2018
Single-cell RNA sequencing unveils an IL-10-producing helper subset that sustains humoral immunity during persistent infection
Xin G, Zander R, Schauder DM, Chen Y, Weinstein JS, Drobyski WR, Tarakanova V, Craft J, Cui W. Single-cell RNA sequencing unveils an IL-10-producing helper subset that sustains humoral immunity during persistent infection. Nature Communications 2018, 9: 5037. PMID: 30487586, PMCID: PMC6261948, DOI: 10.1038/s41467-018-07492-4.Peer-Reviewed Original ResearchConceptsCD4 T cellsIL-10Humoral immunityT cellsTfh cellsIL-10-expressing CD4 T cellsViral infectionAntiviral T cell responsesAcute LCMV infectionDouble reporter miceChronic viral infectionsCytokine IL-10T cell responsesPersistent viral infectionIL-10 signalingGerminal center reactionSingle-cell RNA-sequencing approachLCMV infectionHelper subsetsTh1 cellsChronic infectionInflammatory functionsViral controlPersistent infectionCell responsesHuman Extrafollicular CD4+ Th Cells Help Memory B Cells Produce Igs.
Kim ST, Choi JY, Lainez B, Schulz VP, Karas DE, Baum ED, Setlur J, Gallagher PG, Craft J. Human Extrafollicular CD4+ Th Cells Help Memory B Cells Produce Igs. The Journal Of Immunology 2018, 201: 1359-1372. PMID: 30030323, PMCID: PMC6112860, DOI: 10.4049/jimmunol.1701217.Peer-Reviewed Original ResearchConceptsMemory B cellsHumoral recall responsesB cell folliclesT cell subsetsB cellsTfh cellsCell subsetsRecall responsesT cellsInhibitory receptor PD-1Follicular helper T cellsGerminal center B-cell maturationIL-21 secretionChemokine receptor expressionReceptor PD-1Helper T cellsT cell zonesPrimary immune responseChemokine receptor CXCR5Transcription factor Bcl6B cell maturationTonsillar CD4IL-10IL-21PD-1
2017
Interleukin-10 from CD4+ follicular regulatory T cells promotes the germinal center response
Laidlaw BJ, Lu Y, Amezquita RA, Weinstein JS, Vander Heiden JA, Gupta NT, Kleinstein SH, Kaech SM, Craft J. Interleukin-10 from CD4+ follicular regulatory T cells promotes the germinal center response. Science Immunology 2017, 2 PMID: 29054998, PMCID: PMC5846620, DOI: 10.1126/sciimmunol.aan4767.Peer-Reviewed Original ResearchConceptsFollicular regulatory T cellsRegulatory T cellsIL-10Lymphocytic choriomeningitis virusT cellsB cellsInterleukin-10GC responseCell-derived IL-10Follicular helper T cellsHelper T cellsB cell responsesGerminal center responseGerminal center developmentActivated B cellsBox protein 1GC B cellsAcute infectionCenter responseCell responsesImportant mediatorNuclear translocationGC reactionProtein 1Forkhead box protein 1
2015
Production of IL-10 by CD4+ regulatory T cells during the resolution of infection promotes the maturation of memory CD8+ T cells
Laidlaw BJ, Cui W, Amezquita RA, Gray SM, Guan T, Lu Y, Kobayashi Y, Flavell RA, Kleinstein SH, Craft J, Kaech SM. Production of IL-10 by CD4+ regulatory T cells during the resolution of infection promotes the maturation of memory CD8+ T cells. Nature Immunology 2015, 16: 871-879. PMID: 26147684, PMCID: PMC4713030, DOI: 10.1038/ni.3224.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAnimalsCD8-Positive T-LymphocytesDendritic CellsFlow CytometryGene Expression ProfilingHost-Pathogen InteractionsImmunologic MemoryInflammationInterleukin-10Lymphocytic ChoriomeningitisLymphocytic choriomeningitis virusMice, Inbred C57BLMice, KnockoutReverse Transcriptase Polymerase Chain ReactionT-Lymphocytes, Regulatory
2012
IL-10-IL-21-STAT3 axis regulates memory CD8 T cell development (110.9)
Cui W, Liu Y, Weinstein J, Craft J, Kaech S. IL-10-IL-21-STAT3 axis regulates memory CD8 T cell development (110.9). The Journal Of Immunology 2012, 188: 110.9-110.9. DOI: 10.4049/jimmunol.188.supp.110.9.Peer-Reviewed Original ResearchCD8 T cellsMemory T cellsMemory CD8 T cell developmentMemory CD8 T cellsCD8 T cell developmentT cellsT cell developmentIL-21IL-10Virus-specific CD8 T cellsBCL-6Effector CD8 T cellsProtective memory T cellsCentral memory T cellsMemory CD8 T cell differentiationCD8 T cell differentiationAcute LCMV infectionMemory precursor cellsCell developmentMemory cell developmentSTAT3-SOCS3 pathwayT cell differentiationAcute infectionLCMV infectionIL-12
2008
The Role of ICOS in Peripheral Inflammation in Lupus
Eardley L, Odegard J, Craft J. The Role of ICOS in Peripheral Inflammation in Lupus. The FASEB Journal 2008, 22: 668.18-668.18. DOI: 10.1096/fasebj.22.1_supplement.668.18.Peer-Reviewed Original ResearchCostimulatory molecule ICOST cellsPeripheral inflammationDendritic cellsChemokine receptorsMRL/Faslpr miceExpression of CCR6Role of ICOSChemokine receptor expressionMajority of CD4Role of CD4Lupus-prone miceSevere renal diseaseFaslpr miceIndependent cytokinesLupus nephritisEffector cytokinesIL-10Lupus miceRenal diseaseCellular infiltrateChemokine productionSuch inflammationIL-5Certain chemokines
2004
Association of reduced CD4 T cell responses specific to varicella zoster virus with high incidence of herpes zoster in patients with systemic lupus erythematosus.
