2023
AP-1–independent NFAT signaling maintains follicular T cell function in infection and autoimmunity
Seth A, Yokokura Y, Choi J, Shyer J, Vidyarthi A, Craft J. AP-1–independent NFAT signaling maintains follicular T cell function in infection and autoimmunity. Journal Of Experimental Medicine 2023, 220: e20211110. PMID: 36820828, PMCID: PMC9998660, DOI: 10.1084/jem.20211110.Peer-Reviewed Original ResearchConceptsTfh cellsT cellsFollicular helper T cellsLupus-prone miceT cell subsetsTfh cell developmentHelper T cellsHumoral immune responseT cell functionGerminal center B cellsT cell statesRenal injuryAutoantibody productionCell subsetsPrimary T cellsImmune responseB cellsPharmacologic inhibitionTherapeutic insightsCell functionGenetic disruptionNFATCell developmentCellsGene expression
2022
Development of Tbet- and CD11c-expressing B cells in a viral infection requires T follicular helper cells outside of germinal centers
Song W, Antao OQ, Condiff E, Sanchez GM, Chernova I, Zembrzuski K, Steach H, Rubtsova K, Angeletti D, Lemenze A, Laidlaw BJ, Craft J, Weinstein JS. Development of Tbet- and CD11c-expressing B cells in a viral infection requires T follicular helper cells outside of germinal centers. Immunity 2022, 55: 290-307.e5. PMID: 35090581, PMCID: PMC8965751, DOI: 10.1016/j.immuni.2022.01.002.Peer-Reviewed Original ResearchConceptsFollicular helper cellsB cellsGC B cellsHelper cellsMemory subsetsRobust recall responsesB cell subsetsGerminal center formationB cell generationAcute infectionCell subsetsRecall responsesTh1 cellsProximal deliveryCD11cGerminal centersIntegrin LFA-1Viral infectionAntibody productionVLA-4Splenic retentionLFA-1TbetBCL6 expressionInfection
2019
Identification of a T follicular helper cell subset that drives anaphylactic IgE
Gowthaman U, Chen JS, Zhang B, Flynn WF, Lu Y, Song W, Joseph J, Gertie JA, Xu L, Collet MA, Grassmann JDS, Simoneau T, Chiang D, Berin MC, Craft JE, Weinstein JS, Williams A, Eisenbarth SC. Identification of a T follicular helper cell subset that drives anaphylactic IgE. Science 2019, 365 PMID: 31371561, PMCID: PMC6901029, DOI: 10.1126/science.aaw6433.Peer-Reviewed Original ResearchConceptsInterleukin-4B cellsFollicular Helper Cell SubsetsLow-affinity IgEFollicular helper cellsAllergen-specific IgEHelper cell subsetsIsotypes of antibodiesAlternative therapeutic targetsTranscription factor Bcl6Anaphylactic IgECytokine profileIgE productionCell subsetsHelper cellsImmunoglobulin ETherapeutic targetIgEAnaphylaxisAllergensCellular mechanismsRare populationCellsMiceGATA3
2018
Human Extrafollicular CD4+ Th Cells Help Memory B Cells Produce Igs.
Kim ST, Choi JY, Lainez B, Schulz VP, Karas DE, Baum ED, Setlur J, Gallagher PG, Craft J. Human Extrafollicular CD4+ Th Cells Help Memory B Cells Produce Igs. The Journal Of Immunology 2018, 201: 1359-1372. PMID: 30030323, PMCID: PMC6112860, DOI: 10.4049/jimmunol.1701217.Peer-Reviewed Original ResearchConceptsMemory B cellsHumoral recall responsesB cell folliclesT cell subsetsB cellsTfh cellsCell subsetsRecall responsesT cellsInhibitory receptor PD-1Follicular helper T cellsGerminal center B-cell maturationIL-21 secretionChemokine receptor expressionReceptor PD-1Helper T cellsT cell zonesPrimary immune responseChemokine receptor CXCR5Transcription factor Bcl6B cell maturationTonsillar CD4IL-10IL-21PD-1
2015
The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells
