2023
Bulk and single-nucleus RNA sequencing highlight immune pathways induced in individuals during an Ixodes scapularis tick bite
Tang X, Lynn G, Cui Y, Cerny J, Arora G, Tomayko M, Craft J, Fikrig E. Bulk and single-nucleus RNA sequencing highlight immune pathways induced in individuals during an Ixodes scapularis tick bite. Infection And Immunity 2023, 91: e00282-23. PMID: 37846980, PMCID: PMC10652856, DOI: 10.1128/iai.00282-23.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceFeeding BehaviorGuinea PigsHumansIxodesRNA, Small NuclearTick BitesConceptsRNA sequencingSingle-nucleus RNA sequencingBulk RNA sequencingInterleukin-17 signalingPlatelet activation pathwaysLaboratory guinea pigsSnRNA-seqHippo signalingIndividual genesPeripheral bloodTick biteAdaptive immunityAnti-tick vaccinesGuidance pathwayImmune pathwaysNew biomarkersHost responseGuinea pigsHematophagous arthropodsHost defenseCell adhesionTick attachmentNovel insightsTick feedingPhysiological consequences
2005
Role for macrophage migration inhibitory factor in asthma
Mizue Y, Ghani S, Leng L, McDonald C, Kong P, Baugh J, Lane SJ, Craft J, Nishihira J, Donnelly SC, Zhu Z, Bucala R. Role for macrophage migration inhibitory factor in asthma. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 14410-14415. PMID: 16186482, PMCID: PMC1242335, DOI: 10.1073/pnas.0507189102.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAsthmaBase SequenceBronchoalveolar LavageCell ProliferationCells, CulturedCytokinesEnzyme-Linked Immunosorbent AssayFlow CytometryGene ComponentsGenotypeHumansInterleukin-5Intramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsMiceMice, Inbred BALB CMice, KnockoutOvalbuminReverse Transcriptase Polymerase Chain ReactionT-Lymphocytes, Helper-InducerConceptsMacrophage migration inhibitory factorMigration inhibitory factorInhibitory factorIL-5 concentrationsLower airway hyperresponsivenessBronchoalveolar lavage fluidAntigen-specific responsesT-cell studiesWild-type miceLess pulmonary inflammationAirway hyperresponsivenessImmunologic regulatorsMIF deficiencyMIF KOMIF's roleMild asthmaWild-type controlsSevere asthmaAsthma patientsCytokine levelsMIF genotypePulmonary inflammationSpecific IgEWhite patientsAutoimmune disorders
2003
Age-dependent Requirement for γδ T Cells in the Primary but Not Secondary Protective Immune Response against an Intestinal Parasite
Ramsburg E, Tigelaar R, Craft J, Hayday A. Age-dependent Requirement for γδ T Cells in the Primary but Not Secondary Protective Immune Response against an Intestinal Parasite. Journal Of Experimental Medicine 2003, 198: 1403-1414. PMID: 14597739, PMCID: PMC2194243, DOI: 10.1084/jem.20030050.Peer-Reviewed Original ResearchConceptsAlphabeta T cellsGammadelta cellsT cellsYoung miceImmune responseAdult miceIntestinal parasitesPathogen-specific immunityΓδ T cellsCellular immune responsesProtective immune responseT cell receptorWk of ageAdoptive transferDevelopment of resistanceImmune protectionNewborn recipientsEimeria vermiformisAge-dependent requirementsCell receptorMiceE. vermiformisLymphoid progenitorsInfectionYoung hosts
2002
Transgenic Overexpression of Interleukin (IL)-10 in the Lung Causes Mucus Metaplasia, Tissue Inflammation, and Airway Remodeling via IL-13-dependent and -independent Pathways*
Lee CG, Homer RJ, Cohn L, Link H, Jung S, Craft JE, Graham BS, Johnson TR, Elias JA. Transgenic Overexpression of Interleukin (IL)-10 in the Lung Causes Mucus Metaplasia, Tissue Inflammation, and Airway Remodeling via IL-13-dependent and -independent Pathways*. Journal Of Biological Chemistry 2002, 277: 35466-35474. PMID: 12107190, DOI: 10.1074/jbc.m206395200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceChloride ChannelsCloning, MolecularDNA PrimersFluorescent Antibody TechniqueGene Expression RegulationIn Situ HybridizationInflammationInterleukin-10Interleukin-13LungMiceMice, TransgenicMolecular Sequence DataMucoproteinsMucous MembranePhenotypePolymerase Chain ReactionReceptors, Interleukin-4STAT6 Transcription FactorTrans-ActivatorsConceptsMucus metaplasiaIL-10Tissue inflammationIL-13Tumor necrosis factor productionIL-13/ILLipopolysaccharide-induced inflammationNecrosis factor productionAirway fibrosisNeutrophil accumulationAirway remodelingSubepithelial fibrosisGob-5Levels of mRNAMetaplasiaInflammationTransgenic miceFibrosisSTAT-6Effector propertiesTransgenic overexpressionFactor productionMiceInterleukinMultiple mechanisms
1996
Association of RNase mitochondrial RNA processing enzyme with ribonuclease P in higher ordered structures in the nucleolus: a possible coordinate role in ribosome biogenesis.
