2020
Hydrogel‐based 3D bioprints repair rat small intestine injuries and integrate into native intestinal tissue
Maina RM, Barahona MJ, Geibel P, Lysyy T, Finotti M, Isaji T, Wengerter B, Mentone S, Dardik A, Geibel JP. Hydrogel‐based 3D bioprints repair rat small intestine injuries and integrate into native intestinal tissue. Journal Of Tissue Engineering And Regenerative Medicine 2020, 15: 129-138. PMID: 33197151, DOI: 10.1002/term.3157.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBioprintingHydrogelsIntestinal MucosaIntestine, SmallMalePrinting, Three-DimensionalRatsRats, WistarConceptsIntestinal segmentsPost-operative day 7Male Sprague-Dawley ratsMale Wistar ratsSmall intestine injurySprague-Dawley ratsNative intestineBlinded histopathological analysisIntestinal failureStool outputNative intestinal tissueIntestine injuryIntraluminal perfusionRat vascular cellsHistopathological analysisIleocecal junctionWistar ratsIntestinal integrityIntestinal tissueDay 7Small intestineGenetic malformationsNormal activityWeight gainEnterotomy
2019
Design and implementation of novel nutraceuticals and derivatives for treating intestinal disorders
Barahona MJ, Baratta V, Ollodart J, Mulligan D, Geibel JP. Design and implementation of novel nutraceuticals and derivatives for treating intestinal disorders. Future Medicinal Chemistry 2019, 11: 847-855. PMID: 30994367, PMCID: PMC8008207, DOI: 10.4155/fmc-2018-0313.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDietary SupplementsGastrointestinal DiseasesHumansIntestinal MucosaModels, MolecularNanoparticlesReceptors, Calcium-SensingConceptsSignificant worldwide disease burdenSpectrum of illnessWorldwide disease burdenIntestinal fluid secretionSystemic side effectsCalcium-sensing receptorNovel therapeutic targetG protein-coupled receptorsSurgical therapyPharmaceutical optionsMucosal integrityDisease burdenIntestinal disordersNutraceutical therapyGastrointestinal diseasesGastrointestinal illnessSide effectsTherapeutic targetFluid secretionIntestinal cellsTherapyAdverse effectsNovel nutraceuticalsCaSRIllness
2017
Acute Effects of Vitamin C Exposure On Colonic Crypts: Direct Modulation of pH Regulation
Aldajani MM, Vanicek CN, Alhazzaa N, Lysyy T, Agarwal R, Geibel JP. Acute Effects of Vitamin C Exposure On Colonic Crypts: Direct Modulation of pH Regulation. Cellular Physiology And Biochemistry 2017, 44: 377-387. PMID: 29132138, DOI: 10.1159/000484914.Peer-Reviewed Original ResearchConceptsVitamin CColonic cryptsAcute effectsDependent intracellular pH recoveryTumor formationIntracellular pH recoveryNew therapeutic agentsMM vitamin CColorectal cancerDistal colonLeading causeTranscellular passive diffusionBasolateral membraneAcute additionNormal colonic cryptsC exposureNutraceutical agentTherapeutic agentsPH recoveryIntracellular pH changesCancer cellsVitamin C.CryptsLow intracellularTight junctionsStomach and Bone
Kitay AM, Geibel JP. Stomach and Bone. Advances In Experimental Medicine And Biology 2017, 1033: 97-131. PMID: 29101653, DOI: 10.1007/978-3-319-66653-2_6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone and BonesCalciumGastric MucosaHomeostasisHumansIntestinal MucosaKidneyParathyroid HormoneReceptors, Calcium-SensingConceptsBone metabolismGastric acid secretionCalcium-sensing receptorVitamin DParathyroid glandsAcid secretionGastrointestinal organsTherapeutic implicationsEndocrine modulatorsCalcium homeostasisDiseaseStomachOrgansMetabolismImportant playersIntermediary organDiverse tissuesDysfunctionCaSRPTHCalcitoninKidneySecretionIntestineReceptors
2014
Calcium-sensing receptor 20 years later
Alfadda TI, Saleh AM, Houillier P, Geibel JP. Calcium-sensing receptor 20 years later. American Journal Of Physiology - Cell Physiology 2014, 307: c221-c231. PMID: 24871857, PMCID: PMC4121584, DOI: 10.1152/ajpcell.00139.2014.Peer-Reviewed Original ResearchConceptsCellular ion transport mechanismsG protein-coupled receptor familyProtein-coupled receptor familyEnvironmental cuesExtracellular environmentOrgan physiologyReceptor familySense changesDifferent tissuesCellular levelCalcium-sensing receptorIon transport mechanismsPhysiologyReceptor physiologyAmino acid concentrationsDisease statesReceptorsSystemic physiologyDiversity of locationsReceptor mutationsDifferent organ systemsImportant roleMembersFamilyHomology
