2006
Role of the caspase-1 inflammasome in Salmonella typhimurium pathogenesis
Lara-Tejero M, Sutterwala FS, Ogura Y, Grant EP, Bertin J, Coyle AJ, Flavell RA, Galán J. Role of the caspase-1 inflammasome in Salmonella typhimurium pathogenesis. Journal Of Experimental Medicine 2006, 203: 1407-1412. PMID: 16717117, PMCID: PMC2118315, DOI: 10.1084/jem.20060206.Peer-Reviewed Original Research
2001
Induction of specific CD8+ memory T cells and long lasting protection following immunization with Salmonella typhimurium expressing a lymphocytic choriomeningitis MHC class I-restricted epitope
Shams H, Poblete F, Rüssmann H, Galán J, Donis R. Induction of specific CD8+ memory T cells and long lasting protection following immunization with Salmonella typhimurium expressing a lymphocytic choriomeningitis MHC class I-restricted epitope. Vaccine 2001, 20: 577-585. PMID: 11672924, DOI: 10.1016/s0264-410x(01)00363-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen-Presenting CellsCD8-Positive T-LymphocytesCell LineEpitopes, T-LymphocyteFemaleHistocompatibility Antigens Class IImmunizationImmunologic MemoryLymphocytic choriomeningitis virusMiceMice, Inbred BALB CMice, Inbred C57BLSalmonella typhimuriumVaccines, SyntheticViral VaccinesConceptsMHC class ISurvival of miceProtective immunityClass ICTL responsesT cellsAntiviral CTL responsesProtective CTL responsesMemory T cellsLymphocytic choriomeningitis virus nucleoproteinAntigen delivery vectorLCMV epitopeSpecific CD8CTL activityHeterologous vaccinationVaccinationSalmonella typhimuriumPaucity of informationLethal dosesMiceImmunityCD8Oral deliveryImmunizationType III
1999
Salmonella typhimurium Encodes a Putative Iron Transport System within the Centisome 63 Pathogenicity Island
Zhou D, Hardt W, Galán J. Salmonella typhimurium Encodes a Putative Iron Transport System within the Centisome 63 Pathogenicity Island. Infection And Immunity 1999, 67: 1974-1981. PMID: 10085045, PMCID: PMC96555, DOI: 10.1128/iai.67.4.1974-1981.1999.Peer-Reviewed Original ResearchConceptsIron transport systemPathogenicity islandGrowth defectMinimal mediumPutative periplasmic binding proteinFur-dependent mannerPutative iron transport systemApparent growth defectS. typhimurium chromosomePeriplasmic binding proteinATP-binding proteinIron uptake systemOpen reading frameWild-type S. typhimuriumIron-restricted environmentNucleotide compositionPutative permeasesTransport systemExtensive homologyABC transportersReading frameSalmonella typhimuriumVirulence phenotypesUptake systemBinding proteinRole of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization
Zhou D, Mooseker M, Galán J. Role of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization. Science 1999, 283: 2092-2095. PMID: 10092234, DOI: 10.1126/science.283.5410.2092.Peer-Reviewed Original Research
1998
Delivery of Epitopes by the Salmonella Type III Secretion System for Vaccine Development
Rüssmann H, Shams H, Poblete F, Fu Y, Galán J, Donis R. Delivery of Epitopes by the Salmonella Type III Secretion System for Vaccine Development. Science 1998, 281: 565-568. PMID: 9677200, DOI: 10.1126/science.281.5376.565.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen PresentationAntigens, ViralBacterial ProteinsCytosolEndoplasmic ReticulumEpitopesHistocompatibility Antigens Class IHybridomasLymphocytic ChoriomeningitisLymphocytic choriomeningitis virusMiceMice, Inbred C57BLNucleoproteinsPeptide FragmentsProtein Tyrosine PhosphatasesRecombinant Fusion ProteinsSalmonella typhimuriumT-LymphocytesT-Lymphocytes, CytotoxicTumor Cells, CulturedVaccines, SyntheticViral Core ProteinsViral VaccinesConceptsImmune responseClass IS. typhimuriumAntigen delivery systemAntigen delivery vectorDelivery of epitopesSalmonella type III secretion systemLethal infectionViral epitopesVaccine developmentHost cell cytosolType III secretion systemInfectionSecretion systemIntracellular stagesAvirulent strainsEpitopesEfficient inducerSalmonella typhimuriumTyphimuriumDelivery systemResponseType III protein secretion systemIdentification of a Specific Chaperone for SptP, a Substrate of the Centisome 63 Type III Secretion System ofSalmonella typhimurium
