2001
Maintenance of an unfolded polypeptide by a cognate chaperone in bacterial type III secretion
Stebbins C, Galán J. Maintenance of an unfolded polypeptide by a cognate chaperone in bacterial type III secretion. Nature 2001, 414: 77-81. PMID: 11689946, DOI: 10.1038/35102073.Peer-Reviewed Original ResearchConceptsEffector proteinsSecretion systemType III protein secretion systemBacterial type III secretionSpecific cytosolic chaperonesSuch effector proteinsType III secretion systemSecretion-competent stateVirulence effector proteinsProtein secretion systemChaperone-binding domainHost cell cytosolType III secretionType III apparatusCytosolic chaperonesUnfolded polypeptidesSecretion machineryCognate chaperoneÅ resolutionChaperonesCell cytosolChaperone moleculesEffective translocationBacterial pathogensHydrophobic interfaceStructural mimicry in bacterial virulence
Stebbins C, Galán J. Structural mimicry in bacterial virulence. Nature 2001, 412: 701-705. PMID: 11507631, DOI: 10.1038/35089000.Peer-Reviewed Original ResearchConceptsAmino acid sequence similarityConvergent evolutionCellular functionsSequence similarityDirect homologuesBacterial virulenceHost proteinsNew effectorsMicrobial pathogensFunctional mimicryStructural mimicryMolecular levelVirulence factorsMimicryStructural studiesHost factorsImportant mechanismHost activityHomologuesVirulenceEffectorsProteinPathogensSimilarityCommon and Contrasting Themes of Plant and Animal Diseases
Staskawicz B, Mudgett M, Dangl J, Galan J. Common and Contrasting Themes of Plant and Animal Diseases. Science 2001, 292: 2285-2289. PMID: 11423652, DOI: 10.1126/science.1062013.Peer-Reviewed Original ResearchConceptsHost-pathogen interactionsStudy of plantsDefense signalingHost defense responsesAnimal diseasesDefense responsesEffector proteinsAnimal pathogenesisBacterial pathogenesisPathogen virulencePathogen recognitionMolecular eventsHost resistancePlantsRecent studiesBacterial colonizationSignalingVirulenceProteinColonizationHostMechanismFuture studiesPathogenesisCharacterization of the mutS-proximal region of the Salmonella typhimurium SPI-1 identifies a group of pathogenicity island-associated genes
Pancetti A, Galán J. Characterization of the mutS-proximal region of the Salmonella typhimurium SPI-1 identifies a group of pathogenicity island-associated genes. FEMS Microbiology Letters 2001, 197: 203-208. PMID: 11313135, DOI: 10.1111/j.1574-6968.2001.tb10604.x.Peer-Reviewed Original ResearchConceptsPathogenicity islandSecretion systemType III protein secretion systemSPI-1Type III secretion systemHigher eukaryotic hostsProtein secretion systemSet of genesIron uptake systemExpression of genesSegment of DNAPotential pathogenicity islandsEukaryotic hostsFhlA geneGene blockCentisome 63Pig genesFunctional copyTranscription factorsSalmonella chromosomeUnknown functionUptake systemGenesBacterial pathogensHomologGenetic Analysis of Assembly of theSalmonella enterica Serovar Typhimurium Type III Secretion-Associated Needle Complex
Sukhan A, Kubori T, Wilson J, Galán J. Genetic Analysis of Assembly of theSalmonella enterica Serovar Typhimurium Type III Secretion-Associated Needle Complex. Journal Of Bacteriology 2001, 183: 1159-1167. PMID: 11157927, PMCID: PMC94988, DOI: 10.1128/jb.183.4.1159-1167.2001.Peer-Reviewed Original Research
2000
Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system
Kubori T, Sukhan A, Aizawa S, Galán J. Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 10225-10230. PMID: 10944190, PMCID: PMC27824, DOI: 10.1073/pnas.170128997.Peer-Reviewed Original ResearchConceptsType III secretion systemProtein secretion systemSecretion systemNeedle complexType III protein secretion systemHost cellular functionsSpecialized protein secretion systemType III secretion export apparatusType III secretionPathogenicity island 1Secretion complexPathogen's benefitExport apparatusCellular functionsAnimal cellsNeedle-like projectionsBacterial proteinsBacterium's abilityNeedle substructureIsland 1Host cellsMolecular characterizationNonphagocytic cellsNeedle componentBacterial pathogens
