2010
Zinc Salts Provide a Novel, Prolonged and Rapid Inhibition of Gastric Acid Secretion
Kirchhoff P, Socrates T, Sidani S, Duffy A, Breidthardt T, Grob C, Viehl CT, Beglinger C, Oertli D, Geibel JP. Zinc Salts Provide a Novel, Prolonged and Rapid Inhibition of Gastric Acid Secretion. The American Journal Of Gastroenterology 2010, 106: 62. PMID: 20736941, DOI: 10.1038/ajg.2010.327.Peer-Reviewed Original ResearchConceptsProton pump inhibitorsGastric acid secretionAcid secretionRat gastric glandsRandomized cross-over studyIntact rat stomachAcid suppression therapyGastric glandsCross-over studyOnset of actionLong-term efficacyReturn of symptomsHealthy human volunteersSecretagogue-induced acid secretionStomach acid secretionProlonged therapySuppression therapyIntragastric pHPump inhibitorsSingle doseWhole stomachLifetime prevalenceRat modelRat stomachFaster onset
2007
Intragastric Injection of Botulinum Toxin A for the Treatment of Obesity
Mittermair R, Keller C, Geibel J. Intragastric Injection of Botulinum Toxin A for the Treatment of Obesity. Obesity Surgery 2007, 17: 732-736. PMID: 17879570, DOI: 10.1007/s11695-007-9135-x.Peer-Reviewed Original ResearchConceptsTreatment of obesityDistal gastric bodyEarly satietyGastric bodyIntragastric injectionGroup 2Group 1Body weightClass I obesityBotulinum toxin AFeeling of satietyBackgroundBotulinum toxinU BTXObese patientsFemale patientsGastric motilityResultsBoth groupsMethodsAfter approvalToxin ASmooth muscleUniversity Ethics CommitteeEndoscopic proceduresPatientsObesitySatietyStimulatory Pathways of the Calcium-Sensing Receptor on Acid Secretion in Freshly Isolated Human Gastric Glands
Remy C, Kirchhoff P, Hafner P, Busque S, Mueller M, Geibel J, Wagner C. Stimulatory Pathways of the Calcium-Sensing Receptor on Acid Secretion in Freshly Isolated Human Gastric Glands. Cellular Physiology And Biochemistry 2007, 19: 33-42. PMID: 17310098, DOI: 10.1159/000099190.Peer-Reviewed Original ResearchConceptsHeterotrimeric G proteinsHuman gastric glandsMAP kinaseGastric parietal cellsATPase activityG proteinsGastric acid secretionERK 1/2 MAP kinaseIndependent PKC isoformsStimulation of CaSRERK1/2 MAP kinasesAcid secretionProtein kinase CPKC-delta isoformParietal cellsStimulatory effectChelator of intracellularActivation of PKCGastric glandsHuman gastric parietal cellsPLC inhibitor U73122Sensitive heterotrimeric G proteinsPD 098059General inhibitorPKC isoforms
2006
The KCNE2 Potassium Channel Ancillary Subunit Is Essential for Gastric Acid Secretion*
Roepke TK, Anantharam A, Kirchhoff P, Busque SM, Young JB, Geibel JP, Lerner DJ, Abbott GW. The KCNE2 Potassium Channel Ancillary Subunit Is Essential for Gastric Acid Secretion*. Journal Of Biological Chemistry 2006, 281: 23740-23747. PMID: 16754665, DOI: 10.1074/jbc.m604155200.Peer-Reviewed Original ResearchConceptsAncillary subunitsKCNE gene familyGastric acid secretionPotassium channel alpha subunitFirst genetic evidenceSecretory cellsProton secretionChannel alpha subunitGene familyKCNE familyParietal cell morphologyAcid secretionGenetic evidenceAlpha subunitHuman cardiac arrhythmiasParietal cellsChannel complexSubunitsCell morphologyKCNE2Normal gastrointestinal functionGenesKCNQ1Gene-dose effectCells
2005
The calcium-sensing receptor acts as a modulator of gastric acid secretion in freshly isolated human gastric glands
Dufner MM, Kirchhoff P, Remy C, Hafner P, Müller M, Cheng SX, Tang LQ, Hebert SC, Geibel JP, Wagner CA. The calcium-sensing receptor acts as a modulator of gastric acid secretion in freshly isolated human gastric glands. AJP Gastrointestinal And Liver Physiology 2005, 289: g1084-g1090. PMID: 16109841, DOI: 10.1152/ajpgi.00571.2004.Peer-Reviewed Original ResearchConceptsHuman gastric glandsHistamine-induced activationGastric acid secretionGastric glandsExtracellular divalent cationsParietal cellsAcid secretionIsolated rat gastric glandsRelease of acetylcholineCalcium-sensing receptorEnterochromaffin-like cellsAbsence of histamineExtracellular divalent cation concentrationFunctional CaSRATPase activityVagus nerveNeuronal pathwaysRat gastric glandsFura-2Human patientsCaSRFluo-4Sustained increaseCalcium measurementsHistamine
2000
Cell pH and H+ Secretion by S3 Segment of Mammalian Kidney: Role of H+-ATPase and Cl−
Malnic G, Geibel J. Cell pH and H+ Secretion by S3 Segment of Mammalian Kidney: Role of H+-ATPase and Cl−. The Journal Of Membrane Biology 2000, 178: 115-125. PMID: 11083900, DOI: 10.1007/s002320010019.Peer-Reviewed Original ResearchConceptsProximal tubule cellsChannel blocker NPPBS3 segmentTubule cellsProximal S3 segmentsFree mediumCell pHConfocal microscopyNIH Image programCell pH regulationMicroperfusion techniqueSuperfusionMouse kidneyRabbit kidneyKidneySubcellular vesiclesApical areaCell acidificationMammalian kidneyFluorescence densityNPPBRingerCellsImage programNaCl Ringer
1997
AN ATOMIC FORCE MICROSCOPIC EXAMINATION OF THE APICAL MEMBRANE OF THE MAMMALIAN GASTRIC GLAND
GEIBEL J. AN ATOMIC FORCE MICROSCOPIC EXAMINATION OF THE APICAL MEMBRANE OF THE MAMMALIAN GASTRIC GLAND. Cell Biology International 1997, 21: 727-736. PMID: 9768471, DOI: 10.1006/cbir.1997.0217.Peer-Reviewed Original Research
1995
An apical permeability barrier to NH3/NH4+ in isolated, perfused colonic crypts.
