2017
Acute Effects of Vitamin C Exposure On Colonic Crypts: Direct Modulation of pH Regulation
Aldajani MM, Vanicek CN, Alhazzaa N, Lysyy T, Agarwal R, Geibel JP. Acute Effects of Vitamin C Exposure On Colonic Crypts: Direct Modulation of pH Regulation. Cellular Physiology And Biochemistry 2017, 44: 377-387. PMID: 29132138, DOI: 10.1159/000484914.Peer-Reviewed Original ResearchConceptsVitamin CColonic cryptsAcute effectsDependent intracellular pH recoveryTumor formationIntracellular pH recoveryNew therapeutic agentsMM vitamin CColorectal cancerDistal colonLeading causeTranscellular passive diffusionBasolateral membraneAcute additionNormal colonic cryptsC exposureNutraceutical agentTherapeutic agentsPH recoveryIntracellular pH changesCancer cellsVitamin C.CryptsLow intracellularTight junctions
2013
Activation of AMPK Inhibits Cholera Toxin Stimulated Chloride Secretion in Human and Murine Intestine
Rogers AC, Huetter L, Hoekstra N, Collins D, Collaco A, Baird AW, Winter DC, Ameen N, Geibel JP, Kopic S. Activation of AMPK Inhibits Cholera Toxin Stimulated Chloride Secretion in Human and Murine Intestine. PLOS ONE 2013, 8: e69050. PMID: 23935921, PMCID: PMC3728293, DOI: 10.1371/journal.pone.0069050.Peer-Reviewed Original ResearchConceptsIntestinal chloride secretionCholera toxinChloride secretionPharmacological activationFluid accumulationColonic cryptsSupplemental treatment strategyAcute diarrheal illnessChloride effluxCystic fibrosis transmembrane conductance regulatorExcised intestinal segmentsRat colonic cryptsMurine colonic mucosaMucosal sheetsDiarrheal illnessIntestinal loopsEnterotoxigenic diarrheaColonic mucosaTreatment strategiesIntestinal segmentsUssing chambersAMPK activator AICARMurine intestineTissue levelsMetforminThe calcium sensing receptor modulates fluid reabsorption and acid secretion in the proximal tubule
Capasso G, Geibel PJ, Damiano S, Jaeger P, Richards WG, Geibel JP. The calcium sensing receptor modulates fluid reabsorption and acid secretion in the proximal tubule. Kidney International 2013, 84: 277-284. PMID: 23615500, DOI: 10.1038/ki.2013.137.Peer-Reviewed Original ResearchMeSH KeywordsAcid-Base EquilibriumAnimalsCalcimimetic AgentsCalciumHydrogen-Ion ConcentrationIn Vitro TechniquesKidney Tubules, ProximalMaleMiceMice, KnockoutNuclear ProteinsPerfusionPuncturesRatsRats, Sprague-DawleyReceptors, Calcium-SensingReceptors, G-Protein-CoupledSodium-Hydrogen ExchangersTranscription FactorsConceptsProximal tubulesLuminal calcium concentrationFluid reabsorptionAcid secretionKnockout miceCalcium ion concentrationCalcium concentrationRole of CaSRActivation of CaSRCalcium sensing receptorCalcium-sensing receptorMouse proximal tubulesProton secretionSodium-hydrogen exchangerCalcimimetic agentBicarbonate absorptionSensing receptorCaSRFluid absorptionSecretionReabsorptionMiceCalcimimeticsTubulesLuminal membrane
2011
The flavonone naringenin inhibits chloride secretion in isolated colonic epithelia
Collins D, Kopic S, Geibel JP, Hogan AM, Medani M, Baird AW, Winter DC. The flavonone naringenin inhibits chloride secretion in isolated colonic epithelia. European Journal Of Pharmacology 2011, 668: 271-277. PMID: 21762688, DOI: 10.1016/j.ejphar.2011.06.052.Peer-Reviewed Original ResearchConceptsHuman colonic mucosaChloride secretionAntisecretory actionColonic mucosaCo-transporter inhibitorsEffect of naringeninIntracellular cAMP generationColonic cell lineAntisecretory effectColonic functionColorectal cancerProtective effectUssing chambersColonic epitheliumSecretory functionCAMP generationInhibitory actionBasolateral NKCC1Human colonRatsSecretionIntracellular acidificationConflicting resultsCell linesMucosa