Ray JP, Staron MM, Shyer JA, Ho PC, Marshall HD, Gray SM, Laidlaw BJ, Araki K, Ahmed R, Kaech SM, Craft J. The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells. Immunity 2015, 43: 690-702. PMID: 26410627, PMCID: PMC4618086, DOI: 10.1016/j.immuni.2015.08.017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCalcium SignalingCell CycleCell DivisionEnzyme ActivationGlucoseGlycolysisInterleukin-2Interleukin-2 Receptor alpha SubunitLymphocytic choriomeningitis virusMechanistic Target of Rapamycin Complex 1Mice, Inbred C57BLMultiprotein ComplexesNFATC Transcription FactorsOxygen ConsumptionPositive Regulatory Domain I-Binding Factor 1Proto-Oncogene Proteins c-aktSignal TransductionSpecific Pathogen-Free OrganismsTh1 CellsT-Lymphocyte SubsetsT-Lymphocytes, Helper-InducerTOR Serine-Threonine KinasesTranscription FactorsConceptsFollicular B helper T cellsAcute viral infectionHelper T cellsTfh cellsT cellsViral infectionT helper 1 cellsHelper T cell subsetsIL-2-mediated activationT cell subsetsDiverse effector functionsT helper 1Dependent cytokine productionTh1 cell fateHelper 1Cell subsetsCytokine productionTh1 cellsInterleukin-2Effector functionsCritical orchestratorsMTOR kinase activityLess proliferationMTORC1 axisReciprocal balance
2011
Expansion of IL-7Ralow memory CD8 T cells in human lupus (101.19)
Lee N, Shah K, Shin M, Kim S, Craft J, Kang I. Expansion of IL-7Ralow memory CD8 T cells in human lupus (101.19). The Journal Of Immunology 2011, 186: 101.19-101.19. DOI: 10.4049/jimmunol.186.supp.101.19.Peer-Reviewed Original ResearchSystemic lupus erythematosusDevelopment of SLESLE Disease Activity IndexMemory CD8SLE patientsT cellsCell subsetsHealthy controlsImmunopathogenesis of SLEAbstract Systemic lupus erythematosusHomeostatic cytokine IL-15IL-7 receptor alphaMemory CD8 T cellsCytotoxic molecules perforinHomeostasis of CD8Disease activity indexRole of CD8Cytokine IL-15CD8 T cellsImmune complex depositionProduction of autoantibodiesInflammatory autoimmune disorderT cell receptorDisease activityLupus erythematosus
2009
Thymic self-reactivity selects natural interleukin 17–producing T cells that can regulate peripheral inflammation
Marks BR, Nowyhed HN, Choi JY, Poholek AC, Odegard JM, Flavell RA, Craft J. Thymic self-reactivity selects natural interleukin 17–producing T cells that can regulate peripheral inflammation. Nature Immunology 2009, 10: 1125-1132. PMID: 19734905, PMCID: PMC2751862, DOI: 10.1038/ni.1783.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutoantigensCD4-Positive T-LymphocytesCell DifferentiationEnzyme-Linked Immunosorbent AssayFlow CytometryInflammationIntegrin alpha4beta1Interleukin-17Interleukin-23Interleukin-6InterleukinsMiceMice, TransgenicNuclear Receptor Subfamily 1, Group F, Member 3Polymerase Chain ReactionReceptors, CCR6Receptors, Retinoic AcidReceptors, Thyroid HormoneThymus GlandT-Lymphocyte SubsetsTransforming Growth Factor betaBcl6 and Blimp-1 Are Reciprocal and Antagonistic Regulators of T Follicular Helper Cell Differentiation
Johnston RJ, Poholek AC, DiToro D, Yusuf I, Eto D, Barnett B, Dent AL, Craft J, Crotty S. Bcl6 and Blimp-1 Are Reciprocal and Antagonistic Regulators of T Follicular Helper Cell Differentiation. Science 2009, 325: 1006-1010. PMID: 19608860, PMCID: PMC2766560, DOI: 10.1126/science.1175870.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody FormationArenaviridae InfectionsB-LymphocytesCD4-Positive T-LymphocytesCell DifferentiationCell LineageCytokinesDNA-Binding ProteinsGene Expression RegulationGerminal CenterLymphocyte ActivationLymphocytic choriomeningitis virusMiceMice, Inbred C57BLMice, TransgenicPositive Regulatory Domain I-Binding Factor 1Proto-Oncogene Proteins c-bcl-6RNA, MessengerSignal TransductionT-Lymphocyte SubsetsT-Lymphocytes, Helper-InducerTranscription FactorsConceptsAntibody responseT cellsBlimp-1B cell germinal centersEffector T cell subsetsFollicular helper cell differentiationT Follicular Helper Cell DifferentiationFollicular helper cellsB cell-mediated immunityCell-mediated immunityT cell subsetsB cell responsesT cell helpTranscription factor Blimp-1Transcription factor Bcl6Helper cell differentiationDistinct CD4Cell subsetsHelper cellsCell helpGerminal centersB cellsCell responsesCD4BCL6