Lee B, Matera AG, Ward DC, Craft J. Association of RNase mitochondrial RNA processing enzyme with ribonuclease P in higher ordered structures in the nucleolus: a possible coordinate role in ribosome biogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 11471-11476. PMID: 8876159, PMCID: PMC38081, DOI: 10.1073/pnas.93.21.11471.Peer-Reviewed Original ResearchConceptsMitochondrial RNA processing enzymeRibosomal RNA maturationRNA processing enzymesRNA maturationRibonuclease PRNase PProcessing enzymesSitu hybridization experimentsRNA structural motifsRibosome biogenesisRibosome assemblySmall subpopulationRibonucleoprotein particleRNA componentMacromolecular complexesRNA transcriptsHybridization experimentsFractionation experimentsCoordinate roleCytoplasmic structuresAffinity selectionEnzymeStructural motifsMethyl oligoribonucleotideNucleoliAutoimmune lpr/lpr mice deficient in CD40 ligand: spontaneous Ig class switching with dichotomy of autoantibody responses.
Ma J, Xu J, Madaio MP, Peng Q, Zhang J, Grewal IS, Flavell RA, Craft J. Autoimmune lpr/lpr mice deficient in CD40 ligand: spontaneous Ig class switching with dichotomy of autoantibody responses. The Journal Of Immunology 1996, 157: 417-26. PMID: 8683147, DOI: 10.4049/jimmunol.157.1.417.Peer-Reviewed Original ResearchConceptsLpr/lpr miceLpr miceIgG autoantibodiesAutoantibody responseCD40 ligandAutoimmune lpr/lpr miceMRL/Mp-lpr/lpr miceHuman systemic lupus erythematosusSystemic lupus erythematosusAge 3 moIgG rheumatoid factorB cell contactSubset of animalsOvert glomerulonephritisLupus erythematosusIgG2b levelsHistologic evidenceRheumatoid factorSerum IgMAb synthesisAutoantibodiesLpr animalsMiceIg classesElevated levelsMHC class I polymorphism in lupus-prone MRL/Mp mice.
Peng SL, Craft J. MHC class I polymorphism in lupus-prone MRL/Mp mice. Immunogenetics 1996, 44: 407-8. PMID: 8781130.Peer-Reviewed Original Research
1995
T cells with gamma/delta T cell receptors (TCR) of intestinal type are preferentially expanded in TCR-alpha-deficient lpr mice.
Hughes DP, Hayday A, Craft JE, Owen MJ, Crispe IN. T cells with gamma/delta T cell receptors (TCR) of intestinal type are preferentially expanded in TCR-alpha-deficient lpr mice. Journal Of Experimental Medicine 1995, 182: 233-241. PMID: 7540652, PMCID: PMC2192080, DOI: 10.1084/jem.182.1.233.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, SurfaceApoptosisBase SequenceCell DivisionCell MovementDNA Transposable ElementsFas ReceptorImmunophenotypingIntestinal MucosaLymph NodesLymphocyte CountLymphoproliferative DisordersMiceMice, KnockoutMice, Mutant StrainsMolecular Sequence DataReceptors, Antigen, T-Cell, alpha-betaReceptors, Antigen, T-Cell, gamma-deltaSpecific Pathogen-Free OrganismsT-Lymphocyte SubsetsConceptsLpr/lpr miceT cell receptorIntestinal intraepithelial lymphocytesLpr miceT cellsIntraepithelial lymphocytesAlpha/beta T cellsGamma/delta T cell receptorGamma/delta T cellsLymph node cell numberTCR gamma/deltaDelta T-cell receptorFas-deficient lpr micePeripheral lymphoid systemT-cell contributionPeripheral lymph nodesBeta T cellsDelta T cellsActivation-induced cell deathGene-ablated miceRole of FasT cell developmentLymph nodesIntestinal typeLymphoid system
1993
Nucleotide sequence analysis of the A protein of the U1 small nuclear ribonucleoprotein particle: the murine protein contains a 5' amino-terminal tag
Bennett M, Baron M, Craft J. Nucleotide sequence analysis of the A protein of the U1 small nuclear ribonucleoprotein particle: the murine protein contains a 5' amino-terminal tag. Nucleic Acids Research 1993, 21: 4404-4404. PMID: 8415008, PMCID: PMC310090, DOI: 10.1093/nar/21.18.4404.Peer-Reviewed Original ResearchBinding of Ku protein to DNA. Measurement of affinity for ends and demonstration of binding to nicks.
