2001
A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalization
Zhou D, Chen L, Hernandez L, Shears S, Galán J. A Salmonella inositol polyphosphatase acts in conjunction with other bacterial effectors to promote host cell actin cytoskeleton rearrangements and bacterial internalization. Molecular Microbiology 2001, 39: 248-260. PMID: 11136447, DOI: 10.1046/j.1365-2958.2001.02230.x.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBacterial ProteinsCdc42 GTP-Binding ProteinCell MembraneCells, CulturedChlorocebus aethiopsCOS CellsCytoskeletonHumansInositol PhosphatesIntestinesJNK Mitogen-Activated Protein KinasesMAP Kinase Kinase 4Mitogen-Activated Protein Kinase KinasesPhosphoric Monoester HydrolasesPhosphorylationSalmonella InfectionsSalmonella typhimuriumTransfectionConceptsActin cytoskeleton rearrangementCytoskeleton rearrangementBacterial entrySecretion systemBacterial internalizationCellular responsesHost cellsRho GTPases signalingProtein secretion systemHost cell actin cytoskeleton rearrangementsHost cellular functionsSpecialized protein secretion systemCdc42-dependent mannerNon-phagocytic cellsBacterial effectorsAbility of SalmonellaInositol phosphataseCellular functionsDefective mutantsBacterial proteinsCo-ordinated functionSalmonella pathogenicityBacterium's abilitySopBPhospholipase C.
2000
Biophysical characterization of SipA, an actin‐binding protein from Salmonella enterica
Mitra K, Zhou D, Galán J. Biophysical characterization of SipA, an actin‐binding protein from Salmonella enterica. FEBS Letters 2000, 482: 81-84. PMID: 11018527, DOI: 10.1016/s0014-5793(00)02040-8.Peer-Reviewed Original ResearchConceptsActin dynamicsCoiled-coil domainF-actin stabilityActin-binding proteinsActin cytoskeleton reorganizationNon-phagocytic cellsBacterial entryCytoskeleton reorganizationAdjacent actin monomersC-terminal fragmentBiophysical characterizationF-actinCellular responsesHost cellsBacterial uptakeActin monomersProteinSalmonella entericaHelical conformationSalmonella enterica infectionsIntestinal epitheliumEnterica infectionsEssential stepSIPACells
1999
Characterization of SprA, an AraC‐like transcriptional regulator encoded within the Salmonella typhimurium pathogenicity island 1
Eichelberg K, Hardt W, Galán J. Characterization of SprA, an AraC‐like transcriptional regulator encoded within the Salmonella typhimurium pathogenicity island 1. Molecular Microbiology 1999, 33: 139-152. PMID: 10411731, DOI: 10.1046/j.1365-2958.1999.01458.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAraC Transcription FactorBacterial ProteinsBiological TransportCells, CulturedChromosome MappingChromosomes, BacterialConsensus SequenceDNA-Binding ProteinsEpithelial CellsGene Expression Regulation, BacterialGenes, BacterialGenes, ReporterHelix-Loop-Helix MotifsMolecular Sequence DataMultigene FamilyPhylogenyRecombinant Fusion ProteinsRepressor ProteinsSalmonella typhimuriumSequence AlignmentSequence Homology, Amino AcidSignal TransductionTrans-ActivatorsTranscription FactorsTranscription, GeneticVirulenceConceptsType III secretion systemTranscriptional regulatory proteinsPathogenicity island 1Secretion systemRegulatory proteinsType III protein secretion systemSPI-1Island 1Host cellsProtein secretion systemTranscriptional regulatory cascadeSpecific transcriptional regulatory proteinsAdditional regulatory proteinsSignificant sequence similarityAraC-like transcriptional regulatorActin cytoskeletal rearrangementRegulation of expressionInvasion-associated genesExpression of genesNon-phagocytic cellsCentisome 63Effector proteinsTranscriptional regulatorsRegulatory cascadeSequence similarity
1997
The invasion-associated type-III protein secretion system inSalmonella – a review1Presented at the Workshop on `Type-4 Pili – Biogenesis, Adhesins, Protein Export, and DNA Import', Schloss Ringberg, Germany, 26–29 November 1995.1
Collazo C, Galán J. The invasion-associated type-III protein secretion system inSalmonella – a review1Presented at the Workshop on `Type-4 Pili – Biogenesis, Adhesins, Protein Export, and DNA Import', Schloss Ringberg, Germany, 26–29 November 1995.1. Gene 1997, 192: 51-59. PMID: 9224874, DOI: 10.1016/s0378-1119(96)00825-6.Peer-Reviewed Original ResearchConceptsProtein secretion systemSecretion systemType-4 Pili – BiogenesisHost cellsEukaryotic host cellsSpecialized protein secretion systemNon-phagocytic cellsMolecular genetic analysisDNA importPili biogenesisProtein exportSchloss RingbergCentisome 63Bacterial chromosomeAnimal pathogensGenetic analysisPathogenicity islandGenetic determinantsCellsReview1PresentedChromosomesPlantsProteinTranslocationAdhesins
1996
Salmonella spp. are cytotoxic for cultured macrophages
Chen L, Kaniga K, Galán J. Salmonella spp. are cytotoxic for cultured macrophages. Molecular Microbiology 1996, 21: 1101-1115. PMID: 8885278, DOI: 10.1046/j.1365-2958.1996.471410.x.Peer-Reviewed Original ResearchConceptsProtein secretion systemNon-phagocytic cellsBacterial internalizationType III protein secretion systemCell deathFragmentation of chromatinWild-type Salmonella typhimuriumMacrophage cell deathBone marrow-derived murine macrophagesMurine macrophagesFeatures of apoptosisCentisome 63Membrane rufflingSecretion apparatusMembrane blebbingBacterial entrySalmonella chromosomeMacrophage cytotoxicityHost rangeCytoplasmic nucleosomesApoptotic bodiesBacterial uptakeCytochalasin DBiochemical techniquesMutationsMolecular genetic bases of Salmonella entry into host cells
Galán J. Molecular genetic bases of Salmonella entry into host cells. Molecular Microbiology 1996, 20: 263-271. PMID: 8733226, DOI: 10.1111/j.1365-2958.1996.tb02615.x.Peer-Reviewed Original ResearchConceptsMolecular genetic basisGenetic basisContact-dependent secretion systemsPlant pathogenic bacteriaNumber of proteinsNon-phagocytic cellsCultured epithelial cellsCentisome 63Secretion systemVariety of animalsKb regionSalmonella chromosomeSalmonella entryExtracellular environmentRemarkable homologyHost cellsEpithelial cellsEntry functionCellsChromosomesConsiderable progressHomologyLociOrganismsProtein
1994
Interactions of bacteria with non-phagocytic cells
Galan J. Interactions of bacteria with non-phagocytic cells. Current Opinion In Immunology 1994, 6: 590-595. PMID: 7946047, DOI: 10.1016/0952-7915(94)90146-5.Peer-Reviewed Original Research