2023
Application of a 3D bioprinter: jet technology for ‘biopatch’ development using cells on hydrogel supports
DeMel D, Wagner G, Maresca J, Geibel J. Application of a 3D bioprinter: jet technology for ‘biopatch’ development using cells on hydrogel supports. BioTechniques 2023, 76: 52-62. PMID: 38084384, DOI: 10.2144/btn-2023-0052.Peer-Reviewed Original ResearchThree-Dimensional Bioprinting Applications for Bone Tissue Engineering
Maresca J, DeMel D, Wagner G, Haase C, Geibel J. Three-Dimensional Bioprinting Applications for Bone Tissue Engineering. Cells 2023, 12: 1230. PMID: 37174630, PMCID: PMC10177443, DOI: 10.3390/cells12091230.Peer-Reviewed Original ResearchConceptsBone tissue engineeringThree-dimensional (3D) bioprinting applicationsTissue engineeringSupport structureAdditional support structuresUse of hydrogelsBioprinting applicationsBone replacementNormal bone physiologyQuality of lifeBioprintingKey support structuresBone physiologyInjurySkeletal systemEngineeringMultiple directionsBiological materialsForeign objectsHydrogelsNew techniquePrinterPatientsPathophysiologyDegenerationCRITICALITIES AND USEFULNESS OF EX-VIVO SMALL INTESTINE PERFUSION: TRANSPLANT AND BEYOND
Muñoz-Abraham A, Martinez A, Aviles-Ovalle B, Patron-Lozano R, D’Amico F, Geibel J, Mulligan D, Martins P, Rodriguez-Davalos M. CRITICALITIES AND USEFULNESS OF EX-VIVO SMALL INTESTINE PERFUSION: TRANSPLANT AND BEYOND. European Journal Of Transplantation 2023, 1: 126-135. DOI: 10.57603/ejt-015.Peer-Reviewed Original Research
2022
3D Bioprinting Using Hydrogels: Cell Inks and Tissue Engineering Applications
Dell A, Wagner G, Own J, Geibel J. 3D Bioprinting Using Hydrogels: Cell Inks and Tissue Engineering Applications. Pharmaceutics 2022, 14: 2596. PMID: 36559090, PMCID: PMC9784738, DOI: 10.3390/pharmaceutics14122596.Peer-Reviewed Original ResearchTissue engineering applicationsPrintable formulationsBioprinting processEngineering applicationsProcess optimizationBioprinting methodInk formulationTissue engineeringTailorable materialsBioprintingBiomedical applicationsBiological tissuesHydrogelsNew hydrogelsInkPromising optionNovel methodApplicationsBiological applicationsFormulationVariety of techniquesEngineeringMethodMaterialsL-ARGININE PREVENTS ISCHEMIC INJURY IN EXPLANTED RAT INTESTINAL REGIONS IN AN EX VIVO PERFUSION MODEL
Finotti M, Barahona M, Maina R, Lysyy T, Agarwal R, Schmitt P, Caturegli G, Di Renzo C, Anselmo A, Mulligan D, Geibel J, D'Amico F. L-ARGININE PREVENTS ISCHEMIC INJURY IN EXPLANTED RAT INTESTINAL REGIONS IN AN EX VIVO PERFUSION MODEL. Transplantation Reports 2022, 7: 100096. DOI: 10.1016/j.tpr.2022.100096.Peer-Reviewed Original ResearchIschemic injuryIntestinal segmentsIschemic damageIntraluminal perfusionNitric oxide-arginine pathwayMale Sprague-Dawley ratsN-nitroarginine methyl esterSprague-Dawley ratsEx vivo perfusion modelVivo perfusion modelNon-ischemic conditionsSmall intestinal segmentsFITC-inulinExtraluminal sideL-NAMEReperfusion injuryProtective effectL-arginineSmall intestinePerfusion modelInjuryIntestinal regionsFluid secretionReduced susceptibilityPerfusionColonic Fluid and Electrolyte Transport 2022: An Update