Blier PR, Griffith AJ, Craft J, Hardin JA. Binding of Ku protein to DNA. Measurement of affinity for ends and demonstration of binding to nicks. Journal Of Biological Chemistry 1993, 268: 7594-7601. PMID: 8463290, DOI: 10.1016/s0021-9258(18)53216-6.Peer-Reviewed Original Research
1992
The murine gene encoding the highly conserved Sm B protein contains a nonfunctional alternative 3′ splice site
Griffith A, Schmauss C, Craft J. The murine gene encoding the highly conserved Sm B protein contains a nonfunctional alternative 3′ splice site. Gene 1992, 114: 195-201. PMID: 1376292, DOI: 10.1016/0378-1119(92)90574-9.Peer-Reviewed Original ResearchConceptsSplice site consensus sequenceAlternative splice sitesSplice siteGenomic sequencesSplice junctionsGenomic nucleotide sequencesAmino acid sequenceSite consensus sequencePutative branch pointGenomic lociMurine geneMurine mRNAHuman genesSm polypeptidesB geneNucleotide sequenceB polypeptidesB proteinAcid sequenceConsensus sequenceExon sequencesHuman cellsSecondary structureMurine tissuesGenesNucleotide sequence and genomic structure analyses of the p70 subunit of the human Ku autoantigen: evidence for a family of genes encoding Ku (p70)-related polypeptides
Griffith A, Craft J, Evans J, Mimori T, Hardin J. Nucleotide sequence and genomic structure analyses of the p70 subunit of the human Ku autoantigen: evidence for a family of genes encoding Ku (p70)-related polypeptides. Molecular Biology Reports 1992, 16: 91-97. PMID: 1608402, DOI: 10.1007/bf00419754.Peer-Reviewed Original ResearchConceptsHuman Ku autoantigenKu autoantigenGenomic structure analysisFamily of genesHomologous DNA sequencesOpen reading frameEntire open reading frameNucleotide sequence analysisAmino acid polymorphismsSouthern blot analysisP70 polypeptidesHuman genomeReading frameDNA sequencesNucleotide sequenceP70 subunitMultiple genesExpression analysisSequence analysisAcid polymorphismsMultiple copiesBlot analysisPolypeptideGenesSequence
1990
New RNPs of higher eukaryotes
Craft J, Gold H. New RNPs of higher eukaryotes. Molecular Biology Reports 1990, 14: 97-101. PMID: 2194112, DOI: 10.1007/bf00360432.Peer-Reviewed Original ResearchsnRNPs and scRNPs as autoantigens: clues to the etiology of the connective tissue diseases
Craft J, Mamula M, Ohosone Y, Boire G, Gold H, Hardin J. snRNPs and scRNPs as autoantigens: clues to the etiology of the connective tissue diseases. Clinical Rheumatology 1990, 9: 10-19. PMID: 1697517, DOI: 10.1007/bf02205547.Peer-Reviewed Original Research
1989
An immunological determinant of RNase P protein is conserved between Escherichia coli and humans.
Mamula MJ, Baer M, Craft J, Altman S. An immunological determinant of RNase P protein is conserved between Escherichia coli and humans. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 8717-8721. PMID: 2479027, PMCID: PMC298359, DOI: 10.1073/pnas.86.22.8717.Peer-Reviewed Original ResearchThe RNA Processing Enzyme RNase MRP Is Identical to the Th RNP and Related to RNase P
Gold H, Topper J, Clayton D, Craft J. The RNA Processing Enzyme RNase MRP Is Identical to the Th RNP and Related to RNase P. Science 1989, 245: 1377-1380. PMID: 2476849, DOI: 10.1126/science.2476849.Peer-Reviewed Original ResearchConceptsRNA processing enzymesRNase MRPMitochondrial RNA processing enzymeProcessing enzymesRNase MRP activityHuman cell extractsNucleotide sequence analysisRNP enzymeEukaryotic cellsRibonuclease PBiochemical purificationRNase PRNA componentRNA transcriptsSequence analysisCell extractsRNPMRP activityEnzymeMRPTerminusMitochondriaTranscriptsPolypeptideBindsMolecular cloning of cDNA encoding Sm autoantigen: derivation of a cDNA for a B polypeptide of the U series of small nuclear ribonucleoprotein particles.
Ohosone Y, Mimori T, Griffith A, Akizuki M, Homma M, Craft J, Hardin JA. Molecular cloning of cDNA encoding Sm autoantigen: derivation of a cDNA for a B polypeptide of the U series of small nuclear ribonucleoprotein particles. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 4249-4253. PMID: 2524838, PMCID: PMC287428, DOI: 10.1073/pnas.86.11.4249.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusSm snRNPsHuman fibroblast cDNA libraryAmino acidsAlternative splicing mechanismLupus erythematosusFibroblast cDNA librarySmall nuclear ribonucleoproteinOpen reading frameAmino acid sequenceSplicing mechanismMolecular cloningCDNA libraryNuclear ribonucleoproteinReading frameAnti-Sm antibodiesB polypeptidesAcid sequenceCOOH terminusNH2 terminusCDNAComplete homologySnRNPsPolypeptide NPolypeptide