2012
Effect of the AMP-Kinase Modulators AICAR, Metformin and Compound C on Insulin Secretion of INS-1E Rat Insulinoma Cells under Standard Cell Culture Conditions
Langelueddecke C, Jakab M, Ketterl N, Lehner L, Hufnagl C, Schmidt S, Geibel JP, Fuerst J, Ritter M. Effect of the AMP-Kinase Modulators AICAR, Metformin and Compound C on Insulin Secretion of INS-1E Rat Insulinoma Cells under Standard Cell Culture Conditions. Cellular Physiology And Biochemistry 2012, 29: 75-86. PMID: 22415077, DOI: 10.1159/000337589.Peer-Reviewed Original ResearchConceptsAMP-dependent protein kinaseCompound CStandard cell culture conditionsCell culture conditionsINS-1E rat insulinoma cellsAMPK phosphorylationAMPK inhibitor compound CCulture conditionsCell membrane potentialAMPK-independent mechanismCell metabolic stateInhibitor compound CINS-1E cellsMetabolic stateMembrane potentialStimulus-secretion couplingBasal AMPK phosphorylationΒ-cell survivalCell cycle arrestProtein kinaseΒ-cell line INS-1E.Nutrient uptakeAMPK activatorCell cycle distributionInsulin secretion
2011
Hypoxia Inhibits Colonic Ion Transport via Activation of AMP Kinase
Collins D, Kopic S, Bachlechner J, Ritter M, Winter DC, Geibel JP. Hypoxia Inhibits Colonic Ion Transport via Activation of AMP Kinase. Annals Of Surgery 2011, 254: 957-963. PMID: 21562404, DOI: 10.1097/sla.0b013e31821d477f.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorChloride secretionMucosal hypoxiaAcute hypoxiaColonic electrolyte transportColonic mucosal functionIschemic intestinal injuryElectrogenic chloride secretionColonic ion transportPotential therapeutic targetHuman colonic mucosaIntracellular chloride concentrationRat colonic cryptsEpithelial ion transportFibrosis transmembrane conductance regulatorIschemic colitisIntestinal injuryColonic obstructionInhibition of CFTRMucosal functionAnastomotic failureAcute effectsColonic mucosaAdaptive cellular responseTransmembrane conductance regulator
2005
Fluorescent imaging of Cl- in Amphiuma red blood cells: How the nuclear exclusion of Cl- affects the plasma membrane potential
Hoffman JF, Geibel JP. Fluorescent imaging of Cl- in Amphiuma red blood cells: How the nuclear exclusion of Cl- affects the plasma membrane potential. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 921-926. PMID: 15637151, PMCID: PMC545567, DOI: 10.1073/pnas.0408597102.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCell NucleusChlorineErythrocytesMembrane PotentialsMicroscopy, FluorescenceNitratesUrodelaConceptsPlasma membrane potentialMembrane potentialExclusion of ClNuclear exclusionCytoplasmic compartmentCellular ClAmphiuma red blood cellsCytoplasmNet negative chargeValues of EmCellsIntracellular ClRed blood cellsBlood cellsCell ClDistribution of ClFluorescent imagingEquilibrium distributionDNANucleusFluorescence intensitySensitive dyeCompartmentsRed cellsNegative charge
1993
Mechanism of activation of K++ channels by minoxidil-sulfate in Madin-Darby canine kidney cells
Schwab A, Geibel J, Wang W, Oberleithner H, Giebisch G. Mechanism of activation of K++ channels by minoxidil-sulfate in Madin-Darby canine kidney cells. The Journal Of Membrane Biology 1993, 132: 125-136. PMID: 8496944, DOI: 10.1007/bf00239002.Peer-Reviewed Original Research
1989
Mechanism of aldosterone-induced increase of K+ conductance in early distal renal tubule cells of the frog
Wang W, Henderson R, Geibel J, White S, Giebisch G. Mechanism of aldosterone-induced increase of K+ conductance in early distal renal tubule cells of the frog. The Journal Of Membrane Biology 1989, 111: 277-289. PMID: 2557452, DOI: 10.1007/bf01871012.Peer-Reviewed Original ResearchConceptsEarly distal tubule cellsTubule cellsDistal renal tubule cellsFrog kidneyWhole-cell voltage-clamp techniqueRemoval of aldosteronePresence of aldosteroneAldosterone-induced increaseType II channelsDistal tubule cellsOuabain-treated cellsPatch-clamp techniqueRenal tubule cellsVoltage-clamp techniquePresence of furosemideAldosterone treatmentAldosteroneIncubation of cellsKidneyOpen probabilityDistal cellsHrCellsIntracellularComplete absenceEffects of acetate on luminal acidification processes in the S3 segment of the rabbit proximal tubule
Geibel J, Giebisch G, Boron W. Effects of acetate on luminal acidification processes in the S3 segment of the rabbit proximal tubule. American Journal Of Physiology 1989, 257: f586-f594. PMID: 2801961, DOI: 10.1152/ajprenal.1989.257.4.f586.Peer-Reviewed Original Research
1986
Effects of ouabain and temperature on cell membrane potentials in isolated perfused straight proximal tubules of the mouse kidney
Völkl H, Geibel J, Greger R, Lang F. Effects of ouabain and temperature on cell membrane potentials in isolated perfused straight proximal tubules of the mouse kidney. Pflügers Archiv - European Journal Of Physiology 1986, 407: 252-257. PMID: 3763371, DOI: 10.1007/bf00585299.Peer-Reviewed Original Research