2022
Tfh-cell-derived interleukin 21 sustains effector CD8+ T cell responses during chronic viral infection
Zander R, Kasmani MY, Chen Y, Topchyan P, Shen J, Zheng S, Burns R, Ingram J, Cui C, Joshi N, Craft J, Zajac A, Cui W. Tfh-cell-derived interleukin 21 sustains effector CD8+ T cell responses during chronic viral infection. Immunity 2022, 55: 475-493.e5. PMID: 35216666, PMCID: PMC8916994, DOI: 10.1016/j.immuni.2022.01.018.Peer-Reviewed Original ResearchConceptsChronic viral infectionsIL-21Cell responsesViral infectionMixed bone marrow chimera experimentsBone marrow chimera experimentsMemory-like subsetTfh cell responsesCell-mediated immunityTfh cellsEffector CD8LCMV infectionHelper subsetsInterleukin-21Th1 cellsViral controlCD8Chimera experimentsCD4InfectionCell differentiationCellsSubsetResponseDistinct populationsHigh-affinity, neutralizing antibodies to SARS-CoV-2 can be made without T follicular helper cells
Chen JS, Chow RD, Song E, Mao T, Israelow B, Kamath K, Bozekowski J, Haynes WA, Filler RB, Menasche BL, Wei J, Alfajaro MM, Song W, Peng L, Carter L, Weinstein JS, Gowthaman U, Chen S, Craft J, Shon JC, Iwasaki A, Wilen CB, Eisenbarth SC. High-affinity, neutralizing antibodies to SARS-CoV-2 can be made without T follicular helper cells. Science Immunology 2022, 7: eabl5652. PMID: 34914544, PMCID: PMC8977051, DOI: 10.1126/sciimmunol.abl5652.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionSARS-CoV-2Follicular helper cellsB cell responsesHelper cellsAntibody productionCell responsesSARS-CoV-2 vaccinationB-cell receptor sequencingSevere COVID-19Cell receptor sequencingIndependent antibodiesT cell-B cell interactionsViral inflammationAntiviral antibodiesImmunoglobulin class switchingVirus infectionGerminal centersViral infectionClonal repertoireInfectionAntibodiesClass switchingCOVID-19Patients
2021
Neoantigen-driven B cell and CD4 T follicular helper cell collaboration promotes anti-tumor CD8 T cell responses
Cui C, Wang J, Fagerberg E, Chen PM, Connolly KA, Damo M, Cheung JF, Mao T, Askari AS, Chen S, Fitzgerald B, Foster GG, Eisenbarth SC, Zhao H, Craft J, Joshi NS. Neoantigen-driven B cell and CD4 T follicular helper cell collaboration promotes anti-tumor CD8 T cell responses. Cell 2021, 184: 6101-6118.e13. PMID: 34852236, PMCID: PMC8671355, DOI: 10.1016/j.cell.2021.11.007.Peer-Reviewed Original ResearchConceptsCD8 TB cellsTfh cellsLung adenocarcinomaTfh-B cell interactionsTumor-specific B cellsFollicular helper cellsAnti-tumor immunityB cell signaturesCell effector functionsGerminal center formationGC B cellsCD4 THelper cellsTumor controlTumor neoantigensEffector functionsCell collaborationCell responsesCell signatureTumor cellsSignature correlatesNeoantigensCell functionCD4T Follicular Regulatory Cells: Choreographers of Productive Germinal Center Responses
Lu Y, Craft J. T Follicular Regulatory Cells: Choreographers of Productive Germinal Center Responses. Frontiers In Immunology 2021, 12: 679909. PMID: 34177925, PMCID: PMC8222975, DOI: 10.3389/fimmu.2021.679909.Peer-Reviewed Original ResearchConceptsTfr cellsTreg cellsHumoral immunityGerminal centersAntigen-specific B cell responsesFollicular helper cellsFollicular regulatory (Tfr) cellsB cell folliclesRegulatory T cellsB cell autoreactivityB cell responsesGerminal center responseB cell toleranceVaccine design strategiesTranscription factor Bcl6Regulatory cellsHelper cellsImmune homeostasisProductive immunityT cellsViral infectionCenter responseCell responsesGC responseImmune challengeNeoantigen driven B cell and CD4+ T follicular helper cell collaboration promotes robust anti-tumor CD8+ T cell responses