Negussie AB, Dell AC, Davis BA, Geibel JP. Colonic Fluid and Electrolyte Transport 2022: An Update. Cells 2022, 11: 1712. PMID: 35626748, PMCID: PMC9139964, DOI: 10.3390/cells11101712.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane regulatorCell death regulationSignal transduction pathwaysDeath regulationNa-K-Cl cotransporterEpithelial cell deathTransduction pathwaysIon homeostasisRegulatory relationshipsIon transportersTransepithelial ion transportEpithelial sodium channelTransmembrane regulatorCell deathColonic epithelial cellsEpithelial cellsTransportersSodium channelsNa-H exchangerRegulationIon transportCancer pathologyPH modulationIon movementOsmotic gradientGastric secretions
Geibel J. Gastric secretions. 2022, 313-333. DOI: 10.1002/9781119600206.ch17.Peer-Reviewed Original Research
2021
3D Bioprinting of Vascularized Tissues for in vitro and in vivo Applications
Chen EP, Toksoy Z, Davis BA, Geibel JP. 3D Bioprinting of Vascularized Tissues for in vitro and in vivo Applications. Frontiers In Bioengineering And Biotechnology 2021, 9: 664188. PMID: 34055761, PMCID: PMC8158943, DOI: 10.3389/fbioe.2021.664188.Peer-Reviewed Original ResearchThree-dimensional (3D) bioprinting technologyFunctional replacement organsBioprinting technologyTissue engineeringWaste disposalBioprintingVascularized tissuesComplex tissue architectureRelevant applicationsOrgan replacementLatest advancementsComplex vascular networkMaterialsApplicationsReplacement organsCurrent limitationsMain challengesNumerous combinationsTechnologyRapid developmentEngineeringVascular networkVivo applicationsBile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario
D'Amico F, Bertacco A, Finotti M, Di Renzo C, Rodriguez-Davalos MI, Gondolesi GE, Cillo U, Mulligan D, Geibel J. Bile Microbiota in Liver Transplantation: Proof of Concept Using Gene Amplification in a Heterogeneous Clinical Scenario. Frontiers In Surgery 2021, 8: 621525. PMID: 33796547, PMCID: PMC8009296, DOI: 10.3389/fsurg.2021.621525.Peer-Reviewed Original ResearchBile microbiotaLiver donorsBiliary tract diseaseCommon bile ductGene amplificationRibosomal RNA gene amplificationLiver recipientsLiver transplantationPediatric recipientsLiver proceduresBiliary tractBile ductRecipient patientsTract diseaseBile samplesHeterogeneous cohortClinical scenariosBileSterile wayPCR sequencingPatientsRecipientsMicrobiotaTransplantationGallbladder
2020
Hydrogel‐based 3D bioprints repair rat small intestine injuries and integrate into native intestinal tissue
Maina RM, Barahona MJ, Geibel P, Lysyy T, Finotti M, Isaji T, Wengerter B, Mentone S, Dardik A, Geibel JP. Hydrogel‐based 3D bioprints repair rat small intestine injuries and integrate into native intestinal tissue. Journal Of Tissue Engineering And Regenerative Medicine 2020, 15: 129-138. PMID: 33197151, DOI: 10.1002/term.3157.Peer-Reviewed Original ResearchConceptsIntestinal segmentsPost-operative day 7Male Sprague-Dawley ratsMale Wistar ratsSmall intestine injurySprague-Dawley ratsNative intestineBlinded histopathological analysisIntestinal failureStool outputNative intestinal tissueIntestine injuryIntraluminal perfusionRat vascular cellsHistopathological analysisIleocecal junctionWistar ratsIntestinal integrityIntestinal tissueDay 7Small intestineGenetic malformationsNormal activityWeight gainEnterotomyPenicillin G Induces H+, K+-ATPase via a Nitric Oxide-Dependent Mechanism in the Rat Colonic Crypt
