2022
High-affinity, neutralizing antibodies to SARS-CoV-2 can be made without T follicular helper cells
Chen JS, Chow RD, Song E, Mao T, Israelow B, Kamath K, Bozekowski J, Haynes WA, Filler RB, Menasche BL, Wei J, Alfajaro MM, Song W, Peng L, Carter L, Weinstein JS, Gowthaman U, Chen S, Craft J, Shon JC, Iwasaki A, Wilen CB, Eisenbarth SC. High-affinity, neutralizing antibodies to SARS-CoV-2 can be made without T follicular helper cells. Science Immunology 2022, 7: eabl5652. PMID: 34914544, PMCID: PMC8977051, DOI: 10.1126/sciimmunol.abl5652.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionSARS-CoV-2Follicular helper cellsB cell responsesHelper cellsAntibody productionCell responsesSARS-CoV-2 vaccinationB-cell receptor sequencingSevere COVID-19Cell receptor sequencingIndependent antibodiesT cell-B cell interactionsViral inflammationAntiviral antibodiesImmunoglobulin class switchingVirus infectionGerminal centersViral infectionClonal repertoireInfectionAntibodiesClass switchingCOVID-19Patients
2021
T Follicular Regulatory Cells: Choreographers of Productive Germinal Center Responses
Lu Y, Craft J. T Follicular Regulatory Cells: Choreographers of Productive Germinal Center Responses. Frontiers In Immunology 2021, 12: 679909. PMID: 34177925, PMCID: PMC8222975, DOI: 10.3389/fimmu.2021.679909.Peer-Reviewed Original ResearchConceptsTfr cellsTreg cellsHumoral immunityGerminal centersAntigen-specific B cell responsesFollicular helper cellsFollicular regulatory (Tfr) cellsB cell folliclesRegulatory T cellsB cell autoreactivityB cell responsesGerminal center responseB cell toleranceVaccine design strategiesTranscription factor Bcl6Regulatory cellsHelper cellsImmune homeostasisProductive immunityT cellsViral infectionCenter responseCell responsesGC responseImmune challengeNeoantigen driven B cell and CD4+ T follicular helper cell collaboration promotes robust anti-tumor CD8+ T cell responses
Cui C, Joshi N, Craft J. Neoantigen driven B cell and CD4+ T follicular helper cell collaboration promotes robust anti-tumor CD8+ T cell responses. The Journal Of Immunology 2021, 206: 57.01-57.01. DOI: 10.4049/jimmunol.206.supp.57.01.Peer-Reviewed Original ResearchT cell responsesAnti-tumor CD8Tfh cellsB cellsCell responsesIL-21Cell collaborationProtective anti-tumor responsesTfh-B cell interactionsTumor-specific B cellsGC B cell responsesTumor-specific CD4Anti-tumor immunityFavorable clinical outcomeAnti-tumor responseIL-21 receptorB cell responsesLung adenocarcinoma patientsAnalysis of survivalT cell-B cell interactionsGerminal center formationCell interactionsGC B cellsAbstract CD4Effector CD8Type I Interferon–Activated STAT4 Regulation of Follicular Helper T Cell–Dependent Cytokine and Immunoglobulin Production in Lupus
Dong X, Antao OQ, Song W, Sanchez GM, Zembrzuski K, Koumpouras F, Lemenze A, Craft J, Weinstein JS. Type I Interferon–Activated STAT4 Regulation of Follicular Helper T Cell–Dependent Cytokine and Immunoglobulin Production in Lupus. Arthritis & Rheumatology 2021, 73: 478-489. PMID: 33512094, PMCID: PMC7914134, DOI: 10.1002/art.41532.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAntibody FormationAutoantibodiesB-LymphocytesCase-Control StudiesCytokinesDisease Models, AnimalFemaleHumansImmunoglobulinsInterferon Type IInterferon-gammaInterleukinsLupus Erythematosus, SystemicMaleMice, Inbred MRL lprMiddle AgedRNA-SeqSTAT4 Transcription FactorT Follicular Helper CellsConceptsSystemic lupus erythematosusTfh-like cellsTfh cellsIL-21Human lupusDisease activityCytokine productionSTAT4 activationImmunoglobulin productionPathogenic B cell responsesCourse of lupusClinical disease activityT cell secretionLupus-prone miceHealthy control subjectsCourse of diseaseB cell responsesCytokine interleukin-21Potential therapeutic targetType I IFNB cell maturationSLE patientsPathogenic cytokinesLupus erythematosusInterleukin-21
2020
CD4+ follicular regulatory T cells optimize the influenza virus–specific B cell response
Lu Y, Jiang R, Freyn AW, Wang J, Strohmeier S, Lederer K, Locci M, Zhao H, Angeletti D, O’Connor K, Kleinstein SH, Nachbagauer R, Craft J. CD4+ follicular regulatory T cells optimize the influenza virus–specific B cell response. Journal Of Experimental Medicine 2020, 218: e20200547. PMID: 33326020, PMCID: PMC7748821, DOI: 10.1084/jem.20200547.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody FormationAntigensB-LymphocytesCD4 AntigensDisease Models, AnimalEpitopesForkhead Transcription FactorsGerminal CenterHumansImmunityImmunologic MemoryInfluenza, HumanInfluenzavirus BIntegrasesMice, Inbred C57BLOrthomyxoviridae InfectionsReceptors, Antigen, B-CellSpecies SpecificityT-Lymphocytes, RegulatoryVaccinationConceptsB cell responsesGerminal center B cell responsesFollicular regulatory T cellsRegulatory T cellsTfr cellsCell responsesT cellsViral challengeHumoral memoryVirus-specific B cell responsesAntigen-specific B cell responsesFollicular helper T cellsHA stalk regionHelper T cellsInfluenza virus infectionGerminal center developmentAntibody responsePlasma cellsVirus infectionImmunization modelAntibody productionBCR repertoireInfluenza virusRepeated exposureInfluenza virus glycoproteins
2017
Interleukin-10 from CD4+ follicular regulatory T cells promotes the germinal center response
Laidlaw BJ, Lu Y, Amezquita RA, Weinstein JS, Vander Heiden JA, Gupta NT, Kleinstein SH, Kaech SM, Craft J. Interleukin-10 from CD4+ follicular regulatory T cells promotes the germinal center response. Science Immunology 2017, 2 PMID: 29054998, PMCID: PMC5846620, DOI: 10.1126/sciimmunol.aan4767.Peer-Reviewed Original ResearchConceptsFollicular regulatory T cellsRegulatory T cellsIL-10Lymphocytic choriomeningitis virusT cellsB cellsInterleukin-10GC responseCell-derived IL-10Follicular helper T cellsHelper T cellsB cell responsesGerminal center responseGerminal center developmentActivated B cellsBox protein 1GC B cellsAcute infectionCenter responseCell responsesImportant mediatorNuclear translocationGC reactionProtein 1Forkhead box protein 1Disruption of Pathogenic Cellular Networks by IL-21 Blockade Leads to Disease Amelioration in Murine Lupus.
