2021
Colorectal Cancer Stem Cell States Uncovered by Simultaneous Single‐Cell Analysis of Transcriptome and Telomeres
Wang H, Gong P, Chen T, Gao S, Wu Z, Wang X, Li J, Marjani SL, Costa J, Weissman SM, Qi F, Pan X, Liu L. Colorectal Cancer Stem Cell States Uncovered by Simultaneous Single‐Cell Analysis of Transcriptome and Telomeres. Advanced Science 2021, 8: 2004320. PMID: 33898197, PMCID: PMC8061397, DOI: 10.1002/advs.202004320.Peer-Reviewed Original ResearchConceptsCancer stem cellsCancer epithelial cellsTumor-initiating cellsCancer stem cell stateEpithelial cellsStem cell stateStem cellsRare cancer stem cellsSimultaneous single-cell analysisHeterogeneity of CSCsSingle-cell analysisNumber variation patternsHippo/YAPDepth transcriptomeSmart-seq2Phylogenetic relationshipsNormal stem cellsCell statesNonproliferative natureColorectal cancerDisplay plasticityDormant stateShort telomeresTelomerase activityTranscriptome
2020
Synovial Myxoma: Found in Canines, Rabbits, and Now Humans Too?: A Case Report.
Munger AM, Ibe IK, Rottmann D, Conway D, Costa J, Lindskog DM. Synovial Myxoma: Found in Canines, Rabbits, and Now Humans Too?: A Case Report. JBJS Case Connector 2020, 10: e0490. PMID: 32224675, DOI: 10.2106/jbjs.cc.19.00490.Peer-Reviewed Case Reports and Technical NotesConceptsSynovial myxomaCase reportRight knee painYear old womanCluster of differentiationLow proliferative activityAggressive massKnee painPathologic reviewIntralesional resectionArthroscopic synovectomyKnee aspirationSynovial cellsInfiltrative tumorsMyxoid stromaProliferative activityPainSynovectomyMyxomaSlow growingReportResectionPatientsEffusionHumans
2019
Biliary reflux as a causal factor in hypopharyngeal carcinoma: New clinical evidence and implications
Sasaki CT, Doukas SG, Costa J, Vageli DP. Biliary reflux as a causal factor in hypopharyngeal carcinoma: New clinical evidence and implications. Cancer 2019, 125: 3554-3565. PMID: 31310330, DOI: 10.1002/cncr.32369.Peer-Reviewed Original ResearchConceptsHuman hypopharyngeal squamous cell carcinomaAdjacent normal tissuesIL-6Reflux diseaseHypopharyngeal squamous cell carcinomaMiR-375Respective adjacent normal tissuesMiR-21Pilot studyEsophageal reflux diseaseIndependent risk factorNew clinical evidenceSquamous cell carcinomaOncomiR miR-21NF-κB activationMiR-21/miRTumor suppressor miR-375Quantitative polymerase chain reactionMessenger RNABiliary refluxHypopharyngeal carcinogenesisControl patientsLaryngopharyngeal refluxClinical evidenceHypopharyngeal carcinoma
2018
A brave new world
Costa J. A brave new world. Virchows Archiv 2018, 473: 141-144. PMID: 29569207, DOI: 10.1007/s00428-018-2338-9.Peer-Reviewed Original Research
2017
A 49-Year-Old Man with Subacute Respiratory Failure and Interstitial Lung Opacities
Rudkovskaia AA, Lo YC, Brady V, Costa J, Fares WH. A 49-Year-Old Man with Subacute Respiratory Failure and Interstitial Lung Opacities. American Journal Of Case Reports 2017, 18: 941-944. PMID: 28855497, PMCID: PMC5590515, DOI: 10.12659/ajcr.903742.Peer-Reviewed Original ResearchConceptsPulmonary tumor thrombotic microangiopathyRight heart catheterizationPulmonary arterial hypertensionTrans-bronchial biopsyPulmonary hypertensionDiagnosis of PTTMPulseless electrical activity cardiac arrestBackground Pulmonary arterial hypertensionGastro-esophageal junction adenocarcinomaNew bilateral pulmonary infiltratesInitiation of trastuzumabMicroscopic tumor embolismSubacute respiratory failureTrans-thoracic echocardiogramTumor thrombotic microangiopathyBilateral pulmonary infiltratesSevere pulmonary hypertensionComplaints of coughRV systolic functionShortness of breathComputed tomography angiographySmall pulmonary vesselsSmooth muscle proliferationPulmonary infiltratesArterial hypertension
2014
Genetic alterations in chondrosarcomas – keys to targeted therapies?