1999
Characterization of SprA, an AraC‐like transcriptional regulator encoded within the Salmonella typhimurium pathogenicity island 1
Eichelberg K, Hardt W, Galán J. Characterization of SprA, an AraC‐like transcriptional regulator encoded within the Salmonella typhimurium pathogenicity island 1. Molecular Microbiology 1999, 33: 139-152. PMID: 10411731, DOI: 10.1046/j.1365-2958.1999.01458.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAraC Transcription FactorBacterial ProteinsBiological TransportCells, CulturedChromosome MappingChromosomes, BacterialConsensus SequenceDNA-Binding ProteinsEpithelial CellsGene Expression Regulation, BacterialGenes, BacterialGenes, ReporterHelix-Loop-Helix MotifsMolecular Sequence DataMultigene FamilyPhylogenyRecombinant Fusion ProteinsRepressor ProteinsSalmonella typhimuriumSequence AlignmentSequence Homology, Amino AcidSignal TransductionTrans-ActivatorsTranscription FactorsTranscription, GeneticVirulenceConceptsType III secretion systemTranscriptional regulatory proteinsPathogenicity island 1Secretion systemRegulatory proteinsType III protein secretion systemSPI-1Island 1Host cellsProtein secretion systemTranscriptional regulatory cascadeSpecific transcriptional regulatory proteinsAdditional regulatory proteinsSignificant sequence similarityAraC-like transcriptional regulatorActin cytoskeletal rearrangementRegulation of expressionInvasion-associated genesExpression of genesNon-phagocytic cellsCentisome 63Effector proteinsTranscriptional regulatorsRegulatory cascadeSequence similarityType III Secretion Machines: Bacterial Devices for Protein Delivery into Host Cells
Galán J, Collmer A. Type III Secretion Machines: Bacterial Devices for Protein Delivery into Host Cells. Science 1999, 284: 1322-1328. PMID: 10334981, DOI: 10.1126/science.284.5418.1322.Peer-Reviewed Original ResearchConceptsSecretion systemComplex protein secretion systemsHost cellsType III secretion systemBacterial effector proteinsProtein secretion systemAnimal pathogenic bacteriaHost cellular functionsGram-negative pathogenic bacteriaBacterial devicesPathogenic bacteriaEffector proteinsCellular functionsFlagellar apparatusBacterial speciesEffector moleculesProtein deliveryBacteriaCellsNovel preventionBiologySpeciesPlantsProteinTherapeutic approachesInteraction of Salmonella with host cells through the centisome 63 type III secretion system
Galán J. Interaction of Salmonella with host cells through the centisome 63 type III secretion system. Current Opinion In Microbiology 1999, 2: 46-50. PMID: 10047557, DOI: 10.1016/s1369-5274(99)80008-3.Peer-Reviewed Original Research
1998
Interactions of Salmonella with host cells: Encounters of the closest kind
Galán J. Interactions of Salmonella with host cells: Encounters of the closest kind. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 14006-14008. PMID: 9826642, PMCID: PMC33922, DOI: 10.1073/pnas.95.24.14006.Peer-Reviewed Original ResearchA substrate of the centisome 63 type III protein secretion system of Salmonella typhimurium is encoded by a cryptic bacteriophage
Hardt W, Urlaub H, Galán J. A substrate of the centisome 63 type III protein secretion system of Salmonella typhimurium is encoded by a cryptic bacteriophage. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 2574-2579. PMID: 9482928, PMCID: PMC19418, DOI: 10.1073/pnas.95.5.2574.Peer-Reviewed Original ResearchConceptsType III protein secretion systemType III secretion systemProtein secretion systemSecretion systemEffector proteinsInvasion-associated type III secretion systemCluster of genesTail fiber proteinHost cell cytoplasmSouthern hybridization analysisMobile genetic elementsCultured epithelial cellsInduction of apoptosisSopE proteinEnterica serovar TyphimuriumBacteriophage genesBacterial entryCytoskeletal rearrangementsS. enterica serovar TyphimuriumGenetic elementsPathogenicity islandFiber proteinHybridization analysisCell cytoplasmEffector molecules‘Avirulence genes’ in animal pathogens?