Cui C, Joshi N, Craft J. Neoantigen driven B cell and CD4+ T follicular helper cell collaboration promotes robust anti-tumor CD8+ T cell responses. The Journal Of Immunology 2021, 206: 57.01-57.01. DOI: 10.4049/jimmunol.206.supp.57.01.Peer-Reviewed Original ResearchT cell responsesAnti-tumor CD8Tfh cellsB cellsCell responsesIL-21Cell collaborationProtective anti-tumor responsesTfh-B cell interactionsTumor-specific B cellsGC B cell responsesTumor-specific CD4Anti-tumor immunityFavorable clinical outcomeAnti-tumor responseIL-21 receptorB cell responsesLung adenocarcinoma patientsAnalysis of survivalT cell-B cell interactionsGerminal center formationCell interactionsGC B cellsAbstract CD4Effector CD8
2020
CD4+ follicular regulatory T cells optimize the influenza virus–specific B cell response
Lu Y, Jiang R, Freyn AW, Wang J, Strohmeier S, Lederer K, Locci M, Zhao H, Angeletti D, O’Connor K, Kleinstein SH, Nachbagauer R, Craft J. CD4+ follicular regulatory T cells optimize the influenza virus–specific B cell response. Journal Of Experimental Medicine 2020, 218: e20200547. PMID: 33326020, PMCID: PMC7748821, DOI: 10.1084/jem.20200547.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody FormationAntigensB-LymphocytesCD4 AntigensDisease Models, AnimalEpitopesForkhead Transcription FactorsGerminal CenterHumansImmunityImmunologic MemoryInfluenza, HumanInfluenzavirus BIntegrasesMice, Inbred C57BLOrthomyxoviridae InfectionsReceptors, Antigen, B-CellSpecies SpecificityT-Lymphocytes, RegulatoryVaccinationConceptsB cell responsesGerminal center B cell responsesFollicular regulatory T cellsRegulatory T cellsTfr cellsCell responsesT cellsViral challengeHumoral memoryVirus-specific B cell responsesAntigen-specific B cell responsesFollicular helper T cellsHA stalk regionHelper T cellsInfluenza virus infectionGerminal center developmentAntibody responsePlasma cellsVirus infectionImmunization modelAntibody productionBCR repertoireInfluenza virusRepeated exposureInfluenza virus glycoproteins
2018
Single-cell RNA sequencing unveils an IL-10-producing helper subset that sustains humoral immunity during persistent infection
Xin G, Zander R, Schauder DM, Chen Y, Weinstein JS, Drobyski WR, Tarakanova V, Craft J, Cui W. Single-cell RNA sequencing unveils an IL-10-producing helper subset that sustains humoral immunity during persistent infection. Nature Communications 2018, 9: 5037. PMID: 30487586, PMCID: PMC6261948, DOI: 10.1038/s41467-018-07492-4.Peer-Reviewed Original ResearchConceptsCD4 T cellsIL-10Humoral immunityT cellsTfh cellsIL-10-expressing CD4 T cellsViral infectionAntiviral T cell responsesAcute LCMV infectionDouble reporter miceChronic viral infectionsCytokine IL-10T cell responsesPersistent viral infectionIL-10 signalingGerminal center reactionSingle-cell RNA-sequencing approachLCMV infectionHelper subsetsTh1 cellsChronic infectionInflammatory functionsViral controlPersistent infectionCell responses
2017
