2015
Nanoparticles that deliver triplex-forming peptide nucleic acid molecules correct F508del CFTR in airway epithelium
McNeer NA, Anandalingam K, Fields RJ, Caputo C, Kopic S, Gupta A, Quijano E, Polikoff L, Kong Y, Bahal R, Geibel JP, Glazer PM, Saltzman WM, Egan ME. Nanoparticles that deliver triplex-forming peptide nucleic acid molecules correct F508del CFTR in airway epithelium. Nature Communications 2015, 6: 6952. PMID: 25914116, PMCID: PMC4480796, DOI: 10.1038/ncomms7952.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineChloridesCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorDNA-Binding ProteinsGenetic TherapyHigh-Throughput Nucleotide SequencingHumansLactic AcidMice, Inbred C57BLNanoparticlesPeptide Nucleic AcidsPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerPolymersRespiratory MucosaConceptsFacile genome engineeringVivo gene deliveryBiodegradable polymer nanoparticlesTransient gene expressionNanoparticle systemsGene deliveryPolymer nanoparticlesGene correctionGenome engineeringNanoparticlesOff-target effectsPeptide nucleic acidLethal genetic disorderNucleic acidsDonor DNATarget effectsIntranasal deliveryDeliveryCystic fibrosisEngineeringOligonucleotideChloride effluxHuman cellsAirway epitheliumLung tissue
2007
Characteristics of rat downregulated in adenoma (rDRA) expressed in HEK 293 cells
Barmeyer C, Ye JH, Sidani S, Geibel J, Binder HJ, Rajendran VM. Characteristics of rat downregulated in adenoma (rDRA) expressed in HEK 293 cells. Pflügers Archiv - European Journal Of Physiology 2007, 454: 441-450. PMID: 17492310, DOI: 10.1007/s00424-007-0213-7.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmino Acid SequenceAnimalsAntiportersBase SequenceBicarbonatesCell LineChloridesCloning, MolecularColonDNA PrimersFatty Acids, VolatileHumansHydrogen-Ion ConcentrationIon TransportMolecular Sequence DataRatsRecombinant ProteinsSequence Homology, Amino AcidSulfate TransportersTransfectionConceptsRat distal colonDistal colonHEK-293 cellsExtracellular Cl- concentrationCrypt cellsMRNA expressionColonRatsDigestive tractCharacteristics of ratsAdenomasHigh rateDIDSCl- influxSurface cellsIntracellular acidApical membrane vesiclesCellsDisulfonic acidApical membraneCl- uptakePrior observationsUptakeLungJejunum
2003
Intracellular pH Activates Membrane-Bound Na+/H+ Exchanger and Vacuolar H+-ATPase in Human Embryonic Kidney (HEK) Cells
Lang K, Wagner C, Haddad G, Burnekova O, Geibel J. Intracellular pH Activates Membrane-Bound Na+/H+ Exchanger and Vacuolar H+-ATPase in Human Embryonic Kidney (HEK) Cells. Cellular Physiology And Biochemistry 2003, 13: 257-262. PMID: 14586169, DOI: 10.1159/000074540.Peer-Reviewed Original ResearchConceptsMicroM EIPAIntracellular acidificationFree bath solutionProximal tubule cellsInfluence of intracellularWestern blot analysisTime-dependent mannerTubule cellsHuman embryonic kidney cellsHEK-293 cellsExtruding mechanismHuman embryonic kidney cell line HEK-293NHE3 proteinEmbryonic kidney cellsCell line HEK-293Cytosolic pHKidney cellsBlot analysisAbsence of bicarbonateHEK293 cellsBath solutionEIPAPresent studyActivationCells
2002
Expression of calcium-sensing receptor in rat colonic epithelium: evidence for modulation of fluid secretion
Cheng SX, Okuda M, Hall AE, Geibel JP, Hebert SC. Expression of calcium-sensing receptor in rat colonic epithelium: evidence for modulation of fluid secretion. AJP Gastrointestinal And Liver Physiology 2002, 283: g240-g250. PMID: 12065312, DOI: 10.1152/ajpgi.00500.2001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportBody FluidsCalciumCell LineCell MembraneColonHumansIn Vitro TechniquesIntestinal MucosaIntracellular MembranesMalePhosphatidylinositol Diacylglycerol-LyaseRatsRats, Sprague-DawleyReceptors, Calcium-SensingReceptors, Cell SurfaceTranscription, GeneticType C PhospholipasesConceptsCalcium-sensing receptorCrypt cellsFluid secretionNet fluid secretionIntestinal fluid transportRat colonic epitheliumCaSR agonistsIntracellular calciumExtracellular calciumColonic epitheliumDiarrheal diseaseCaSR transcriptsBath perfusateStimulatory effectBody cellsSecretionReceptorsCryptsElevated cAMPBasolateral surfaceCellsDivalent mineralsCalciumCaSRAgonists