2013
Activation of AMPK Inhibits Cholera Toxin Stimulated Chloride Secretion in Human and Murine Intestine
Rogers AC, Huetter L, Hoekstra N, Collins D, Collaco A, Baird AW, Winter DC, Ameen N, Geibel JP, Kopic S. Activation of AMPK Inhibits Cholera Toxin Stimulated Chloride Secretion in Human and Murine Intestine. PLOS ONE 2013, 8: e69050. PMID: 23935921, PMCID: PMC3728293, DOI: 10.1371/journal.pone.0069050.Peer-Reviewed Original ResearchConceptsIntestinal chloride secretionCholera toxinChloride secretionPharmacological activationFluid accumulationColonic cryptsSupplemental treatment strategyAcute diarrheal illnessChloride effluxCystic fibrosis transmembrane conductance regulatorExcised intestinal segmentsRat colonic cryptsMurine colonic mucosaMucosal sheetsDiarrheal illnessIntestinal loopsEnterotoxigenic diarrheaColonic mucosaTreatment strategiesIntestinal segmentsUssing chambersAMPK activator AICARMurine intestineTissue levelsMetforminGastric Acid, Calcium Absorption, and Their Impact on Bone Health
Kopic S, Geibel JP. Gastric Acid, Calcium Absorption, and Their Impact on Bone Health. Physiological Reviews 2013, 93: 189-268. PMID: 23303909, DOI: 10.1152/physrev.00015.2012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntacidsBone and BonesBone Diseases, MetabolicCalcitoninCalciumGastric AcidGastric MucosaHistamine H2 AntagonistsHomeostasisHumansHydrogen-Ion ConcentrationIntestinal AbsorptionIntestinal MucosaIntestinesParathyroid HormoneProton Pump InhibitorsReceptors, Calcium-SensingSignal TransductionVitamin DConceptsBone healthCalcium absorptionIntestinal calcium absorptionGastric acid secretionSystemic calcium homeostasisAdequate intestinal absorptionGastric acidityCalcium balanceGastric acidAcid secretionIntestinal absorptionCalcium homeostasisNeuroendocrine regulationCalcium uptakePhysiological processesPhysiological basisPotential linkHealthSecretionIntestine
2011
Hypoxia Inhibits Colonic Ion Transport via Activation of AMP Kinase
Collins D, Kopic S, Bachlechner J, Ritter M, Winter DC, Geibel JP. Hypoxia Inhibits Colonic Ion Transport via Activation of AMP Kinase. Annals Of Surgery 2011, 254: 957-963. PMID: 21562404, DOI: 10.1097/sla.0b013e31821d477f.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorChloride secretionMucosal hypoxiaAcute hypoxiaColonic electrolyte transportColonic mucosal functionIschemic intestinal injuryElectrogenic chloride secretionColonic ion transportPotential therapeutic targetHuman colonic mucosaIntracellular chloride concentrationRat colonic cryptsEpithelial ion transportFibrosis transmembrane conductance regulatorIschemic colitisIntestinal injuryColonic obstructionInhibition of CFTRMucosal functionAnastomotic failureAcute effectsColonic mucosaAdaptive cellular responseTransmembrane conductance regulator
2010
Toxin Mediated Diarrhea in the 21st Century: The Pathophysiology of Intestinal Ion Transport in the Course of ETEC, V. cholerae and Rotavirus Infection
Kopic S, Geibel JP. Toxin Mediated Diarrhea in the 21st Century: The Pathophysiology of Intestinal Ion Transport in the Course of ETEC, V. cholerae and Rotavirus Infection. Toxins 2010, 2: 2132-2157. PMID: 22069677, PMCID: PMC3153279, DOI: 10.3390/toxins2082132.Peer-Reviewed Original ResearchConceptsEpisodes of diarrheaIntestinal ion transportSpecific therapeutic approachesMajor healthcare concernRotavirus infectionTherapeutic approachesV. choleraeCommon enterotoxinInfectious agentsPathophysiological processesInfectious diseasesDiarrheaHealthcare concernCellular mechanismsLethal effectsPathophysiologyYearsInfectionCholeraeRotavirusDiseaseETECCommentary on ‘Diarrhoeal disease through enterocyte secretion: a doctrine untroubled by proof’
