2000
Development and characterization of antibodies specific to caspase-3-produced alpha II-spectrin 120 kDa breakdown product: marker for neuronal apoptosis
Nath R, Huggins M, Glantz S, Morrow J, McGinnis K, Nadimpalli R, Wang K. Development and characterization of antibodies specific to caspase-3-produced alpha II-spectrin 120 kDa breakdown product: marker for neuronal apoptosis. Neurochemistry International 2000, 37: 351-361. PMID: 10825575, DOI: 10.1016/s0197-0186(00)00040-1.Peer-Reviewed Original ResearchConceptsWestern blotRat cerebellar granule neuronsHuman neuroblastoma SH-SY5Y cellsNeuroblastoma SH-SY5Y cellsSpectrin breakdown productsCerebellar granule neuronsSH-SY5Y cellsApoptotic neuronsCharacterization of antibodiesNeuronal apoptosisNeurodegenerative conditionsGranule neuronsBreakdown productsImmunocytochemical studySH-SY5YII-spectrinWithdrawal-induced apoptosisAntibodiesNeuronsCaspase-3Apoptotic deathPowerful markerChicken antibodiesApoptosisAlpha-spectrin
1988
Comparison of nonerythroid alpha-spectrin genes reveals strict homology among diverse species.
Leto T, Fortugno-Erikson D, Barton D, Yang-Feng T, Francke U, Harris A, Morrow J, Marchesi V, Benz E. Comparison of nonerythroid alpha-spectrin genes reveals strict homology among diverse species. Molecular And Cellular Biology 1988, 8: 1-9. PMID: 3336352, PMCID: PMC363070, DOI: 10.1128/mcb.8.1.1.Peer-Reviewed Original ResearchConceptsAlpha-spectrin geneErythroid alpha-spectrin geneHuman chromosome 9Alpha-spectrin chainComparison of sequencesCarboxy-terminal sequenceErythroid genesDiverse speciesExpression libraryChromosome 1Hydrophobic residuesErythroid spectrinAlpha-spectrinFilamentous proteinsFunctional sitesGenesChromosome 9Human cloneStrict homologyRepeat patternCell membraneInvariant tryptophanSpectrinPeptide sequencesCDNA