2006
The KCNE2 Potassium Channel Ancillary Subunit Is Essential for Gastric Acid Secretion*
Roepke TK, Anantharam A, Kirchhoff P, Busque SM, Young JB, Geibel JP, Lerner DJ, Abbott GW. The KCNE2 Potassium Channel Ancillary Subunit Is Essential for Gastric Acid Secretion*. Journal Of Biological Chemistry 2006, 281: 23740-23747. PMID: 16754665, DOI: 10.1074/jbc.m604155200.Peer-Reviewed Original ResearchConceptsAncillary subunitsKCNE gene familyGastric acid secretionPotassium channel alpha subunitFirst genetic evidenceSecretory cellsProton secretionChannel alpha subunitGene familyKCNE familyParietal cell morphologyAcid secretionGenetic evidenceAlpha subunitHuman cardiac arrhythmiasParietal cellsChannel complexSubunitsCell morphologyKCNE2Normal gastrointestinal functionGenesKCNQ1Gene-dose effectCells
2005
Insulin Receptor (IR) and Glucose Transporter 2 (GLUT2) Proteins Form a Complex on the Rat Hepatocyte Membrane
Eisenberg M, Maker A, Slezak L, Nathan J, Sritharan K, Jena B, Geibel J, Andersen D. Insulin Receptor (IR) and Glucose Transporter 2 (GLUT2) Proteins Form a Complex on the Rat Hepatocyte Membrane. Cellular Physiology And Biochemistry 2005, 15: 051-058. PMID: 15665515, DOI: 10.1159/000083638.Peer-Reviewed Original ResearchConceptsGlucose transporter 2Insulin receptorPlasma membranePlasma membrane insulin receptorsFluorescence resonance energy transfer studiesMembrane insulin receptorsHepatic glucose transportersGlucose transporterHepatocyte plasma membraneHepatic glucose regulationInsulin regulationGlucose transportBidirectional glucose transportRat hepatocyte membranesConfocal microscopyTransporter 2MembraneEnergy transfer studiesRegulationTexas RedComplexesEndosomesCytosolProteinTransporters
2002
Calcium-pump inhibitors induce functional surface expression of ΔF508-CFTR protein in cystic fibrosis epithelial cells
Egan ME, Glöckner-Pagel J, Ambrose C, Cahill PA, Pappoe L, Balamuth N, Cho E, Canny S, Wagner CA, Geibel J, Caplan MJ. Calcium-pump inhibitors induce functional surface expression of ΔF508-CFTR protein in cystic fibrosis epithelial cells. Nature Medicine 2002, 8: 485-492. PMID: 11984593, DOI: 10.1038/nm0502-485.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumCalcium pump inhibitorΔF508-CFTR proteinCystic fibrosis epithelial cellsCystic fibrosis transmembrane conductance regulator (CFTR) proteinCystic fibrosis cell lineFunctional surface expressionSurface expressionChaperone activityChaperone proteinsRegulator proteinPlasma membraneCystic fibrosis defectCell surfaceProteinCell linesPotential targetOptimal activityInhibitor thapsigarginEpithelial cellsExpressionCommon mutationsInhibitorsMouse modelReticulum
1999
Expression of an extracellular calcium-sensing receptor in rat stomach
Cheng* ‡, Qureshi§ I, Chattopadhyay‡ N, Qureshi§ A, Butters‡ R, Hall∥ A, Cima* R, Rogers∥ K, Hebert∥ S, Geibel§ J, Brown‡ E, Soybel* D, ¶NPS Pharmaceuticals I. Expression of an extracellular calcium-sensing receptor in rat stomach. Gastroenterology 1999, 116: 118-126. PMID: 9869609, DOI: 10.1016/s0016-5085(99)70235-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, NorthernCalciumCells, CulturedDNA, ComplementaryExtracellular SpaceGastric MucosaImmunohistochemistryMolecular Sequence DataMuscle, SmoothParietal Cells, GastricRatsRats, Sprague-DawleyReceptors, Calcium-SensingReceptors, Cell SurfaceReverse Transcriptase Polymerase Chain ReactionRNAStomachTranscription, GeneticConceptsGastric glandsExtracellular calcium-sensing receptorEpithelial cellsCalcium-sensing receptorReverse transcription-polymerase chain reactionGastric surface cellsMammalian gastric mucosaExpression of CARMyenteric neuronsSubmucosal plexusSurface cellsGastric functionPolymerase chain reactionNerve regionRat stomachGastric mucosaGlandular stomachExtracellular Ca2Secretory functionRat parathyroidSystemic Ca2AbstractTextLevels of Ca2CAR expressionStomach
1998
Atomic Force Microscopy on Living Cells: Aldosterone–Induced Localized Cell Swelling
Schneider SW, Pagel P, Storck J, Yano Y, Sumpio BE, Geibel JP, Oberleithner H. Atomic Force Microscopy on Living Cells: Aldosterone–Induced Localized Cell Swelling. Kidney & Blood Pressure Research 1998, 21: 256-258. PMID: 9762848, DOI: 10.1159/000025869.Peer-Reviewed Original Research