Kopic S, Murek M, Geibel JP. Commentary on ‘Diarrhoeal disease through enterocyte secretion: a doctrine untroubled by proof’. Quarterly Journal Of Experimental Physiology And Cognate Medical Sciences 2010, 95: 484-485. PMID: 20233894, DOI: 10.1113/expphysiol.2009.051912.Peer-Reviewed Original ResearchEvidence for intestinal chloride secretion
Murek M, Kopic S, Geibel J. Evidence for intestinal chloride secretion. Quarterly Journal Of Experimental Physiology And Cognate Medical Sciences 2010, 95: 471-478. PMID: 20233891, DOI: 10.1113/expphysiol.2009.049445.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChloride ChannelsChloridesCLC-2 Chloride ChannelsCystic Fibrosis Transmembrane Conductance RegulatorDiarrheaEnterocytesHumansIntestinal AbsorptionIntestinal MucosaIntestinal SecretionsPotassium ChannelsSodium-Potassium-Chloride SymportersSodium-Potassium-Exchanging ATPaseTight JunctionsWater-Electrolyte Balance
2009
The Functions and Roles of the Extracellular Ca2+–Sensing Receptor along the Gastrointestinal Tract
Geibel JP, Hebert SC. The Functions and Roles of the Extracellular Ca2+–Sensing Receptor along the Gastrointestinal Tract. Annual Review Of Physiology 2009, 71: 205-217. PMID: 19575679, DOI: 10.1146/annurev.physiol.010908.163128.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumGastrointestinal TractHomeostasisHumansIntestinal MucosaReceptors, Calcium-SensingConceptsGastrointestinal tractNormal gut physiologyNutrient availabilityDigestion of foodRapid turnoverAmino acidsColonic fluid transportFunctional digestive tractGastric acid secretionCalcium-sensing receptorOrganic nutrientsWater homeostasisEntire gastrointestinal tractPotent targetGut physiologyEpithelial cellsIntestinal epitheliumSecretory diarrheaAcid secretionDigestive tractExtracellular Ca2Colon cancerReceptorsNormal saltFluid secretion
2006
Role of calcium and other trace elements in the gastrointestinal physiology
Kirchhoff P, Geibel J. Role of calcium and other trace elements in the gastrointestinal physiology. World Journal Of Gastroenterology 2006, 12: 3229-3236. PMID: 16718844, PMCID: PMC4087967, DOI: 10.3748/wjg.v12.i20.3229.Peer-Reviewed Original ResearchConceptsCell type changesGastrointestinal tractTerrestrial organismsNutrient availabilityIntestinal epithelial cellsCellular behaviorTerminal differentiationExact functionEssential ionsAmino acidsNeuronal functionGastric acid secretionEpithelial cellsEntire gastrointestinal tractAllosteric modifierCaSR expressionRole of calciumElevated extracellularSecretory diarrheaAcid secretionCalcium handlingReceptorsNeoplastic diseaseGastrointestinal physiologyPhysiological effects
2005
Role of short-chain fatty acids in colonic HCO3 secretion
Vidyasagar S, Barmeyer C, Geibel J, Binder HJ, Rajendran VM. Role of short-chain fatty acids in colonic HCO3 secretion. AJP Gastrointestinal And Liver Physiology 2005, 288: g1217-g1226. PMID: 15677553, DOI: 10.1152/ajpgi.00415.2004.Peer-Reviewed Original ResearchConceptsShort-chain fatty acidsSCFA/SCFA transportApical membrane vesiclesMM butyrateSurface epithelial cellsFatty acidsSCFA metabolismColonic mucosaHCO3- secretionCrypt cellsCarrier-mediated processLucite chamberSecretionColonic cryptsEpithelial cellsComparable degreeButyrate stimulationChannel activityButyrate uptakeStat techniqueCryptsAnion channel activityDIDSButyrate
2004
Extracellular polyamines regulate fluid secretion in rat colonic crypts via the extracellular calcium-sensing receptor
Cheng SX, Geibel JP, Hebert SC. Extracellular polyamines regulate fluid secretion in rat colonic crypts via the extracellular calcium-sensing receptor. Gastroenterology 2004, 126: 148-158. PMID: 14699496, DOI: 10.1053/j.gastro.2003.10.064.Peer-Reviewed Original ResearchConceptsCalcium-sensing receptorColonic epithelial cellsFluid secretionColonic cryptsCalcium-sensing receptor activationExtracellular calcium-sensing receptorEpithelial cellsIntracellular D-myo-inositolNormal postnatal developmentRat colonic cryptsExtracellular polyaminesDistal colonDistal colonic cryptsSecond messenger generationDistal colonic epithelial cellsGastrointestinal epitheliumSecond messenger accumulationPostnatal developmentReceptor activationExtracellular spermineIntact cryptsReceptorsColonocyte functionSecretionCrypts