Interleukin-10 from CD4+ follicular regulatory T cells promotes the germinal center response
Laidlaw BJ, Lu Y, Amezquita RA, Weinstein JS, Vander Heiden JA, Gupta NT, Kleinstein SH, Kaech SM, Craft J. Interleukin-10 from CD4+ follicular regulatory T cells promotes the germinal center response. Science Immunology 2017, 2 PMID: 29054998, PMCID: PMC5846620, DOI: 10.1126/sciimmunol.aan4767.Peer-Reviewed Original ResearchConceptsFollicular regulatory T cellsRegulatory T cellsIL-10Lymphocytic choriomeningitis virusT cellsB cellsInterleukin-10GC responseCell-derived IL-10Follicular helper T cellsHelper T cellsB cell responsesGerminal center responseGerminal center developmentActivated B cellsBox protein 1GC B cellsAcute infectionCenter responseCell responsesImportant mediatorNuclear translocationGC reactionProtein 1Forkhead box protein 1
2016
The multifaceted role of CD4+ T cells in CD8+ T cell memory
Laidlaw BJ, Craft JE, Kaech SM. The multifaceted role of CD4+ T cells in CD8+ T cell memory. Nature Reviews Immunology 2016, 16: 102-111. PMID: 26781939, PMCID: PMC4860014, DOI: 10.1038/nri.2015.10.Peer-Reviewed Original ResearchConceptsT cell responsesT cell poolDendritic cellsT cell helpT cellsMemory CD8Cell helpCell responsesSuppression of DCTissue-resident memory CD8Regulatory T cellsT cell memoryCell poolSuppression of TNFApoptosis-inducing ligandEffector CD4Treg cellsFunctional CD8IL-21Primary CD8Protective immunityInterleukin-15Secondary responsivenessChronic infectionIL-2
2010
Dysregulated balance of Th17 and Th1 cells in systemic lupus erythematosus
Shah K, Lee WW, Lee SH, Kim SH, Kang SW, Craft J, Kang I. Dysregulated balance of Th17 and Th1 cells in systemic lupus erythematosus. Arthritis Research & Therapy 2010, 12: r53. PMID: 20334681, PMCID: PMC2888202, DOI: 10.1186/ar2964.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusSLE Disease Activity Index (SLEDAI) scorePeripheral blood mononuclear cellsTh17-polarizing cytokinesTh1 cell responsesDisease activityT cellsHealthy subjectsIL-17Th1 cellsCell responsesPhorbol myristate acetateTh17 cellsLupus erythematosusIL-6Plasma levelsDisease activity index scoreBalance of CD4Balance of Th17Expression of Th17Th17 cell responseActivity index scoreFrequency of CD4Chemokine receptor CCR4Blood mononuclear cells
2009
Bcl6 and Blimp-1 Are Reciprocal and Antagonistic Regulators of T Follicular Helper Cell Differentiation
Johnston RJ, Poholek AC, DiToro D, Yusuf I, Eto D, Barnett B, Dent AL, Craft J, Crotty S. Bcl6 and Blimp-1 Are Reciprocal and Antagonistic Regulators of T Follicular Helper Cell Differentiation. Science 2009, 325: 1006-1010. PMID: 19608860, PMCID: PMC2766560, DOI: 10.1126/science.1175870.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody FormationArenaviridae InfectionsB-LymphocytesCD4-Positive T-LymphocytesCell DifferentiationCell LineageCytokinesDNA-Binding ProteinsGene Expression RegulationGerminal CenterLymphocyte ActivationLymphocytic choriomeningitis virusMiceMice, Inbred C57BLMice, TransgenicPositive Regulatory Domain I-Binding Factor 1Proto-Oncogene Proteins c-bcl-6RNA, MessengerSignal TransductionT-Lymphocyte SubsetsT-Lymphocytes, Helper-InducerTranscription FactorsConceptsAntibody responseT cellsBlimp-1B cell germinal centersEffector T cell subsetsFollicular helper cell differentiationT Follicular Helper Cell DifferentiationFollicular helper cellsB cell-mediated immunityCell-mediated immunityT cell subsetsB cell responsesT cell helpTranscription factor Blimp-1Transcription factor Bcl6Helper cell differentiationDistinct CD4Cell subsetsHelper cellsCell helpGerminal centersB cellsCell responsesCD4BCL6