Baratta VM, Norz V, Barahona MJ, Gisinger TM, Mulligan D, Geibel JP. Penicillin G Induces H+, K+-ATPase via a Nitric Oxide-Dependent Mechanism in the Rat Colonic Crypt. Cellular Physiology And Biochemistry 2020, 54: 1132-1142. PMID: 33175479, PMCID: PMC8095381, DOI: 10.33594/000000305.Peer-Reviewed Original ResearchConceptsRat colonic cryptsColonic cryptsNitric oxideNitric oxide-dependent mechanismPotassium homeostasisArginine methyl esterAmmonium prepulse techniqueIntracellular nitric oxideSodium-free conditionsL-NAMEDiarrheal statesATPase activityBACKGROUND/Presence of NOPenicillin G sodium saltAcute exposureL-arginineSmall intestineIsolated administrationN-nitroHomeostatic conditionsCryptsPrepulse techniqueSignificant reductionNovel mechanismHuman Small Intestine Transplantation: Segmental Susceptibility to Ischemia Using Different Preservation Solutions and Conditions
Lysyy T, Finotti M, Maina RM, Morotti R, Munoz-Abraham AS, Bertacco A, Ibarra C, Barahona M, Agarwal R, D'Amico F, Rodriguez-Davalos MI, Mulligan D, Geibel J. Human Small Intestine Transplantation: Segmental Susceptibility to Ischemia Using Different Preservation Solutions and Conditions. Transplantation Proceedings 2020, 52: 2934-2940. PMID: 32768284, DOI: 10.1016/j.transproceed.2020.06.031.Peer-Reviewed Original ResearchConceptsContinuous perfusion preservationIntestinal graftIschemic damagePerfusion preservationAppropriate graft selectionCold static storageSmall intestinal graftsSmall intestine graftDifferent preservation solutionsBlinded histological evaluationCross clampIntestine graftsPostoperative complicationsDifferent time pointsIschemic injuryDeceased donorsPatient survivalGraft selectionRinger's lactateHistological evaluationBack tableVascular perfusionLuminal flushAbdominal organsSmall intestine
2019
In Pursuit of the Parietal Cell – An Evolution of Scientific Methodology and Techniques
Baratta V, Own J, Di Renzo C, Ollodart J, Geibel JP, Barahona M. In Pursuit of the Parietal Cell – An Evolution of Scientific Methodology and Techniques. Frontiers In Physiology 2019, 10: 1497. PMID: 31920702, PMCID: PMC6920182, DOI: 10.3389/fphys.2019.01497.Peer-Reviewed Original ResearchInduction of Secretagogue Independent Gastric Acid Secretion via a Novel Aspirin-Activated Pathway
Kitay AM, Ferstl FS, Link A, Geibel JP. Induction of Secretagogue Independent Gastric Acid Secretion via a Novel Aspirin-Activated Pathway. Frontiers In Physiology 2019, 10: 1264. PMID: 31649553, PMCID: PMC6795678, DOI: 10.3389/fphys.2019.01264.Peer-Reviewed Original ResearchGastric acid secretionNon-selective NOS inhibitor L-NAMENOS inhibitor L-NAMEInhibitor L-NAMEAcid secretionL-NAMESoluble guanylyl cyclase inhibitor 1HGuanylyl cyclase inhibitor 1HIncreases nitric oxide productionUsage of aspirinGastric glandsPH-sensitive dye BCECF-AMNitric oxide productionVariety of cancersNovel secretagogueBlood clot formationAspirin exposureChronic conditionsMucosal barrierRepetitive dosesUlcer formationAspirin consumptionBasolateral exposureAspirinClot formationThe calcium‐sensing receptor: A novel target for treatment and prophylaxis of neratinib‐induced diarrhea