2002
Expression of calcium-sensing receptor in rat colonic epithelium: evidence for modulation of fluid secretion
Cheng SX, Okuda M, Hall AE, Geibel JP, Hebert SC. Expression of calcium-sensing receptor in rat colonic epithelium: evidence for modulation of fluid secretion. AJP Gastrointestinal And Liver Physiology 2002, 283: g240-g250. PMID: 12065312, DOI: 10.1152/ajpgi.00500.2001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportBody FluidsCalciumCell LineCell MembraneColonHumansIn Vitro TechniquesIntestinal MucosaIntracellular MembranesMalePhosphatidylinositol Diacylglycerol-LyaseRatsRats, Sprague-DawleyReceptors, Calcium-SensingReceptors, Cell SurfaceTranscription, GeneticType C PhospholipasesConceptsCalcium-sensing receptorCrypt cellsFluid secretionNet fluid secretionIntestinal fluid transportRat colonic epitheliumCaSR agonistsIntracellular calciumExtracellular calciumColonic epitheliumDiarrheal diseaseCaSR transcriptsBath perfusateStimulatory effectBody cellsSecretionReceptorsCryptsElevated cAMPBasolateral surfaceCellsDivalent mineralsCalciumCaSRAgonists
2001
Characterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon
Rajendran V, Geibel J, Binder H. Characterization of apical membrane Cl-dependent Na/H exchange in crypt cells of rat distal colon. AJP Gastrointestinal And Liver Physiology 2001, 280: g400-g405. PMID: 11171622, DOI: 10.1152/ajpgi.2001.280.3.g400.Peer-Reviewed Original ResearchConceptsRat distal colonNa/H exchangeCl- channel blockerDistal colonChannel blockersCrypt cellsIntracellular pH recoveryExchange inhibitor amilorideColonic epithelial cellsApical membrane vesiclesCrypt glandsHigh doseHOE 694NA depletionAcid loadExchange inhibitorInhibitor amiloridePH recoveryEpithelial cellsUptake 1NHE isoformsAnion exchange inhibitorBlockersColonNa uptake
2000
Cl‐Dependent Na‐H Exchange: A Novel Colonic Crypt Transport Mechanism
BINDER H, RAJENDRAN V, GEIBEL J. Cl‐Dependent Na‐H Exchange: A Novel Colonic Crypt Transport Mechanism. Annals Of The New York Academy Of Sciences 2000, 915: 43-53. PMID: 11193600, DOI: 10.1111/j.1749-6632.2000.tb05222.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportChloride ChannelsColonIntestinal MucosaSodium-Hydrogen Exchangers
1999
Direct Effects of Dopamine on Colonic Mucosal pH: Implications for Tonometry
Winter D, O'Sullivan G, Harvey B, Geibel J. Direct Effects of Dopamine on Colonic Mucosal pH: Implications for Tonometry. Journal Of Surgical Research 1999, 83: 62-68. PMID: 10210644, DOI: 10.1006/jsre.1998.5561.Peer-Reviewed Original ResearchConceptsSodium-hydrogen exchangeSplanchnic perfusionIntestinal mucosaLow-dose dopamine infusionBeta-adrenergic receptor blockadeEffects of dopamineSprague-Dawley ratsIntracellular calcium concentrationHepes-buffered Ringer's solutionMucosal crypt cellsFree cytosolic calciumCalcium-sensitive probeSplanchnic vasodilatorTonometric dataDopamine infusionMucosal effectsReceptor blockadeShocked patientsProtein kinase C inhibitionDistal colonGastric mucosaMucosal pHPharmacological agentsDirect effectTonometric measurements
1998
Role of Apical H-K Exchange and Basolateral K Channel in the Regulation of Intracellular pH in Rat Distal Colon Crypt Cells
Ikuma M, Binder H, Geibel J. Role of Apical H-K Exchange and Basolateral K Channel in the Regulation of Intracellular pH in Rat Distal Colon Crypt Cells. The Journal Of Membrane Biology 1998, 166: 205-212. PMID: 9843594, DOI: 10.1007/s002329900462.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiportersBariumCell PolarityColonEpithelial CellsH(+)-K(+)-Exchanging ATPaseHomeostasisHydrogen-Ion ConcentrationIntestinal MucosaIntracellular FluidIon TransportMaleOuabainPotassiumPotassium ChannelsPotassium-Hydrogen AntiportersRatsRats, Sprague-DawleySodium-Potassium-Exchanging ATPase