2003
A rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse
Wagner CA, Lükewille U, Valles P, Breton S, Brown D, Giebisch GH, Geibel JP. A rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse. Pflügers Archiv - European Journal Of Physiology 2003, 446: 623-632. PMID: 12748863, DOI: 10.1007/s00424-003-1082-3.Peer-Reviewed Original ResearchConceptsProximal tubulesTubule fragmentsMouse kidneyKidney tubule fragmentsCollagenase digestion techniqueMouse proximal tubulesUnits/minTrypan blue exclusionKidney functionInner stripeOuter medullaIntercalated cellsIndependent intracellularBlue exclusionTrypan blueTubulesKidneyMicePH-sensitive dyeLight microscopyDuctAnimalsFurther characterizationATPase activityFunctional characteristics
1997
Gi regulation of secretory vesicle swelling examined by atomic force microscopy
Jena B, Schneider S, Geibel J, Webster P, Oberleithner H, Sritharan K. Gi regulation of secretory vesicle swelling examined by atomic force microscopy. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 13317-13322. PMID: 9371843, PMCID: PMC24306, DOI: 10.1073/pnas.94.24.13317.Peer-Reviewed Original ResearchConceptsSecretory vesicle membraneVesicle swellingSecretory vesiclesPlasma membraneVesicle fusionVesicle membraneSecretory vesicle swellingSecretory vesicle fusionConfocal microscopyZymogen granulesCell plasma membraneVesicle volumeHeterotrimeric guanineHeterotrimeric GTPZymogen granule sizeGTPase activityAlpha subunitMolecular mechanismsRegulatory rolePresence of GTPExocytotic processMastoparan analogsProteinGi proteinsMas7