Park HB, Kim KC, Park JH, Kang TY, Lee HS, Kim TH, Jun JB, Bae SC, Yoo DH, Craft J, Jung S. Association of reduced CD4 T cell responses specific to varicella zoster virus with high incidence of herpes zoster in patients with systemic lupus erythematosus. The Journal Of Rheumatology 2004, 31: 2151-5. PMID: 15517626.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusVaricella-zoster virusCD4 T cell responsesT cell frequenciesT cell responsesCD4 T cellsHerpes zosterT cellsHigh incidenceLupus erythematosusZoster virusVZV-specific CD4 T cellsCell frequencyCD4 T-cell frequenciesCell responsesMemory T cellsFlow cytometry analysisVZV reactivationVZV stimulationDisease activityIL-10VZV antigenWhole blood samplesClinical statusRisk factors
2002
IL-10 Regulates Murine Lupus
Yin Z, Bahtiyar G, Zhang N, Liu L, Zhu P, Robert ME, McNiff J, Madaio MP, Craft J. IL-10 Regulates Murine Lupus. The Journal Of Immunology 2002, 169: 2148-2155. PMID: 12165544, DOI: 10.4049/jimmunol.169.4.2148.Peer-Reviewed Original ResearchConceptsIL-10Murine lupusHuman systemic lupus erythematosusAnti-dsDNA autoantibody productionSeverity of lupusTh1 cytokine responseAnti-dsDNA autoantibodiesSystemic lupus erythematosusIFN-gamma productionIL-10 locusMRL/MpJPotential therapeutic benefitLupus syndromeSevere glomerulonephritisSevere lupusLupus modelsLupus erythematosusRIL-10Autoantibody productionCytokine responsesHuman lupusSerum concentrationsProtective effectSkin lesionsLittermate controlsTransgenic Overexpression of Interleukin (IL)-10 in the Lung Causes Mucus Metaplasia, Tissue Inflammation, and Airway Remodeling via IL-13-dependent and -independent Pathways*
Lee CG, Homer RJ, Cohn L, Link H, Jung S, Craft JE, Graham BS, Johnson TR, Elias JA. Transgenic Overexpression of Interleukin (IL)-10 in the Lung Causes Mucus Metaplasia, Tissue Inflammation, and Airway Remodeling via IL-13-dependent and -independent Pathways*. Journal Of Biological Chemistry 2002, 277: 35466-35474. PMID: 12107190, DOI: 10.1074/jbc.m206395200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceChloride ChannelsCloning, MolecularDNA PrimersFluorescent Antibody TechniqueGene Expression RegulationIn Situ HybridizationInflammationInterleukin-10Interleukin-13LungMiceMice, TransgenicMolecular Sequence DataMucoproteinsMucous MembranePhenotypePolymerase Chain ReactionReceptors, Interleukin-4STAT6 Transcription FactorTrans-ActivatorsConceptsMucus metaplasiaIL-10Tissue inflammationIL-13Tumor necrosis factor productionIL-13/ILLipopolysaccharide-induced inflammationNecrosis factor productionAirway fibrosisNeutrophil accumulationAirway remodelingSubepithelial fibrosisGob-5Levels of mRNAMetaplasiaInflammationTransgenic miceFibrosisSTAT-6Effector propertiesTransgenic overexpressionFactor productionMiceInterleukinMultiple mechanisms