Singh S, Binder H, Geibel J, Boron W. An apical permeability barrier to NH3/NH4+ in isolated, perfused colonic crypts. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 11573-11577. PMID: 8524806, PMCID: PMC40444, DOI: 10.1073/pnas.92.25.11573.Peer-Reviewed Original Research
1993
Mechanism of apical K+ channel modulation in principal renal tubule cells. Effect of inhibition of basolateral Na(+)-K(+)-ATPase.
Wang W, Geibel J, Giebisch G. Mechanism of apical K+ channel modulation in principal renal tubule cells. Effect of inhibition of basolateral Na(+)-K(+)-ATPase. The Journal Of General Physiology 1993, 101: 673-694. PMID: 8393065, PMCID: PMC2216783, DOI: 10.1085/jgp.101.5.673.Peer-Reviewed Original ResearchConceptsEffect of inhibitionPump inhibitionInhibitory effectChannel activityProtein kinase CPump activityAddition of strophanthidinPatch-clamp techniqueRenal tubule cellsBath solutionCell-attached patchesExtracellular Ca2Removal of Ca2Intracellular Ca2MicroM ionomycinTubule cellsControl valuesLow-conductance K channelsPrincipal cellsChannel modulationK channelsInhibitionNM staurosporineDependent protein kinase CPotent inhibitor
1990
Angiotensin II stimulates both Na(+)-H+ exchange and Na+/HCO3- cotransport in the rabbit proximal tubule.
Geibel J, Giebisch G, Boron W. Angiotensin II stimulates both Na(+)-H+ exchange and Na+/HCO3- cotransport in the rabbit proximal tubule. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 7917-7920. PMID: 2172967, PMCID: PMC54862, DOI: 10.1073/pnas.87.20.7917.Peer-Reviewed Original ResearchConceptsAngiotensin IIHCO3- reabsorptionProximal tubulesEffect of AIIS1 proximal tubulesNM angiotensin IIPH-sensitive fluorescent dye 2Rat proximal tubuleRabbit proximal tubuleSuperficial nephronsPotent stimulusBasolateral administrationRabbit kidneyFluorescent dye 2Basolateral solutionBasolateral stepTransporter activityTubulesIonic mechanismsCotransportKidneyAdministrationNephronA potassium channel in the apical membrane of rabbit thick ascending limb of Henle's loop
Wang W, White S, Geibel J, Giebisch G. A potassium channel in the apical membrane of rabbit thick ascending limb of Henle's loop. American Journal Of Physiology 1990, 258: f244-f253. PMID: 2309888, DOI: 10.1152/ajprenal.1990.258.2.f244.Peer-Reviewed Original ResearchConceptsCell-attached patchesHenle's loopPipette solutionPotassium channelsOutward slope conductanceRabbit kidneyBath solutionSlope conductanceThick ascending limbChannel activityPatch-clamp techniqueInward slope conductanceApical membraneVoltage-dependent mannerAscending limbSingle potassium channelsKidneyPossible intracellular regulatorsIntracellular regulatorsOpen probabilityLimb
1989
Effects of acetate on luminal acidification processes in the S3 segment of the rabbit proximal tubule
Geibel J, Giebisch G, Boron W. Effects of acetate on luminal acidification processes in the S3 segment of the rabbit proximal tubule. American Journal Of Physiology 1989, 257: f586-f594. PMID: 2801961, DOI: 10.1152/ajprenal.1989.257.4.f586.Peer-Reviewed Original Research
1986
Effects of ouabain and temperature on cell membrane potentials in isolated perfused straight proximal tubules of the mouse kidney
Völkl H, Geibel J, Greger R, Lang F. Effects of ouabain and temperature on cell membrane potentials in isolated perfused straight proximal tubules of the mouse kidney. Pflügers Archiv - European Journal Of Physiology 1986, 407: 252-257. PMID: 3763371, DOI: 10.1007/bf00585299.Peer-Reviewed Original Research