2001
Cloning and Expression of a Chloride-dependent Na+-H+ Exchanger*
Sangan P, Rajendran VM, Geibel JP, Binder HJ. Cloning and Expression of a Chloride-dependent Na+-H+ Exchanger*. Journal Of Biological Chemistry 2001, 277: 9668-9675. PMID: 11773056, DOI: 10.1074/jbc.m110852200.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAmino Acid SequenceAnimalsBlotting, NorthernCell LineChlorineCloning, MolecularColonDiureticsDNA, ComplementaryFibroblastsHydrogen-Ion ConcentrationIn Situ HybridizationMaleMolecular Sequence DataPhosphorylationProtein Structure, TertiaryRatsRats, Sprague-DawleyRNA, MessengerSequence Homology, Amino AcidSodiumSodium-Hydrogen Exchanger 1Sodium-Hydrogen ExchangersTime FactorsTissue DistributionConceptsRat distal colonDistal colonAmino acidsCrypt cellsExchange isoformsDietary sodium depletionColonic crypt cellsFull-length cDNACl- channel blockerColonic epithelial cellsN-terminal regionNorthern blot analysisSodium depletionChannel blockersNovel C-terminusPutative transmembranePS120 fibroblastsAcid loadDependent intracellularC-terminusNHE-1Epithelial cellsIsoform inhibitorAmiloride analoguesMultiple tissuesEffects of the Serine/Threonine Kinase SGK1 on the Epithelial Na+ Channel (ENaC) and CFTR: Implications for Cystic Fibrosis
Wagner C, Ott M, Klingel K, Beck S, Melzig J, Friedrich B, Wild K, Bröer S, Moschen I, Albers A, Waldegger S, Tümmler B, Egan M, Geibel J, Kandolf R, Lang F. Effects of the Serine/Threonine Kinase SGK1 on the Epithelial Na+ Channel (ENaC) and CFTR: Implications for Cystic Fibrosis. Cellular Physiology And Biochemistry 2001, 11: 209-218. PMID: 11509829, DOI: 10.1159/000051935.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAmino Acid SubstitutionAnimalsBronchiCell LineCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorEpithelial CellsEpithelial Sodium ChannelsHumansIn Situ HybridizationLungMacrophages, AlveolarMutationOocytesPatch-Clamp TechniquesProtein Serine-Threonine KinasesPulmonary AlveoliRNA, ComplementaryRNA, MessengerSodiumSodium ChannelsXenopus laevisConceptsSerine/threonine kinase SGK1Lung tissueCystic fibrosisCF patientsKinase SGK1CF lung tissueXenopus oocytesLoss of CFTRLung epithelial cell lineCoexpression of CFTREffect of SGK1Pathophysiological factorsEpithelial cell lineRespiratory epitheliumLung phenotypeVariety of stimuliCl(-) secretionSGK1 expressionInhibitor amilorideInhibitory effectEpithelial cellsEnhanced expressionChannel ENaC.CFTR mutationsChannel activityK+-induced HSP-72 expression is mediated via rapid Ca2+ influx in renal epithelial cells
Eickelberg O, Geibel J, Seebach F, Giebisch G, Kashgarian M. K+-induced HSP-72 expression is mediated via rapid Ca2+ influx in renal epithelial cells. American Journal Of Physiology. Renal Physiology 2001, 281: f280-f287. PMID: 11457719, DOI: 10.1152/ajprenal.2001.281.2.f280.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium Channel BlockersCell LineDiltiazemEgtazic AcidEnzyme InhibitorsEpithelial CellsGallic AcidGenes, ReporterHeat-Shock ProteinsHSP72 Heat-Shock ProteinsImmunoblottingKidney Tubules, ProximalMicroscopy, ConfocalPotassiumPromoter Regions, GeneticRecombinant Fusion ProteinsSodiumSwineThapsigarginUrotheliumConceptsHSP 72 expressionPromoter activityHSP 72Protein expressionProtective cellular responseLuciferase reporter geneHSP-25Heat shock protein expressionRenal epithelial cellsTranscriptional inductionShock protein expressionIonic stressReporter geneHSP-90 levelsHSC 73Cellular responsesChannel blocker diltiazemIntracellular lumenWestern blot analysisChelator EGTA-AMPathophysiological stimuliBlot analysisConfocal microscopyProtein levelsExtracellular space