Samuel AM, Costa J, Lindskog DM. Genetic alterations in chondrosarcomas – keys to targeted therapies? Cellular Oncology 2014, 37: 95-105. PMID: 24458248, DOI: 10.1007/s13402-014-0166-8.Peer-Reviewed Original ResearchConceptsBenign cartilaginous lesionsCartilaginous lesionsMalignant transformationIhh/PTHrPTwo-hit scenarioPrimary bone tumorsGenetic alterationsDruggable molecular targetsKey genetic alterationsHuman tumor typesResultsCurrent dataBone tumorsMalignant tumorsSarcoma typesMalignant chondrosarcomasKey molecular changesNormal cell cycle controlTumor typesMalignant cellsChondrosarcoma developmentLesionsMolecular targetsTumorsEarly-stage eventsDifferentiation of chondrocytes
2010
Personalized approach to prostate cancer prognosis.
Donovan M, Costa J, Cordon-Cardo C. Personalized approach to prostate cancer prognosis. Minerva Urologica E Nefrologica 2010, 62: 231-9. PMID: 20940693.Peer-Reviewed Original ResearchConceptsProstate-specific antigenProstate needle biopsyNeedle biopsyProstate cancerProstatectomy specimenDiagnostic prostate needle biopsiesSerum prostate-specific antigenPatient-specific risk profileClinical-pathologic variablesExtent of diseaseManagement of patientsAncillary diagnostic studiesTreatment decision processPrognostic featuresTumor differentiationBreast cancerIndividual patientsCancer prognosisSpecific antigenClinical judgmentHealth outcomesMost urologistsTumor typesClinical decisionPatient attributes
2008
NIH consensus development statement on management of hepatitis B.
Belongia E, Costa J, Gareen I, Grem J, Inadomi J, Kern E, McHugh J, Petersen G, Rein M, Sorrell M, Strader D, Trotter H. NIH consensus development statement on management of hepatitis B. NIH Consensus And State-of-the-Science Statements 2008, 25: 1-29. PMID: 18949020.Peer-Reviewed Original ResearchConceptsAnti-HBV therapyHBV DNAHepatocellular carcinomaChronic HBVALT levelsHepatitis BHepatitis B.Advanced fibrosisLiver failureHBV genotype C infectionRepresentative prospective cohort studyElevated HBV DNAHBV prevalence rateImmune active phaseImmune-tolerant phaseLatent HBV infectionLiver-specific mortalityLow replicative phaseChronic hepatitis BPlacebo-controlled trialProspective cohort studyGenotype C infectionAcute liver failureClinical health outcomesBurden of disease
2002
Semireannealing, Single-Stranded Conformational Polymorphism: A Novel and Effective Tool for the Diagnosis of T-Cell Clonality
Crisi G, Emanuel J, Johnson C, Crotty P, Costa J, Tallini G. Semireannealing, Single-Stranded Conformational Polymorphism: A Novel and Effective Tool for the Diagnosis of T-Cell Clonality. Applied Immunohistochemistry & Molecular Morphology 2002, 11: 67-74. PMID: 12045709, DOI: 10.1097/00019606-200206000-00002.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceClone CellsDNA, NeoplasmElectrophoresis, CapillaryElectrophoresis, Polyacrylamide GelGene RearrangementHumansLymph NodesLymphoma, B-CellLymphoma, T-CellMolecular Sequence DataNucleic Acid ConformationPolymerase Chain ReactionPolymorphism, Single-Stranded ConformationalPseudolymphomaReceptors, Antigen, T-Cell, gamma-deltaSequence AlignmentSequence Analysis, DNAp53 Mutations as Tumor Markers in Fine Needle Aspirates of Palpable Breast Masses