Galán J. ‘Avirulence genes’ in animal pathogens? Trends In Microbiology 1998, 6: 3-6. PMID: 9481815, DOI: 10.1016/s0966-842x(97)01183-9.Peer-Reviewed Original Research
1996
A secreted protein tyrosine phosphatase with modular effector domains in the bacterial pathogen Salmonella typhimurlum
Kaniga K, Uralil J, Bliska J, Galán J. A secreted protein tyrosine phosphatase with modular effector domains in the bacterial pathogen Salmonella typhimurlum. Molecular Microbiology 1996, 21: 633-641. PMID: 8866485, DOI: 10.1111/j.1365-2958.1996.tb02571.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBacterial ProteinsBase SequenceCell LineChromosomes, BacterialDisease Models, AnimalDNA, BacterialEpithelial CellsFemaleHumansMacrophagesMiceMice, Inbred BALB CMolecular Sequence DataProtein Tyrosine PhosphatasesSalmonella InfectionsSalmonella typhimuriumSequence Homology, Amino AcidVirulenceConceptsProtein tyrosine phosphataseTyrosine phosphataseEffector proteinsCatalytic domainHost cell signal transduction pathwaysBacterial pathogen Salmonella typhimuriumSignal transduction pathwaysCritical Cys residuesAmino-terminal regionCarboxy-terminal regionPathogen Salmonella typhimuriumCell regulatory moleculesSequence similarityEffector domainSignaling functionsExport proteinCys residuesRegulatory moleculesExoenzyme SSequence analysisPeptide substratesPhosphatase activityCatalytic mechanismProteinYersinia spp
1994
The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteins
Kaniga K, Bossio J, Galán J. The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteins. Molecular Microbiology 1994, 13: 555-568. PMID: 7997169, DOI: 10.1111/j.1365-2958.1994.tb00450.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAraC Transcription FactorBacterial Outer Membrane ProteinsBacterial ProteinsBase SequenceCells, CulturedCloning, MolecularDNA-Binding ProteinsGene Expression Regulation, BacterialGenes, BacterialMembrane ProteinsMembrane Transport ProteinsMolecular Sequence DataMutagenesisRecombinant ProteinsRepressor ProteinsRestriction MappingSalmonella typhimuriumSequence Homology, Amino AcidTranscription FactorsTranscription, GeneticVirulenceConceptsCultured epithelial cellsSalmonella typhimurium genesNon-polar mutationT7 RNA polymerase-based expression systemEpithelial cellsAraC familyTranscription regulatorsInv locusTyphimurium genesNucleotide sequenceSalmonella entryInvGExpression systemInvFGenesProteinSequenceMutationsS. typhimuriumFamilyTranslocasesSimilar sizeCellsMembersHomologues
1993
Cloning and molecular characterization of a gene involved in Salmonella adherence and invasion of cultured epithelial cells
Altmeyer R, McNern J, Bossio JC, Rosenshine I, Finlay B, Galán J. Cloning and molecular characterization of a gene involved in Salmonella adherence and invasion of cultured epithelial cells. Molecular Microbiology 1993, 7: 89-98. PMID: 8382333, DOI: 10.1111/j.1365-2958.1993.tb01100.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBacterial AdhesionBacterial ProteinsBase SequenceCells, CulturedDNA Transposable ElementsEpithelial CellsEpitheliumGenes, BacterialMolecular Sequence DataMutagenesisPhenotypeRecombinant Fusion ProteinsSalmonellaSalmonella typhimuriumSequence Homology, Nucleic AcidVirulenceConceptsCultured epithelial cellsInvH geneT7 RNA polymerase expression systemBacterial membrane fractionsEpithelial cellsSignal sequenceExtensive homologyColony hybridization analysisNucleotide sequenceGene productsHomologous sequencesExpression systemException of strainsMolecular characterizationGenesHybridization analysisMembrane fractionCultured cellsEscherichia coliPolypeptideInsertion sequence IS3E. coliOrganismsInvHSequence
1989
Virulence and vaccine potential of phoP mutants of Salmonella typhimurium
Galán J, Curtiss R. Virulence and vaccine potential of phoP mutants of Salmonella typhimurium. Microbial Pathogenesis 1989, 6: 433-443. PMID: 2671582, DOI: 10.1016/0882-4010(89)90085-5.Peer-Reviewed Original ResearchConceptsDelayed-type hypersensitivity responseBALB/c miceProtective immune responseVirulent S. typhimuriumHypersensitivity responseC micePeyer's patchesImmune responseVaccine potentialImmunized animalsSalmonella antigensSR-11Lethal dosesPhoP mutantDosesS. typhimuriumVirulent parentSalmonella typhimuriumAvirulent mutantsLow levelsAnimalsDaysImmunizationTyphimuriumSpleen