1999
Continuous detection of extracellular ATP on living cells by using atomic force microscopy
Schneider S, Egan M, Jena B, Guggino W, Oberleithner H, Geibel J. Continuous detection of extracellular ATP on living cells by using atomic force microscopy. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 12180-12185. PMID: 10518596, PMCID: PMC18432, DOI: 10.1073/pnas.96.21.12180.Peer-Reviewed Original ResearchConceptsAtomic force microscopyForce microscopyLiving cellsImportant compoundsSurface of cellsATP concentrationSurface microenvironmentScanning tipExtracellular ATPCell linesMicroscopyPhysiological conditionsPowerful techniqueCellsContinuous detectionBiosensorSurfaceCompoundsActive tipATPConcentrationFuture developmentSoftnessDetectionMicroenvironment
1997
Differential activities of H+extrusion systems in MDCK cells due to extracellular osmolality and pH
Feifel E, Krall M, Geibel J, Pfaller W. Differential activities of H+extrusion systems in MDCK cells due to extracellular osmolality and pH. American Journal Of Physiology 1997, 273: f499-f506. PMID: 9362327, DOI: 10.1152/ajprenal.1997.273.4.f499.Peer-Reviewed Original ResearchLocalization of amiloride-sensitive sodium channels in A6 cells by atomic force microscopy
Smith P, Bradford A, Schneider S, Benos D, Geibel J. Localization of amiloride-sensitive sodium channels in A6 cells by atomic force microscopy. American Journal Of Physiology 1997, 272: c1295-c1298. PMID: 9142855, DOI: 10.1152/ajpcell.1997.272.4.c1295.Peer-Reviewed Original ResearchConceptsAtomic force microscopyHigh-resolution imagingRenal epithelial cellsForce microscopyA6 cellsApical microvilliCell surface distributionBovine renal medullaEpithelial cellsA6 renal epithelial cellsAmiloride-sensitive sodium channelsEpithelial ion channelsColloidal gold particlesApical distributionIon channelsChannel complexGold particlesSodium channelsCellsMicrovilliNonimmune immunoglobulin GUnlabeled cellsMicroscopySurface distributionParticles
1995
Visualizing life on biomembranes by atomic force microscopy
Oberleithner H, Brinckmann E, Giebisch G, Geibel J. Visualizing life on biomembranes by atomic force microscopy. Kidney International 1995, 48: 923-929. PMID: 8569101, DOI: 10.1038/ki.1995.373.Peer-Reviewed Original ResearchConceptsAtomic force microscopeNear-field microscopeSoft biological samplesAtomic force microscopyPlasma membrane turnoverForce microscopeForce microscopyPore complexRenal epithelial cellsAtomic resolutionNuclear envelopeAFM techniquesExperimental biologistsMembrane turnoverMolecular structureKidney cellsEpithelial cellsBiological samplesMicroscopeCellsBiomembranesRepresentative examplesRecent dataBiologistsMicroscopy
1994
Living Renal Epithelial Cells Imaged by Atomic Force Microscopy
Oberleithner H, Schwab A, Wang W, Giebisch G, Hume F, Geibel J. Living Renal Epithelial Cells Imaged by Atomic Force Microscopy. Nephron 1994, 66: 8-13. PMID: 8107959, DOI: 10.1159/000187759.Peer-Reviewed Original Research
1993
Imaging the lamellipodium of migrating epithelial cells in vivo by atomic force microscopy
Oberleithner H, Giebisch G, Geibel J. Imaging the lamellipodium of migrating epithelial cells in vivo by atomic force microscopy. Pflügers Archiv - European Journal Of Physiology 1993, 425: 506-510. PMID: 8134267, DOI: 10.1007/bf00374878.Peer-Reviewed Original ResearchConceptsPlasma membraneCell locomotionNew plasma membraneCell surfacePlasma membrane potentialCanine kidney cell linePlasma membrane integrityMadin-Darby canine kidney (MDCK) cell lineDynamic physiological processesKidney cell lineAlkaline stressInvagination processCytoskeletal elementsMDCK-F cellsPhysiological processesMembrane integrityMembrane turnoverCell linesKidney cellsMembrane potentialEpithelial cellsTurnover processCellsMembranePotential role