2004
Nongenomic stimulation of vacuolar H+-ATPases in intercalated renal tubule cells by aldosterone
Winter C, Schulz N, Giebisch G, Geibel JP, Wagner CA. Nongenomic stimulation of vacuolar H+-ATPases in intercalated renal tubule cells by aldosterone. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 2636-2641. PMID: 14983061, PMCID: PMC357002, DOI: 10.1073/pnas.0307321101.Peer-Reviewed Original ResearchConceptsAcid-base homeostasisMineralocorticoid receptorStimulatory effectAldosterone-induced stimulationSystemic acid-base homeostasisDistal tubular acidosisRenal tubule cellsAcid-base statusNongenomic modulationNongenomic stimulationRenal collecting ductsAngiotensin IITransepithelial pH gradientTubular acidosisUrine acidificationAldosteroneATPase activityRenal acidificationHydrogen secretionIntracellular Ca2Complete reabsorptionATPase stainingTubule cellsCollecting ductsNongenomic pathways
2003
A rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse
Wagner CA, Lükewille U, Valles P, Breton S, Brown D, Giebisch GH, Geibel JP. A rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse. Pflügers Archiv - European Journal Of Physiology 2003, 446: 623-632. PMID: 12748863, DOI: 10.1007/s00424-003-1082-3.Peer-Reviewed Original ResearchConceptsProximal tubulesTubule fragmentsMouse kidneyKidney tubule fragmentsCollagenase digestion techniqueMouse proximal tubulesUnits/minTrypan blue exclusionKidney functionInner stripeOuter medullaIntercalated cellsIndependent intracellularBlue exclusionTrypan blueTubulesKidneyMicePH-sensitive dyeLight microscopyDuctAnimalsFurther characterizationATPase activityFunctional characteristics
1994
Living Renal Epithelial Cells Imaged by Atomic Force Microscopy
Oberleithner H, Schwab A, Wang W, Giebisch G, Hume F, Geibel J. Living Renal Epithelial Cells Imaged by Atomic Force Microscopy. Nephron 1994, 66: 8-13. PMID: 8107959, DOI: 10.1159/000187759.Peer-Reviewed Original Research
1993
Mechanism of apical K+ channel modulation in principal renal tubule cells. Effect of inhibition of basolateral Na(+)-K(+)-ATPase.
Wang W, Geibel J, Giebisch G. Mechanism of apical K+ channel modulation in principal renal tubule cells. Effect of inhibition of basolateral Na(+)-K(+)-ATPase. The Journal Of General Physiology 1993, 101: 673-694. PMID: 8393065, PMCID: PMC2216783, DOI: 10.1085/jgp.101.5.673.Peer-Reviewed Original ResearchConceptsEffect of inhibitionPump inhibitionInhibitory effectChannel activityProtein kinase CPump activityAddition of strophanthidinPatch-clamp techniqueRenal tubule cellsBath solutionCell-attached patchesExtracellular Ca2Removal of Ca2Intracellular Ca2MicroM ionomycinTubule cellsControl valuesLow-conductance K channelsPrincipal cellsChannel modulationK channelsInhibitionNM staurosporineDependent protein kinase CPotent inhibitor
1990
A potassium channel in the apical membrane of rabbit thick ascending limb of Henle's loop
Wang W, White S, Geibel J, Giebisch G. A potassium channel in the apical membrane of rabbit thick ascending limb of Henle's loop. American Journal Of Physiology 1990, 258: f244-f253. PMID: 2309888, DOI: 10.1152/ajprenal.1990.258.2.f244.Peer-Reviewed Original ResearchConceptsCell-attached patchesHenle's loopPipette solutionPotassium channelsOutward slope conductanceRabbit kidneyBath solutionSlope conductanceThick ascending limbChannel activityPatch-clamp techniqueInward slope conductanceApical membraneVoltage-dependent mannerAscending limbSingle potassium channelsKidneyPossible intracellular regulatorsIntracellular regulatorsOpen probabilityLimbK+ Channels of the Mammalian Collecting Duct
Geibel J, Zweifach A, White S, Wang W, Giebisch G. K+ Channels of the Mammalian Collecting Duct. Kidney & Blood Pressure Research 1990, 13: 59-69. PMID: 1689862, DOI: 10.1159/000173348.Peer-Reviewed Original ResearchConceptsApical membraneCell membraneMammalian collecting ductsSmall conductanceApical cell membraneOpen probability increasesBasolateral cell membraneIon channelsGating propertiesBasolateral membraneHormone actionMajor siteMembraneCell depolarizationOpen probabilityPossible roleTransport systemConductance
1989
Mechanism of aldosterone-induced increase of K+ conductance in early distal renal tubule cells of the frog
Wang W, Henderson R, Geibel J, White S, Giebisch G. Mechanism of aldosterone-induced increase of K+ conductance in early distal renal tubule cells of the frog. The Journal Of Membrane Biology 1989, 111: 277-289. PMID: 2557452, DOI: 10.1007/bf01871012.Peer-Reviewed Original ResearchConceptsEarly distal tubule cellsTubule cellsDistal renal tubule cellsFrog kidneyWhole-cell voltage-clamp techniqueRemoval of aldosteronePresence of aldosteroneAldosterone-induced increaseType II channelsDistal tubule cellsOuabain-treated cellsPatch-clamp techniqueRenal tubule cellsVoltage-clamp techniquePresence of furosemideAldosterone treatmentAldosteroneIncubation of cellsKidneyOpen probabilityDistal cellsHrCellsIntracellularComplete absence
1986
Effects of ouabain and temperature on cell membrane potentials in isolated perfused straight proximal tubules of the mouse kidney
Völkl H, Geibel J, Greger R, Lang F. Effects of ouabain and temperature on cell membrane potentials in isolated perfused straight proximal tubules of the mouse kidney. Pflügers Archiv - European Journal Of Physiology 1986, 407: 252-257. PMID: 3763371, DOI: 10.1007/bf00585299.Peer-Reviewed Original Research
1984
A microelectrode for continuous monitoring of glucose concentration in isolated perfused tubule segments
Rehwald W, Geibel J, Gstrein E, Oberleithner H. A microelectrode for continuous monitoring of glucose concentration in isolated perfused tubule segments. Pflügers Archiv - European Journal Of Physiology 1984, 400: 398-402. PMID: 6462888, DOI: 10.1007/bf00587539.Peer-Reviewed Original ResearchA microelectrode for continuous recording of volume fluxes in isolated perfused tubule segments
Geibel J, Völkl H, Lang F. A microelectrode for continuous recording of volume fluxes in isolated perfused tubule segments. Pflügers Archiv - European Journal Of Physiology 1984, 400: 388-392. PMID: 6462886, DOI: 10.1007/bf00587537.Peer-Reviewed Original ResearchA microelectrode for continuous monitoring of redox activity in isolated perfused tubule segments
Völkl H, Geibel J, Rehwald W, Lang F. A microelectrode for continuous monitoring of redox activity in isolated perfused tubule segments. Pflügers Archiv - European Journal Of Physiology 1984, 400: 393-397. PMID: 6462887, DOI: 10.1007/bf00587538.Peer-Reviewed Original Research