2008
CD4 T Cells That Promote Extrafollicular B Cell Responses
Poholek A, Odegard J, Craft J. CD4 T Cells That Promote Extrafollicular B Cell Responses. The FASEB Journal 2008, 22: 846.3-846.3. DOI: 10.1096/fasebj.22.1_supplement.846.3.Peer-Reviewed Original ResearchCD4 T cellsExtrafollicular B cell responsesB cell responsesIL-21T cellsLO cellsP-selectin glycoprotein ligand-1Extrafollicular sitesTCR transgenic CD4 T cellsCell responsesSelectin glycoprotein ligand-1Follicular helper T cellsTransgenic CD4 T cellsT FH cellsB cell folliclesHelper T cellsNormal immune responseExtrafollicular cellsAdoptive transferMurine lupusFH cellsIL-4Extrafollicular responseImmune responseNovel subset
2004
Association of reduced CD4 T cell responses specific to varicella zoster virus with high incidence of herpes zoster in patients with systemic lupus erythematosus.
Park HB, Kim KC, Park JH, Kang TY, Lee HS, Kim TH, Jun JB, Bae SC, Yoo DH, Craft J, Jung S. Association of reduced CD4 T cell responses specific to varicella zoster virus with high incidence of herpes zoster in patients with systemic lupus erythematosus. The Journal Of Rheumatology 2004, 31: 2151-5. PMID: 15517626.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusVaricella-zoster virusCD4 T cell responsesT cell frequenciesT cell responsesCD4 T cellsHerpes zosterT cellsHigh incidenceLupus erythematosusZoster virusVZV-specific CD4 T cellsCell frequencyCD4 T-cell frequenciesCell responsesMemory T cellsFlow cytometry analysisVZV reactivationVZV stimulationDisease activityIL-10VZV antigenWhole blood samplesClinical statusRisk factorsResistance to Development of Collagen-Induced Arthritis in C57BL/6 Mice Is Due to a Defect in Secondary, but Not in Primary, Immune Response
Pan M, Kang I, Craft J, Yin Z. Resistance to Development of Collagen-Induced Arthritis in C57BL/6 Mice Is Due to a Defect in Secondary, but Not in Primary, Immune Response. Journal Of Clinical Immunology 2004, 24: 481-491. PMID: 15359107, DOI: 10.1023/b:joci.0000040919.16739.44.Peer-Reviewed Original ResearchConceptsCollagen-induced arthritisB6 miceImmune responseSimilar T cell proliferationBovine type II collagenB cell immune responsesH-2b backgroundAnti-CII antibodiesHuman rheumatoid arthritisT cell responsesCell immune responsesSecondary immune responseT cell proliferationCII AbsCytokine responsesRheumatoid arthritisInitial immunizationC57BL/6 miceRodent modelsArthritisType II collagenDay 14Cell responsesDay 12MiceAge-Associated Change in the Frequency of Memory CD4+ T Cells Impairs Long Term CD4+ T Cell Responses to Influenza Vaccine
Kang I, Hong MS, Nolasco H, Park SH, Dan JM, Choi JY, Craft J. Age-Associated Change in the Frequency of Memory CD4+ T Cells Impairs Long Term CD4+ T Cell Responses to Influenza Vaccine. The Journal Of Immunology 2004, 173: 673-681. PMID: 15210831, DOI: 10.4049/jimmunol.173.1.673.Peer-Reviewed Original ResearchConceptsT cell responsesIL-7 levelsMemory CD4Long-term CD4T cellsCell responsesInfluenza vaccineIFN-gammaInfluenza virus-specific CD4Serum IL-7 levelsEM cellsLevels of CD4Virus-specific CD4Central memory CD4Effector memory CD4Cellular immune responsesFrequency of influenzaImportant clinical questionsAge-Associated ChangesEM CD4Influenza vaccinationTNF-alphaVaccination strategiesIL-7Immune responseDefective Control of Latent Epstein-Barr Virus Infection in Systemic Lupus Erythematosus
