResearchAmilorideAnimalsBlotting, WesternCell MembraneEnzyme InhibitorsGastric MucosaGuanidinesH(+)-K(+)-Exchanging ATPaseHistamineHydrogen-Ion ConcentrationImmunohistochemistryIn Vitro TechniquesIntracellular MembranesIsoenzymesMethacrylatesOmeprazoleParietal Cells, GastricPerfusionRatsRats, Sprague-DawleySodium-Hydrogen Exchanger 3Sodium-Hydrogen Exchangers
2001
The Stomach Divalent Ion-sensing Receptor SCAR Is a Modulator of Gastric Acid Secretion*
Geibel J, Wagner C, Caroppo R, Qureshi I, Gloeckner J, Manuelidis L, Kirchhoff P, Radebold K. The Stomach Divalent Ion-sensing Receptor SCAR Is a Modulator of Gastric Acid Secretion*. Journal Of Biological Chemistry 2001, 276: 39549-39552. PMID: 11507103, DOI: 10.1074/jbc.m107315200.Peer-Reviewed Original ResearchConceptsDivalent cation receptorsTransport proteinsPlasma membrane transport proteinsMembrane transport proteinsGastric acid secretionGastric parietal cellsParietal cellsExact functionNovel pathwayGastric glandsReceptor familySecretory cellsAcid secretionCalcium-sensing receptorBasolateral membranePathwayEndocrine pathwaysExtracellular cationsProteinNew insightsATPaseRat gastric glandsRegulationCellsDivalent cations
2000
Ouabain-sensitive H,K-ATPase Functions as Na,K-ATPase in Apical Membranes of Rat Distal Colon*
Rajendran V, Sangan P, Geibel J, Binder H. Ouabain-sensitive H,K-ATPase Functions as Na,K-ATPase in Apical Membranes of Rat Distal Colon*. Journal Of Biological Chemistry 2000, 275: 13035-13040. PMID: 10777607, DOI: 10.1074/jbc.275.17.13035.Peer-Reviewed Original Research
1998
Role of Apical H-K Exchange and Basolateral K Channel in the Regulation of Intracellular pH in Rat Distal Colon Crypt Cells
Ikuma M, Binder H, Geibel J. Role of Apical H-K Exchange and Basolateral K Channel in the Regulation of Intracellular pH in Rat Distal Colon Crypt Cells. The Journal Of Membrane Biology 1998, 166: 205-212. PMID: 9843594, DOI: 10.1007/s002329900462.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiportersBariumCell PolarityColonEpithelial CellsH(+)-K(+)-Exchanging ATPaseHomeostasisHydrogen-Ion ConcentrationIntestinal MucosaIntracellular FluidIon TransportMaleOuabainPotassiumPotassium ChannelsPotassium-Hydrogen AntiportersRatsRats, Sprague-DawleySodium-Potassium-Exchanging ATPaseSorting of P-type ATPases in polarized epithelial cells.
Dunbar LA, Courtois-Coutry N, Roush DL, Muth TR, Gottardi CJ, Rajendran V, Geibel J, Kashgarian M, Caplan MJ. Sorting of P-type ATPases in polarized epithelial cells. Acta Physiologica Scandinavica. Supplementum 1998, 643: 289-95. PMID: 9789572.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell PolarityEpithelial CellsH(+)-K(+)-Exchanging ATPaseSodium-Potassium-Exchanging ATPaseConceptsApical localizationK-ATPaseMost epithelial cell typesTyrosine-based signalsP-type familyP-type ATPasesEpithelial cellsCritical tyrosine residuesApical plasma membraneFourth transmembrane domainBeta-subunit sequencesApical cell surfaceEpithelial cell typesSorting signalsTransmembrane domainCytoplasmic tailSequence domainsPlasma membraneHomologous membersTyrosine residuesParietal cellsStorage compartmentCell typesCell surfaceBasolateral surfaceDifferential localization of colonic H+-K+-ATPase isoforms in surface and crypt cells
Rajendran V, Singh S, Geibel J, Binder H. Differential localization of colonic H+-K+-ATPase isoforms in surface and crypt cells. American Journal Of Physiology 1998, 274: g424-g429. PMID: 9486199, DOI: 10.1152/ajpgi.1998.274.2.g424.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBariumColonH(+)-K(+)-Exchanging ATPaseHydrogen-Ion ConcentrationIsoenzymesMaleOuabainPotassiumRatsRats, Sprague-DawleySurface PropertiesConceptsCrypt cellsColonic cryptsRat distal colonSurface cellsSurface epithelial cellsATPase isoformsOuabain-insensitive componentApical membraneDistal colonAcid loadDependent intracellularEpithelial cellsATPase activityCryptsDifferential localizationOuabainATPase alpha subunitCellsAlpha subunitCompelling evidenceIsoformsColonActivityRecovery
1997
Sorting of Ion Pumps in Polarized Epithelial Cells.a
DUNBAR L, ROUSH D, COURTOIS‐COUTRY N, MUTH T, GOTTARDI CJ, RAJENDRAN V, GEIBEL J, KASHGARIAN M, CAPLAN M. Sorting of Ion Pumps in Polarized Epithelial Cells.a. Annals Of The New York Academy Of Sciences 1997, 834: 514-523. PMID: 9405853, DOI: 10.1111/j.1749-6632.1997.tb52309.x.Peer-Reviewed Original ResearchAmino Acid SequenceCell MembraneCell PolarityConserved SequenceEpithelial CellsH(+)-K(+)-Exchanging ATPaseHumansMacromolecular SubstancesModels, MolecularMolecular Sequence DataProtein Structure, SecondaryReceptors, TransferrinRecombinant Fusion ProteinsSequence AlignmentSequence Homology, Amino AcidSodium-Potassium-Exchanging ATPase