2003
A Salmonella protein causes macrophage cell death by inducing autophagy
Hernandez LD, Pypaert M, Flavell RA, Galán J. A Salmonella protein causes macrophage cell death by inducing autophagy. Journal Of Cell Biology 2003, 163: 1123-1131. PMID: 14662750, PMCID: PMC2173598, DOI: 10.1083/jcb.200309161.Peer-Reviewed Original ResearchConceptsMembrane fusion activityCell deathType III protein secretion systemFusion activityProtein secretion systemMacrophage cell deathEndoplasmic reticulum markerSalmonella-infected macrophagesSecretion systemSalmonella proteinsAutophagic vesiclesMutant formsSipBHost cellsCultured cellsMitochondriaMembrane structureSalmonella entericaProteinCausative agentMarked accumulationMacrophagesCellsAutophagosomesAutophagy
2000
Alternative Strategies for Becoming an Insider Lessons from the Bacterial World
Galán J. Alternative Strategies for Becoming an Insider Lessons from the Bacterial World. Cell 2000, 103: 363-366. PMID: 11081622, DOI: 10.1016/s0092-8674(00)00127-6.Peer-Reviewed Original ResearchComplex Function for SicA, a Salmonella enterica Serovar Typhimurium Type III Secretion-Associated Chaperone
Tucker S, Galán J. Complex Function for SicA, a Salmonella enterica Serovar Typhimurium Type III Secretion-Associated Chaperone. Journal Of Bacteriology 2000, 182: 2262-2268. PMID: 10735870, PMCID: PMC111276, DOI: 10.1128/jb.182.8.2262-2268.2000.Peer-Reviewed Original Research
1998
Supramolecular Structure of the Salmonella typhimurium Type III Protein Secretion System
Kubori T, Matsushima Y, Nakamura D, Uralil J, Lara-Tejero M, Sukhan A, Galán J, Aizawa S. Supramolecular Structure of the Salmonella typhimurium Type III Protein Secretion System. Science 1998, 280: 602-605. PMID: 9554854, DOI: 10.1126/science.280.5363.602.Peer-Reviewed Original ResearchThe Salmonella typhimurium tyrosine phosphatase SptP is translocated into host cells and disrupts the actin cytoskeleton
Fu Y, Galán J. The Salmonella typhimurium tyrosine phosphatase SptP is translocated into host cells and disrupts the actin cytoskeleton. Molecular Microbiology 1998, 27: 359-368. PMID: 9484891, DOI: 10.1046/j.1365-2958.1998.00684.x.Peer-Reviewed Original ResearchConceptsActin cytoskeletonHost cell actin cytoskeletonFusion proteinHost cellsAmino acidsAmino-terminal halfCell actin cytoskeletonCultured cells resultsWild-type toxinCultured epithelial cellsEffector proteinsModular domainsStress fibersActive GSTCytoskeletonProteinCells resultsEpithelial cellsGSTSPTPProlonged incubationTranslocationCellsS. typhimuriumMicroinjection
1997
The invasion‐associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cell
Collazo C, Galán J. The invasion‐associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cell. Molecular Microbiology 1997, 24: 747-756. PMID: 9194702, DOI: 10.1046/j.1365-2958.1997.3781740.x.Peer-Reviewed Original ResearchConceptsProtein secretion systemProtein translocationHost cellsSIP proteinHenle-407 cellsType III protein secretion systemType III secretion systemType III secretion apparatusType III systemSubset of targetsCentisome 63Secretion apparatusSecretion systemBacterial entryBacterial internalizationNull mutationSalmonella typhimuriumCytochalasin DTranslocation processTranslocationProteinMutationsPresence of gentamicinSipBS. typhimurium strain
1996
Salmonella spp. are cytotoxic for cultured macrophages
Chen L, Kaniga K, Galán J. Salmonella spp. are cytotoxic for cultured macrophages. Molecular Microbiology 1996, 21: 1101-1115. PMID: 8885278, DOI: 10.1046/j.1365-2958.1996.471410.x.Peer-Reviewed Original ResearchConceptsProtein secretion systemNon-phagocytic cellsBacterial internalizationType III protein secretion systemCell deathFragmentation of chromatinWild-type Salmonella typhimuriumMacrophage cell deathBone marrow-derived murine macrophagesMurine macrophagesFeatures of apoptosisCentisome 63Membrane rufflingSecretion apparatusMembrane blebbingBacterial entrySalmonella chromosomeMacrophage cytotoxicityHost rangeCytoplasmic nucleosomesApoptotic bodiesBacterial uptakeCytochalasin DBiochemical techniquesMutations
1994
The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteins
Kaniga K, Bossio J, Galán J. The Salmonella typhimurium invasion genes invF and invG encode homologues of the AraC and PulD family of proteins. Molecular Microbiology 1994, 13: 555-568. PMID: 7997169, DOI: 10.1111/j.1365-2958.1994.tb00450.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAraC Transcription FactorBacterial Outer Membrane ProteinsBacterial ProteinsBase SequenceCells, CulturedCloning, MolecularDNA-Binding ProteinsGene Expression Regulation, BacterialGenes, BacterialMembrane ProteinsMembrane Transport ProteinsMolecular Sequence DataMutagenesisRecombinant ProteinsRepressor ProteinsRestriction MappingSalmonella typhimuriumSequence Homology, Amino AcidTranscription FactorsTranscription, GeneticVirulenceConceptsCultured epithelial cellsSalmonella typhimurium genesNon-polar mutationT7 RNA polymerase-based expression systemEpithelial cellsAraC familyTranscription regulatorsInv locusTyphimurium genesNucleotide sequenceSalmonella entryInvGExpression systemInvFGenesProteinSequenceMutationsS. typhimuriumFamilyTranslocasesSimilar sizeCellsMembersHomologues