2007
SLC26A7 Can function as a chloride-loading mechanism in parietal cells
Kosiek O, Busque SM, Föller M, Shcheynikov N, Kirchhoff P, Bleich M, Muallem S, Geibel JP. SLC26A7 Can function as a chloride-loading mechanism in parietal cells. Pflügers Archiv - European Journal Of Physiology 2007, 454: 989-998. PMID: 17404755, DOI: 10.1007/s00424-007-0254-y.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsAntiportersChloride-Bicarbonate AntiportersChloridesFluorescent Antibody TechniqueGastric AcidGastric MucosaImage Processing, Computer-AssistedIn Vitro TechniquesMaleOocytesParietal Cells, GastricPatch-Clamp TechniquesRatsRats, Sprague-DawleySodiumSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 2Sulfate TransportersXenopusConceptsParietal cellsXenopus laevis oocyte expression systemRat parietal cellsExpression of SLC26A7Rat gastric glandsAcid secretionOocyte expression systemNKCC inhibitorWestern blotHistamine stimulationPharmacological inhibitorsAcidic pHiGastric glandsDIDS concentrationExchanger AE2Subsequent readditionTransporter activityDisulfonic acidInhibitorsCellsSLC26A7InfluxImmunohistochemistrySecretagoguesUptake system
2006
Characteristics of the K+-competitive H+,K+-ATPase inhibitor AZD0865 in isolated rat gastric glands
Kirchhoff P, Andersson K, Socrates T, Sidani S, Kosiek O, Geibel J. Characteristics of the K+-competitive H+,K+-ATPase inhibitor AZD0865 in isolated rat gastric glands. AJP Gastrointestinal And Liver Physiology 2006, 291: g838-g843. PMID: 16798725, DOI: 10.1152/ajpgi.00120.2006.Peer-Reviewed Original ResearchConceptsGastric acid secretionAcid secretionRat gastric glandsPotassium-competitive acid blockerGastric glandsPeptic ulcer diseaseProton pump inhibitor omeprazoleAcid-related diseasesCompetitive acid blockerPresence of omeprazoleLow-dose inhibitorsFree perfusateUlcer diseasePharmacological treatmentAZD0865High success rateAcid blockerLow doseRapid onsetSensitive dye BCECFInhibitor omeprazoleAcid loadOmeprazoleDye BCECFSecretion
2004
Extracellular polyamines regulate fluid secretion in rat colonic crypts via the extracellular calcium-sensing receptor
Cheng SX, Geibel JP, Hebert SC. Extracellular polyamines regulate fluid secretion in rat colonic crypts via the extracellular calcium-sensing receptor. Gastroenterology 2004, 126: 148-158. PMID: 14699496, DOI: 10.1053/j.gastro.2003.10.064.Peer-Reviewed Original ResearchConceptsCalcium-sensing receptorColonic epithelial cellsFluid secretionColonic cryptsCalcium-sensing receptor activationExtracellular calcium-sensing receptorEpithelial cellsIntracellular D-myo-inositolNormal postnatal developmentRat colonic cryptsExtracellular polyaminesDistal colonDistal colonic cryptsSecond messenger generationDistal colonic epithelial cellsGastrointestinal epitheliumSecond messenger accumulationPostnatal developmentReceptor activationExtracellular spermineIntact cryptsReceptorsColonocyte functionSecretionCrypts
2003
Demonstration of a functional apical sodium hydrogen exchanger in isolated rat gastric glands