Dillon DA, Hipolito E, Zheng K, Rimm DL, Costa JC. p53 Mutations as Tumor Markers in Fine Needle Aspirates of Palpable Breast Masses. Acta Cytologica 2002, 46: 841-847. PMID: 12365217, DOI: 10.1159/000327057.Peer-Reviewed Original ResearchMeSH KeywordsAbscessAdenocarcinomaAdultAgedAged, 80 and overAmino Acid SubstitutionBiomarkers, TumorBiopsy, NeedleBreast NeoplasmsCarcinoma, Ductal, BreastCodon, TerminatorCystsDNA Mutational AnalysisDNA, NeoplasmExonsFemaleFrameshift MutationGenes, p53GenotypeHumansMiddle AgedMutationPolymorphism, Single-Stranded ConformationalRetrospective StudiesConceptsFine needle aspiratesP53 exons 5Breast massesPolymerase chain reactionNeedle aspiratesP53 mutationsSingle-strand conformational polymorphism analysisSubsequent excisional biopsyPalpable breast massesPotential diagnostic utilityDefinitive cytologic diagnosisTumor cell markersExon 5Molecular diagnostic markersExcisional biopsyBenign cytologyBreast carcinomaSuspicious cytologyRetrospective analysisCytologic diagnosisTumor markersDiagnostic criteriaBiopsy tissueMorphologic diagnosisDiagnostic utility
2001
Multiplex Detection of Hotspot Mutations by Rolling Circle-Enabled Universal Microarrays
Ladner D, Leamon J, Hamann S, Tarafa G, Strugnell T, Dillon D, Lizardi P, Costa J. Multiplex Detection of Hotspot Mutations by Rolling Circle-Enabled Universal Microarrays. Laboratory Investigation 2001, 81: 1079-1086. PMID: 11502859, DOI: 10.1038/labinvest.3780320.Peer-Reviewed Original ResearchRapid, Efficient Genotyping of Clinical Tumor Samples by Laser-Capture Microdissection/PCR/SSCP
Dillon D, Zheng K, Costa J. Rapid, Efficient Genotyping of Clinical Tumor Samples by Laser-Capture Microdissection/PCR/SSCP. Experimental And Molecular Pathology 2001, 70: 195-200. PMID: 11417998, DOI: 10.1006/exmp.2001.2362.Peer-Reviewed Original ResearchConceptsLaser capture microdissectionP53 exons 5PCR/SSCPTumor genotypingPure tumor cell populationsSSCP/sequencingSurgical pathology reportsRoutine clinical useClinical tumor samplesExon 5Colorectal adenocarcinomaTherapeutic responsePathology reportsTumor cell populationLaser-microdissected samplesClinical practiceTumor samplesClinical useSporadic mutationsExon 1Molecular analysisCell populationsDirect sequencingMutation analysisPCR productsDetection of the Mutated K-Ras Biomarker in Colorectal Carcinoma
Doolittle B, Emanuel J, Tuttle C, Costa J. Detection of the Mutated K-Ras Biomarker in Colorectal Carcinoma. Experimental And Molecular Pathology 2001, 70: 289-301. PMID: 11418008, DOI: 10.1006/exmp.2001.2364.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkers, TumorColorectal NeoplasmsDeoxyribonuclease EcoRIDNADNA, NeoplasmFecesGenes, rasHumansIndicators and ReagentsMutationPolymerase Chain ReactionPolymorphism, Single-Stranded ConformationalQuaternary Ammonium CompoundsReference ValuesReproducibility of ResultsSite-Specific DNA-Methyltransferase (Adenine-Specific)Trimethyl Ammonium CompoundsConceptsColorectal carcinomaStool samplesHealthy patientsK-rasReliable screening testDouble-blind formatK-ras mutationsWild-type K-rasK-ras oncogeneK-ras sequencesCommon malignancyCRC tumorsPolymerase chain reactionEarly lesionsStool DNANoninvasive screenScreening testTumor tissueGenetic biomarkersHuman stoolPatientsEarly detectionHigh false positive rateSingle-strand conformation polymorphismTissue DNA
2000
The utility of Ki-ras mutation analysis in the cytologic diagnosis of pancreatobiliary neoplasma.