1997
RAPID ALDOSTERONE‐INDUCED CELL VOLUME INCREASE OF ENDOTHELIAL CELLS MEASURED BY THE ATOMIC FORCE MICROSCOPE
SCHNEIDER S, YANO Y, SUMPIO B, JENA B, GEIBEL J, GEKLE M, OBERLEITHNER H. RAPID ALDOSTERONE‐INDUCED CELL VOLUME INCREASE OF ENDOTHELIAL CELLS MEASURED BY THE ATOMIC FORCE MICROSCOPE. Cell Biology International 1997, 21: 759-768. PMID: 9768474, DOI: 10.1006/cbir.1997.0220.Peer-Reviewed Original ResearchMeSH KeywordsAldosteroneAnimalsCattleCell SizeCells, CulturedEndothelium, VascularHumansMicroscopy, Atomic ForceConceptsEndothelial cellsAddition of aldosteroneArterial blood pressureNon-genomic effectsCultured endothelial cellsBlood pressureMineralocorticoid hormonesHormone exposureAldosteroneCell volumeHuman leukocytesIntracellular electrolytesTransient swellingTriphosphate productionIntracellular pHLeukocytesVolume increaseBovine aortaContinuous presenceCellsPlasma membraneCyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells
Yano Y, Geibel J, Sumpio B. Cyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells. Journal Of Cellular Biochemistry 1997, 64: 505-513. PMID: 9057108, DOI: 10.1002/(sici)1097-4644(19970301)64:3<505::aid-jcb17>3.0.co;2-e.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsBeta 1 integrinUmbilical vein endothelial cellsVein endothelial cellsEndothelial cellsAlpha 5Beta 1Alpha 5 beta 1Alpha 2 beta 1 integrinH exposureBeta 3 integrinAlpha 2 integrinCycles/minEndothelial cell morphologyVariety of stimuliIntegrin alpha 5Tyrosine phosphorylationVitronectin receptorAlpha 2Redistribution of alphaEC subjectsBeta integrins
1996
Tyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain
Yano Y, Geibel J, Sumpio BE. Tyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain. American Journal Of Physiology 1996, 271: c635-c649. PMID: 8770005, DOI: 10.1152/ajpcell.1996.271.2.c635.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell Adhesion MoleculesCell MovementCells, CulturedCytoskeletal ProteinsEndothelium, VascularFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGenisteinIsoflavonesNitrilesPaxillinPhosphoproteinsPhosphorylationPhosphotyrosineProtein-Tyrosine KinasesStress, MechanicalTissue DistributionTyrosineTyrphostins
1994
The Mastomys Gastric Carcinoid: Aspects of Enterochromaff in-Like Cell Function
Modlin I, Lawton G, Tang L, Geibel J, Abraham R, Darr U. The Mastomys Gastric Carcinoid: Aspects of Enterochromaff in-Like Cell Function. Digestion 1994, 55: 31-37. PMID: 7535269, DOI: 10.1159/000201199.Peer-Reviewed Original ResearchConceptsECL cellsHistamine-1 receptor antagonistsGastric carcinoid tumorsCalcium-dependent mechanismBeta-adrenergic stimulationCarcinoid tumorsGastric carcinoidsReceptor antagonistHistamine secretionTumor growthLike cell functionCell functionGenetic propensitySecretionRegulation of growthMastomys rodentsCarcinoidsCellsMuscarinicPharmacotherapySomatostatinAntagonistTumorsHistamine
1993
Endothelin increases [Ca2+]i in M-1 mouse cortical collecting duct cells by a dual mechanism
Korbmacher C, Boulpaep E, Giebisch G, Geibel J. Endothelin increases [Ca2+]i in M-1 mouse cortical collecting duct cells by a dual mechanism. American Journal Of Physiology 1993, 265: c349-c357. PMID: 8368264, DOI: 10.1152/ajpcell.1993.265.2.c349.Peer-Reviewed Original ResearchConceptsEndothelin-1Intracellular storesDuct cellsNifedipine-sensitive Ca2Intracellular calcium concentrationParticipation of Ca2Endothelin increaseFura-2Extracellular calciumExtracellular Ca2Arginine vasopressinIntracellular Ca2Calcium concentrationInitial Ca2Fluorescent imaging systemInitial exposurePlateau phaseMiceEntry mechanismDual mechanismCa2Second Ca2CellsNifedipineVasopressinMechanism of activation of K++ channels by minoxidil-sulfate in Madin-Darby canine kidney cells
Schwab A, Geibel J, Wang W, Oberleithner H, Giebisch G. Mechanism of activation of K++ channels by minoxidil-sulfate in Madin-Darby canine kidney cells. The Journal Of Membrane Biology 1993, 132: 125-136. PMID: 8496944, DOI: 10.1007/bf00239002.Peer-Reviewed Original Research