Lysyy T, Lalani AS, Olek EA, Diala I, Geibel JP. The calcium‐sensing receptor: A novel target for treatment and prophylaxis of neratinib‐induced diarrhea. Pharmacology Research & Perspectives 2019, 7: e00521. PMID: 31523434, PMCID: PMC6743423, DOI: 10.1002/prp2.521.Peer-Reviewed Original ResearchConceptsTyrosine kinase inhibitorsCaSR activationCalcium-sensing receptorDose-dependent mannerFluid secretionRat intestineHER2-positive breast cancerKinase inhibitorsAdverse effectsChemotherapy-associated diarrheaNeratinib-induced diarrheaCommon side effectsIsolated intestinal segmentsPotent therapeutic targetIrreversible panPatients' qualityBreast cancerIntestinal segmentsSide effectsTherapeutic targetDiarrheaPronounced elevationIntraluminal calcium concentrationVivo modelNeratinibActivation of the Calcium Sensing Receptor Decreases Secretagogue-Induced Fluid Secretion in the Rat Small Intestine
Barahona MJ, Maina RM, Lysyy T, Finotti M, Caturegli G, Baratta V, D’Amico F, Mulligan D, Geibel JP. Activation of the Calcium Sensing Receptor Decreases Secretagogue-Induced Fluid Secretion in the Rat Small Intestine. Frontiers In Physiology 2019, 10: 439. PMID: 31130866, PMCID: PMC6509940, DOI: 10.3389/fphys.2019.00439.Peer-Reviewed Original ResearchPresence of forskolinFluid secretionSmall intestineAC-265347Fluid absorptionIntestinal fluid secretionCalcium-sensing receptorSprague-Dawley ratsNew therapeutic targetsNet fluid absorptionDose-dependent mannerRat small intestineMM CaAbsence of forskolinMammalian gastrointestinal tractFluid hypersecretionDiarrheal illnessIntraluminal perfusionPotent secretagogueDawley ratsGastrointestinal tractCaSR activatorsElectrolyte balanceTherapeutic targetLuminal perfusateMo1102 – Aspirin and Ischemia in the Murine Distal Colon
Baratta V, Barahona M, Mulligan D, Geibel J. Mo1102 – Aspirin and Ischemia in the Murine Distal Colon. Gastroenterology 2019, 156: s-708. DOI: 10.1016/s0016-5085(19)38700-1.Peer-Reviewed Original ResearchSa1745 – Glacial Acetic Acid-Induced Ulcerative Colitis Lesions Improve After Oral Calcium Nanoparticle Therapy in a Rat Model
Barahona M, Baratta V, Mulligan D, Geibel J. Sa1745 – Glacial Acetic Acid-Induced Ulcerative Colitis Lesions Improve After Oral Calcium Nanoparticle Therapy in a Rat Model. Gastroenterology 2019, 156: s-384. DOI: 10.1016/s0016-5085(19)37812-6.Peer-Reviewed Original ResearchSa1746 – Oral Calcium Nanoparticle Therapy Aids in the Recovery from Dss-Induced Ulcerative Colitis in the Rat Model
Baratta V, Barahona M, Mulligan D, Geibel J. Sa1746 – Oral Calcium Nanoparticle Therapy Aids in the Recovery from Dss-Induced Ulcerative Colitis in the Rat Model. Gastroenterology 2019, 156: s-384-s-385. DOI: 10.1016/s0016-5085(19)37813-8.Peer-Reviewed Original ResearchDesign and implementation of novel nutraceuticals and derivatives for treating intestinal disorders
Barahona MJ, Baratta V, Ollodart J, Mulligan D, Geibel JP. Design and implementation of novel nutraceuticals and derivatives for treating intestinal disorders. Future Medicinal Chemistry 2019, 11: 847-855. PMID: 30994367, PMCID: PMC8008207, DOI: 10.4155/fmc-2018-0313.Peer-Reviewed Original ResearchConceptsSignificant worldwide disease burdenSpectrum of illnessWorldwide disease burdenIntestinal fluid secretionSystemic side effectsCalcium-sensing receptorNovel therapeutic targetG protein-coupled receptorsSurgical therapyPharmaceutical optionsMucosal integrityDisease burdenIntestinal disordersNutraceutical therapyGastrointestinal diseasesGastrointestinal illnessSide effectsTherapeutic targetFluid secretionIntestinal cellsTherapyAdverse effectsNovel nutraceuticalsCaSRIllness