Dillon DA, Johnson CC, Topazian MD, Tallini G, Rimm DL, Costa JC. The utility of Ki-ras mutation analysis in the cytologic diagnosis of pancreatobiliary neoplasma. The Cancer Journal 2000, 6: 294-301. PMID: 11079168.Peer-Reviewed Original ResearchConceptsFine needle aspiratesBile duct brushingsCytologic diagnosisPositive predictive valueCommon bile duct brushingsDuct brushingsPancreatobiliary carcinomaPredictive valueMutation patternsBiliary tract carcinomaPrevious retrospective studyAvailable clinical informationConsecutive clinical specimensDefinitive cytologic diagnosisPolymerase chain reaction/single-strand conformation polymorphism analysisRoutine cytologic diagnosisPositive cytologyRetrospective studySuspicious morphologyCytologic evaluationSuspicious cytologyPancreatobiliary tractClinical informationMorphologic diagnosisNeoplastic cells
1999
Analysis of K-ras, N-ras, H-ras, and p53 in Lung Neuroendocrine Neoplasms
Couce M, Bautista D, Costa J, Carter D. Analysis of K-ras, N-ras, H-ras, and p53 in Lung Neuroendocrine Neoplasms. Applied Immunohistochemistry & Molecular Morphology 1999, 8: 71-79. PMID: 10475381, DOI: 10.1097/00019606-199906000-00003.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAllelesDNA Mutational AnalysisExonsFemaleGenes, rasHumansImmunohistochemistryLung NeoplasmsMaleMiddle AgedMutation, MissenseNeuroendocrine TumorsPoint MutationPolymerase Chain ReactionPolymorphism, GeneticPolymorphism, Single-Stranded ConformationalRetrospective StudiesTumor Suppressor Protein p53ConceptsLarge cell neuroendocrine carcinomaPulmonary neuroendocrine tumorsAtypical carcinoidNeuroendocrine tumorsTypical carcinoidP53 geneK-rasCell neuroendocrine carcinomaSingle-strand conformation polymorphismHigh-grade tumorsHigh-grade carcinomaH-ras mutationsLung neuroendocrine neoplasmsN-rasK-ras mutationsH-RasExon 5Neuroendocrine carcinomaPolymerase chain reactionNeuroendocrine neoplasmsPositive immunostainingGenotypic mutationsN-ras geneTumorsBeta- and gamma-catenin mutations, but not E-cadherin inactivation, underlie T-cell factor/lymphoid enhancer factor transcriptional deregulation in gastric and pancreatic cancer.
