1994
cAMP and tumor necrosis factor competitively regulate transcriptional activation through and nuclear factor binding to the cAMP-responsive element/activating transcription factor element of the endothelial leukocyte adhesion molecule-1 (E-selectin) promoter.
De Luca LG, Johnson DR, Whitley MZ, Collins T, Pober JS. cAMP and tumor necrosis factor competitively regulate transcriptional activation through and nuclear factor binding to the cAMP-responsive element/activating transcription factor element of the endothelial leukocyte adhesion molecule-1 (E-selectin) promoter. Journal Of Biological Chemistry 1994, 269: 19193-19196. PMID: 7518452, DOI: 10.1016/s0021-9258(17)32150-6.Peer-Reviewed Original ResearchConceptsConsensus cAMP-responsive elementCRE-binding proteinTranscription factor elementsTranscriptional activationCRE/ATF elementElectrophoretic mobility shift assaysMobility shift assaysTransient transfection assaysAntibody supershift assaysCAMP-responsive elementMigrating formPromoter elementsDNA sequencesFastest migrating formBovine aortic endothelial cellsShift assaysTransfection assaysPromoter responseSupershift assaysGene expressionFactor elementsC-JunAortic endothelial cellsEffects of TNFProtein
1987
Effects of Tumour Necrosis Factor and Related Cytokines on Vascular Endothelial Cells
Pober J. Effects of Tumour Necrosis Factor and Related Cytokines on Vascular Endothelial Cells. Novartis Foundation Symposia 1987, 131: 170-191. PMID: 3330009, DOI: 10.1002/9780470513521.ch12.Peer-Reviewed Original ResearchConceptsIL-1 betaHuman endothelial cellsIL-1 alphaRelated cytokinesVascular endothelial cellsEndothelial cellsNecrosis factorEndothelial leucocyte adhesion moleculeIntercellular adhesion molecule-1Tumor necrosis factorInterleukin-1 betaAdhesion molecule-1Endothelial cell activationInterleukin-1 alphaCultured human endothelial cellsPhorbol esterEffects of TNFRecombinant human TNFCytoplasmic calcium concentrationIL-1 speciesMechanism of actionHEC adhesivenessInflammatory leucocytesCell surface expressionProtein kinase C pathway
1986
Recombinant tumor necrosis factor induces procoagulant activity in cultured human vascular endothelium: characterization and comparison with the actions of interleukin 1.
Bevilacqua MP, Pober JS, Majeau GR, Fiers W, Cotran RS, Gimbrone MA. Recombinant tumor necrosis factor induces procoagulant activity in cultured human vascular endothelium: characterization and comparison with the actions of interleukin 1. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 4533-4537. PMID: 3487091, PMCID: PMC323768, DOI: 10.1073/pnas.83.12.4533.Peer-Reviewed Original ResearchConceptsRecombinant tumor necrosis factorCultured human vascular endotheliumTumor necrosis factorIL-1Procoagulant activityHuman vascular endotheliumNecrosis factorInterleukin-1Vascular endotheliumTissue factor-like procoagulant activityInduction of PCANatural human interleukin-1Human recombinant tumor necrosis factorEndothelial cell damageCellular procoagulant activityEndothelial procoagulant activityRecombinant IL-1Specific neutralizing antiseraHuman interleukin-1Effects of TNFInitiation of coagulationIliac arteryMaximal dosesPCA responseSaphenous vein