Kirchhoff P, Wagner CA, Gaetzschmann F, Radebold K, Geibel JP. Demonstration of a functional apical sodium hydrogen exchanger in isolated rat gastric glands. AJP Gastrointestinal And Liver Physiology 2003, 285: g1242-g1248. PMID: 12907430, DOI: 10.1152/ajpgi.00165.2003.Peer-Reviewed Original ResearchAmilorideAnimalsBlotting, WesternCell MembraneEnzyme InhibitorsGastric MucosaGuanidinesH(+)-K(+)-Exchanging ATPaseHistamineHydrogen-Ion ConcentrationImmunohistochemistryIn Vitro TechniquesIntracellular MembranesIsoenzymesMethacrylatesOmeprazoleParietal Cells, GastricPerfusionRatsRats, Sprague-DawleySodium-Hydrogen Exchanger 3Sodium-Hydrogen Exchangers
2002
Expression of calcium-sensing receptor in rat colonic epithelium: evidence for modulation of fluid secretion
Cheng SX, Okuda M, Hall AE, Geibel JP, Hebert SC. Expression of calcium-sensing receptor in rat colonic epithelium: evidence for modulation of fluid secretion. AJP Gastrointestinal And Liver Physiology 2002, 283: g240-g250. PMID: 12065312, DOI: 10.1152/ajpgi.00500.2001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportBody FluidsCalciumCell LineCell MembraneColonHumansIn Vitro TechniquesIntestinal MucosaIntracellular MembranesMalePhosphatidylinositol Diacylglycerol-LyaseRatsRats, Sprague-DawleyReceptors, Calcium-SensingReceptors, Cell SurfaceTranscription, GeneticType C PhospholipasesConceptsCalcium-sensing receptorCrypt cellsFluid secretionNet fluid secretionIntestinal fluid transportRat colonic epitheliumCaSR agonistsIntracellular calciumExtracellular calciumColonic epitheliumDiarrheal diseaseCaSR transcriptsBath perfusateStimulatory effectBody cellsSecretionReceptorsCryptsElevated cAMPBasolateral surfaceCellsDivalent mineralsCalciumCaSRAgonists
2001
Na+-dependent fluid absorption in intact perfused rat colonic crypts
Geibel J, Rajendran V, Binder H. Na+-dependent fluid absorption in intact perfused rat colonic crypts. Gastroenterology 2001, 120: 144-150. PMID: 11208723, DOI: 10.1053/gast.2001.20890.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBicarbonatesChloridesColonGanglionic StimulantsGlutamatesIn Vitro TechniquesIntestinal AbsorptionInulinIsotonic SolutionsMalePerfusionQuaternary Ammonium CompoundsRatsRats, Sprague-DawleyRinger's SolutionSodiumSodium-Hydrogen ExchangersTritiumConceptsNet fluid absorptionRat colonic cryptsCl- channel blockerFluid absorptionNeurohumoral stimulationChannel blockersColonic cryptsLumen ClAbsorptive processesInhibitor of NaCl(-) secretionAbstractTextCrypt cellsExchange inhibitorMicroperfusion techniqueFluid secretionL DIDSAmiloride analoguesMammalian colonSecretionAnion exchange inhibitorCryptsSignificant increaseAIMSSecretory process
1999
Role of Cl channels in Cl-dependent Na/H exchange
Rajendran V, Geibel J, Binder H. Role of Cl channels in Cl-dependent Na/H exchange. American Journal Of Physiology 1999, 276: g73-g78. PMID: 9886981, DOI: 10.1152/ajpgi.1999.276.1.g73.Peer-Reviewed Original ResearchConceptsNa/H exchangeCl- channel blockerChannel blockersMicroM DIDSNa/H exchange activityCystic fibrosis transmembrane conductance regulatorIntracellular pH recoveryRat distal colonCl- channelsH exchange activityDistal colonCrypt lumenCrypt cellsAcid loadExchange inhibitorApical membranePH recoveryCl(-) dependenceAnion exchange inhibitorCl- channel activityChannel activityBlockersH exchangePolyclonal antibodiesCl- conductance
1995
An apical permeability barrier to NH3/NH4+ in isolated, perfused colonic crypts.
Singh S, Binder H, Geibel J, Boron W. An apical permeability barrier to NH3/NH4+ in isolated, perfused colonic crypts. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 11573-11577. PMID: 8524806, PMCID: PMC40444, DOI: 10.1073/pnas.92.25.11573.Peer-Reviewed Original ResearchFluid absorption in isolated perfused colonic crypts.
