2014
The Putative Role of the Non-Gastric H+/K+-ATPase ATP12A (ATP1AL1) as Anti-Apoptotic Ion Transporter: Effect of the H+/K+ ATPase Inhibitor SCH28080 on Butyrate-Stimulated Myelomonocytic HL-60 Cells
Jakab M, Hofer S, Ravasio A, Huber F, Schmidt S, Hitzl W, Geibel JP, Fürst J, Ritter M. The Putative Role of the Non-Gastric H+/K+-ATPase ATP12A (ATP1AL1) as Anti-Apoptotic Ion Transporter: Effect of the H+/K+ ATPase Inhibitor SCH28080 on Butyrate-Stimulated Myelomonocytic HL-60 Cells. Cellular Physiology And Biochemistry 2014, 34: 1507-1526. PMID: 25322912, DOI: 10.1159/000366355.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisButyratesCell Line, TumorCell MembraneH(+)-K(+)-Exchanging ATPaseHL-60 CellsHumansImidazolesIon TransportConceptsIntracellular acid loadHL-60 cellsAcute intracellular acid loadAcid loadMCV measurementsOsmotic cell shrinkageReal-time reverse transcription PCREarly apoptosisChanges of intracellularCD86 expressionFlow cytometric determinationReverse transcription-PCRBACKGROUND/Intracellular acidosisLoss of intracellularCell shrinkageHigher MCVFlow cytometryRT-PCRATP12APhosphatidylserine exposureUntreated cellsPotential roleMCVApoptosis
2012
Effect of the AMP-Kinase Modulators AICAR, Metformin and Compound C on Insulin Secretion of INS-1E Rat Insulinoma Cells under Standard Cell Culture Conditions
Langelueddecke C, Jakab M, Ketterl N, Lehner L, Hufnagl C, Schmidt S, Geibel JP, Fuerst J, Ritter M. Effect of the AMP-Kinase Modulators AICAR, Metformin and Compound C on Insulin Secretion of INS-1E Rat Insulinoma Cells under Standard Cell Culture Conditions. Cellular Physiology And Biochemistry 2012, 29: 75-86. PMID: 22415077, DOI: 10.1159/000337589.Peer-Reviewed Original ResearchConceptsAMP-dependent protein kinaseCompound CStandard cell culture conditionsCell culture conditionsINS-1E rat insulinoma cellsAMPK phosphorylationAMPK inhibitor compound CCulture conditionsCell membrane potentialAMPK-independent mechanismCell metabolic stateInhibitor compound CINS-1E cellsMetabolic stateMembrane potentialStimulus-secretion couplingBasal AMPK phosphorylationΒ-cell survivalCell cycle arrestProtein kinaseΒ-cell line INS-1E.Nutrient uptakeAMPK activatorCell cycle distributionInsulin secretion
2009
The Phytostilbene Resveratrol Induces Apoptosis in INS-1E Rat Insulinoma Cells
Bortolotti C, Kunit T, Moder A, Hufnagl C, Schmidt S, Hartl A, Langelueddecke C, Fürst J, Geibel J, Ritter M, Jakab M. The Phytostilbene Resveratrol Induces Apoptosis in INS-1E Rat Insulinoma Cells. Cellular Physiology And Biochemistry 2009, 23: 245-254. PMID: 19471092, DOI: 10.1159/000218171.Peer-Reviewed Original ResearchConceptsINS-1E rat insulinoma cellsINS-1E cellsApoptotic volume decreaseRat insulinoma cellsAkt phosphorylationInsulinoma cellsCell proliferationProapoptotic effectsInhibitor compound CBasal Akt phosphorylationExposure of phosphatidylserineInduction of apoptosisG0/G1 phaseCaspase activationPlasma membraneOuter leafletCompound CMuM resveratrolG1 phaseCrystal violet stainingApoptotic cellsCell granularityAddition of insulinPhosphorylationMajor target
2006
Hypertonic Saline Attenuates Colonic Tumor Cell Metastatic Potential by Activating Transmembrane Sodium Conductance
Shields CJ, Winter DC, Geibel JP, O’Sullivan G, Wang JH, Redmond HP. Hypertonic Saline Attenuates Colonic Tumor Cell Metastatic Potential by Activating Transmembrane Sodium Conductance. The Journal Of Membrane Biology 2006, 211: 35-42. PMID: 16988862, DOI: 10.1007/s00232-006-0011-8.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TumorCell MembraneColonic NeoplasmsHumansIntegrin beta1Neoplasm MetastasisSaline Solution, HypertonicSodiumConceptsHypertonic salineSodium-hydrogen exchangeIntegrin expressionIntracellular sodiumMetastatic potentialHuman colonic tumor cellsAdhesion molecule expressionSodium concentrationColonic tumor cellsTumor cell metastatic potentialIntracellular pHCell metastatic potentialCytosolic sodium concentrationMolecule expressionΒ1 integrin expressionHyperosmolar solutionsConfocal laser microscopic imagingEffect of hypertonicityTumor cellsInhibitory effectSodium responseHypertonic exposureCellular alkalinizationTumor cell-endothelial interactionsSodium conductance