2011
Functional role of NHE4 as a pH regulator in rat and human colonic crypts
Arena EA, Longo WE, Roberts KE, Geibel P, Nateqi J, Brandstetter M, Geibel JP. Functional role of NHE4 as a pH regulator in rat and human colonic crypts. American Journal Of Physiology - Cell Physiology 2011, 302: c412-c418. PMID: 22049213, DOI: 10.1152/ajpcell.00163.2011.Peer-Reviewed Original ResearchConceptsHuman colonic cryptsNHE4 activityColonic cryptsElective colon resectionEffects of aldosteroneSprague-Dawley ratsColon resectionRat modelGastrointestinal tractAldosteroneColonic samplesΜM forskolinFluid homeostasisRatsHuman colonHuman specimensCAMP levelsIntracellular pathwaysCryptsNHE4ColonSignificant changesLittle descriptionFunctional roleRecent studies
2004
Nongenomic stimulation of vacuolar H+-ATPases in intercalated renal tubule cells by aldosterone
Winter C, Schulz N, Giebisch G, Geibel JP, Wagner CA. Nongenomic stimulation of vacuolar H+-ATPases in intercalated renal tubule cells by aldosterone. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 2636-2641. PMID: 14983061, PMCID: PMC357002, DOI: 10.1073/pnas.0307321101.Peer-Reviewed Original ResearchConceptsAcid-base homeostasisMineralocorticoid receptorStimulatory effectAldosterone-induced stimulationSystemic acid-base homeostasisDistal tubular acidosisRenal tubule cellsAcid-base statusNongenomic modulationNongenomic stimulationRenal collecting ductsAngiotensin IITransepithelial pH gradientTubular acidosisUrine acidificationAldosteroneATPase activityRenal acidificationHydrogen secretionIntracellular Ca2Complete reabsorptionATPase stainingTubule cellsCollecting ductsNongenomic pathways
1999
Rapid Effects of Aldosterone on Sodium-Hydrogen Exchange in Isolated Colonic Crypts
Winter D, Schneider M, O'Sullivan G, Harvey B, Geibel J. Rapid Effects of Aldosterone on Sodium-Hydrogen Exchange in Isolated Colonic Crypts. The Journal Of Membrane Biology 1999, 170: 17-26. PMID: 10398757, DOI: 10.1007/s002329900534.Peer-Reviewed Original ResearchMeSH KeywordsAldosteroneAmilorideAnimalsArachidonic AcidBicarbonatesColonDiureticsExtracellular SpaceFluoresceinsFluorescent DyesHydrogen-Ion ConcentrationIon ExchangeIon TransportLeukotriene AntagonistsMaleMineralocorticoid Receptor AntagonistsPhospholipasesProstaglandin AntagonistsProtein BiosynthesisProtein Kinase InhibitorsProtein KinasesProtein Synthesis InhibitorsRatsRats, Sprague-DawleySodiumSodium-Hydrogen ExchangersSpironolactoneTranscription, GeneticConceptsProtein synthesisRapid cellular responsesSignal transduction pathwaysProtein kinase CSodium-hydrogen exchangeTransduction pathwaysColonic cryptsGenomic eventsCellular responsesKinase CG proteinsIsolated colonic cryptsConfocal laser imagingIntracellular receptorsTransepithelial electrolyte transportEpithelial ion transportNonepithelial tissuesHomeostatic regulationAldosterone applicationTraditional receptorsCentral roleIntracellular alkalizationIon transportReal-time imagingSteroid action
1998
Atomic Force Microscopy on Living Cells: Aldosterone–Induced Localized Cell Swelling
Schneider SW, Pagel P, Storck J, Yano Y, Sumpio BE, Geibel JP, Oberleithner H. Atomic Force Microscopy on Living Cells: Aldosterone–Induced Localized Cell Swelling. Kidney & Blood Pressure Research 1998, 21: 256-258. PMID: 9762848, DOI: 10.1159/000025869.Peer-Reviewed Original Research
1997
RAPID ALDOSTERONE‐INDUCED CELL VOLUME INCREASE OF ENDOTHELIAL CELLS MEASURED BY THE ATOMIC FORCE MICROSCOPE
SCHNEIDER S, YANO Y, SUMPIO B, JENA B, GEIBEL J, GEKLE M, OBERLEITHNER H. RAPID ALDOSTERONE‐INDUCED CELL VOLUME INCREASE OF ENDOTHELIAL CELLS MEASURED BY THE ATOMIC FORCE MICROSCOPE. Cell Biology International 1997, 21: 759-768. PMID: 9768474, DOI: 10.1006/cbir.1997.0220.Peer-Reviewed Original ResearchMeSH KeywordsAldosteroneAnimalsCattleCell SizeCells, CulturedEndothelium, VascularHumansMicroscopy, Atomic ForceConceptsEndothelial cellsAddition of aldosteroneArterial blood pressureNon-genomic effectsCultured endothelial cellsBlood pressureMineralocorticoid hormonesHormone exposureAldosteroneCell volumeHuman leukocytesIntracellular electrolytesTransient swellingTriphosphate productionIntracellular pHLeukocytesVolume increaseBovine aortaContinuous presenceCellsPlasma membrane
1989
Mechanism of aldosterone-induced increase of K+ conductance in early distal renal tubule cells of the frog
Wang W, Henderson R, Geibel J, White S, Giebisch G. Mechanism of aldosterone-induced increase of K+ conductance in early distal renal tubule cells of the frog. The Journal Of Membrane Biology 1989, 111: 277-289. PMID: 2557452, DOI: 10.1007/bf01871012.Peer-Reviewed Original ResearchConceptsEarly distal tubule cellsTubule cellsDistal renal tubule cellsFrog kidneyWhole-cell voltage-clamp techniqueRemoval of aldosteronePresence of aldosteroneAldosterone-induced increaseType II channelsDistal tubule cellsOuabain-treated cellsPatch-clamp techniqueRenal tubule cellsVoltage-clamp techniquePresence of furosemideAldosterone treatmentAldosteroneIncubation of cellsKidneyOpen probabilityDistal cellsHrCellsIntracellularComplete absence