Singh S, Binder H, Boron W, Geibel J. Fluid absorption in isolated perfused colonic crypts. Journal Of Clinical Investigation 1995, 96: 2373-2379. PMID: 7593625, PMCID: PMC185889, DOI: 10.1172/jci118294.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell PolarityColonCyclic AMPIn Vitro TechniquesIntestinal AbsorptionIntestinal MucosaMalePerfusionRatsRats, Sprague-DawleyConceptsCrypt cellsNM vasoactive intestinal peptideFluid absorptionLarge intestinal functionColonic cryptsVasoactive intestinal peptideLamina propria cellsNet fluid secretionRat distal colonNet fluid absorptionDibutyryl cyclic AMPIntestinal peptideDistal colonIntestinal functionLamina propriaPathophysiological paradigmCrypt preparationMM acetylcholineBasal stateNeurohumoral agonistsFluid secretionCellular elementsEpithelial cellsIntestinal cryptsCryptsGastrin-stimulated changes in Ca2+ concentration in parietal cells depends on adenosine 3′,5′-cyclic monophosphate levels
Geibel J, Abraham R, Modlin I, Sachs G. Gastrin-stimulated changes in Ca2+ concentration in parietal cells depends on adenosine 3′,5′-cyclic monophosphate levels. Gastroenterology 1995, 109: 1060-1067. PMID: 7557070, DOI: 10.1016/0016-5085(95)90563-4.Peer-Reviewed Original ResearchConceptsCyclic monophosphate (cAMP) levelsParietal cellsECL cellsIntracellular calcium concentration ([Ca2+]i) responseMonophosphate levelsECL-cell responseGastric glandsFunction of adenosineGastrin/cholecystokinin B receptorCholecystokinin B receptorCyclic monophosphate (cAMP) pathwayParietal cell functionAddition of gastrinH2 receptor activityEffect of gastrinIsolated gastric glandsSecretory receptorCell responsesGastrinReceptor activityAbstractTextB receptorCell functionIntracellular adenosineAIMSChloride-dependent Na-H Exchange A NOVEL MECHANISM OF SODIUM TRANSPORT IN COLONIC CRYPTS*
Rajendran V, Geibel J, Binder H. Chloride-dependent Na-H Exchange A NOVEL MECHANISM OF SODIUM TRANSPORT IN COLONIC CRYPTS*. Journal Of Biological Chemistry 1995, 270: 11051-11054. PMID: 7744735, DOI: 10.1074/jbc.270.19.11051.Peer-Reviewed Original ResearchConceptsNa-H exchangeColonic crypt cellsCrypt cellsAcid loadRat distal colonApical membrane vesiclesNovel transport processRate of intracellularDistal colonMM amilorideSodium transportApical membraneGradient stimulationColonic cryptsAmiloride inhibitionSodium movementNovel mechanismOutward gradientCompelling evidenceCellsSodiumUptakePrior studiesColonRecovery
1994
Unique Permeability Barrier of the Apical Surface of Parietal and Chief Cells in Isolated Perfused Gastric Glands
Boron W, Waisbren S, Modlin I, Geibel J. Unique Permeability Barrier of the Apical Surface of Parietal and Chief Cells in Isolated Perfused Gastric Glands. Journal Of Experimental Biology 1994, 196: 347-360. PMID: 7823033, DOI: 10.1242/jeb.196.1.347.Peer-Reviewed Original ResearchLuminal perfusion of isolated gastric glands
Waisbren S, Geibel J, Boron W, Modlin I. Luminal perfusion of isolated gastric glands. American Journal Of Physiology 1994, 266: c1013-c1027. PMID: 8178950, DOI: 10.1152/ajpcell.1994.266.4.c1013.Peer-Reviewed Original ResearchUnusual permeability properties of gastric gland cells
Waisbren S, Geibel J, Modlin I, Boron W. Unusual permeability properties of gastric gland cells. Nature 1994, 368: 332-335. PMID: 8127367, DOI: 10.1038/368332a0.Peer-Reviewed Original ResearchLiving Renal Epithelial Cells Imaged by Atomic Force Microscopy
Oberleithner H, Schwab A, Wang W, Giebisch G, Hume F, Geibel J. Living Renal Epithelial Cells Imaged by Atomic Force Microscopy. Nephron 1994, 66: 8-13. PMID: 8107959, DOI: 10.1159/000187759.Peer-Reviewed Original Research
1990
Angiotensin II stimulates both Na(+)-H+ exchange and Na+/HCO3- cotransport in the rabbit proximal tubule.
Geibel J, Giebisch G, Boron W. Angiotensin II stimulates both Na(+)-H+ exchange and Na+/HCO3- cotransport in the rabbit proximal tubule. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 7917-7920. PMID: 2172967, PMCID: PMC54862, DOI: 10.1073/pnas.87.20.7917.Peer-Reviewed Original ResearchConceptsAngiotensin IIHCO3- reabsorptionProximal tubulesEffect of AIIS1 proximal tubulesNM angiotensin IIPH-sensitive fluorescent dye 2Rat proximal tubuleRabbit proximal tubuleSuperficial nephronsPotent stimulusBasolateral administrationRabbit kidneyFluorescent dye 2Basolateral solutionBasolateral stepTransporter activityTubulesIonic mechanismsCotransportKidneyAdministrationNephron