Choi JY, Seth A, Kashgarian M, Terrillon S, Fung E, Huang L, Wang LC, Craft J. Disruption of Pathogenic Cellular Networks by IL-21 Blockade Leads to Disease Amelioration in Murine Lupus. The Journal Of Immunology 2017, 198: 2578-2588. PMID: 28219887, PMCID: PMC5360548, DOI: 10.4049/jimmunol.1601687.Peer-Reviewed Original ResearchConceptsIL-21Systemic lupus erythematosusMurine lupusTfh cellsLupus erythematosusDisease ameliorationGerminal center B cell responsesTfh-B cell interactionsGC responseFollicular Th cellsIL-21 blockadeLupus-prone B6Progression of glomerulonephritisCell-derived cytokinesB cell responsesMemory B cell developmentPotential therapeutic strategyB cell proliferationB cell maturationGC B cellsOverall survivalOrgan injuryAutoantibody productionLatter cytokineTh cells
2014
Aberant expansion of CXCR5+ memory CD4 T cells in SLE patients (HUM7P.307)
Choi J, Kim S, Kang I, Craft J. Aberant expansion of CXCR5+ memory CD4 T cells in SLE patients (HUM7P.307). The Journal Of Immunology 2014, 192: 184.16-184.16. DOI: 10.4049/jimmunol.192.supp.184.16.Peer-Reviewed Original ResearchCD4 T cellsSLE patientsT cellsTfh cellsB cellsB collaborationB cell helper functionFollicular helper T cellsMemory CD4 T cellsMemory Tfh cellsTh17-like subsetsAnti-nuclear antibodiesHelper T cellsB cell responsesHuman lupus patientsSecondary lymphoid organsMemory B cellsAutoreactive B cellsSuch cellsDistinct Th1Autoantibody titersDisease activityLupus patientsIL-21Lymphoid organs
2009
Bcl6 and Blimp-1 Are Reciprocal and Antagonistic Regulators of T Follicular Helper Cell Differentiation
Johnston RJ, Poholek AC, DiToro D, Yusuf I, Eto D, Barnett B, Dent AL, Craft J, Crotty S. Bcl6 and Blimp-1 Are Reciprocal and Antagonistic Regulators of T Follicular Helper Cell Differentiation. Science 2009, 325: 1006-1010. PMID: 19608860, PMCID: PMC2766560, DOI: 10.1126/science.1175870.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody FormationArenaviridae InfectionsB-LymphocytesCD4-Positive T-LymphocytesCell DifferentiationCell LineageCytokinesDNA-Binding ProteinsGene Expression RegulationGerminal CenterLymphocyte ActivationLymphocytic choriomeningitis virusMiceMice, Inbred C57BLMice, TransgenicPositive Regulatory Domain I-Binding Factor 1Proto-Oncogene Proteins c-bcl-6RNA, MessengerSignal TransductionT-Lymphocyte SubsetsT-Lymphocytes, Helper-InducerTranscription FactorsConceptsAntibody responseT cellsBlimp-1B cell germinal centersEffector T cell subsetsFollicular helper cell differentiationT Follicular Helper Cell DifferentiationFollicular helper cellsB cell-mediated immunityCell-mediated immunityT cell subsetsB cell responsesT cell helpTranscription factor Blimp-1Transcription factor Bcl6Helper cell differentiationDistinct CD4Cell subsetsHelper cellsCell helpGerminal centersB cellsCell responsesCD4BCL6
2008
CD4 T Cells That Promote Extrafollicular B Cell Responses
Poholek A, Odegard J, Craft J. CD4 T Cells That Promote Extrafollicular B Cell Responses. The FASEB Journal 2008, 22: 846.3-846.3. DOI: 10.1096/fasebj.22.1_supplement.846.3.Peer-Reviewed Original ResearchCD4 T cellsExtrafollicular B cell responsesB cell responsesIL-21T cellsLO cellsP-selectin glycoprotein ligand-1Extrafollicular sitesTCR transgenic CD4 T cellsCell responsesSelectin glycoprotein ligand-1Follicular helper T cellsTransgenic CD4 T cellsT FH cellsB cell folliclesHelper T cellsNormal immune responseExtrafollicular cellsAdoptive transferMurine lupusFH cellsIL-4Extrafollicular responseImmune responseNovel subset