Kang I, Quan T, Nolasco H, Park SH, Hong MS, Crouch J, Pamer EG, Howe JG, Craft J. Defective Control of Latent Epstein-Barr Virus Infection in Systemic Lupus Erythematosus. The Journal Of Immunology 2004, 172: 1287-1294. PMID: 14707107, DOI: 10.4049/jimmunol.172.2.1287.Peer-Reviewed Original ResearchMeSH KeywordsAdultB-Lymphocyte SubsetsCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCytomegalovirusEpitopes, T-LymphocyteEpstein-Barr Virus InfectionsFemaleHerpesvirus 4, HumanHumansLeukocytes, MononuclearLupus Erythematosus, SystemicLymphocyte CountMaleMiddle AgedSeverity of Illness IndexViral LoadVirus LatencyConceptsSystemic lupus erythematosusEBV viral loadT cell responsesViral loadT cellsIFN-gammaCell responsesEBV infectionLupus erythematosusHealthy controlsEBV-specific T-cell responsesVirus-specific T cell responsesLatent Epstein-Barr virus (EBV) infectionEBV-specific immune responsesEpstein-Barr virus infectionAltered T-cell responsesDefective controlFrequency of CD69HLA-A2 tetramersTetramer-positive CD8Latent EBV infectionEBV-specificImmunosuppressive medicationsDisease activityLupus patients
1998
Influence of antigen organization on the development of lupus autoantibodies
Fatenejad S, Bennett M, Moslehi J, Craft J. Influence of antigen organization on the development of lupus autoantibodies. Arthritis & Rheumatism 1998, 41: 603-612. PMID: 9550469, DOI: 10.1002/1529-0131(199804)41:4<603::aid-art7>3.0.co;2-e.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAutoantibodiesAutoantigensGene ExpressionHeLa CellsHumansImmune ToleranceImmunizationLupus Erythematosus, SystemicMiceMice, Inbred C57BLMice, Inbred StrainsRecombinant Fusion ProteinsRecombinant ProteinsRibonucleoprotein, U1 Small NuclearRibonucleoproteins, Small NuclearRNA-Binding ProteinsT-LymphocytesTumor Cells, CulturedConceptsSystemic lupus erythematosusDevelopment of antibodiesH-2b backgroundDevelopment of lupusT cell responsesT cell toleranceAssessment of antibodiesNative snRNP particlesTypical immune responseLupus erythematosusAutoimmune responseNormal miceAntigen organizationImmune responseForeign immunogensH-2kCell toleranceMajor autoantigenCell responsesMiceRandom orderAntibodiesEukaryotic expression vectorLupusAutoantigens
1994
B cells process and present lupus autoantigens that initiate autoimmune T cell responses.
Mamula MJ, Fatenejad S, Craft J. B cells process and present lupus autoantigens that initiate autoimmune T cell responses. The Journal Of Immunology 1994, 152: 1453-61. PMID: 8301145, DOI: 10.4049/jimmunol.152.3.1453.Peer-Reviewed Original ResearchConceptsT cell responsesAutoimmune T cell responsesT cellsCell responsesB cellsAnti-snRNP antibodiesCross-reactive B cellsNon-autoimmune miceT cell toleranceB-cell processesSera of humansCross-reactive determinantsNative snRNP particlesMouse AgAutoreactive CD4Naive miceAutoantibody responseAutoreactive cellsMurine lupusTh cellsCommon findingSelf-AgCell toleranceMolecular mimicryMice