1993
Characterization of distinct functional domains of transforming growth factor beta.
Burmester J, Qian S, Roberts A, Huang A, Amatayakul-Chantler S, Suardet L, Odartchenko N, Madri J, Sporn M. Characterization of distinct functional domains of transforming growth factor beta. Proceedings Of The National Academy Of Sciences Of The United States Of America 1993, 90: 8628-8632. PMID: 7690965, PMCID: PMC47411, DOI: 10.1073/pnas.90.18.8628.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAlpha-MacroglobulinsAmino Acid SequenceAnimalsCattleCHO CellsCricetinaeDose-Response Relationship, DrugEndothelium, VascularHeartHumansKineticsMiceMolecular Sequence DataReceptors, Cell SurfaceReceptors, Transforming Growth Factor betaRecombinant Fusion ProteinsRecombinant ProteinsTransforming Growth Factor betaUmbilical VeinsConceptsGrowth factor betaFactor betaHuman colorectal cancer cell linesColorectal cancer cell linesLS513 cellsCancer cell linesTGF-beta moleculesCell linesAlphaBiological potencyBetaCellsCertain cellsAmino acidsPotencyDistinct functional domainsAdditional amino acidsDifferent isoformsIsoformsDistinct isoformsFunctional domainsChimeric protein
1992
Acidic fibroblast growth factor-Pseudomonas exotoxin chimeric protein elicits antiangiogenic effects on endothelial cells.
Merwin J, Lynch M, Madri J, Pastan I, Siegall C. Acidic fibroblast growth factor-Pseudomonas exotoxin chimeric protein elicits antiangiogenic effects on endothelial cells. Cancer Research 1992, 52: 4995-5001. PMID: 1381275.Peer-Reviewed Original ResearchMeSH KeywordsADP Ribose TransferasesAmino Acid SequenceAnimalsBacterial ToxinsCell MovementCell SurvivalCells, CulturedEndothelium, VascularExotoxinsFibroblast Growth Factor 1In Vitro TechniquesMolecular Sequence DataNeovascularization, PathologicRatsRats, Inbred StrainsReceptors, Cell SurfaceReceptors, Fibroblast Growth FactorRecombinant Fusion ProteinsVirulence FactorsConceptsGrowth factor beta 1Endothelial cellsChimeric toxinBeta 1Inhibitory effectDose-dependent toxic effectAnti-angiogenic agentsMicrovascular endothelial cellsAcidic fibroblast growth factorFibroblast growth factorSignificant inhibitory effectMicrovascular endotheliumAntiangiogenic effectsPseudomonas exotoxinTumor cell linesAntimigratory effectsGrowth factorAngiogenic responseProtein synthesisCell linesFGF receptorsToxic effectsCell deathEndotheliumCell viability analysis
1991
Vascular cells respond differentially to transforming growth factors beta 1 and beta 2 in vitro.
Merwin J, Newman W, Beall L, Tucker A, Madri J. Vascular cells respond differentially to transforming growth factors beta 1 and beta 2 in vitro. American Journal Of Pathology 1991, 138: 37-51. PMID: 1846264, PMCID: PMC1886039.Peer-Reviewed Original ResearchConceptsBovine aortic endothelial cellsBovine aortic smooth muscle cellsGrowth factorGrowth factor beta 1Aortic smooth muscle cellsTGF-beta concentrationsSmooth muscle cellsProliferation of BAECsTGF-beta receptorsBovine aortic endothelial cell migrationType IAortic endothelial cellsTGF beta sCell typesMicrovascular endotheliumVascular cell typesReceptor assayMuscle cellsVascular cellsEndothelial cellsBeta 1Endothelial cell migrationBeta 2Angiogenic assaysBASMCs
1990
A 48 kDa collagen-binding phosphoprotein isolated from bovine aortic endothelial cells interacts with the collagenous domain, but not the globular domain, of collagen type IV
Yannariello-Brown J, Madri J. A 48 kDa collagen-binding phosphoprotein isolated from bovine aortic endothelial cells interacts with the collagenous domain, but not the globular domain, of collagen type IV. Biochemical Journal 1990, 265: 383-392. PMID: 2154186, PMCID: PMC1136898, DOI: 10.1042/bj2650383.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell MembraneCells, CulturedChromatography, AffinityChymotrypsinCollagenElectrophoresis, Gel, Two-DimensionalElectrophoresis, Polyacrylamide GelEndothelium, VascularFluorescent Antibody TechniqueImmune SeraMolecular WeightPeptide MappingPhosphoproteinsReceptors, Cell SurfaceReceptors, CollagenConceptsBovine aortic endothelial cellsSDS/PAGENon-equilibrium pH gel electrophoresisCollagen type IVCollagenous domainTwo-dimensional gel systemAortic endothelial cellsAdhesion assaysCell surface populationIndirect immunofluorescence experimentsGlobular NC1 domainCell surface labelingCollagen-binding proteinsChymotryptic peptide mapsEndothelial cellsGlobular domainIntracellular transportMinor isoformIndividual isoformsImmunofluorescence experimentsMolecular massGolgi regionCollagenous regionCell surfacePeptide maps