2006
Endothelial Cell Dysfunction, Injury and Death
Pober JS, Min W. Endothelial Cell Dysfunction, Injury and Death. Handbook Of Experimental Pharmacology 2006, 176/II: 135-156. PMID: 16999227, DOI: 10.1007/3-540-36028-x_5.Peer-Reviewed Original ResearchConceptsTumor necrosis factorCytolytic T lymphocytesEC injuryEndothelial cellsReactive oxygen speciesEndothelial cell dysfunctionMacrophage-derived cytokinesVascular endothelial cellsEndothelial dysfunctionNecrosis factorCell dysfunctionT lymphocytesInjuryCommon mediatorInflammationDysfunctionDeathOxygen speciesCytokinesLymphocytesNeutrophilsDifferent agentsBiochemical pathways
2003
Combining altered levels of effector transcripts in circulating T cells with a marker of endothelial injury is specific for active graft-versus-host disease
Biedermann BC, Tsakiris DA, Gregor M, Pober JS, Gratwohl A. Combining altered levels of effector transcripts in circulating T cells with a marker of endothelial injury is specific for active graft-versus-host disease. Bone Marrow Transplantation 2003, 32: 1077-1084. PMID: 14625579, DOI: 10.1038/sj.bmt.1704258.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkersCase-Control StudiesEndothelium, VascularFas Ligand ProteinGraft vs Host DiseaseHematopoietic Stem Cell TransplantationInterferon-gammaLymphocyte ActivationMembrane GlycoproteinsMolecular Diagnostic TechniquesRNA, MessengerSensitivity and SpecificitySurvivorsT-Lymphocytes, CytotoxicTransplantation, HomologousVon Willebrand FactorConceptsCytotoxic T lymphocytesEndothelial injuryT cell activationT cellsT lymphocytesHost diseaseSCT recipientsAllogeneic stem cell transplantationAllospecific cytotoxic T lymphocytesIFN-γ transcript levelsActive chronic GVHDEndothelial injury resultsCD8 T lymphocytesCD8 T cellsCD4 T cellsLong-term survivorsStem cell transplantationImportant effector cellsVon Willebrand factor plasma levelsAge-matched controlsVascular endothelial cellsChronic GVHDActive graftEffector cellsCell transplantation
2000
Human Vascular Endothelial Cells Stimulate Memory But Not Naive CD8+ T Cells to Differentiate into CTL Retaining an Early Activation Phenotype
Dengler T, Pober J. Human Vascular Endothelial Cells Stimulate Memory But Not Naive CD8+ T Cells to Differentiate into CTL Retaining an Early Activation Phenotype. The Journal Of Immunology 2000, 164: 5146-5155. PMID: 10799873, DOI: 10.4049/jimmunol.164.10.5146.Peer-Reviewed Original ResearchMeSH KeywordsB-LymphocytesCD8-Positive T-LymphocytesCell DifferentiationCells, CulturedCoculture TechniquesCyclosporineCytotoxicity Tests, ImmunologicEndothelium, VascularHistocompatibility Antigens Class IHumansImmunologic MemoryImmunophenotypingInterleukin-12InterphaseLeukocyte Common AntigensLymphocyte ActivationT-Lymphocyte SubsetsT-Lymphocytes, CytotoxicConceptsB lymphoblastoid cellsT cellsNaive CD8Endothelial cellsImmunoregulatory cell typesIntracellular perforin contentAlloreactive T cellsAnti-CD28 mAbHuman vascular endothelial cellsHigh surface expressionVascular endothelial cellsExpansion of memoryConventional CTLGraft parenchymaGraft rejectionMemory CD8CTL generationPerforin contentCTL expansionVascular injuryHuman CD8CD40 ligandAlloreactive CTLAnatomic compartmentsICAM-1Cytoprotection of Human Umbilical Vein Endothelial Cells Against Apoptosis and CTL-Mediated Lysis Provided by Caspase-Resistant Bcl-2 Without Alterations in Growth or Activation Responses
Zheng L, Dengler T, Kluger M, Madge L, Schechner J, Maher S, Pober J, Bothwell A. Cytoprotection of Human Umbilical Vein Endothelial Cells Against Apoptosis and CTL-Mediated Lysis Provided by Caspase-Resistant Bcl-2 Without Alterations in Growth or Activation Responses. The Journal Of Immunology 2000, 164: 4665-4671. PMID: 10779771, DOI: 10.4049/jimmunol.164.9.4665.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisCaspasesCell DivisionCell Line, TransformedCells, CulturedCulture Media, ConditionedCytotoxicity, ImmunologicEndothelial Growth FactorsEndothelium, VascularGenetic VectorsGreen Fluorescent ProteinsHumansLuminescent ProteinsProto-Oncogene Proteins c-bcl-2RetroviridaeT-Lymphocytes, CytotoxicTransduction, GeneticTransfectionUmbilical VeinsConceptsGraft endothelial cellsAllograft rejectionBcl-2Endothelial cellsAcute allograft rejectionClass I MHC moleculesNF-kappaB activationHuman umbilical vein endothelial cellsI MHC moleculesUmbilical vein endothelial cellsHost CTLVein endothelial cellsEndothelial injuryAnti-apoptotic gene Bcl-2MHC moleculesGene Bcl-2Induction of apoptosisBcl-2-transduced cellsClass IActivation responseApoptotic effectsCTLHUVECTNFGrowth factor withdrawalIL-11 Activates Human Endothelial Cells to Resist Immune-Mediated Injury
