2011
Hypoxia Inhibits Colonic Ion Transport via Activation of AMP Kinase
Collins D, Kopic S, Bachlechner J, Ritter M, Winter DC, Geibel JP. Hypoxia Inhibits Colonic Ion Transport via Activation of AMP Kinase. Annals Of Surgery 2011, 254: 957-963. PMID: 21562404, DOI: 10.1097/sla.0b013e31821d477f.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorChloride secretionMucosal hypoxiaAcute hypoxiaColonic electrolyte transportColonic mucosal functionIschemic intestinal injuryElectrogenic chloride secretionColonic ion transportPotential therapeutic targetHuman colonic mucosaIntracellular chloride concentrationRat colonic cryptsEpithelial ion transportFibrosis transmembrane conductance regulatorIschemic colitisIntestinal injuryColonic obstructionInhibition of CFTRMucosal functionAnastomotic failureAcute effectsColonic mucosaAdaptive cellular responseTransmembrane conductance regulator
2005
Fluorescent imaging of Cl- in Amphiuma red blood cells: How the nuclear exclusion of Cl- affects the plasma membrane potential
Hoffman JF, Geibel JP. Fluorescent imaging of Cl- in Amphiuma red blood cells: How the nuclear exclusion of Cl- affects the plasma membrane potential. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 921-926. PMID: 15637151, PMCID: PMC545567, DOI: 10.1073/pnas.0408597102.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCell NucleusChlorineErythrocytesMembrane PotentialsMicroscopy, FluorescenceNitratesUrodelaConceptsPlasma membrane potentialMembrane potentialExclusion of ClNuclear exclusionCytoplasmic compartmentCellular ClAmphiuma red blood cellsCytoplasmNet negative chargeValues of EmCellsIntracellular ClRed blood cellsBlood cellsCell ClDistribution of ClFluorescent imagingEquilibrium distributionDNANucleusFluorescence intensitySensitive dyeCompartmentsRed cellsNegative charge
2001
Gap Junctional Channels Regulate Acid Secretion in the Mammalian Gastric Gland
Radebold K, Horakova E, Gloeckner J, Ortega G, Spray D, Vieweger H, Siebert K, Manuelidis L, Geibel J. Gap Junctional Channels Regulate Acid Secretion in the Mammalian Gastric Gland. The Journal Of Membrane Biology 2001, 183: 147-153. PMID: 11696856, DOI: 10.1007/s00232-001-0062-9.Peer-Reviewed Original ResearchConceptsGap junction channelsAdjacent parietal cellsCell communicationJunction channelsFunctional gap junction channelsGap junctional channelsPotential physiological roleLucifer yellow transferGap junctional proteinGastric glandsParietal cellsWestern blot analysisJunctional channelsJunctional proteinsPhysiological roleRapid cellBlot analysisCalcium signalsBasolateral membraneCell microinjectionAcid secretory processTransfer of dyeSecretory processIntact glandsCalcium wave propagation
2000
Cell pH and H+ Secretion by S3 Segment of Mammalian Kidney: Role of H+-ATPase and Cl−
Malnic G, Geibel J. Cell pH and H+ Secretion by S3 Segment of Mammalian Kidney: Role of H+-ATPase and Cl−. The Journal Of Membrane Biology 2000, 178: 115-125. PMID: 11083900, DOI: 10.1007/s002320010019.Peer-Reviewed Original ResearchConceptsProximal tubule cellsChannel blocker NPPBS3 segmentTubule cellsProximal S3 segmentsFree mediumCell pHConfocal microscopyNIH Image programCell pH regulationMicroperfusion techniqueSuperfusionMouse kidneyRabbit kidneyKidneySubcellular vesiclesApical areaCell acidificationMammalian kidneyFluorescence densityNPPBRingerCellsImage programNaCl RingerDendrimer-assisted patch-clamp sizing of nuclear pores
Bustamante J, Michelette E, Geibel J, Hanover J, McDonnell T, Dean D. Dendrimer-assisted patch-clamp sizing of nuclear pores. Pflügers Archiv - European Journal Of Physiology 2000, 439: 829-837. PMID: 10784359, PMCID: PMC4397974, DOI: 10.1007/s004249900233.Peer-Reviewed Original ResearchConceptsNuclear pore complexNuclear localization signalMacromolecular translocationFluorescence microscopy dataNuclear envelopeGreen fluorescence proteinLocalization signalPore complexNuclear poresMicroscopy dataColloidal gold particlesFluorescence proteinElectron microscopyCell lysatesFluorescence microscopyPhysiological conditionsDistinct classesCellular structureGold particlesProteinTranslocationUseful probeCardiomyocyte nucleiB-phycoerythrinParticles
1997
Novel Transport Properties of Colonic Crypt Cells: Fluid Absorption and Cl-Dependent Na-H Exchange
Binder H, Singh S, Geibel J, Rajendran V. Novel Transport Properties of Colonic Crypt Cells: Fluid Absorption and Cl-Dependent Na-H Exchange. Comparative Biochemistry And Physiology Part A Molecular & Integrative Physiology 1997, 118: 265-269. PMID: 9366054, DOI: 10.1016/s0300-9629(97)00079-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBody FluidsChloride ChannelsColonIntestinal AbsorptionIon TransportMicroscopy, FluorescenceRatsSodium-Hydrogen ExchangersVideo RecordingConceptsNa-H exchangeCrypt cellsFluid absorptionLamina propria cellsColonic ion transportNet fluid secretionRat distal colonColonic crypt cellsPresence of myofibroblastsApical membrane vesiclesSecretory processRinger-like solutionDibutyryl cyclic AMPDistal colonCrypt preparationNeurohumoral agonistsAcid loadMorphologic techniquesFluid secretionGradient stimulationAbsorptive processesCyclic AMPIndividual cryptsMyofibroblastsBath solutionCyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells
Yano Y, Geibel J, Sumpio B. Cyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells. Journal Of Cellular Biochemistry 1997, 64: 505-513. PMID: 9057108, DOI: 10.1002/(sici)1097-4644(19970301)64:3<505::aid-jcb17>3.0.co;2-e.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsBeta 1 integrinUmbilical vein endothelial cellsVein endothelial cellsEndothelial cellsAlpha 5Beta 1Alpha 5 beta 1Alpha 2 beta 1 integrinH exposureBeta 3 integrinAlpha 2 integrinCycles/minEndothelial cell morphologyVariety of stimuliIntegrin alpha 5Tyrosine phosphorylationVitronectin receptorAlpha 2Redistribution of alphaEC subjectsBeta integrins