Caca K, Kolligs FT, Ji X, Hayes M, Qian J, Yahanda A, Rimm DL, Costa J, Fearon ER. Beta- and gamma-catenin mutations, but not E-cadherin inactivation, underlie T-cell factor/lymphoid enhancer factor transcriptional deregulation in gastric and pancreatic cancer. Molecular Cancer Research 1999, 10: 369-76. PMID: 10392898.Peer-Reviewed Original ResearchMeSH KeywordsAdenomatous Polyposis Coli ProteinAmino Acid SequenceAnimalsBeta CateninCadherinsCytoskeletal ProteinsDesmoplakinsDNA-Binding ProteinsGamma CateninGene Expression Regulation, NeoplasticHMGB ProteinsHumansLymphoid Enhancer-Binding Factor 1Molecular Sequence DataMutagenesisPancreatic NeoplasmsStomach NeoplasmsTCF Transcription FactorsTrans-ActivatorsTranscription Factor 7-Like 1 ProteinTranscription FactorsTranscription, GeneticTumor Cells, CulturedConceptsPhosphorylation sitesMutant proteinsGlycogen synthase kinase 3beta phosphorylation sitesGlycogen synthase kinase-3betaFactor transcription factorsPotential phosphorylation sitesSynthase kinase-3betaTCF transcriptional activityE-cadherin inactivationNH2-terminal deletionsRole of APCImportant binding partnerSerine 28TCF transcriptionTranscriptional deregulationT-cell factorBinding partnerTranscription factorsAPC proteinKinase-3betaTranscriptional activityNH2 terminusAdenomatous polyposis coli (APC) mutationsCell adhesionPancreatic cancer lines
1998
The Molecular Biology of Breast Cancer: Accelerating Clinical Applications
Dillon D, Howe C, Bosari S, Costa J. The Molecular Biology of Breast Cancer: Accelerating Clinical Applications. Critical Reviews™ In Oncogenesis 1998, 9: 125-140. PMID: 9973246, DOI: 10.1615/critrevoncog.v9.i2.30.Peer-Reviewed Original ResearchConceptsBreast cancerClinical applicationPreclinical disease modelsRegional oncology networkMolecular eventsOncology NetworkPractice guidelinesBRCA-1BRCA-2CancerGrowth factorTranslational researchDisease modelsPathogenesisCell deathBasic scienceCell cycle controlMetastasisClinicTherapyCycle controlMolecular biologyAbnormalitiesDiagnosis
1996
Highly Sensitive Nonradioactive Single-strand Conformational Polymorphism
Emanuel J, Damico C, Ahn S, Bautista D, Costa J. Highly Sensitive Nonradioactive Single-strand Conformational Polymorphism. Applied Immunohistochemistry & Molecular Morphology 1996, 5: 260-264. PMID: 8955617, DOI: 10.1097/00019606-199612000-00006.Peer-Reviewed Original ResearchIdentification of mutations in the Ki-ras gene in human retinoblastoma.
Bautista D, Emanuel J, Granville C, Howard R, Costa J. Identification of mutations in the Ki-ras gene in human retinoblastoma. Investigative Ophthalmology & Visual Science 1996, 37: 2313-7. PMID: 8843915.Peer-Reviewed Original ResearchConceptsKi-ras geneKi-rasIdentification of mutationsSelective growth advantageGenomic sequencesSingle-strand conformation polymorphismGrowth advantageTumor cell populationGenesExon 1Ki-ras mutationsMutationsExon 2Cell populationsConformation polymorphismHuman retinoblastomaClonal mutationsDirect sequencingPolymerase chain reactionTumor samplesLight microscopyMutational statusChain reactionRetinoblastomaOncogeneDetection of rare circulating human colon tumor cells in a nude mouse xenograft model
Pages J, Sordat B, Bautista D, Costa J, Benhattar J. Detection of rare circulating human colon tumor cells in a nude mouse xenograft model. Cancer Letters 1996, 106: 139-144. PMID: 8827058, DOI: 10.1016/0304-3835(96)04319-4.Peer-Reviewed Original ResearchConceptsHuman colon tumor cellsTumor cellsColon tumor cellsSensitive allele-specific polymerase chain reactionExperimental modelNude mouse xenograft modelK-ras mutationsMouse xenograft modelAllele-specific polymerase chain reactionK-ras geneLymph nodesPrimary implantationSpecific polymerase chain reactionHuman colon carcinoma cellsMicrometastatic depositsPolymerase chain reactionColon carcinoma cellsXenograft modelNude miceGAT mutationMetastatic processCarcinoma cellsBloodChain reactionOccurrence of cells