Mahboubi K, Biedermann B, Carroll J, Pober J. IL-11 Activates Human Endothelial Cells to Resist Immune-Mediated Injury. The Journal Of Immunology 2000, 164: 3837-3846. PMID: 10725745, DOI: 10.4049/jimmunol.164.7.3837.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDCells, CulturedComplement System ProteinsCytokine Receptor gp130Cytotoxicity, ImmunologicDNA-Binding ProteinsDose-Response Relationship, ImmunologicEndothelium, VascularEnzyme ActivationHumansImmunity, InnateInflammation MediatorsInterleukin-11Interleukin-11 Receptor alpha SubunitMembrane GlycoproteinsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesNF-kappa BPhosphorylationReceptors, InterleukinReceptors, Interleukin-11Signal TransductionSTAT1 Transcription FactorSTAT3 Transcription FactorT-Lymphocytes, CytotoxicTrans-ActivatorsTyrosineUmbilical VeinsConceptsIL-11Mitogen-activated protein kinaseP44 mitogen-activated protein kinaseImmune-Mediated InjuryCytolytic T lymphocytesNF-kappaB activationGp130-signaling cytokinesInflammatory injuryHuman endothelial cellsIL-11 receptorProinflammatory responseMolecule expressionT lymphocytesICAM-1Maximal responseE-selectinMHC AbsVivo modelNF-kappaBEndothelial cellsTyrosine phosphorylationPhospho-STAT3Cultured HUVECsInjuryKinase 1 inhibitor
1999
Human vascular endothelial cells favor clonal expansion of unusual alloreactive CTL.
Biedermann B, Pober J. Human vascular endothelial cells favor clonal expansion of unusual alloreactive CTL. The Journal Of Immunology 1999, 162: 7022-30. PMID: 10358144, DOI: 10.4049/jimmunol.162.12.7022.Peer-Reviewed Original ResearchConceptsTarget cell contactB lymphoblastoid cellsLittle IFN-gammaIFN-gammaEndothelial cellsCTL clonesClass I MHC-restricted CTLAbsence of ECExpression of CD40LAllogeneic endothelial cellsIFN-gamma secretionIL-4 secretionCell contactHuman vascular endothelial cellsVascular endothelial cellsCTL subsetsCD40L expressionCTL linesCD40 ligandT cellsAlloreactive CTLArterial intimaClonal expansionCTLCultured HUVECs
1998
Human endothelial cells induce and regulate cytolytic T cell differentiation.
Biedermann B, Pober J. Human endothelial cells induce and regulate cytolytic T cell differentiation. The Journal Of Immunology 1998, 161: 4679-87. PMID: 9794397, DOI: 10.4049/jimmunol.161.9.4679.Peer-Reviewed Original ResearchConceptsB lymphoblastoid cellsEndothelial cellsHuman endothelial cellsSame donorT cellsProliferation of CD8IFN-gamma secretionCultured human endothelial cellsT cell differentiationT cell growthAllogeneic CD8CTL responsesPerforin expressionCTL differentiationPresence of ECT lymphocytesAllospecific CTLCD8CTLEfficient stimulatorsLymphoblastoid cellsCell numberReproducible frequencyCell growthInput cell number
1984
Human cytolytic T lymphocyte interactions with vascular endothelium and fibroblasts: role of effector and target cell molecules.
Collins T, Krensky AM, Clayberger C, Fiers W, Gimbrone MA, Burakoff SJ, Pober JS. Human cytolytic T lymphocyte interactions with vascular endothelium and fibroblasts: role of effector and target cell molecules. The Journal Of Immunology 1984, 133: 1878-84. PMID: 6432903, DOI: 10.4049/jimmunol.133.4.1878.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntigens, SurfaceBinding Sites, AntibodyCell CommunicationCytotoxicity, ImmunologicEndotheliumFibroblastsHistocompatibility Antigens Class IIHLA-DR AntigensHumansIndiumInterferon-gammaLymphocyte Function-Associated Antigen-1RadioisotopesT-LymphocytesT-Lymphocytes, CytotoxicUmbilical VeinsConceptsCytolytic T lymphocytesVascular endotheliumCultured human vascular endotheliumLFA-1LFA-2Human cytolytic T lymphocytesHuman CTL linesT lymphocyte interactionsCell typesSame CTL clonesImportant target cellsTarget cell structuresHuman vascular endotheliumLymphoid targetsHost diseaseAllograft rejectionImmune injuryHLA-DRCell surface moleculesCTL linesCTL clonesT lymphocytesLymphocyte interactionsTarget cell typeLFA-3