Fu Y, Galán J. Identification of a Specific Chaperone for SptP, a Substrate of the Centisome 63 Type III Secretion System ofSalmonella typhimurium. Journal Of Bacteriology 1998, 180: 3393-3399. PMID: 9642193, PMCID: PMC107295, DOI: 10.1128/jb.180.13.3393-3399.1998.Peer-Reviewed Original ResearchMeSH KeywordsActinsAmino Acid SequenceBacterial ProteinsBase SequenceChromosomes, BacterialCloning, MolecularComplement Inactivator ProteinsCytoskeletonKineticsMolecular Sequence DataPlasmidsPolymerase Chain ReactionProtein Tyrosine PhosphatasesRecombinant Fusion ProteinsSalmonella typhimuriumSequence AlignmentSequence Homology, Amino AcidConceptsType III secretion systemSecretion systemSpecific chaperonesActin cytoskeletonTyrosine phosphataseType III protein secretion systemHost cell actin cytoskeletonHost cellsProtein secretion systemCell actin cytoskeletonHost cell responsesPulse-chase experimentsCytoplasmic chaperonesCentisome 63Effector proteinsTranscription factorsAcidic polypeptidesChaperonesResidues 15Function mutationsEffector moleculesCytoskeletonProteinSPTPSalmonella typhimurium
1997
The invasion‐associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cell
Collazo C, Galán J. The invasion‐associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cell. Molecular Microbiology 1997, 24: 747-756. PMID: 9194702, DOI: 10.1046/j.1365-2958.1997.3781740.x.Peer-Reviewed Original ResearchConceptsProtein secretion systemProtein translocationHost cellsSIP proteinHenle-407 cellsType III protein secretion systemType III secretion systemType III secretion apparatusType III systemSubset of targetsCentisome 63Secretion apparatusSecretion systemBacterial entryBacterial internalizationNull mutationSalmonella typhimuriumCytochalasin DTranslocation processTranslocationProteinMutationsPresence of gentamicinSipBS. typhimurium strain
1994
Contact with epithelial cells induces the formation of surface appendages on Salmonella typhimurium
Ginocchio C, Olmsted S, Wells C, Galán J. Contact with epithelial cells induces the formation of surface appendages on Salmonella typhimurium. Cell 1994, 76: 717-724. PMID: 8124710, DOI: 10.1016/0092-8674(94)90510-x.Peer-Reviewed Original ResearchConceptsCultured epithelial cellsEpithelial cellsFormation of appendagesCell-signaling eventsDe novo protein synthesisNovo protein synthesisMembrane rufflesSurface organellesBacterial internalizationBacteria Salmonella typhimuriumSalmonella typhimuriumInternalization eventsSurface appendagesHost cellsNonphagocytic cellsS. typhimuriumProtein synthesisBacterial uptakeSuch appendagesSalmonella mutantsInternalization processIntimate interactionCellsBacteriaTyphimurium
1992
Involvement of the epidermal growth factor receptor in the invasion of cultured mammalian cells by Salmonella typhimurium
Galán J, Pace J, Hayman M. Involvement of the epidermal growth factor receptor in the invasion of cultured mammalian cells by Salmonella typhimurium. Nature 1992, 357: 588-589. PMID: 1608468, DOI: 10.1038/357588a0.Peer-Reviewed Original ResearchConceptsHenle-407 cellsEpidermal growth factor receptorMammalian cellsGrowth factor receptorTyrosine phosphorylationCultured mammalian cellsProtein tyrosine phosphorylationFactor receptorInternalization of SalmonellaCell surface receptorsCultured epithelial cellsAddition of EGFSuch phosphorylationSignal transductionSalmonella genesSalmonella typhimuriumEGF receptorS. typhimuriumIsogenic strainsEscherichia coliPhosphorylationEpithelial cellsGenesInternalizationInvasion
1990
Cloning and characterization of the asd gene of Salmonella typhimurium: use in stable maintenance of recombinant plasmids in Salmonella vaccine strains
Galán J, Nakayama K, Curtiss R. Cloning and characterization of the asd gene of Salmonella typhimurium: use in stable maintenance of recombinant plasmids in Salmonella vaccine strains. Gene 1990, 94: 29-35. PMID: 2227450, DOI: 10.1016/0378-1119(90)90464-3.Peer-Reviewed Original Research
1989
Virulence and vaccine potential of phoP mutants of Salmonella typhimurium
Galán J, Curtiss R. Virulence and vaccine potential of phoP mutants of Salmonella typhimurium. Microbial Pathogenesis 1989, 6: 433-443. PMID: 2671582, DOI: 10.1016/0882-4010(89)90085-5.Peer-Reviewed Original ResearchConceptsDelayed-type hypersensitivity responseBALB/c miceProtective immune responseVirulent S. typhimuriumHypersensitivity responseC micePeyer's patchesImmune responseVaccine potentialImmunized animalsSalmonella antigensSR-11Lethal dosesPhoP mutantDosesS. typhimuriumVirulent parentSalmonella typhimuriumAvirulent mutantsLow levelsAnimalsDaysImmunizationTyphimuriumSpleen