2008
Modulation of the actin cytoskeleton by Salmonella
Galan J. Modulation of the actin cytoskeleton by Salmonella. The FASEB Journal 2008, 22: 530.1-530.1. DOI: 10.1096/fasebj.22.1_supplement.530.1.Peer-Reviewed Original ResearchEffector proteinsHost cellsActin cytoskeletonBacterial proteinsCellular responsesBacterial effector proteinsProtein secretion systemSpecialized protein secretion systemActin cytoskeletal reorganizationDownstream effector proteinsSignal transduction pathwaysProtein Cdc42Exchange factorMembrane rufflingSmall GTPRho GTPasesSecretion systemCytoskeletal reorganizationTransduction pathwaysCytoskeletal rearrangementsCdc42RhoG.ProteinCytoskeletonRac
2000
Striking a balance: Modulation of the actin cytoskeleton by Salmonella
Galán J, Zhou D. Striking a balance: Modulation of the actin cytoskeleton by Salmonella. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 8754-8761. PMID: 10922031, PMCID: PMC34008, DOI: 10.1073/pnas.97.16.8754.Peer-Reviewed Original ResearchConceptsType III secretion systemSecretion systemHost cellsBacterial entryGTP-binding protein Cdc42Bacterial effector proteinsInositol polyphosphate phosphatasePathogen/host relationshipActin cytoskeleton rearrangementHost cell cytosolStimulation of Cdc42Protein Cdc42Effector proteinsActin cytoskeletonExchange factorSpecialized organellesSalmonella proteinsCytoskeleton rearrangementT-plastinHost relationshipsCell cytosolF-actinCellular responsesG proteinsCdc42
1999
A Salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasion
Fu Y, Galán J. A Salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasion. Nature 1999, 401: 293-297. PMID: 10499590, DOI: 10.1038/45829.Peer-Reviewed Original ResearchMeSH KeywordsActinsArginineBacterial AdhesionBacterial ProteinsCdc42 GTP-Binding Protein, Saccharomyces cerevisiaeCell Cycle ProteinsCell MembraneEscherichia coliGTPase-Activating ProteinsGTP-Binding ProteinsHumansJNK Mitogen-Activated Protein KinasesMAP Kinase Kinase 4Mitogen-Activated Protein Kinase KinasesMutationProtein KinasesProtein Tyrosine PhosphatasesProteinsRecombinant Fusion ProteinsSalmonella typhimuriumConceptsHost cell cytosolActin cytoskeletonType III secretion systemProtein secretion systemSpecialized protein secretion systemActin cytoskeleton reorganizationCell actin cytoskeletonActin cytoskeletal changesRho GTPase proteinsRac-1Bacterial effectorsEffector proteinsExchange factorGTPase proteinsSecretion systemSalmonella proteinsCytoskeletal changesCellular responsesCdc42ProteinInfected cellsBacterial invasionCytosolBacteriumSPTPAn invasion-associated Salmonella protein modulates the actin-bundling activity of plastin
Zhou D, Mooseker M, Galán J. An invasion-associated Salmonella protein modulates the actin-bundling activity of plastin. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 10176-10181. PMID: 10468582, PMCID: PMC17862, DOI: 10.1073/pnas.96.18.10176.Peer-Reviewed Original ResearchConceptsActin-bundling activityT-plastinBacterial-host cell contactHost cell signal transduction pathwaysHost cellsType III secretion systemBacterial effector proteinsActin-binding proteinsSignal transduction pathwaysBacterial effectorsMembrane rufflesEffector proteinsActin cytoskeletonMembrane rufflingSecretion systemSalmonella proteinsBacterial entryBacterial internalizationSalmonella entrySignaling processesActin filamentsF-actinNonphagocytic cellsProteinCell contactRole of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization
Zhou D, Mooseker M, Galán J. Role of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization. Science 1999, 283: 2092-2095. PMID: 10092234, DOI: 10.1126/science.283.5410.2092.Peer-Reviewed Original ResearchInteraction of Salmonella spp. with host cells
Murli S, Galán J. Interaction of Salmonella spp. with host cells. Advances In Cellular And Molecular Biology Of Membranes And Organelles 1999, 6: 173-180. DOI: 10.1016/s1874-5172(99)80011-x.Peer-Reviewed Original ResearchEffector proteinsHost cellsBacterial effector proteinsHost cell cytosolSignal transduction pathwaysHost cell responsesFacultative intracellular pathogenActin cytoskeletonMembrane rufflingTranscription factorsTransduction pathwaysElaborate mechanismsCell cytosolSubsequent translocationMicrobial pathogensCell deathHost environmentCell typesIntracellular pathogensDistinct responsesSalmonella entericaPathogensHostProteinIntimate interaction
1998
Identification of a Specific Chaperone for SptP, a Substrate of the Centisome 63 Type III Secretion System ofSalmonella typhimurium
Fu Y, Galán J. Identification of a Specific Chaperone for SptP, a Substrate of the Centisome 63 Type III Secretion System ofSalmonella typhimurium. Journal Of Bacteriology 1998, 180: 3393-3399. PMID: 9642193, PMCID: PMC107295, DOI: 10.1128/jb.180.13.3393-3399.1998.Peer-Reviewed Original ResearchMeSH KeywordsActinsAmino Acid SequenceBacterial ProteinsBase SequenceChromosomes, BacterialCloning, MolecularComplement Inactivator ProteinsCytoskeletonKineticsMolecular Sequence DataPlasmidsPolymerase Chain ReactionProtein Tyrosine PhosphatasesRecombinant Fusion ProteinsSalmonella typhimuriumSequence AlignmentSequence Homology, Amino AcidConceptsType III secretion systemSecretion systemSpecific chaperonesActin cytoskeletonTyrosine phosphataseType III protein secretion systemHost cell actin cytoskeletonHost cellsProtein secretion systemCell actin cytoskeletonHost cell responsesPulse-chase experimentsCytoplasmic chaperonesCentisome 63Effector proteinsTranscription factorsAcidic polypeptidesChaperonesResidues 15Function mutationsEffector moleculesCytoskeletonProteinSPTPSalmonella typhimuriumThe Salmonella typhimurium tyrosine phosphatase SptP is translocated into host cells and disrupts the actin cytoskeleton
Fu Y, Galán J. The Salmonella typhimurium tyrosine phosphatase SptP is translocated into host cells and disrupts the actin cytoskeleton. Molecular Microbiology 1998, 27: 359-368. PMID: 9484891, DOI: 10.1046/j.1365-2958.1998.00684.x.Peer-Reviewed Original ResearchConceptsActin cytoskeletonHost cell actin cytoskeletonFusion proteinHost cellsAmino acidsAmino-terminal halfCell actin cytoskeletonCultured cells resultsWild-type toxinCultured epithelial cellsEffector proteinsModular domainsStress fibersActive GSTCytoskeletonProteinCells resultsEpithelial cellsGSTSPTPProlonged incubationTranslocationCellsS. typhimuriumMicroinjection